Mutagenetix > Incidental Mutation

Incidental Mutation 'R2138:Mov10'

235936

Institutional Source

Beutler Lab

Gene Symbol

Mov10

Ensembl Gene

ENSMUSG00000002227

Gene Name

Moloney leukemia virus 10

Synonyms

Mov-10

MMRRC Submission

040141-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.289) question?

Stock #

R2138 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

104794836-104818563 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 104804242 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 316 (H316R)

Ref Sequence

ENSEMBL: ENSMUSP00000126897 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000002297] [ENSMUST00000106774] [ENSMUST00000106775] [ENSMUST00000136148] [ENSMUST00000166979] [ENSMUST00000168015]

AlphaFold

P23249

Predicted Effect

probably benign
Transcript: ENSMUST00000002297
AA Change: H243R

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000002297
Gene: ENSMUSG00000002227
AA Change: H243R

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106774

SMART Domains

Protein: ENSMUSP00000102386
Gene: ENSMUSG00000002227

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106775
AA Change: H316R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000102387
Gene: ENSMUSG00000002227
AA Change: H316R

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000136148

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145414

Predicted Effect

probably benign
Transcript: ENSMUST00000166979
AA Change: H316R

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000126897
Gene: ENSMUSG00000002227
AA Change: H316R

Domain	Start	End	E-Value	Type
low complexity region	61	75	N/A	INTRINSIC
low complexity region	370	385	N/A	INTRINSIC
low complexity region	411	426	N/A	INTRINSIC
AAA	590	772	5.72e-3	SMART
low complexity region	1026	1043	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000168015
AA Change: H243R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000128246
Gene: ENSMUSG00000002227
AA Change: H243R

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196161

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196211

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous knockout is embryonic lethal. Heterozygous knockout leads to reduced dendritic branching of neurons, which affects anxiety- and/or activity-related behavior. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aamp	A	G	1: 74,283,963	V6A	probably benign	Het
Abcc6	A	G	7: 45,981,051	F1262L	probably damaging	Het
Acot13	A	T	13: 24,818,205		probably null	Het
Adgrv1	T	C	13: 81,445,320	I4183V	probably benign	Het
Aff4	T	G	11: 53,372,512	S120A	possibly damaging	Het
Afp	A	G	5: 90,499,647	E250G	probably damaging	Het
Ankrd34b	A	T	13: 92,439,406	D382V	probably damaging	Het
Arhgef19	A	T	4: 141,250,800	I577F	probably damaging	Het
Arl6	A	G	16: 59,622,467		probably benign	Het
Atp2b2	A	T	6: 113,796,307	M333K	probably benign	Het
Atp8b3	C	A	10: 80,527,105	A635S	possibly damaging	Het
Baiap2	A	G	11: 119,957,102	T19A	possibly damaging	Het
Bcar3	A	G	3: 122,512,996	D206G	probably damaging	Het
Ccdc162	A	T	10: 41,581,297	M85K	probably benign	Het
Clec4a4	A	G	6: 123,023,978	N217D	probably damaging	Het
Csmd1	T	C	8: 15,929,088	Y2832C	probably damaging	Het
Cubn	A	G	2: 13,444,378	I962T	probably damaging	Het
Dennd4a	A	G	9: 64,889,337	Y852C	probably damaging	Het
Dhcr24	G	T	4: 106,572,302	E191*	probably null	Het
Dusp12	G	A	1: 170,880,597	Q114*	probably null	Het
Elfn2	G	T	15: 78,674,038	T103K	probably benign	Het
Eme1	A	T	11: 94,648,192	V314E	probably damaging	Het
Epb41l3	A	G	17: 69,207,880	E4G	probably damaging	Het
Exoc6b	A	T	6: 84,989,482	L170Q	probably damaging	Het
Fam129a	G	A	1: 151,696,251	V316M	probably damaging	Het
Fbln5	C	T	12: 101,761,920	M261I	probably benign	Het
Fgf22	T	C	10: 79,756,601	V64A	probably damaging	Het
Gak	C	T	5: 108,606,877		probably null	Het
Gatad2b	T	A	3: 90,352,113	S401R	probably damaging	Het
Gen1	A	T	12: 11,241,621	S722R	probably damaging	Het
Gm10754	A	T	10: 97,682,270		probably benign	Het
Gm8897	A	T	5: 11,419,085	R68*	probably null	Het
Grid2	G	A	6: 64,345,798	R594Q	probably damaging	Het
Grm7	C	A	6: 110,646,137	N90K	probably damaging	Het
Herc1	G	A	9: 66,470,307	V3452M	possibly damaging	Het
Il22ra2	T	A	10: 19,632,870	F215L	probably benign	Het
Kank1	G	A	19: 25,411,753	G930D	probably benign	Het
Klra3	A	T	6: 130,333,158	V133D	probably benign	Het
Lims2	A	G	18: 31,955,407	E220G	possibly damaging	Het
Mbd3l2	A	T	9: 18,444,958	D193V	probably damaging	Het
Mbl1	T	A	14: 41,153,691	I34K	possibly damaging	Het
Mgam	C	T	6: 40,756,450	P839S	probably damaging	Het
Mmp9	G	T	2: 164,952,467	E460*	probably null	Het
Ms4a18	A	T	19: 10,997,331	V332D	possibly damaging	Het
Myo15b	T	C	11: 115,883,807	S2082P	probably benign	Het
Myrfl	G	A	10: 116,795,538	T706I	probably benign	Het
Nampt	C	A	12: 32,838,422	H191N	possibly damaging	Het
Nphp3	A	G	9: 104,025,903	E693G	possibly damaging	Het
Obscn	A	T	11: 59,003,665	Y1191*	probably null	Het
Olfr19	A	G	16: 16,673,205	Y259H	probably damaging	Het
Olfr398	A	T	11: 73,984,303	Y102N	probably damaging	Het
Olfr419	A	T	1: 174,250,736		probably null	Het
Osbpl10	A	G	9: 115,232,134	N760S	probably benign	Het
Otof	A	G	5: 30,461,770	V10A	probably benign	Het
Pkhd1l1	A	C	15: 44,501,457	E664A	probably damaging	Het
Pnp2	T	C	14: 50,963,704	S178P	probably damaging	Het
Pvr	G	A	7: 19,917,002	T199I	probably damaging	Het
Rbp3	C	T	14: 33,956,018	T641M	probably damaging	Het
Rnaseh2b	T	C	14: 62,361,345	V173A	probably benign	Het
Sept12	C	T	16: 4,992,206	R155H	probably damaging	Het
Slco6d1	G	T	1: 98,443,660	R290L	probably benign	Het
Snx32	A	G	19: 5,496,129	V335A	probably damaging	Het
Son	T	C	16: 91,659,372	V1669A	possibly damaging	Het
Tbata	C	T	10: 61,179,284	T116I	probably benign	Het
Tdrd1	T	A	19: 56,842,589	S279T	probably benign	Het
Thsd7a	A	T	6: 12,471,073	Y515*	probably null	Het
Tmem102	T	G	11: 69,805,114	L40F	probably damaging	Het
Tmem169	A	G	1: 72,300,996	N195S	probably damaging	Het
Tmem201	A	T	4: 149,718,080	S613T	probably damaging	Het
Tnfaip6	A	T	2: 52,052,332	I218F	possibly damaging	Het
Tube1	C	A	10: 39,147,351	H331Q	probably benign	Het
Wwc1	G	A	11: 35,841,887	T998I	possibly damaging	Het
Xpc	A	T	6: 91,498,122	Y638*	probably null	Het
Zdhhc4	A	T	5: 143,324,262	Y80*	probably null	Het

Other mutations in Mov10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00498:Mov10	APN	3	104800947	splice site	probably benign
IGL01111:Mov10	APN	3	104801405	missense	possibly damaging	0.71
IGL01315:Mov10	APN	3	104795945	missense	probably damaging	0.98
IGL01463:Mov10	APN	3	104800324	missense	probably damaging	1.00
IGL02114:Mov10	APN	3	104795318	unclassified	probably benign
IGL02354:Mov10	APN	3	104804121	splice site	probably benign
IGL02361:Mov10	APN	3	104804121	splice site	probably benign
IGL02692:Mov10	APN	3	104800803	nonsense	probably null
IGL03104:Mov10	APN	3	104797307	missense	probably damaging	1.00
IGL03121:Mov10	APN	3	104801002	missense	probably benign
P0040:Mov10	UTSW	3	104804679	missense	probably damaging	1.00
R0025:Mov10	UTSW	3	104804603	missense	probably damaging	1.00
R0270:Mov10	UTSW	3	104795405	missense	probably benign	0.09
R0747:Mov10	UTSW	3	104802496	missense	probably benign	0.41
R1434:Mov10	UTSW	3	104795174	missense	probably damaging	1.00
R1482:Mov10	UTSW	3	104804546	missense	probably damaging	0.98
R1594:Mov10	UTSW	3	104795411	missense	probably damaging	1.00
R1656:Mov10	UTSW	3	104799596	missense	probably benign	0.03
R1739:Mov10	UTSW	3	104800282	missense	probably damaging	0.98
R1785:Mov10	UTSW	3	104818116	missense	possibly damaging	0.73
R1786:Mov10	UTSW	3	104818116	missense	possibly damaging	0.73
R1911:Mov10	UTSW	3	104801560	splice site	probably benign
R1962:Mov10	UTSW	3	104796977	missense	probably damaging	1.00
R1993:Mov10	UTSW	3	104799419	missense	probably damaging	1.00
R2095:Mov10	UTSW	3	104801531	missense	probably damaging	1.00
R3107:Mov10	UTSW	3	104799724	missense	probably damaging	1.00
R4241:Mov10	UTSW	3	104797276	missense	probably benign	0.45
R4280:Mov10	UTSW	3	104799779	missense	probably damaging	0.98
R4474:Mov10	UTSW	3	104818465	missense	probably damaging	1.00
R5227:Mov10	UTSW	3	104802578	missense	probably benign
R5391:Mov10	UTSW	3	104802533	missense	probably benign	0.12
R5704:Mov10	UTSW	3	104799596	missense	probably benign	0.03
R5819:Mov10	UTSW	3	104801512	missense	probably damaging	1.00
R5842:Mov10	UTSW	3	104799379	splice site	probably benign
R6059:Mov10	UTSW	3	104817950	utr 3 prime	probably benign
R6692:Mov10	UTSW	3	104818044	missense	probably damaging	0.97
R7226:Mov10	UTSW	3	104801012	missense	probably damaging	1.00
R7426:Mov10	UTSW	3	104800052	splice site	probably null
R7633:Mov10	UTSW	3	104797065	missense	possibly damaging	0.93
R7637:Mov10	UTSW	3	104795885	missense	probably benign	0.26
R7869:Mov10	UTSW	3	104804678	missense	probably damaging	1.00
R8684:Mov10	UTSW	3	104804374	missense	probably benign
R9008:Mov10	UTSW	3	104800016	missense	probably benign	0.09
R9127:Mov10	UTSW	3	104804343	nonsense	probably null
R9559:Mov10	UTSW	3	104800961	missense
R9587:Mov10	UTSW	3	104804583	missense	probably benign	0.11
R9602:Mov10	UTSW	3	104800968	missense	probably benign	0.18
R9606:Mov10	UTSW	3	104800348	missense	probably benign	0.00
R9708:Mov10	UTSW	3	104797297	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACCTTGCTCCTCTGATATGAAAG -3'
(R):5'- AGGTTGCTCCTTCACTCAGC -3'

Sequencing Primer
(F):5'- GCTCCTCTGATATGAAAGGGATG -3'
(R):5'- CAGCTAACTTTTTCCAGGTGAAAGC -3'

Posted On

2014-10-01