Mutagenetix > Incidental Mutations

Incidental Mutation 'R2141:Olfr770'

236276

Institutional Source

Beutler Lab

Gene Symbol

Olfr770

Ensembl Gene

ENSMUSG00000047868

Gene Name

olfactory receptor 770

Synonyms

MOR114-5, GA_x6K02T2PULF-10819232-10818297

MMRRC Submission

040144-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R2141 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

129132578-129138998 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 129133006 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 254 (I254T)

Ref Sequence

ENSEMBL: ENSMUSP00000144960 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062314] [ENSMUST00000203887] [ENSMUST00000204250] [ENSMUST00000204712]

Predicted Effect

probably benign
Transcript: ENSMUST00000062314
AA Change: I254T

PolyPhen 2 Score 0.230 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000055193
Gene: ENSMUSG00000047868
AA Change: I254T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	306	1.2e-43	PFAM
Pfam:7tm_1	38	286	5.5e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203887
AA Change: I254T

PolyPhen 2 Score 0.230 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000144950
Gene: ENSMUSG00000047868
AA Change: I254T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	107	1.5e-8	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000204250
AA Change: I254T

PolyPhen 2 Score 0.230 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000144892
Gene: ENSMUSG00000047868
AA Change: I254T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	306	1.2e-43	PFAM
Pfam:7tm_1	38	286	5.5e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000204712
AA Change: I254T

PolyPhen 2 Score 0.230 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000144960
Gene: ENSMUSG00000047868
AA Change: I254T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	130	1.4e-17	PFAM
Pfam:7TM_GPCR_Srsx	32	130	2.4e-4	PFAM
Pfam:7tm_1	38	130	8.8e-16	PFAM

Meta Mutation Damage Score

0.3097

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

97% (107/110)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810065E05Rik	G	T	11: 58,423,926	C150F	probably damaging	Het
4932431P20Rik	T	C	7: 29,531,510		noncoding transcript	Het
Aagab	T	A	9: 63,616,675		probably null	Het
Abca15	A	C	7: 120,407,474	T1654P	probably damaging	Het
Abca17	T	A	17: 24,334,266	Y157F	probably benign	Het
Aftph	A	T	11: 20,698,318	L813*	probably null	Het
Agap1	T	C	1: 89,837,755	I615T	probably damaging	Het
Ak3	T	G	19: 29,022,847	Q221H	probably benign	Het
Aldoa	A	G	7: 126,797,642		probably null	Het
Anxa8	T	C	14: 34,091,916		probably null	Het
Baz2a	A	G	10: 128,123,612	D1329G	probably damaging	Het
Capn9	G	A	8: 124,605,711	G430R	possibly damaging	Het
Cd55	C	T	1: 130,449,423	V333I	probably benign	Het
Cep70	A	G	9: 99,296,385	Y512C	probably damaging	Het
Cfhr2	T	G	1: 139,831,155	R52S	probably benign	Het
Clcn6	A	G	4: 148,024,137	F145S	possibly damaging	Het
Cnksr1	A	G	4: 134,229,628	Y488H	probably damaging	Het
Diablo	A	G	5: 123,523,361	V24A	probably benign	Het
Dnah7b	T	A	1: 46,268,670	M3048K	probably damaging	Het
Dsel	T	C	1: 111,859,457	N1116S	probably benign	Het
Efnb1	A	G	X: 99,147,517	Y343C	probably damaging	Het
Esco1	A	G	18: 10,574,873		probably null	Het
Fam173b	T	C	15: 31,609,572	F146L	probably benign	Het
Fam26f	G	T	10: 34,127,695	A72E	probably damaging	Het
Fancd2	A	G	6: 113,549,321	N335S	probably benign	Het
Flnc	A	G	6: 29,448,675	Y1304C	probably damaging	Het
Fubp3	A	G	2: 31,600,557		probably benign	Het
Gemin4	G	C	11: 76,211,050	P962A	probably damaging	Het
Gjc3	A	C	5: 137,957,546	L159R	probably damaging	Het
Gli1	A	G	10: 127,336,727	L182P	probably damaging	Het
Gm21957	G	T	7: 125,219,453		noncoding transcript	Het
Gm28040	AGTG	AGTGGCACCTTTGGTG	1: 133,327,321		probably benign	Het
Gm4907	T	A	X: 23,907,310	V350E	probably benign	Het
Gm5885	A	G	6: 133,529,275		noncoding transcript	Het
Gm8989	T	A	7: 106,329,956	T245S	probably damaging	Het
Gon4l	A	T	3: 88,887,595	T402S	possibly damaging	Het
Gprasp1	G	A	X: 135,802,042	E995K	possibly damaging	Het
Helb	A	T	10: 120,106,021	M254K	possibly damaging	Het
Ift20	G	A	11: 78,540,034	E68K	probably damaging	Het
Igfn1	AGGG	AGG	1: 135,974,852		probably benign	Het
Impdh2	A	G	9: 108,565,347	E305G	possibly damaging	Het
Ints7	T	A	1: 191,604,860	C351S	possibly damaging	Het
Ipo9	ATCCTCCTCCTCCTCCTC	ATCCTCCTCCTCCTCCTCCTC	1: 135,386,268		probably benign	Het
Ipo9	A	G	1: 135,402,250	V484A	probably benign	Het
Kcnq3	T	C	15: 65,995,851	M648V	probably benign	Het
Kctd16	A	G	18: 40,259,178	E273G	possibly damaging	Het
Kif18a	T	A	2: 109,333,503	N732K	probably benign	Het
Kif21b	C	T	1: 136,152,264	R513W	probably damaging	Het
Klhl36	T	C	8: 119,876,772	C589R	possibly damaging	Het
Lck	A	G	4: 129,548,920	Y481H	probably damaging	Het
Lmtk2	A	G	5: 144,147,615	Y156C	probably damaging	Het
Mical3	A	T	6: 121,031,134		probably null	Het
Mki67	A	G	7: 135,695,592	I2571T	possibly damaging	Het
Mx2	G	A	16: 97,538,703	E20K	probably benign	Het
Myo10	A	G	15: 25,714,108	E87G	probably benign	Het
Myo18b	T	C	5: 112,874,026	K500R	probably benign	Het
N4bp2l2	A	T	5: 150,647,536	I458N	probably damaging	Het
Nat10	T	C	2: 103,731,303		probably null	Het
Nfasc	G	A	1: 132,596,645	P932L	probably damaging	Het
Nipa1	C	A	7: 55,997,511		probably null	Het
Nkpd1	A	G	7: 19,524,237	Q647R	probably damaging	Het
Nlrc4	T	A	17: 74,447,951		probably benign	Het
Nmbr	C	A	10: 14,770,442	Y353*	probably null	Het
Nos1ap	T	A	1: 170,329,166	D241V	probably damaging	Het
Obsl1	G	A	1: 75,486,756	T1764M	probably benign	Het
Olfr1025-ps1	A	G	2: 85,918,827	I301V	probably null	Het
Olfr1173	A	G	2: 88,275,010	V13A	probably benign	Het
Olfr1289	A	T	2: 111,483,630	T95S	probably benign	Het
Optc	A	T	1: 133,903,796		probably null	Het
Pank1	G	A	19: 34,878,980	R33C	possibly damaging	Het
Pcmtd1	C	T	1: 7,169,565	R77C	probably damaging	Het
Phkg2	G	A	7: 127,582,214		probably null	Het
Plcb4	G	A	2: 135,976,099	V762M	probably damaging	Het
Pramel7	T	A	2: 87,489,977	H324L	probably damaging	Het
Prelp	C	T	1: 133,915,131	R92K	probably benign	Het
Rai1	C	A	11: 60,189,467	S1452R	possibly damaging	Het
Ren1	C	G	1: 133,350,778		probably null	Het
Rev3l	T	C	10: 39,848,049	V785A	probably benign	Het
Rufy2	G	T	10: 62,990,994	R104L	probably damaging	Het
Senp6	T	A	9: 80,123,820	N8K	probably damaging	Het
Sept4	A	T	11: 87,583,436	Q60L	probably benign	Het
Sipa1l2	G	A	8: 125,491,491	P369L	probably benign	Het
Slc43a1	T	C	2: 84,840,961	L37P	probably damaging	Het
Slf1	T	A	13: 77,049,219		probably null	Het
Ssr2	T	A	3: 88,576,642		probably benign	Het
Syt2	ACTCTCTCT	ACTCTCTCTCT	1: 134,746,741		probably benign	Het
Tas2r115	T	C	6: 132,737,358	K210R	probably benign	Het
Tbx18	A	G	9: 87,715,653	V276A	probably damaging	Het
Tie1	G	A	4: 118,472,811	R1072*	probably null	Het
Tm2d2	C	A	8: 25,022,658	T174K	probably damaging	Het
Tmem98	A	G	11: 80,814,332	D82G	possibly damaging	Het
Tmub2	A	G	11: 102,287,553	E94G	possibly damaging	Het
Tnnt2	TG	TGG	1: 135,846,761		probably benign	Het
Tonsl	A	T	15: 76,632,661	I923N	probably damaging	Het
Trove2	T	C	1: 143,760,034	D458G	probably benign	Het
Ttc21a	G	A	9: 119,964,295	V977M	probably damaging	Het
Ube2ql1	T	C	13: 69,738,664	D226G	probably damaging	Het
Ubr4	C	T	4: 139,477,207	T4810M	probably damaging	Het
Zadh2	T	C	18: 84,094,543	Y115H	probably benign	Het
Zfp408	A	G	2: 91,647,849		probably benign	Het
Zfp473	T	C	7: 44,733,077	T610A	possibly damaging	Het
Zfp804b	A	G	5: 6,772,583	V160A	probably benign	Het
Zfp960	A	G	17: 17,087,884	T287A	probably benign	Het

Other mutations in Olfr770

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0787:Olfr770	UTSW	10	129133526	missense	possibly damaging	0.89
R4190:Olfr770	UTSW	10	129132967	missense	possibly damaging	0.86
R4397:Olfr770	UTSW	10	129133581	missense	possibly damaging	0.59
R5009:Olfr770	UTSW	10	129133615	missense	probably benign	0.06
R5781:Olfr770	UTSW	10	129133147	missense	probably damaging	1.00
R6343:Olfr770	UTSW	10	129133666	nonsense	probably null
R7492:Olfr770	UTSW	10	129133640	missense	probably damaging	0.99
R7530:Olfr770	UTSW	10	129133980	splice site	probably null
Z1088:Olfr770	UTSW	10	129133075	missense	probably benign	0.36

Predicted Primers

PCR Primer
(F):5'- TAGAGAAGGCTTGGACCACC -3'
(R):5'- GCAGATGGTTATAGGATGTGCC -3'

Sequencing Primer
(F):5'- GAAATCTTGAATCATCCATTTTGGC -3'
(R):5'- ATAGGATGTGCCGTGCTGACC -3'

Posted On

2014-10-01