Mutagenetix > Incidental Mutation

Incidental Mutation 'R2137:Kng2'

235914

Institutional Source

Beutler Lab

Gene Symbol

Kng2

Ensembl Gene

ENSMUSG00000060459

Gene Name

kininogen 2

Synonyms

Kininogen-II

MMRRC Submission

040140-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2137 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

22804602-22847851 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

G to T at 22816076 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039338] [ENSMUST00000100046] [ENSMUST00000115349] [ENSMUST00000160243]

AlphaFold

Q6S9I3

Predicted Effect

probably benign
Transcript: ENSMUST00000039338

SMART Domains

Protein: ENSMUSP00000046867
Gene: ENSMUSG00000060459

Domain	Start	End	E-Value	Type
CY	18	126	3.72e-19	SMART
CY	140	248	2.57e-22	SMART
CY	262	370	2.06e-35	SMART
low complexity region	376	390	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100046

SMART Domains

Protein: ENSMUSP00000097623
Gene: ENSMUSG00000060459

Domain	Start	End	E-Value	Type
CY	18	126	3.72e-19	SMART
CY	140	248	2.57e-22	SMART
CY	262	370	2.06e-35	SMART
low complexity region	376	390	N/A	INTRINSIC
low complexity region	478	506	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000115349

SMART Domains

Protein: ENSMUSP00000111006
Gene: ENSMUSG00000060459

Domain	Start	End	E-Value	Type
CY	18	126	3.72e-19	SMART
CY	140	248	2.57e-22	SMART
CY	262	370	2.06e-35	SMART
low complexity region	376	390	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000160243

SMART Domains

Protein: ENSMUSP00000124161
Gene: ENSMUSG00000060459

Domain	Start	End	E-Value	Type
CY	18	126	3.72e-19	SMART
Blast:CY	140	171	2e-15	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000231872

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.4%

Validation Efficiency

97% (60/62)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd24	C	T	10: 81,482,143 (GRCm39)	T88I	probably damaging	Het
Atm	A	T	9: 53,364,675 (GRCm39)	V49D	probably damaging	Het
Bub1b	G	T	2: 118,467,199 (GRCm39)	E841*	probably null	Het
Cdh20	A	T	1: 110,027,836 (GRCm39)	N527I	probably damaging	Het
Cdh22	G	A	2: 164,958,314 (GRCm39)		probably benign	Het
Cog1	T	C	11: 113,550,127 (GRCm39)	L262P	probably damaging	Het
Col22a1	T	C	15: 71,878,797 (GRCm39)	H120R	possibly damaging	Het
Col4a2	T	C	8: 11,483,749 (GRCm39)	S890P	probably benign	Het
Cubn	T	C	2: 13,340,978 (GRCm39)	I2248V	probably benign	Het
Evpl	T	C	11: 116,112,665 (GRCm39)	E1675G	probably damaging	Het
Faiml	G	A	9: 99,114,545 (GRCm39)	P115S	probably benign	Het
Fgg	A	T	3: 82,915,745 (GRCm39)	D62V	possibly damaging	Het
Gak	C	T	5: 108,754,743 (GRCm39)		probably null	Het
Galntl6	C	T	8: 58,988,939 (GRCm39)		probably null	Het
Glyr1	T	C	16: 4,836,346 (GRCm39)	Y501C	probably benign	Het
Gm9847	G	T	12: 14,545,082 (GRCm39)		noncoding transcript	Het
Gria4	T	G	9: 4,427,026 (GRCm39)		probably benign	Het
Il36b	A	G	2: 24,044,672 (GRCm39)	N24S	probably benign	Het
Il6st	C	A	13: 112,639,392 (GRCm39)	H606N	possibly damaging	Het
Itprid1	A	G	6: 55,866,174 (GRCm39)	Q189R	probably damaging	Het
Kctd10	A	G	5: 114,505,389 (GRCm39)	F202L	probably damaging	Het
Kif17	T	A	4: 137,989,978 (GRCm39)	D55E	probably damaging	Het
Klf1	C	T	8: 85,629,775 (GRCm39)	A200V	possibly damaging	Het
Klhl32	A	T	4: 24,629,275 (GRCm39)	Y497*	probably null	Het
Lats1	T	C	10: 7,577,611 (GRCm39)	V245A	possibly damaging	Het
Mbd3l2	A	T	9: 18,356,254 (GRCm39)	D193V	probably damaging	Het
Ms4a18	A	T	19: 10,974,695 (GRCm39)	V332D	possibly damaging	Het
Mss51	T	C	14: 20,537,591 (GRCm39)	I47V	probably benign	Het
Myoz2	G	A	3: 122,827,861 (GRCm39)	T19M	probably benign	Het
Nampt	T	A	12: 32,880,309 (GRCm39)	N67K	probably benign	Het
Ncor2	A	T	5: 125,107,776 (GRCm39)	I1607K	probably damaging	Het
Nudt4	T	C	10: 95,399,600 (GRCm39)	Q7R	probably damaging	Het
Or5b119	A	G	19: 13,457,173 (GRCm39)	Y130H	probably damaging	Het
Or5b121	T	A	19: 13,507,802 (GRCm39)	I255N	probably damaging	Het
Or8b12i	T	A	9: 20,082,463 (GRCm39)	I135F	probably damaging	Het
Pgm2	A	T	5: 64,273,709 (GRCm39)	M565L	probably benign	Het
Phactr1	T	G	13: 43,288,651 (GRCm39)	F639V	possibly damaging	Het
Plod3	C	A	5: 137,017,571 (GRCm39)	R165S	probably damaging	Het
Polr2b	T	A	5: 77,468,193 (GRCm39)	N164K	probably benign	Het
Rcbtb2	G	A	14: 73,399,491 (GRCm39)	G52S	probably damaging	Het
Rfc1	A	G	5: 65,468,382 (GRCm39)		probably null	Het
Rheb	A	G	5: 25,012,601 (GRCm39)		probably null	Het
Rin2	C	T	2: 145,702,366 (GRCm39)	T354I	probably benign	Het
Ripor2	G	A	13: 24,905,817 (GRCm39)		probably null	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Scg3	A	T	9: 75,584,092 (GRCm39)	D136E	probably damaging	Het
Slc10a4	C	T	5: 73,164,923 (GRCm39)	L172F	probably damaging	Het
Slc35c2	A	G	2: 165,123,299 (GRCm39)	Y113H	probably damaging	Het
Slc47a1	T	A	11: 61,235,318 (GRCm39)	D505V	probably benign	Het
Snap29	C	A	16: 17,246,113 (GRCm39)	D244E	possibly damaging	Het
Taar1	T	C	10: 23,797,168 (GRCm39)	F289L	probably benign	Het
Thbs2	T	A	17: 14,893,568 (GRCm39)	N871Y	probably damaging	Het
Tmem108	T	A	9: 103,377,162 (GRCm39)	T96S	possibly damaging	Het
Tnk2	G	T	16: 32,489,620 (GRCm39)		probably null	Het
Trak1	T	A	9: 121,302,028 (GRCm39)	M928K	possibly damaging	Het
Tuba3b	A	G	6: 145,564,559 (GRCm39)	I110V	probably benign	Het
Tyk2	A	G	9: 21,022,281 (GRCm39)		probably benign	Het
Ugt1a9	T	A	1: 87,998,759 (GRCm39)	C70S	probably benign	Het
Vmn2r10	A	T	5: 109,151,410 (GRCm39)	I68K	possibly damaging	Het
Wfs1	T	C	5: 37,124,845 (GRCm39)	E682G	probably damaging	Het
Zfp213	T	C	17: 23,778,481 (GRCm39)		probably null	Het
Zfp809	T	C	9: 22,146,434 (GRCm39)	V41A	probably benign	Het
Zfp831	A	G	2: 174,547,539 (GRCm39)	K1574R	possibly damaging	Het

Other mutations in Kng2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00851:Kng2	APN	16	22,847,580 (GRCm39)	missense	probably damaging	1.00
IGL01319:Kng2	APN	16	22,847,584 (GRCm39)	missense	probably damaging	0.99
IGL01469:Kng2	APN	16	22,818,577 (GRCm39)	missense	probably damaging	1.00
IGL01681:Kng2	APN	16	22,815,767 (GRCm39)	splice site	probably benign
IGL01830:Kng2	APN	16	22,806,801 (GRCm39)	missense	probably damaging	0.97
IGL01903:Kng2	APN	16	22,806,540 (GRCm39)	missense	possibly damaging	0.80
IGL02146:Kng2	APN	16	22,806,582 (GRCm39)	missense	probably damaging	0.97
IGL02305:Kng2	APN	16	22,819,374 (GRCm39)	unclassified	probably benign
IGL02429:Kng2	APN	16	22,830,829 (GRCm39)	missense	probably damaging	0.99
FR4548:Kng2	UTSW	16	22,819,302 (GRCm39)	nonsense	probably null
R0020:Kng2	UTSW	16	22,816,046 (GRCm39)	missense	probably benign	0.02
R0047:Kng2	UTSW	16	22,806,313 (GRCm39)	missense	possibly damaging	0.83
R0245:Kng2	UTSW	16	22,830,931 (GRCm39)	splice site	probably benign
R0610:Kng2	UTSW	16	22,819,344 (GRCm39)	missense	possibly damaging	0.96
R0646:Kng2	UTSW	16	22,806,486 (GRCm39)	missense	probably benign	0.10
R0666:Kng2	UTSW	16	22,815,872 (GRCm39)	splice site	probably benign
R1552:Kng2	UTSW	16	22,806,270 (GRCm39)	missense	probably damaging	1.00
R1765:Kng2	UTSW	16	22,806,993 (GRCm39)	critical splice donor site	probably null
R1833:Kng2	UTSW	16	22,830,802 (GRCm39)	missense	possibly damaging	0.95
R1997:Kng2	UTSW	16	22,843,626 (GRCm39)	missense	possibly damaging	0.84
R2025:Kng2	UTSW	16	22,819,325 (GRCm39)	missense	probably benign	0.15
R2056:Kng2	UTSW	16	22,806,703 (GRCm39)	intron	probably benign
R2517:Kng2	UTSW	16	22,807,065 (GRCm39)	missense	probably benign	0.24
R3438:Kng2	UTSW	16	22,830,821 (GRCm39)	missense	probably benign	0.23
R3439:Kng2	UTSW	16	22,830,821 (GRCm39)	missense	probably benign	0.23
R3551:Kng2	UTSW	16	22,830,745 (GRCm39)	critical splice donor site	probably null
R4389:Kng2	UTSW	16	22,843,618 (GRCm39)	missense	possibly damaging	0.91
R4538:Kng2	UTSW	16	22,806,813 (GRCm39)	missense	probably benign	0.00
R4684:Kng2	UTSW	16	22,806,391 (GRCm39)	missense	possibly damaging	0.93
R4978:Kng2	UTSW	16	22,806,666 (GRCm39)	missense	probably damaging	1.00
R5658:Kng2	UTSW	16	22,815,770 (GRCm39)	splice site	probably null
R6074:Kng2	UTSW	16	22,819,346 (GRCm39)	missense	probably benign	0.03
R6216:Kng2	UTSW	16	22,806,343 (GRCm39)	missense	probably damaging	1.00
R6271:Kng2	UTSW	16	22,822,698 (GRCm39)	missense	probably benign	0.33
R6459:Kng2	UTSW	16	22,830,865 (GRCm39)	missense	probably damaging	1.00
R7124:Kng2	UTSW	16	22,830,805 (GRCm39)	missense	probably damaging	1.00
R7310:Kng2	UTSW	16	22,806,522 (GRCm39)	missense	probably benign	0.00
R7532:Kng2	UTSW	16	22,845,794 (GRCm39)	splice site	probably null
R7667:Kng2	UTSW	16	22,806,982 (GRCm39)	missense	probably damaging	0.99
R7787:Kng2	UTSW	16	22,818,598 (GRCm39)	missense	probably damaging	1.00
R8092:Kng2	UTSW	16	22,806,672 (GRCm39)	missense	probably benign	0.00
R8165:Kng2	UTSW	16	22,806,246 (GRCm39)	missense	unknown
R8814:Kng2	UTSW	16	22,822,761 (GRCm39)	missense	probably benign	0.00
R9019:Kng2	UTSW	16	22,847,546 (GRCm39)	missense	probably damaging	0.99
R9048:Kng2	UTSW	16	22,806,522 (GRCm39)	missense	probably benign	0.00
R9531:Kng2	UTSW	16	22,830,907 (GRCm39)	missense	possibly damaging	0.81
R9708:Kng2	UTSW	16	22,815,801 (GRCm39)	missense	probably damaging	0.99
R9764:Kng2	UTSW	16	22,822,737 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACGTTAGCATTGCAGTCGAG -3'
(R):5'- GTGCACCTTGTAGAGAATTTTGTAC -3'

Sequencing Primer
(F):5'- TCAGCATTAGTCATGAGGCC -3'
(R):5'- AGAGAATTTTGTACTTGTAGTCACAC -3'

Posted On

2014-10-01