Incidental Mutation 'R0173:Prdm9'
ID 23741
Institutional Source Beutler Lab
Gene Symbol Prdm9
Ensembl Gene ENSMUSG00000051977
Gene Name PR domain containing 9
Synonyms Meisetz, repro7, Dsbc1, Rcr1, G1-419-29
MMRRC Submission 038445-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.351) question?
Stock # R0173 (G1)
Quality Score 225
Status Validated
Chromosome 17
Chromosomal Location 15763341-15784616 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 15764297 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tryptophan to Arginine at position 828 (W828R)
Ref Sequence ENSEMBL: ENSMUSP00000131871 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000167994]
AlphaFold Q96EQ9
Predicted Effect noncoding transcript
Transcript: ENSMUST00000128267
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130297
Predicted Effect probably benign
Transcript: ENSMUST00000167994
AA Change: W828R

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000131871
Gene: ENSMUSG00000051977
AA Change: W828R

DomainStartEndE-ValueType
KRAB 30 89 5.54e-8 SMART
Pfam:SSXRD 175 205 1.5e-20 PFAM
SET 248 368 2.56e-2 SMART
ZnF_C2H2 392 415 3.29e-1 SMART
ZnF_C2H2 516 535 4.74e1 SMART
ZnF_C2H2 541 563 9.73e-4 SMART
ZnF_C2H2 569 591 1.3e-4 SMART
ZnF_C2H2 597 619 1.3e-4 SMART
ZnF_C2H2 625 647 4.24e-4 SMART
ZnF_C2H2 653 675 4.24e-4 SMART
ZnF_C2H2 681 703 1.95e-3 SMART
ZnF_C2H2 709 731 5.99e-4 SMART
ZnF_C2H2 737 759 1.95e-3 SMART
ZnF_C2H2 765 787 1.95e-3 SMART
ZnF_C2H2 793 815 1.3e-4 SMART
ZnF_C2H2 821 843 1.6e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000231919
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.0%
  • 20x: 95.1%
Validation Efficiency 96% (64/67)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]
PHENOTYPE: Mice homozygous for a knock-out allele show decreased oocyte number, azoospermia, and sterility in both sexes due to severe impairment of the double-stranded break repair pathway, deficient pairing of homologous chromosomes, and impaired sex body formation. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A330008L17Rik G A 8: 100,148,286 (GRCm39) noncoding transcript Het
Akt1s1 C T 7: 44,502,284 (GRCm39) P95S possibly damaging Het
Ambra1 T C 2: 91,640,564 (GRCm39) probably benign Het
Aunip T A 4: 134,250,861 (GRCm39) W269R probably damaging Het
Bmper A G 9: 23,136,125 (GRCm39) M69V probably benign Het
Cdh2 A T 18: 16,783,314 (GRCm39) probably benign Het
Cenpe T C 3: 134,965,744 (GRCm39) M2074T probably benign Het
Col14a1 C T 15: 55,351,928 (GRCm39) P1592S probably damaging Het
Csgalnact1 G A 8: 68,913,681 (GRCm39) R175C probably damaging Het
Dtx1 A G 5: 120,820,818 (GRCm39) probably benign Het
Elmod3 T C 6: 72,554,571 (GRCm39) D154G probably damaging Het
Eno1b T C 18: 48,180,806 (GRCm39) I328T probably benign Het
Gab1 T C 8: 81,526,789 (GRCm39) D103G possibly damaging Het
Gon4l A G 3: 88,765,710 (GRCm39) D377G probably damaging Het
Gramd1c C T 16: 43,818,196 (GRCm39) R328K possibly damaging Het
Hdac3 A G 18: 38,074,806 (GRCm39) S312P probably damaging Het
Hmcn2 T C 2: 31,328,343 (GRCm39) probably null Het
Intu T C 3: 40,629,776 (GRCm39) probably null Het
Lnpk T C 2: 74,381,409 (GRCm39) K118R probably damaging Het
Lzts3 A C 2: 130,476,688 (GRCm39) *587G probably null Het
Mctp2 C T 7: 71,896,855 (GRCm39) probably null Het
Miox C T 15: 89,220,477 (GRCm39) L189F possibly damaging Het
Mmp23 G T 4: 155,735,222 (GRCm39) R374S possibly damaging Het
Morc3 G A 16: 93,629,094 (GRCm39) probably null Het
Mymk C T 2: 26,952,262 (GRCm39) A161T probably damaging Het
Ncoa6 TGC TGCGC 2: 155,250,211 (GRCm39) probably null Het
Neb T C 2: 52,133,859 (GRCm39) S3375G probably damaging Het
Nedd1 T C 10: 92,534,745 (GRCm39) D255G probably benign Het
Nid2 T C 14: 19,852,400 (GRCm39) probably benign Het
Nr1d2 A G 14: 18,215,502 (GRCm38) probably benign Het
Nus1 A G 10: 52,294,094 (GRCm39) H86R possibly damaging Het
Or5b118 A T 19: 13,449,065 (GRCm39) I244F probably benign Het
Or7g17 A G 9: 18,768,325 (GRCm39) I135V probably damaging Het
Plcxd2 A T 16: 45,785,542 (GRCm39) probably null Het
Prkd2 T C 7: 16,582,969 (GRCm39) S244P probably benign Het
Psmd4 A T 3: 94,940,234 (GRCm39) L159H probably damaging Het
Qprt C T 7: 126,707,543 (GRCm39) G215E probably damaging Het
Rab3gap2 C A 1: 184,982,104 (GRCm39) H385Q possibly damaging Het
Rapgef5 A G 12: 117,652,411 (GRCm39) D300G probably benign Het
Rbl1 A T 2: 157,001,605 (GRCm39) N894K probably benign Het
Rgma C T 7: 73,067,302 (GRCm39) R280W probably damaging Het
Rims4 C T 2: 163,705,849 (GRCm39) V262M possibly damaging Het
Rundc3a T A 11: 102,289,071 (GRCm39) probably benign Het
Scaf11 A T 15: 96,318,075 (GRCm39) D496E probably benign Het
Scn9a T C 2: 66,363,437 (GRCm39) Y936C probably damaging Het
Sdk1 A G 5: 142,159,564 (GRCm39) probably benign Het
Serpinb9 G A 13: 33,194,705 (GRCm39) D154N probably benign Het
Slc48a1 A T 15: 97,688,555 (GRCm39) H131L possibly damaging Het
Slco1a6 T C 6: 142,048,848 (GRCm39) N311D probably benign Het
Sorl1 A G 9: 41,979,229 (GRCm39) V423A probably damaging Het
Srrm2 C A 17: 24,034,103 (GRCm39) probably benign Het
Srsf12 A T 4: 33,226,117 (GRCm39) S122C probably damaging Het
Suclg2 G C 6: 95,452,154 (GRCm39) probably benign Het
Tbpl1 A T 10: 22,583,523 (GRCm39) L149* probably null Het
Tmem144 G A 3: 79,746,580 (GRCm39) probably benign Het
Tmem63a T C 1: 180,782,363 (GRCm39) probably benign Het
Tut1 C T 19: 8,942,847 (GRCm39) R645* probably null Het
Ubqln4 T A 3: 88,462,686 (GRCm39) D50E probably benign Het
Ubr5 A G 15: 38,004,919 (GRCm39) S1227P probably damaging Het
Vipas39 A G 12: 87,297,285 (GRCm39) probably benign Het
Vmn1r28 G A 6: 58,242,702 (GRCm39) A182T probably benign Het
Vps26b G A 9: 26,924,101 (GRCm39) T214I probably benign Het
Xpc A G 6: 91,481,717 (GRCm39) probably benign Het
Other mutations in Prdm9
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01302:Prdm9 APN 17 15,773,608 (GRCm39) missense probably benign 0.11
IGL02322:Prdm9 APN 17 15,783,110 (GRCm39) missense probably damaging 0.99
IGL02354:Prdm9 APN 17 15,783,109 (GRCm39) missense probably damaging 1.00
IGL02361:Prdm9 APN 17 15,783,109 (GRCm39) missense probably damaging 1.00
IGL02724:Prdm9 APN 17 15,783,522 (GRCm39) missense probably benign 0.07
IGL03120:Prdm9 APN 17 15,765,193 (GRCm39) missense probably benign
berlin UTSW 17 15,782,702 (GRCm39) missense probably damaging 0.96
R0173:Prdm9 UTSW 17 15,764,275 (GRCm39) missense probably benign 0.02
R0309:Prdm9 UTSW 17 15,777,646 (GRCm39) missense probably damaging 0.98
R1420:Prdm9 UTSW 17 15,764,638 (GRCm39) missense probably damaging 1.00
R3498:Prdm9 UTSW 17 15,783,207 (GRCm39) splice site probably benign
R3714:Prdm9 UTSW 17 15,777,623 (GRCm39) nonsense probably null
R4118:Prdm9 UTSW 17 15,764,275 (GRCm39) missense probably benign 0.02
R4369:Prdm9 UTSW 17 15,764,708 (GRCm39) missense probably benign 0.14
R4691:Prdm9 UTSW 17 15,773,640 (GRCm39) missense probably benign 0.03
R4742:Prdm9 UTSW 17 15,773,783 (GRCm39) missense probably damaging 0.99
R4910:Prdm9 UTSW 17 15,764,585 (GRCm39) missense probably benign 0.08
R5056:Prdm9 UTSW 17 15,782,679 (GRCm39) missense possibly damaging 0.93
R5130:Prdm9 UTSW 17 15,764,729 (GRCm39) missense probably benign 0.00
R5175:Prdm9 UTSW 17 15,777,713 (GRCm39) missense probably benign 0.04
R5187:Prdm9 UTSW 17 15,783,155 (GRCm39) missense probably damaging 0.98
R5213:Prdm9 UTSW 17 15,775,416 (GRCm39) missense probably damaging 0.98
R5270:Prdm9 UTSW 17 15,773,625 (GRCm39) missense probably benign 0.16
R5635:Prdm9 UTSW 17 15,782,702 (GRCm39) missense probably damaging 0.96
R6753:Prdm9 UTSW 17 15,765,218 (GRCm39) missense probably benign 0.00
R6857:Prdm9 UTSW 17 15,764,518 (GRCm39) missense probably benign 0.04
R7041:Prdm9 UTSW 17 15,765,257 (GRCm39) missense possibly damaging 0.56
R7355:Prdm9 UTSW 17 15,765,497 (GRCm39) missense probably benign 0.01
R7410:Prdm9 UTSW 17 15,765,259 (GRCm39) missense possibly damaging 0.73
R7570:Prdm9 UTSW 17 15,775,914 (GRCm39) missense probably benign 0.14
R7571:Prdm9 UTSW 17 15,783,526 (GRCm39) missense probably damaging 0.98
R7575:Prdm9 UTSW 17 15,764,890 (GRCm39) missense probably damaging 1.00
R7593:Prdm9 UTSW 17 15,764,867 (GRCm39) missense possibly damaging 0.81
R7664:Prdm9 UTSW 17 15,775,833 (GRCm39) missense probably damaging 0.99
R7755:Prdm9 UTSW 17 15,765,226 (GRCm39) missense probably damaging 1.00
R7817:Prdm9 UTSW 17 15,779,311 (GRCm39) missense probably damaging 1.00
R7875:Prdm9 UTSW 17 15,773,804 (GRCm39) nonsense probably null
R8110:Prdm9 UTSW 17 15,774,960 (GRCm39) missense probably damaging 1.00
R8222:Prdm9 UTSW 17 15,765,035 (GRCm39) missense possibly damaging 0.93
R8405:Prdm9 UTSW 17 15,764,456 (GRCm39) missense probably benign 0.22
R8695:Prdm9 UTSW 17 15,765,019 (GRCm39) missense probably damaging 1.00
R8947:Prdm9 UTSW 17 15,764,270 (GRCm39) missense possibly damaging 0.96
X0021:Prdm9 UTSW 17 15,773,734 (GRCm39) nonsense probably null
Predicted Primers PCR Primer
(F):5'- tgtttTTGAGGAGGGGTTGTTCGTT -3'
(R):5'- GCGAAGTCAAACCTCATCCAGCA -3'

Sequencing Primer
(F):5'- gccagggctatacagagaaac -3'
(R):5'- AACCTCATCCAGCACCAGAG -3'
Posted On 2013-04-16