Mutagenetix > Incidental Mutation

Incidental Mutation 'R2144:Wnk1'

236614

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

6430573H23Rik, Hsn2, EG406236, Prkwnk1

MMRRC Submission

040147-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2144 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

119923969-120038672 bp(-) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 119948988 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000177761] [ENSMUST00000203030]

AlphaFold

P83741

Predicted Effect

unknown
Transcript: ENSMUST00000060043
AA Change: S1421P

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: S1421P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000088644
AA Change: S1679P

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: S1679P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000088646
AA Change: S1267P

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: S1267P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159397

Predicted Effect

probably benign
Transcript: ENSMUST00000159785

SMART Domains

Protein: ENSMUSP00000124905
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	159	169	N/A	INTRINSIC
low complexity region	183	199	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160769

Predicted Effect

probably benign
Transcript: ENSMUST00000161048

SMART Domains

Protein: ENSMUSP00000125345
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
Blast:S_TKc	2	68	3e-34	BLAST
SCOP:d1phk__	3	70	2e-7	SMART
Pfam:OSR1_C	89	126	9.5e-21	PFAM
coiled coil region	151	185	N/A	INTRINSIC
low complexity region	323	335	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161185

Predicted Effect

probably benign
Transcript: ENSMUST00000161802

SMART Domains

Protein: ENSMUSP00000124724
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	24	50	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162368

SMART Domains

Protein: ENSMUSP00000125383
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	83	95	N/A	INTRINSIC
low complexity region	142	158	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162541

SMART Domains

Protein: ENSMUSP00000125193
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	63	84	N/A	INTRINSIC
low complexity region	91	105	N/A	INTRINSIC
low complexity region	131	157	N/A	INTRINSIC
low complexity region	247	265	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000177761
AA Change: S1670P

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: S1670P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000203030
AA Change: S1172P

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: S1172P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204207

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930553M12Rik	G	A	4: 88,868,174	T69I	unknown	Het
4930553M12Rik	T	A	4: 88,868,175	T69S	unknown	Het
Acsl6	A	T	11: 54,341,778	Q485L	probably damaging	Het
Adam5	C	A	8: 24,815,480	V81F	probably benign	Het
Armc4	C	T	18: 7,127,229	E995K	probably damaging	Het
Atp5s	A	G	12: 69,741,054	Q88R	probably damaging	Het
Bag2	A	G	1: 33,746,831	S137P	possibly damaging	Het
Birc6	A	T	17: 74,660,413	Q4103L	possibly damaging	Het
Camta2	A	G	11: 70,671,575	F999L	probably benign	Het
Cap2	A	T	13: 46,560,502		probably null	Het
Ccnk	T	A	12: 108,189,090	L102Q	probably null	Het
Cd52	T	C	4: 134,093,737		probably benign	Het
Cdc123	A	T	2: 5,810,806	I160K	probably benign	Het
Cep85l	T	C	10: 53,358,126	N52S	probably benign	Het
Cntnap5a	C	T	1: 116,101,710	T298I	probably benign	Het
Cpsf4	G	T	5: 145,178,762	S192I	probably benign	Het
Cpxm1	A	G	2: 130,397,410	S33P	probably benign	Het
Cyp2a12	A	T	7: 27,034,769	T376S	possibly damaging	Het
Cyp3a16	A	G	5: 145,456,084	F137S	probably damaging	Het
Des	A	G	1: 75,366,804	T444A	probably benign	Het
Dgcr8	C	T	16: 18,284,256	G54D	probably damaging	Het
Doxl2	A	G	6: 48,975,291	H50R	probably benign	Het
Dsc3	A	G	18: 19,980,686	F393S	possibly damaging	Het
Dstyk	T	A	1: 132,463,375	M838K	probably damaging	Het
Dvl1	G	A	4: 155,847,816	V28I	possibly damaging	Het
Eml5	A	C	12: 98,810,605	F1417C	probably damaging	Het
Ep400	A	T	5: 110,703,518	M1366K	unknown	Het
Epg5	G	T	18: 77,954,197	C425F	possibly damaging	Het
Epha3	C	G	16: 63,773,317	R136P	possibly damaging	Het
Extl1	C	A	4: 134,371,044	E225D	probably benign	Het
Fam186b	A	G	15: 99,280,657	Y263H	probably benign	Het
Fbn2	A	G	18: 58,052,993	V1761A	possibly damaging	Het
Fer1l6	T	A	15: 58,627,534	M1251K	probably benign	Het
Gart	A	T	16: 91,630,081	I555N	probably damaging	Het
Gm11596	A	T	11: 99,792,963	C110*	probably null	Het
Gnptab	C	T	10: 88,428,506	S262L	possibly damaging	Het
Gpr21	T	C	2: 37,518,231	V263A	probably benign	Het
Gxylt1	T	C	15: 93,254,480	I224V	probably benign	Het
H2-Aa	A	G	17: 34,283,827	S122P	probably damaging	Het
Hsph1	A	C	5: 149,630,337		probably null	Het
Hunk	G	A	16: 90,432,532	D94N	probably damaging	Het
Ikbke	C	A	1: 131,273,474	V176L	probably damaging	Het
Inpp5k	A	T	11: 75,647,191		probably null	Het
Ints10	A	G	8: 68,796,805	T96A	probably damaging	Het
Kansl2	A	T	15: 98,526,631	V306E	probably benign	Het
Kif20a	A	G	18: 34,625,604	D42G	possibly damaging	Het
Klhl7	A	T	5: 24,100,863	M37L	probably benign	Het
Krtap1-5	T	C	11: 99,580,818	I50V	probably benign	Het
Ktn1	A	G	14: 47,714,652	E983G	probably damaging	Het
Lrrk1	G	A	7: 66,296,163	S566L	probably damaging	Het
M6pr	A	G	6: 122,315,367	M174V	probably benign	Het
Man2a2	A	G	7: 80,363,516	S510P	probably damaging	Het
Mmrn1	G	A	6: 60,945,075	S172N	possibly damaging	Het
Mpv17	A	G	5: 31,154,189		probably null	Het
Mrgpra9	T	C	7: 47,235,463	E152G	probably benign	Het
Mst1r	T	C	9: 107,913,168	V660A	probably benign	Het
Myof	A	G	19: 37,981,221		probably null	Het
Myrf	G	A	19: 10,228,674	P126L	probably benign	Het
Nckap1l	C	T	15: 103,475,676	A567V	probably damaging	Het
Nphs1	A	G	7: 30,460,970	E169G	probably benign	Het
Npy1r	T	A	8: 66,705,184	V382D	probably benign	Het
Nrl	A	T	14: 55,520,850	M140K	possibly damaging	Het
Olfr1305	A	G	2: 111,873,423	I144T	probably damaging	Het
Olfr199	C	T	16: 59,216,026	V196M	probably benign	Het
Olfr292	T	A	7: 86,695,280	F275I	probably damaging	Het
Olfr694	A	T	7: 106,688,957	M258K	probably damaging	Het
Olfr924	C	T	9: 38,848,339	T75I	probably damaging	Het
Orc5	T	A	5: 22,547,927	L36F	possibly damaging	Het
Osbpl1a	A	T	18: 12,871,173	S396T	probably benign	Het
Pappa	T	A	4: 65,180,949	Y568*	probably null	Het
Pask	C	T	1: 93,321,297	A794T	probably benign	Het
Pclo	C	T	5: 14,858,752	L5025F	unknown	Het
Pde3a	T	C	6: 141,490,111	V924A	probably benign	Het
Pdpr	A	G	8: 111,118,036	N355S	probably damaging	Het
Pepd	A	T	7: 34,921,418	K36M	probably benign	Het
Pet100	T	G	8: 3,622,355	L14R	probably damaging	Het
Pfkfb2	T	C	1: 130,698,723	T438A	probably benign	Het
Pik3r6	T	A	11: 68,543,611	L546*	probably null	Het
Pira2	A	T	7: 3,844,345	L115Q	probably damaging	Het
Plxdc1	T	C	11: 97,934,012	Y339C	probably damaging	Het
Primpol	A	T	8: 46,586,343	M414K	probably damaging	Het
Prol1	A	T	5: 88,328,395	T215S	unknown	Het
Prss22	A	G	17: 23,994,682	Y212H	probably damaging	Het
Ralgapa2	C	A	2: 146,388,604	V1014L	probably damaging	Het
Rap1gap2	G	A	11: 74,425,976	T245M	probably damaging	Het
Rbm26	T	A	14: 105,115,202	R1009*	probably null	Het
Rbm42	A	G	7: 30,641,110	*450Q	probably null	Het
Rere	T	C	4: 150,616,931	V1256A	probably damaging	Het
Rmi1	G	T	13: 58,407,983	L15F	probably damaging	Het
Rnf213	T	C	11: 119,443,690	S3242P	probably damaging	Het
Rtel1	T	A	2: 181,323,706	V167E	probably damaging	Het
Scgb1b2	G	T	7: 31,291,763		probably benign	Het
Sin3b	T	C	8: 72,731,265	L203P	probably damaging	Het
Skint6	T	C	4: 113,236,260	S229G	possibly damaging	Het
Slco1a4	T	C	6: 141,809,378	Y566C	probably damaging	Het
Smgc	T	G	15: 91,844,421	D121E	possibly damaging	Het
Sned1	T	A	1: 93,271,684	F495L	probably damaging	Het
St7	A	T	6: 17,886,007	N52I	possibly damaging	Het
Sycn	C	A	7: 28,541,069	Q54K	probably benign	Het
Syngr4	A	G	7: 45,887,040	V186A	probably benign	Het
Tarsl2	G	A	7: 65,655,791	M254I	possibly damaging	Het
Tcaf2	A	T	6: 42,642,804	H96Q	probably benign	Het
Tcp11l2	T	C	10: 84,613,499	Y443H	probably damaging	Het
Tmem200c	A	T	17: 68,842,249	Q609L	possibly damaging	Het
Tmx3	G	A	18: 90,517,490	G83R	probably damaging	Het
Tpgs2	A	C	18: 25,168,541	V23G	possibly damaging	Het
Trhr	T	C	15: 44,197,183	V33A	probably benign	Het
Trim66	A	T	7: 109,475,113	I647N	probably damaging	Het
Trnt1	A	G	6: 106,778,039	K244E	probably damaging	Het
Tsfm	G	A	10: 127,028,445	Q134*	probably null	Het
Ttll9	A	G	2: 153,003,007	T432A	probably benign	Het
Vmn2r78	C	T	7: 86,954,482	L623F	probably damaging	Het
Wdr55	A	G	18: 36,762,366	N132S	possibly damaging	Het
Wipf2	A	T	11: 98,896,214	R356S	possibly damaging	Het
Zfp260	A	G	7: 30,105,340	K222E	probably damaging	Het
Zfp300	A	G	X: 21,081,951	S525P	possibly damaging	Het
Zfp592	A	G	7: 81,038,202	T959A	probably benign	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119960708	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119937411	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119948397	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119963485	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119949078	unclassified	probably benign
IGL02268:Wnk1	APN	6	119937373	nonsense	probably null
IGL02348:Wnk1	APN	6	119963328	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119963454	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119937862	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119926633	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119954187	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119953895	missense	probably benign	0.41
Actor	UTSW	6	119970978	missense	probably damaging	1.00
Bad	UTSW	6	119965678	intron	probably benign
Blink	UTSW	6	120002273	nonsense	probably null
Knock	UTSW	6	119943878	splice site	probably null
Narrow	UTSW	6	119992408	missense	probably damaging	1.00
nictitate	UTSW	6	120002280	missense	possibly damaging	0.88
opportunity	UTSW	6	119951068	missense	probably damaging	1.00
path	UTSW	6	120037149	missense	probably damaging	0.99
Stormy	UTSW	6	119992447	missense	probably damaging	1.00
tear	UTSW	6	119963470	missense	probably damaging	0.98
Tic	UTSW	6	119950083	missense	probably damaging	1.00
Unforgiving	UTSW	6	119948282	missense	probably damaging	1.00
Window	UTSW	6	119992453	missense	probably damaging	1.00
Woke	UTSW	6	119962955	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119944799	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119963367	nonsense	probably null
R0044:Wnk1	UTSW	6	120037149	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119952733	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119928163	intron	probably benign
R0453:Wnk1	UTSW	6	119963151	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119969332	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119962803	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119926564	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119951992	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119926683	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119948505	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119952006	splice site	probably benign
R1188:Wnk1	UTSW	6	119948709	nonsense	probably null
R1245:Wnk1	UTSW	6	119948457	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119950684	splice site	probably benign
R1869:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119952923	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119934382	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119937578	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119928157	splice site	probably null
R2186:Wnk1	UTSW	6	119948567	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119963640	splice site	probably null
R2338:Wnk1	UTSW	6	119969534	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119936367	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119992453	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119969354	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119949051	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119951068	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119948126	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119949261	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119953895	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119926426	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119951911	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119965744	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119963470	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119952771	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119962438	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119949377	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119965735	intron	probably benign
R5002:Wnk1	UTSW	6	119937963	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119965735	intron	probably benign
R5152:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120037188	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119968523	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119948891	unclassified	probably benign
R5600:Wnk1	UTSW	6	119949358	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119992408	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120037601	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119972997	intron	probably benign
R6128:Wnk1	UTSW	6	119963786	splice site	probably null
R6237:Wnk1	UTSW	6	119952767	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119948585	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119962955	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119948282	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119948781	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119965678	intron	probably benign
R7024:Wnk1	UTSW	6	119965726	intron	probably benign
R7072:Wnk1	UTSW	6	119937861	missense	unknown
R7087:Wnk1	UTSW	6	120037530	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119948307	missense	unknown
R7134:Wnk1	UTSW	6	119926428	missense	unknown
R7137:Wnk1	UTSW	6	120038212	unclassified	probably benign
R7142:Wnk1	UTSW	6	119949279	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119970978	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119943878	splice site	probably null
R7218:Wnk1	UTSW	6	120002273	nonsense	probably null
R7498:Wnk1	UTSW	6	119927196	missense	unknown
R7599:Wnk1	UTSW	6	119929828	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119932738	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119949176	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120037448	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119932714	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119953833	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119963493	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119929999	splice site	probably null
R8359:Wnk1	UTSW	6	119992447	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119934427	missense	unknown
R8519:Wnk1	UTSW	6	119950083	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120036998	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119962432	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119948670	missense	unknown
R9148:Wnk1	UTSW	6	119948670	missense	unknown
R9354:Wnk1	UTSW	6	119965699	missense	unknown
R9379:Wnk1	UTSW	6	119951717	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120037032	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119948205	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGAAGCCATGCCTGGGAATG -3'
(R):5'- ACATCAGTGATGCAGTCTGAGAC -3'

Sequencing Primer
(F):5'- TAGAGACTGATGTCACTATGGCAACC -3'
(R):5'- AGTCTGAGACTGCACTGCC -3'

Posted On

2014-10-01