Mutagenetix > Incidental Mutation

Incidental Mutation 'R2175:Vmn2r63'

236826

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

040177-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R2175 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

42552675-42583213 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to T at 42583004 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803] [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

probably null
Transcript: ENSMUST00000163803

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000163803

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam34l	T	A	8: 44,078,475 (GRCm39)	Y583F	probably benign	Het
Adra2a	A	G	19: 54,034,793 (GRCm39)	T50A	probably benign	Het
Alox8	T	C	11: 69,078,592 (GRCm39)	Y367C	possibly damaging	Het
Ankfn1	A	G	11: 89,417,363 (GRCm39)	L83P	probably damaging	Het
Asxl3	T	C	18: 22,649,652 (GRCm39)	L547P	probably benign	Het
Cox10	G	A	11: 63,962,475 (GRCm39)	A122V	probably benign	Het
Cttnbp2	A	C	6: 18,434,828 (GRCm39)		probably null	Het
Eml5	A	T	12: 98,842,482 (GRCm39)	C205*	probably null	Het
Ephx3	T	A	17: 32,407,433 (GRCm39)	T204S	possibly damaging	Het
Evi5l	A	G	8: 4,237,269 (GRCm39)	H124R	probably damaging	Het
Ext1	G	T	15: 52,932,124 (GRCm39)	P726Q	probably damaging	Het
Fam151b	T	C	13: 92,614,426 (GRCm39)	R21G	probably damaging	Het
Hdac1	G	A	4: 129,428,463 (GRCm39)	R36C	probably damaging	Het
Ift172	A	G	5: 31,424,029 (GRCm39)	Y715H	probably damaging	Het
Inpp4b	T	C	8: 82,583,328 (GRCm39)	F144S	probably damaging	Het
Lepr	T	A	4: 101,622,576 (GRCm39)	I452N	probably benign	Het
Mok	T	C	12: 110,781,634 (GRCm39)	H6R	probably benign	Het
Myef2	A	T	2: 124,940,375 (GRCm39)	M392K	probably damaging	Het
Ncoa2	G	A	1: 13,294,837 (GRCm39)	P28S	probably damaging	Het
Nfrkb	A	G	9: 31,300,310 (GRCm39)	T34A	possibly damaging	Het
Nkx1-1	G	A	5: 33,588,598 (GRCm39)	A230V	probably benign	Het
Or10ag2	T	A	2: 87,248,500 (GRCm39)	L34H	probably damaging	Het
Or5an1b	T	A	19: 12,299,885 (GRCm39)	Y102F	probably damaging	Het
Ryr1	T	G	7: 28,767,867 (GRCm39)	K2890T	probably damaging	Het
Siae	G	T	9: 37,539,092 (GRCm39)	D168Y	probably damaging	Het
Smarcc1	A	T	9: 109,993,877 (GRCm39)	T241S	possibly damaging	Het
Steap2	T	A	5: 5,723,501 (GRCm39)	I460F	probably damaging	Het
Strap	A	G	6: 137,727,590 (GRCm39)	T345A	probably benign	Het
Tex2	A	G	11: 106,394,513 (GRCm39)	V1099A	unknown	Het
Thsd7a	T	C	6: 12,331,943 (GRCm39)	T1290A	possibly damaging	Het
Unc80	G	A	1: 66,716,514 (GRCm39)	G2878D	probably damaging	Het
Xirp2	C	T	2: 67,340,258 (GRCm39)	T833I	probably damaging	Het
Zcchc2	T	C	1: 105,955,153 (GRCm39)	S615P	probably damaging	Het
Zfp148	C	G	16: 33,317,116 (GRCm39)	S554*	probably null	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42,553,543 (GRCm39)	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42,552,788 (GRCm39)	missense	probably benign
IGL02203:Vmn2r63	APN	7	42,553,432 (GRCm39)	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42,576,274 (GRCm39)	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42,552,878 (GRCm39)	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42,578,616 (GRCm39)	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42,577,368 (GRCm39)	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42,553,409 (GRCm39)	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42,576,514 (GRCm39)	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42,552,699 (GRCm39)	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42,553,042 (GRCm39)	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42,583,129 (GRCm39)	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42,577,952 (GRCm39)	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42,577,434 (GRCm39)	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42,577,459 (GRCm39)	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42,577,339 (GRCm39)	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42,578,015 (GRCm39)	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42,553,550 (GRCm39)	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42,577,743 (GRCm39)	missense	probably benign
R1698:Vmn2r63	UTSW	7	42,583,038 (GRCm39)	missense	probably benign
R1753:Vmn2r63	UTSW	7	42,577,669 (GRCm39)	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42,576,297 (GRCm39)	missense	probably damaging	0.99
R2261:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42,552,829 (GRCm39)	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42,577,537 (GRCm39)	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42,583,250 (GRCm39)	splice site	probably null
R4649:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42,576,314 (GRCm39)	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42,553,402 (GRCm39)	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42,553,169 (GRCm39)	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42,577,701 (GRCm39)	missense	probably benign
R5400:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42,583,104 (GRCm39)	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42,578,435 (GRCm39)	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42,578,059 (GRCm39)	splice site	probably null
R6362:Vmn2r63	UTSW	7	42,552,721 (GRCm39)	missense	probably benign
R6706:Vmn2r63	UTSW	7	42,578,001 (GRCm39)	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42,552,695 (GRCm39)	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42,577,959 (GRCm39)	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42,583,014 (GRCm39)	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42,576,391 (GRCm39)	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42,574,693 (GRCm39)	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42,576,466 (GRCm39)	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42,577,553 (GRCm39)	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42,552,955 (GRCm39)	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42,576,441 (GRCm39)	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42,577,919 (GRCm39)	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42,577,874 (GRCm39)	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42,576,413 (GRCm39)	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42,553,361 (GRCm39)	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42,577,983 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- TTTAAGAAAGCTCAGAACTACCAGGTC -3'
(R):5'- CCAATTTTGTATGTGCCTTTACTGG -3'

Sequencing Primer
(F):5'- GCTCAGAACTACCAGGTCATACTTAG -3'
(R):5'- TGCCTTTACTGGTGATAAATGC -3'

Posted On

2014-10-02