Mutagenetix > Incidental Mutation

Incidental Mutation 'R2141:Nfasc'

236201

Institutional Source

Beutler Lab

Gene Symbol

Nfasc

Ensembl Gene

ENSMUSG00000026442

Gene Name

neurofascin

Synonyms

D430023G06Rik

MMRRC Submission

040144-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2141 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132492428-132669535 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 132524383 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 932 (P932L)

Ref Sequence

ENSEMBL: ENSMUSP00000139955 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043189] [ENSMUST00000094569] [ENSMUST00000163770] [ENSMUST00000187861] [ENSMUST00000188307]

AlphaFold

Q810U3

Predicted Effect

probably damaging
Transcript: ENSMUST00000043189
AA Change: P911L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000035454
Gene: ENSMUSG00000026442
AA Change: P911L

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	42	131	4.5e0	SMART
IG	141	228	2.44e-7	SMART
IGc2	253	317	1.53e-17	SMART
IGc2	343	409	1.76e-8	SMART
IGc2	437	502	2.39e-10	SMART
IGc2	528	593	2.54e-5	SMART
FN3	607	690	2.17e-11	SMART
FN3	707	789	2.85e-6	SMART
FN3	805	896	2.21e-3	SMART
FN3	911	995	9.92e-6	SMART
low complexity region	996	1018	N/A	INTRINSIC
transmembrane domain	1026	1048	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1049	1133	1.4e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000094569
AA Change: P825L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000092148
Gene: ENSMUSG00000026442
AA Change: P825L

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	48	137	4.5e0	SMART
IG	147	234	2.44e-7	SMART
IGc2	259	323	1.53e-17	SMART
IGc2	349	415	1.76e-8	SMART
IGc2	443	508	2.39e-10	SMART
IGc2	534	599	2.54e-5	SMART
FN3	628	711	2.17e-11	SMART
FN3	728	810	2.85e-6	SMART
FN3	825	909	9.92e-6	SMART
FN3	1010	1086	6.91e-5	SMART
transmembrane domain	1109	1131	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1132	1216	2.2e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000163770
AA Change: P928L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132979
Gene: ENSMUSG00000026442
AA Change: P928L

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	42	131	4.5e0	SMART
IG	141	228	2.44e-7	SMART
IGc2	270	334	1.53e-17	SMART
IGc2	360	426	1.76e-8	SMART
IGc2	454	519	2.39e-10	SMART
IGc2	545	610	2.54e-5	SMART
FN3	624	707	2.17e-11	SMART
FN3	724	806	2.85e-6	SMART
FN3	822	913	2.21e-3	SMART
FN3	928	1012	9.92e-6	SMART
low complexity region	1013	1035	N/A	INTRINSIC
transmembrane domain	1043	1065	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1066	1150	5e-30	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000186389
AA Change: P912L

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186539

Predicted Effect

probably damaging
Transcript: ENSMUST00000187861
AA Change: P932L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139955
Gene: ENSMUSG00000026442
AA Change: P932L

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	48	137	1.8e-2	SMART
IG	147	234	1e-9	SMART
IGc2	259	323	6.4e-20	SMART
IGc2	349	415	7e-11	SMART
IGc2	443	508	9.7e-13	SMART
IGc2	534	599	1.1e-7	SMART
FN3	628	711	1e-13	SMART
FN3	728	810	1.4e-8	SMART
FN3	826	917	1.1e-5	SMART
FN3	932	1016	4.8e-8	SMART
FN3	1117	1193	3.4e-7	SMART
transmembrane domain	1216	1238	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1239	1325	2.6e-26	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188307

SMART Domains

Protein: ENSMUSP00000139520
Gene: ENSMUSG00000026442

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	42	131	1.8e-2	SMART
IG	141	228	1e-9	SMART
IGc2	253	317	6.4e-20	SMART
IGc2	343	409	7e-11	SMART
IGc2	437	502	9.7e-13	SMART
IGc2	528	593	1.1e-7	SMART
FN3	622	705	1e-13	SMART
FN3	722	804	1.4e-8	SMART
FN3	820	890	3.8e-1	SMART

Meta Mutation Damage Score

0.8035

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

97% (107/110)

MGI Phenotype

FUNCTION: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]
PHENOTYPE: Mice homozygous for a null allele die within 6 to 7 days of birth, exhibit reduced nerve conduction velocity and abnormal paranodal junction formation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810065E05Rik	G	T	11: 58,314,752 (GRCm39)	C150F	probably damaging	Het
Aagab	T	A	9: 63,523,957 (GRCm39)		probably null	Het
Abca15	A	C	7: 120,006,697 (GRCm39)	T1654P	probably damaging	Het
Abca17	T	A	17: 24,553,240 (GRCm39)	Y157F	probably benign	Het
Aftph	A	T	11: 20,648,318 (GRCm39)	L813*	probably null	Het
Agap1	T	C	1: 89,765,477 (GRCm39)	I615T	probably damaging	Het
Ak3	T	G	19: 29,000,247 (GRCm39)	Q221H	probably benign	Het
Aldoa	A	G	7: 126,396,814 (GRCm39)		probably null	Het
Anxa8	T	C	14: 33,813,873 (GRCm39)		probably null	Het
Atpsckmt	T	C	15: 31,609,718 (GRCm39)	F146L	probably benign	Het
Baz2a	A	G	10: 127,959,481 (GRCm39)	D1329G	probably damaging	Het
Calhm6	G	T	10: 34,003,691 (GRCm39)	A72E	probably damaging	Het
Capn9	G	A	8: 125,332,450 (GRCm39)	G430R	possibly damaging	Het
Cd55	C	T	1: 130,377,160 (GRCm39)	V333I	probably benign	Het
Cep70	A	G	9: 99,178,438 (GRCm39)	Y512C	probably damaging	Het
Cfhr2	T	G	1: 139,758,893 (GRCm39)	R52S	probably benign	Het
Clcn6	A	G	4: 148,108,594 (GRCm39)	F145S	possibly damaging	Het
Cnksr1	A	G	4: 133,956,939 (GRCm39)	Y488H	probably damaging	Het
Diablo	A	G	5: 123,661,424 (GRCm39)	V24A	probably benign	Het
Dnah7b	T	A	1: 46,307,830 (GRCm39)	M3048K	probably damaging	Het
Dsel	T	C	1: 111,787,187 (GRCm39)	N1116S	probably benign	Het
Efnb1	A	G	X: 98,191,123 (GRCm39)	Y343C	probably damaging	Het
Esco1	A	G	18: 10,574,873 (GRCm39)		probably null	Het
Fancd2	A	G	6: 113,526,282 (GRCm39)	N335S	probably benign	Het
Flnc	A	G	6: 29,448,674 (GRCm39)	Y1304C	probably damaging	Het
Fubp3	A	G	2: 31,490,569 (GRCm39)		probably benign	Het
Gemin4	G	C	11: 76,101,876 (GRCm39)	P962A	probably damaging	Het
Gjc3	A	C	5: 137,955,808 (GRCm39)	L159R	probably damaging	Het
Gli1	A	G	10: 127,172,596 (GRCm39)	L182P	probably damaging	Het
Gm21957	G	T	7: 124,818,625 (GRCm39)		noncoding transcript	Het
Gm28040	AGTG	AGTGGCACCTTTGGTG	1: 133,255,059 (GRCm39)		probably benign	Het
Gm5885	A	G	6: 133,506,238 (GRCm39)		noncoding transcript	Het
Gon4l	A	T	3: 88,794,902 (GRCm39)	T402S	possibly damaging	Het
Gprasp1	G	A	X: 134,702,791 (GRCm39)	E995K	possibly damaging	Het
Gvin-ps5	T	A	7: 105,929,163 (GRCm39)	T245S	probably damaging	Het
Helb	A	T	10: 119,941,926 (GRCm39)	M254K	possibly damaging	Het
Ift20	G	A	11: 78,430,860 (GRCm39)	E68K	probably damaging	Het
Igfn1	AGGG	AGG	1: 135,902,590 (GRCm39)		probably benign	Het
Impdh2	A	G	9: 108,442,546 (GRCm39)	E305G	possibly damaging	Het
Ints7	T	A	1: 191,336,972 (GRCm39)	C351S	possibly damaging	Het
Ipo9	ATCCTCCTCCTCCTCCTC	ATCCTCCTCCTCCTCCTCCTC	1: 135,314,006 (GRCm39)		probably benign	Het
Ipo9	A	G	1: 135,329,988 (GRCm39)	V484A	probably benign	Het
Kcnq3	T	C	15: 65,867,700 (GRCm39)	M648V	probably benign	Het
Kctd16	A	G	18: 40,392,231 (GRCm39)	E273G	possibly damaging	Het
Kif18a	T	A	2: 109,163,848 (GRCm39)	N732K	probably benign	Het
Kif21b	C	T	1: 136,080,002 (GRCm39)	R513W	probably damaging	Het
Klhl36	T	C	8: 120,603,511 (GRCm39)	C589R	possibly damaging	Het
Lck	A	G	4: 129,442,713 (GRCm39)	Y481H	probably damaging	Het
Lmtk2	A	G	5: 144,084,433 (GRCm39)	Y156C	probably damaging	Het
Mical3	A	T	6: 121,008,095 (GRCm39)		probably null	Het
Mki67	A	G	7: 135,297,321 (GRCm39)	I2571T	possibly damaging	Het
Mx2	G	A	16: 97,339,903 (GRCm39)	E20K	probably benign	Het
Myo10	A	G	15: 25,714,194 (GRCm39)	E87G	probably benign	Het
Myo18b	T	C	5: 113,021,892 (GRCm39)	K500R	probably benign	Het
N4bp2l2	A	T	5: 150,571,001 (GRCm39)	I458N	probably damaging	Het
Nat10	T	C	2: 103,561,648 (GRCm39)		probably null	Het
Nipa1	C	A	7: 55,647,259 (GRCm39)		probably null	Het
Nkpd1	A	G	7: 19,258,162 (GRCm39)	Q647R	probably damaging	Het
Nlrc4	T	A	17: 74,754,946 (GRCm39)		probably benign	Het
Nmbr	C	A	10: 14,646,186 (GRCm39)	Y353*	probably null	Het
Nos1ap	T	A	1: 170,156,735 (GRCm39)	D241V	probably damaging	Het
Obsl1	G	A	1: 75,486,756 (GRCm38)	T1764M	probably benign	Het
Optc	A	T	1: 133,831,534 (GRCm39)		probably null	Het
Or4f4b	A	T	2: 111,313,975 (GRCm39)	T95S	probably benign	Het
Or5d43	A	G	2: 88,105,354 (GRCm39)	V13A	probably benign	Het
Or5m13	A	G	2: 85,749,171 (GRCm39)	I301V	probably null	Het
Or6c201	A	G	10: 128,968,875 (GRCm39)	I254T	probably benign	Het
Pank1	G	A	19: 34,856,380 (GRCm39)	R33C	possibly damaging	Het
Pcmtd1	C	T	1: 7,239,789 (GRCm39)	R77C	probably damaging	Het
Phkg2	G	A	7: 127,181,386 (GRCm39)		probably null	Het
Plcb4	G	A	2: 135,818,019 (GRCm39)	V762M	probably damaging	Het
Pramel7	T	A	2: 87,320,321 (GRCm39)	H324L	probably damaging	Het
Prelp	C	T	1: 133,842,869 (GRCm39)	R92K	probably benign	Het
Ptgr3	T	C	18: 84,112,668 (GRCm39)	Y115H	probably benign	Het
Rai1	C	A	11: 60,080,293 (GRCm39)	S1452R	possibly damaging	Het
Ren1	C	G	1: 133,278,516 (GRCm39)		probably null	Het
Rev3l	T	C	10: 39,724,045 (GRCm39)	V785A	probably benign	Het
Ro60	T	C	1: 143,635,772 (GRCm39)	D458G	probably benign	Het
Rufy2	G	T	10: 62,826,773 (GRCm39)	R104L	probably damaging	Het
Senp6	T	A	9: 80,031,102 (GRCm39)	N8K	probably damaging	Het
Septin4	A	T	11: 87,474,262 (GRCm39)	Q60L	probably benign	Het
Sipa1l2	G	A	8: 126,218,230 (GRCm39)	P369L	probably benign	Het
Slc43a1	T	C	2: 84,671,305 (GRCm39)	L37P	probably damaging	Het
Slf1	T	A	13: 77,197,338 (GRCm39)		probably null	Het
Ssr2	T	A	3: 88,483,949 (GRCm39)		probably benign	Het
Syt2	ACTCTCTCT	ACTCTCTCTCT	1: 134,674,479 (GRCm39)		probably benign	Het
Tas2r115	T	C	6: 132,714,321 (GRCm39)	K210R	probably benign	Het
Tbx18	A	G	9: 87,597,706 (GRCm39)	V276A	probably damaging	Het
Tesl1	T	A	X: 23,773,549 (GRCm39)	V350E	probably benign	Het
Tie1	G	A	4: 118,330,008 (GRCm39)	R1072*	probably null	Het
Tm2d2	C	A	8: 25,512,674 (GRCm39)	T174K	probably damaging	Het
Tmem98	A	G	11: 80,705,158 (GRCm39)	D82G	possibly damaging	Het
Tmub2	A	G	11: 102,178,379 (GRCm39)	E94G	possibly damaging	Het
Tnnt2	TG	TGG	1: 135,774,499 (GRCm39)		probably benign	Het
Tonsl	A	T	15: 76,516,861 (GRCm39)	I923N	probably damaging	Het
Ttc21a	G	A	9: 119,793,361 (GRCm39)	V977M	probably damaging	Het
Ube2ql1	T	C	13: 69,886,783 (GRCm39)	D226G	probably damaging	Het
Ubr4	C	T	4: 139,204,518 (GRCm39)	T4810M	probably damaging	Het
Wdr87-ps	T	C	7: 29,230,935 (GRCm39)		noncoding transcript	Het
Zfp408	A	G	2: 91,478,194 (GRCm39)		probably benign	Het
Zfp473	T	C	7: 44,382,501 (GRCm39)	T610A	possibly damaging	Het
Zfp804b	A	G	5: 6,822,583 (GRCm39)	V160A	probably benign	Het
Zfp960	A	G	17: 17,308,146 (GRCm39)	T287A	probably benign	Het

Other mutations in Nfasc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Nfasc	APN	1	132,501,536 (GRCm39)	nonsense	probably null
IGL01088:Nfasc	APN	1	132,570,514 (GRCm39)	utr 5 prime	probably benign
IGL01958:Nfasc	APN	1	132,536,176 (GRCm39)	nonsense	probably null
IGL01999:Nfasc	APN	1	132,532,985 (GRCm39)	splice site	probably benign
IGL02170:Nfasc	APN	1	132,538,104 (GRCm39)	nonsense	probably null
IGL02187:Nfasc	APN	1	132,498,219 (GRCm39)	missense	probably damaging	1.00
IGL02192:Nfasc	APN	1	132,498,219 (GRCm39)	missense	probably damaging	1.00
IGL02452:Nfasc	APN	1	132,548,662 (GRCm39)	critical splice donor site	probably null
IGL02698:Nfasc	APN	1	132,562,475 (GRCm39)	missense	probably benign	0.06
IGL02797:Nfasc	APN	1	132,538,186 (GRCm39)	missense	probably damaging	1.00
IGL03000:Nfasc	APN	1	132,549,247 (GRCm39)	splice site	probably benign
IGL03027:Nfasc	APN	1	132,538,207 (GRCm39)	missense	probably damaging	1.00
Fascist	UTSW	1	132,539,343 (GRCm39)	missense	probably damaging	1.00
jiggle	UTSW	1	132,529,759 (GRCm39)	missense	probably damaging	1.00
Partisan	UTSW	1	132,533,287 (GRCm39)	missense	probably damaging	1.00
Tremble	UTSW	1	132,539,333 (GRCm39)	missense	probably damaging	1.00
PIT4377001:Nfasc	UTSW	1	132,510,804 (GRCm39)	missense	unknown
R0240:Nfasc	UTSW	1	132,529,721 (GRCm39)	missense	probably damaging	1.00
R0240:Nfasc	UTSW	1	132,529,721 (GRCm39)	missense	probably damaging	1.00
R0241:Nfasc	UTSW	1	132,564,731 (GRCm39)	missense	probably benign	0.02
R0241:Nfasc	UTSW	1	132,564,731 (GRCm39)	missense	probably benign	0.02
R0418:Nfasc	UTSW	1	132,539,333 (GRCm39)	missense	probably damaging	1.00
R0513:Nfasc	UTSW	1	132,531,584 (GRCm39)	missense	possibly damaging	0.95
R0639:Nfasc	UTSW	1	132,531,554 (GRCm39)	missense	probably damaging	1.00
R0646:Nfasc	UTSW	1	132,536,176 (GRCm39)	nonsense	probably null
R1103:Nfasc	UTSW	1	132,534,795 (GRCm39)	splice site	probably benign
R1269:Nfasc	UTSW	1	132,538,526 (GRCm39)	missense	probably damaging	1.00
R1550:Nfasc	UTSW	1	132,536,241 (GRCm39)	missense	probably damaging	0.96
R1749:Nfasc	UTSW	1	132,539,370 (GRCm39)	missense	probably damaging	1.00
R1773:Nfasc	UTSW	1	132,538,577 (GRCm39)	missense	probably damaging	1.00
R1921:Nfasc	UTSW	1	132,538,543 (GRCm39)	missense	probably damaging	1.00
R1987:Nfasc	UTSW	1	132,538,624 (GRCm39)	missense	probably damaging	1.00
R2239:Nfasc	UTSW	1	132,510,760 (GRCm39)	intron	probably benign
R2413:Nfasc	UTSW	1	132,523,243 (GRCm39)	missense	probably damaging	1.00
R2428:Nfasc	UTSW	1	132,523,392 (GRCm39)	missense	possibly damaging	0.55
R2472:Nfasc	UTSW	1	132,515,959 (GRCm39)	intron	probably benign
R2517:Nfasc	UTSW	1	132,525,501 (GRCm39)	splice site	probably null
R3850:Nfasc	UTSW	1	132,559,471 (GRCm39)	missense	probably damaging	1.00
R4050:Nfasc	UTSW	1	132,538,043 (GRCm39)	splice site	probably benign
R4061:Nfasc	UTSW	1	132,525,583 (GRCm39)	missense	probably damaging	1.00
R4088:Nfasc	UTSW	1	132,523,329 (GRCm39)	missense	probably damaging	1.00
R4342:Nfasc	UTSW	1	132,559,443 (GRCm39)	missense	probably damaging	1.00
R4343:Nfasc	UTSW	1	132,559,443 (GRCm39)	missense	probably damaging	1.00
R4345:Nfasc	UTSW	1	132,559,443 (GRCm39)	missense	probably damaging	1.00
R4452:Nfasc	UTSW	1	132,562,409 (GRCm39)	missense	probably damaging	1.00
R4818:Nfasc	UTSW	1	132,531,568 (GRCm39)	missense	possibly damaging	0.87
R4851:Nfasc	UTSW	1	132,529,759 (GRCm39)	missense	probably damaging	1.00
R5014:Nfasc	UTSW	1	132,512,185 (GRCm39)	intron	probably benign
R5768:Nfasc	UTSW	1	132,532,883 (GRCm39)	missense	probably benign	0.00
R6145:Nfasc	UTSW	1	132,562,455 (GRCm39)	missense	probably damaging	1.00
R6335:Nfasc	UTSW	1	132,504,132 (GRCm39)	missense	probably damaging	0.98
R6379:Nfasc	UTSW	1	132,498,280 (GRCm39)	nonsense	probably null
R6486:Nfasc	UTSW	1	132,532,952 (GRCm39)	missense	probably damaging	1.00
R7022:Nfasc	UTSW	1	132,548,787 (GRCm39)	missense	probably damaging	1.00
R7062:Nfasc	UTSW	1	132,529,707 (GRCm39)	critical splice donor site	probably null
R7084:Nfasc	UTSW	1	132,498,247 (GRCm39)	missense	unknown
R7275:Nfasc	UTSW	1	132,562,001 (GRCm39)	missense	probably damaging	1.00
R7286:Nfasc	UTSW	1	132,529,790 (GRCm39)	missense	probably damaging	1.00
R7682:Nfasc	UTSW	1	132,501,511 (GRCm39)	missense	unknown
R7838:Nfasc	UTSW	1	132,533,287 (GRCm39)	missense	probably damaging	1.00
R7871:Nfasc	UTSW	1	132,527,751 (GRCm39)	missense	not run
R7938:Nfasc	UTSW	1	132,533,269 (GRCm39)	missense	probably damaging	1.00
R8083:Nfasc	UTSW	1	132,524,320 (GRCm39)	missense	probably benign	0.00
R8482:Nfasc	UTSW	1	132,532,827 (GRCm39)	missense	probably damaging	1.00
R9027:Nfasc	UTSW	1	132,539,343 (GRCm39)	missense	probably damaging	1.00
R9164:Nfasc	UTSW	1	132,562,544 (GRCm39)	missense	probably damaging	1.00
R9488:Nfasc	UTSW	1	132,527,866 (GRCm39)	missense	possibly damaging	0.68
R9651:Nfasc	UTSW	1	132,527,791 (GRCm39)	missense	probably benign	0.04
Z1176:Nfasc	UTSW	1	132,562,376 (GRCm39)	missense	probably benign	0.00
Z1177:Nfasc	UTSW	1	132,559,576 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCACATTTCCTACGGAATGCC -3'
(R):5'- TGAAAGTGGTTGCCAGGCAG -3'

Sequencing Primer
(F):5'- AATGCCCTCTGCGCCCATAG -3'
(R):5'- CCTAAGGTCATGCCTTCA -3'

Posted On

2014-10-01