Mutagenetix > Incidental Mutations

Incidental Mutation 'R2209:Olfr1434'

239297

Institutional Source

Beutler Lab

Gene Symbol

Olfr1434

Ensembl Gene

ENSMUSG00000095640

Gene Name

olfactory receptor 1434

Synonyms

MOR214-4, Olfr1433, MOR214-4, GA_x6K02T2RE5P-2618516-2619454, GA_x6K02T2RE5P-2610001-2610414

MMRRC Submission

040211-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R2209 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

12279854-12285626 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 12283860 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Valine at position 271 (F271V)

Ref Sequence

ENSEMBL: ENSMUSP00000146411 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000207186] [ENSMUST00000207915] [ENSMUST00000208197]

AlphaFold

Q7TQR9

Predicted Effect

probably benign
Transcript: ENSMUST00000087814
AA Change: F271V

PolyPhen 2 Score 0.411 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000085115
Gene: ENSMUSG00000095640
AA Change: F271V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	308	9.2e-55	PFAM
Pfam:7tm_1	42	307	2.5e-18	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000207186
AA Change: F271V

PolyPhen 2 Score 0.411 (Sensitivity: 0.89; Specificity: 0.90)

Predicted Effect

probably benign
Transcript: ENSMUST00000207915

Predicted Effect

probably benign
Transcript: ENSMUST00000208197
AA Change: F271V

PolyPhen 2 Score 0.411 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430548M08Rik	A	G	8: 120,157,488	E330G	possibly damaging	Het
Apob	A	C	12: 8,007,752	D2078A	probably benign	Het
Arhgap21	T	A	2: 20,849,520	Q1681L	probably damaging	Het
Arsb	A	G	13: 93,862,101	T306A	probably benign	Het
Brpf3	G	A	17: 28,828,420	D1053N	probably damaging	Het
Ccdc130	C	T	8: 84,263,869	V45I	probably benign	Het
Cenpf	A	T	1: 189,652,598	I2495N	probably benign	Het
Col12a1	T	C	9: 79,692,352	K840E	possibly damaging	Het
Cry1	A	G	10: 85,146,755	L269P	probably damaging	Het
Cyp21a1	C	A	17: 34,802,727	E289*	probably null	Het
Dcp2	A	T	18: 44,405,514	K215*	probably null	Het
Ecm2	A	G	13: 49,530,156	N537D	probably damaging	Het
Emid1	G	A	11: 5,135,407	T113M	probably benign	Het
Exoc8	C	T	8: 124,896,179	W483*	probably null	Het
Fes	A	T	7: 80,380,283	N582K	probably damaging	Het
Flnb	G	T	14: 7,905,507	E1086*	probably null	Het
Flnc	T	A	6: 29,455,845	D2058E	possibly damaging	Het
Gatb	G	A	3: 85,653,805	D543N	probably benign	Het
Gm14412	A	G	2: 177,317,436	V9A	probably damaging	Het
Gm4884	T	C	7: 41,043,321	V238A	possibly damaging	Het
Igfbp5	A	G	1: 72,873,937	V68A	possibly damaging	Het
Igflr1	T	A	7: 30,567,797	I330N	probably damaging	Het
Il1r2	A	G	1: 40,115,138	T222A	probably benign	Het
Krt87	T	C	15: 101,433,108	E419G	probably benign	Het
Lman2	A	G	13: 55,351,502	S187P	probably damaging	Het
Lrrc6	A	G	15: 66,449,551	I247T	probably benign	Het
Mrpl38	T	C	11: 116,138,462	E76G	possibly damaging	Het
Mtx3	A	G	13: 92,847,604	I130V	probably benign	Het
Naf1	T	G	8: 66,860,536		probably benign	Het
Nkx2-1	T	G	12: 56,533,508	M216L	probably benign	Het
Notch1	C	A	2: 26,460,007	V2374L	probably benign	Het
Nrxn2	A	G	19: 6,493,007	D1087G	probably benign	Het
Nudt8	T	C	19: 4,001,902	F171S	probably damaging	Het
Pds5a	A	T	5: 65,628,014	C916*	probably null	Het
Phyhip	A	T	14: 70,461,894	N46Y	probably damaging	Het
Pomt1	A	G	2: 32,250,862	Y502C	possibly damaging	Het
Prkcg	A	C	7: 3,303,581		probably benign	Het
Prl8a6	C	T	13: 27,435,386	E118K	probably benign	Het
Prpf39	T	C	12: 65,057,915		probably null	Het
Ptprb	A	G	10: 116,369,357	H2159R	probably damaging	Het
Ripor2	A	T	13: 24,701,612	D571V	probably damaging	Het
Rps19	C	T	7: 24,885,127	L34F	probably benign	Het
Rusc1	A	G	3: 89,088,821	S145P	probably damaging	Het
Scn4a	T	A	11: 106,339,225	T586S	probably damaging	Het
Slc7a12	A	G	3: 14,481,064	S90G	possibly damaging	Het
Specc1l	A	G	10: 75,246,576	D619G	probably damaging	Het
Src	A	G	2: 157,462,790	D143G	probably benign	Het
Sst	T	C	16: 23,889,808	N91S	probably benign	Het
Stxbp5l	T	A	16: 37,216,036	I406F	probably damaging	Het
Thsd1	T	A	8: 22,258,871	I525N	probably damaging	Het
Ticam2	A	T	18: 46,560,400	F207I	probably damaging	Het
Tsc22d1	C	A	14: 76,418,740	N31K	probably damaging	Het
Tspan10	T	C	11: 120,446,163	V253A	probably benign	Het
Ttc33	A	G	15: 5,208,443	K99R	possibly damaging	Het
Vmn1r63	T	A	7: 5,803,213	N140I	probably damaging	Het
Zbtb25	A	G	12: 76,349,129	*440Q	probably null	Het
Zfhx4	T	A	3: 5,396,918	C1218S	probably damaging	Het
Zfp84	T	A	7: 29,777,182	I433N	probably damaging	Het

Other mutations in Olfr1434

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01933:Olfr1434	APN	19	12283705	missense	probably damaging	1.00
IGL02415:Olfr1434	APN	19	12283498	missense	probably benign	0.44
IGL02731:Olfr1434	APN	19	12283842	missense	probably damaging	0.99
IGL02803:Olfr1434	APN	19	12283983	missense	possibly damaging	0.94
IGL03050:Olfr1434	UTSW	19	12283512	missense	probably benign
R0432:Olfr1434	UTSW	19	12283903	missense	probably damaging	1.00
R3710:Olfr1434	UTSW	19	12283086	missense	probably damaging	1.00
R4724:Olfr1434	UTSW	19	12283096	missense	probably damaging	1.00
R5133:Olfr1434	UTSW	19	12283306	missense	possibly damaging	0.96
R5974:Olfr1434	UTSW	19	12283836	missense	probably damaging	1.00
R6544:Olfr1434	UTSW	19	12283155	missense	probably damaging	1.00
R7225:Olfr1434	UTSW	19	12283467	missense	probably benign	0.00
R7320:Olfr1434	UTSW	19	12283816	missense	possibly damaging	0.72
R7467:Olfr1434	UTSW	19	12283475	nonsense	probably null
R7900:Olfr1434	UTSW	19	12283341	missense	probably damaging	1.00
R8719:Olfr1434	UTSW	19	12283428	missense	probably benign	0.13
R9135:Olfr1434	UTSW	19	12283444	missense	probably damaging	1.00
R9324:Olfr1434	UTSW	19	12283575	missense	probably benign	0.30

Predicted Primers

PCR Primer
(F):5'- ACGCCTTAGCCATCATGGTG -3'
(R):5'- GCCATGGACTTTGTAGTATTCCAGC -3'

Sequencing Primer
(F):5'- AGCCATCATGGTGTCCTATG -3'
(R):5'- TGTAGTATTCCAGCCATGTGTAC -3'

Posted On

2014-10-15