Incidental Mutation 'R2138:Klra3'
ID235959
Institutional Source Beutler Lab
Gene Symbol Klra3
Ensembl Gene ENSMUSG00000067591
Gene Namekiller cell lectin-like receptor, subfamily A, member 3
SynonymsNK-2.1, Nk2.1, Ly49C, Nk-2, Nk2, Ly49c, 5E6
MMRRC Submission 040141-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.053) question?
Stock #R2138 (G1)
Quality Score225
Status Not validated
Chromosome6
Chromosomal Location130323289-130337574 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 130333158 bp
ZygosityHeterozygous
Amino Acid Change Valine to Aspartic acid at position 133 (V133D)
Ref Sequence ENSEMBL: ENSMUSP00000107629 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000088017] [ENSMUST00000111998]
Predicted Effect probably benign
Transcript: ENSMUST00000088017
AA Change: V133D

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000085333
Gene: ENSMUSG00000067591
AA Change: V133D

DomainStartEndE-ValueType
low complexity region 56 66 N/A INTRINSIC
Blast:CLECT 73 117 1e-7 BLAST
CLECT 143 258 7.11e-16 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000111998
AA Change: V133D

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000107629
Gene: ENSMUSG00000067591
AA Change: V133D

DomainStartEndE-ValueType
low complexity region 56 66 N/A INTRINSIC
Blast:CLECT 73 117 1e-7 BLAST
CLECT 143 258 7.11e-16 SMART
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.3%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 74 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aamp A G 1: 74,283,963 V6A probably benign Het
Abcc6 A G 7: 45,981,051 F1262L probably damaging Het
Acot13 A T 13: 24,818,205 probably null Het
Adgrv1 T C 13: 81,445,320 I4183V probably benign Het
Aff4 T G 11: 53,372,512 S120A possibly damaging Het
Afp A G 5: 90,499,647 E250G probably damaging Het
Ankrd34b A T 13: 92,439,406 D382V probably damaging Het
Arhgef19 A T 4: 141,250,800 I577F probably damaging Het
Arl6 A G 16: 59,622,467 probably benign Het
Atp2b2 A T 6: 113,796,307 M333K probably benign Het
Atp8b3 C A 10: 80,527,105 A635S possibly damaging Het
Baiap2 A G 11: 119,957,102 T19A possibly damaging Het
Bcar3 A G 3: 122,512,996 D206G probably damaging Het
Ccdc162 A T 10: 41,581,297 M85K probably benign Het
Clec4a4 A G 6: 123,023,978 N217D probably damaging Het
Csmd1 T C 8: 15,929,088 Y2832C probably damaging Het
Cubn A G 2: 13,444,378 I962T probably damaging Het
Dennd4a A G 9: 64,889,337 Y852C probably damaging Het
Dhcr24 G T 4: 106,572,302 E191* probably null Het
Dusp12 G A 1: 170,880,597 Q114* probably null Het
Elfn2 G T 15: 78,674,038 T103K probably benign Het
Eme1 A T 11: 94,648,192 V314E probably damaging Het
Epb41l3 A G 17: 69,207,880 E4G probably damaging Het
Exoc6b A T 6: 84,989,482 L170Q probably damaging Het
Fam129a G A 1: 151,696,251 V316M probably damaging Het
Fbln5 C T 12: 101,761,920 M261I probably benign Het
Fgf22 T C 10: 79,756,601 V64A probably damaging Het
Gak C T 5: 108,606,877 probably null Het
Gatad2b T A 3: 90,352,113 S401R probably damaging Het
Gen1 A T 12: 11,241,621 S722R probably damaging Het
Gm10754 A T 10: 97,682,270 probably benign Het
Gm8897 A T 5: 11,419,085 R68* probably null Het
Grid2 G A 6: 64,345,798 R594Q probably damaging Het
Grm7 C A 6: 110,646,137 N90K probably damaging Het
Herc1 G A 9: 66,470,307 V3452M possibly damaging Het
Il22ra2 T A 10: 19,632,870 F215L probably benign Het
Kank1 G A 19: 25,411,753 G930D probably benign Het
Lims2 A G 18: 31,955,407 E220G possibly damaging Het
Mbd3l2 A T 9: 18,444,958 D193V probably damaging Het
Mbl1 T A 14: 41,153,691 I34K possibly damaging Het
Mgam C T 6: 40,756,450 P839S probably damaging Het
Mmp9 G T 2: 164,952,467 E460* probably null Het
Mov10 T C 3: 104,804,242 H316R probably benign Het
Ms4a18 A T 19: 10,997,331 V332D possibly damaging Het
Myo15b T C 11: 115,883,807 S2082P probably benign Het
Myrfl G A 10: 116,795,538 T706I probably benign Het
Nampt C A 12: 32,838,422 H191N possibly damaging Het
Nphp3 A G 9: 104,025,903 E693G possibly damaging Het
Obscn A T 11: 59,003,665 Y1191* probably null Het
Olfr19 A G 16: 16,673,205 Y259H probably damaging Het
Olfr398 A T 11: 73,984,303 Y102N probably damaging Het
Olfr419 A T 1: 174,250,736 probably null Het
Osbpl10 A G 9: 115,232,134 N760S probably benign Het
Otof A G 5: 30,461,770 V10A probably benign Het
Pkhd1l1 A C 15: 44,501,457 E664A probably damaging Het
Pnp2 T C 14: 50,963,704 S178P probably damaging Het
Pvr G A 7: 19,917,002 T199I probably damaging Het
Rbp3 C T 14: 33,956,018 T641M probably damaging Het
Rnaseh2b T C 14: 62,361,345 V173A probably benign Het
Sept12 C T 16: 4,992,206 R155H probably damaging Het
Slco6d1 G T 1: 98,443,660 R290L probably benign Het
Snx32 A G 19: 5,496,129 V335A probably damaging Het
Son T C 16: 91,659,372 V1669A possibly damaging Het
Tbata C T 10: 61,179,284 T116I probably benign Het
Tdrd1 T A 19: 56,842,589 S279T probably benign Het
Thsd7a A T 6: 12,471,073 Y515* probably null Het
Tmem102 T G 11: 69,805,114 L40F probably damaging Het
Tmem169 A G 1: 72,300,996 N195S probably damaging Het
Tmem201 A T 4: 149,718,080 S613T probably damaging Het
Tnfaip6 A T 2: 52,052,332 I218F possibly damaging Het
Tube1 C A 10: 39,147,351 H331Q probably benign Het
Wwc1 G A 11: 35,841,887 T998I possibly damaging Het
Xpc A T 6: 91,498,122 Y638* probably null Het
Zdhhc4 A T 5: 143,324,262 Y80* probably null Het
Other mutations in Klra3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00836:Klra3 APN 6 130327144 missense probably benign 0.26
R0004:Klra3 UTSW 6 130323687 missense probably damaging 1.00
R1532:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1533:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1534:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1536:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1547:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1548:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1566:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1567:Klra3 UTSW 6 130333144 missense probably benign 0.12
R1769:Klra3 UTSW 6 130330263 critical splice acceptor site probably null
R1772:Klra3 UTSW 6 130323708 missense probably benign
R1806:Klra3 UTSW 6 130327070 missense probably damaging 0.99
R2131:Klra3 UTSW 6 130335775 missense probably benign 0.07
R2152:Klra3 UTSW 6 130333144 missense probably benign 0.12
R2154:Klra3 UTSW 6 130333144 missense probably benign 0.12
R2906:Klra3 UTSW 6 130333339 missense probably damaging 0.99
R2907:Klra3 UTSW 6 130333339 missense probably damaging 0.99
R4287:Klra3 UTSW 6 130334302 missense probably benign 0.08
R4732:Klra3 UTSW 6 130327132 missense possibly damaging 0.94
R4733:Klra3 UTSW 6 130327132 missense possibly damaging 0.94
R4829:Klra3 UTSW 6 130323616 missense probably benign 0.05
R5308:Klra3 UTSW 6 130334307 splice site probably null
R6701:Klra3 UTSW 6 130330253 missense probably benign 0.01
R7019:Klra3 UTSW 6 130327124 missense probably damaging 1.00
R7174:Klra3 UTSW 6 130335978 intron probably null
X0052:Klra3 UTSW 6 130333180 missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- GGAGTGCCAAAGAGAACTTTCC -3'
(R):5'- TGTGTCAGCTCTCCACCTGAAG -3'

Sequencing Primer
(F):5'- GTGCCAAAGAGAACTTTCCTTCTAG -3'
(R):5'- CCACCTGAAGCCTTTTCTCTAAC -3'
Posted On2014-10-01