Mutagenetix > Incidental Mutation

Incidental Mutation 'R2229:Muc5b'

239603

Institutional Source

Beutler Lab

Gene Symbol

Muc5b

Ensembl Gene

ENSMUSG00000066108

Gene Name

mucin 5, subtype B, tracheobronchial

Synonyms

MUC5, MUC9, 2300002I04Rik

MMRRC Submission

040230-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.169) question?

Stock #

R2229 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

141839070-141873084 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 141861644 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 2776 (C2776S)

Ref Sequence

ENSEMBL: ENSMUSP00000128276 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165147]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165147
AA Change: C2776S

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000128276
Gene: ENSMUSG00000066108
AA Change: C2776S

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWD	73	228	1.12e-25	SMART
C8	267	330	7.26e-8	SMART
Pfam:TIL	333	389	1.1e-13	PFAM
VWC	391	459	1.35e-1	SMART
VWD	418	582	2.87e-37	SMART
C8	619	693	2.53e-30	SMART
Pfam:TIL	699	756	2.6e-10	PFAM
VWC	758	823	1.26e0	SMART
VWC	861	930	1.58e-7	SMART
VWD	888	1048	3e-40	SMART
C8	1084	1158	3.75e-33	SMART
low complexity region	1314	1328	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1345	1432	6.7e-27	PFAM
low complexity region	1447	1468	N/A	INTRINSIC
low complexity region	1498	1517	N/A	INTRINSIC
low complexity region	1543	1558	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1574	1663	2.1e-26	PFAM
low complexity region	1678	1693	N/A	INTRINSIC
low complexity region	1728	1745	N/A	INTRINSIC
low complexity region	1778	1831	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1870	1959	2.7e-26	PFAM
low complexity region	1967	1990	N/A	INTRINSIC
low complexity region	2024	2041	N/A	INTRINSIC
low complexity region	2074	2127	N/A	INTRINSIC
Pfam:Mucin2_WxxW	2184	2273	2.1e-26	PFAM
low complexity region	2281	2304	N/A	INTRINSIC
low complexity region	2338	2355	N/A	INTRINSIC
low complexity region	2388	2441	N/A	INTRINSIC
Pfam:Mucin2_WxxW	2498	2587	2.1e-26	PFAM
low complexity region	2596	2618	N/A	INTRINSIC
low complexity region	2623	2654	N/A	INTRINSIC
low complexity region	2660	2681	N/A	INTRINSIC
Pfam:Mucin2_WxxW	2687	2776	3.8e-25	PFAM
low complexity region	2781	2796	N/A	INTRINSIC
low complexity region	2958	3009	N/A	INTRINSIC
Pfam:Mucin2_WxxW	3066	3155	2.6e-26	PFAM
low complexity region	3220	3237	N/A	INTRINSIC
low complexity region	3270	3317	N/A	INTRINSIC
Pfam:Mucin2_WxxW	3380	3469	3.4e-26	PFAM
low complexity region	3509	3529	N/A	INTRINSIC
low complexity region	3546	3562	N/A	INTRINSIC
low complexity region	3568	3591	N/A	INTRINSIC
Pfam:Mucin2_WxxW	3624	3713	2.6e-27	PFAM
Pfam:Mucin2_WxxW	3778	3867	3.2e-24	PFAM
low complexity region	3883	3900	N/A	INTRINSIC
low complexity region	3910	3934	N/A	INTRINSIC
low complexity region	3959	3976	N/A	INTRINSIC
low complexity region	4033	4053	N/A	INTRINSIC
VWD	4111	4283	6.75e-34	SMART
C8	4336	4403	4.26e-14	SMART
VWC	4461	4530	7.06e-5	SMART
VWC	4570	4631	6.53e-9	SMART
CT	4708	4790	2.93e-26	SMART

Meta Mutation Damage Score

0.1776

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

99% (78/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]
PHENOTYPE: Mice homozygous for a knock-out allele accumulate materials in the upper and lower airways leading to chronic infection and inflammation that does not resolve and results in premature death. Macrophage function is impaired. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam24	A	T	8: 40,680,365 (GRCm38)	I291L	probably benign	Het
Adcy8	C	A	15: 64,822,207 (GRCm38)	R407L	possibly damaging	Het
Adgb	A	T	10: 10,436,051 (GRCm38)	V212E	probably damaging	Het
Adgrg7	C	T	16: 56,752,403 (GRCm38)	S350N	probably benign	Het
Agbl1	A	G	7: 76,433,378 (GRCm38)	T448A	probably benign	Het
Aldh1l1	C	G	6: 90,583,186 (GRCm38)	T605R	probably damaging	Het
Arfip1	A	T	3: 84,547,973 (GRCm38)	N18K	probably damaging	Het
Atp6v1b2	C	A	8: 69,102,759 (GRCm38)		probably null	Het
Banp	G	A	8: 121,978,685 (GRCm38)	S98N	probably damaging	Het
Btnl9	T	C	11: 49,169,118 (GRCm38)	D601G	probably damaging	Het
C9	T	C	15: 6,445,420 (GRCm38)	I20T	possibly damaging	Het
Cacna1b	A	G	2: 24,685,804 (GRCm38)	V744A	probably damaging	Het
Catsper3	A	G	13: 55,808,054 (GRCm38)	E311G	probably damaging	Het
Ccdc180	G	T	4: 45,948,856 (GRCm38)		probably null	Het
Cdc5l	A	G	17: 45,407,846 (GRCm38)	Y615H	probably benign	Het
Crybg2	TGGAGGAGGAGGAGGAGGAG	TGGAGGAGGAGGAGGAG	4: 134,074,526 (GRCm38)		probably benign	Het
Eml4	C	T	17: 83,451,056 (GRCm38)	P502S	probably benign	Het
Fsip1	T	C	2: 118,222,444 (GRCm38)	E367G	probably benign	Het
Gja3	T	C	14: 57,036,714 (GRCm38)	D67G	probably damaging	Het
Gm4894	T	C	9: 49,274,190 (GRCm38)		probably benign	Het
Gm7853	A	G	14: 36,089,527 (GRCm38)		noncoding transcript	Het
Gmps	T	G	3: 64,014,263 (GRCm38)	Y562*	probably null	Het
Golga2	C	A	2: 32,306,465 (GRCm38)	P976T	probably benign	Het
Gpr182	T	A	10: 127,750,141 (GRCm38)	I314F	possibly damaging	Het
Gprc6a	A	G	10: 51,626,795 (GRCm38)	V324A	possibly damaging	Het
Gykl1	A	G	18: 52,695,267 (GRCm38)	T516A	probably benign	Het
Hist2h2ab	T	C	3: 96,220,106 (GRCm38)	L64P	possibly damaging	Het
Ifit1bl2	G	T	19: 34,619,230 (GRCm38)	L329M	possibly damaging	Het
Igsf9b	T	C	9: 27,333,496 (GRCm38)	S920P	probably damaging	Het
Kif3c	T	A	12: 3,366,671 (GRCm38)	S231T	probably benign	Het
Kpna7	A	T	5: 144,989,697 (GRCm38)	Y482N	probably damaging	Het
Lmnb2	T	C	10: 80,904,392 (GRCm38)		probably benign	Het
Lrrc69	T	C	4: 14,773,694 (GRCm38)	S121G	probably benign	Het
Mppe1	A	G	18: 67,228,011 (GRCm38)		probably null	Het
Myh8	T	G	11: 67,308,348 (GRCm38)	N1893K	probably damaging	Het
Myo7a	T	C	7: 98,054,910 (GRCm38)	T1932A	probably benign	Het
Nbn	A	G	4: 15,970,904 (GRCm38)	T296A	probably benign	Het
Nckap1l	T	C	15: 103,455,934 (GRCm38)		probably null	Het
Nek5	A	T	8: 22,113,632 (GRCm38)	N151K	possibly damaging	Het
Nup93	C	T	8: 94,304,191 (GRCm38)	T305I	probably benign	Het
Oca2	T	A	7: 56,357,155 (GRCm38)	H663Q	probably benign	Het
Optn	T	A	2: 5,024,117 (GRCm38)	H525L	probably damaging	Het
Pdzph1	A	G	17: 58,932,412 (GRCm38)		probably benign	Het
Pikfyve	A	G	1: 65,267,855 (GRCm38)	K1801E	probably damaging	Het
Pkd2l2	G	A	18: 34,430,329 (GRCm38)	V478M	probably damaging	Het
Pmepa1	G	A	2: 173,228,133 (GRCm38)	R210W	probably damaging	Het
Ppp1r3c	C	A	19: 36,733,698 (GRCm38)	R224L	probably benign	Het
Prpf19	A	G	19: 10,897,598 (GRCm38)	T39A	probably benign	Het
Pwwp2b	T	C	7: 139,255,188 (GRCm38)	C182R	probably damaging	Het
Rcan3	T	C	4: 135,425,377 (GRCm38)	D11G	probably benign	Het
Rgsl1	C	A	1: 153,822,358 (GRCm38)	W482L	possibly damaging	Het
Sfxn4	C	T	19: 60,851,020 (GRCm38)	G200E	probably damaging	Het
Slc4a8	T	C	15: 100,809,299 (GRCm38)	I848T	probably damaging	Het
Slc7a13	T	C	4: 19,839,399 (GRCm38)	V334A	probably benign	Het
Smarcc2	A	G	10: 128,488,341 (GRCm38)		probably benign	Het
Spata18	A	T	5: 73,666,901 (GRCm38)	I156L	possibly damaging	Het
Spats2	T	C	15: 99,174,453 (GRCm38)		probably null	Het
Tas2r104	G	A	6: 131,685,132 (GRCm38)	H205Y	probably damaging	Het
Tcof1	A	G	18: 60,832,177 (GRCm38)		probably benign	Het
Tex15	C	A	8: 33,571,237 (GRCm38)	H232N	probably benign	Het
Tg	A	T	15: 66,674,011 (GRCm38)	Q194L	probably damaging	Het
Tnfrsf22	C	T	7: 143,644,776 (GRCm38)		probably null	Het
Trim25	T	A	11: 89,016,621 (GRCm38)	V602E	probably damaging	Het
Ttll9	A	G	2: 152,983,063 (GRCm38)	E54G	probably damaging	Het
Ttn	T	C	2: 76,729,324 (GRCm38)	T29578A	probably damaging	Het
Tubgcp5	A	G	7: 55,830,881 (GRCm38)	Q960R	probably damaging	Het
Ugcg	C	T	4: 59,207,798 (GRCm38)	P46S	probably benign	Het
Usp13	A	G	3: 32,917,551 (GRCm38)	I727V	probably benign	Het
Vmn2r67	T	A	7: 85,152,042 (GRCm38)	I229F	probably benign	Het
Vmn2r92	A	C	17: 18,167,392 (GRCm38)	I220L	probably benign	Het
Wnk4	T	A	11: 101,275,641 (GRCm38)		probably benign	Het
Wwp1	A	T	4: 19,641,745 (GRCm38)	Y437N	probably damaging	Het
Zdhhc5	A	G	2: 84,690,213 (GRCm38)	I540T	probably damaging	Het
Zfp729b	T	C	13: 67,595,265 (GRCm38)	I60M	probably damaging	Het

Other mutations in Muc5b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00586:Muc5b	APN	7	141,841,392 (GRCm38)	missense	unknown
IGL00677:Muc5b	APN	7	141,857,894 (GRCm38)	nonsense	probably null
IGL00740:Muc5b	APN	7	141,855,598 (GRCm38)	missense	unknown
IGL01084:Muc5b	APN	7	141,843,449 (GRCm38)	splice site	probably benign
IGL01384:Muc5b	APN	7	141,846,818 (GRCm38)	missense	unknown
IGL01447:Muc5b	APN	7	141,863,094 (GRCm38)	missense	probably benign	0.01
IGL01510:Muc5b	APN	7	141,859,061 (GRCm38)	missense	unknown
IGL01532:Muc5b	APN	7	141,870,006 (GRCm38)	missense	possibly damaging	0.96
IGL01556:Muc5b	APN	7	141,863,240 (GRCm38)	missense	probably benign	0.01
IGL01608:Muc5b	APN	7	141,846,437 (GRCm38)	missense	unknown
IGL01884:Muc5b	APN	7	141,868,083 (GRCm38)	splice site	probably benign
IGL01943:Muc5b	APN	7	141,861,497 (GRCm38)	missense	possibly damaging	0.71
IGL02039:Muc5b	APN	7	141,871,164 (GRCm38)	missense	possibly damaging	0.96
IGL02089:Muc5b	APN	7	141,863,250 (GRCm38)	missense	probably benign	0.04
IGL02110:Muc5b	APN	7	141,847,716 (GRCm38)	nonsense	probably null
IGL02123:Muc5b	APN	7	141,863,757 (GRCm38)	missense	possibly damaging	0.68
IGL02124:Muc5b	APN	7	141,855,632 (GRCm38)	missense	unknown
IGL02141:Muc5b	APN	7	141,853,367 (GRCm38)	missense	unknown
IGL02409:Muc5b	APN	7	141,861,338 (GRCm38)	missense	possibly damaging	0.53
IGL02448:Muc5b	APN	7	141,868,489 (GRCm38)	missense	possibly damaging	0.53
IGL02503:Muc5b	APN	7	141,867,667 (GRCm38)	missense	probably benign	0.33
IGL02504:Muc5b	APN	7	141,846,440 (GRCm38)	missense	unknown
IGL02528:Muc5b	APN	7	141,864,017 (GRCm38)	missense	probably benign	0.01
IGL02534:Muc5b	APN	7	141,844,719 (GRCm38)	missense	unknown
IGL02565:Muc5b	APN	7	141,857,867 (GRCm38)	missense	unknown
IGL02630:Muc5b	APN	7	141,863,231 (GRCm38)	missense	probably benign	0.03
IGL02881:Muc5b	APN	7	141,857,712 (GRCm38)	missense	unknown
IGL02963:Muc5b	APN	7	141,864,264 (GRCm38)	missense	probably damaging	1.00
IGL03003:Muc5b	APN	7	141,863,614 (GRCm38)	missense	probably benign	0.03
IGL03013:Muc5b	APN	7	141,863,928 (GRCm38)	missense	possibly damaging	0.68
IGL03102:Muc5b	APN	7	141,863,069 (GRCm38)	missense	probably benign	0.35
IGL03114:Muc5b	APN	7	141,858,819 (GRCm38)	nonsense	probably null
IGL03150:Muc5b	APN	7	141,865,509 (GRCm38)	missense	possibly damaging	0.53
IGL03185:Muc5b	APN	7	141,862,822 (GRCm38)	missense	possibly damaging	0.83
IGL03299:Muc5b	APN	7	141,841,380 (GRCm38)	missense	unknown
IGL03336:Muc5b	APN	7	141,864,363 (GRCm38)	missense	probably damaging	1.00
IGL03370:Muc5b	APN	7	141,864,777 (GRCm38)	missense	probably benign	0.34
IGL03375:Muc5b	APN	7	141,861,962 (GRCm38)	missense	possibly damaging	0.53
IGL03393:Muc5b	APN	7	141,864,138 (GRCm38)	missense	probably benign	0.21
profligate	UTSW	7	141,857,822 (GRCm38)	nonsense	probably null
wasteful	UTSW	7	141,858,161 (GRCm38)	missense	unknown
R0045:Muc5b	UTSW	7	141,856,818 (GRCm38)	missense	unknown
R0256:Muc5b	UTSW	7	141,843,258 (GRCm38)	missense	unknown
R0256:Muc5b	UTSW	7	141,841,395 (GRCm38)	missense	unknown
R0321:Muc5b	UTSW	7	141,862,235 (GRCm38)	missense	probably benign	0.19
R0391:Muc5b	UTSW	7	141,865,082 (GRCm38)	missense	possibly damaging	0.73
R0458:Muc5b	UTSW	7	141,864,972 (GRCm38)	missense	probably benign	0.20
R0491:Muc5b	UTSW	7	141,862,015 (GRCm38)	missense	probably benign	0.01
R0543:Muc5b	UTSW	7	141,851,785 (GRCm38)	missense	unknown
R0583:Muc5b	UTSW	7	141,856,698 (GRCm38)	nonsense	probably null
R0611:Muc5b	UTSW	7	141,862,436 (GRCm38)	missense	probably benign	0.18
R0625:Muc5b	UTSW	7	141,846,427 (GRCm38)	missense	unknown
R0655:Muc5b	UTSW	7	141,863,942 (GRCm38)	missense	probably benign	0.01
R0845:Muc5b	UTSW	7	141,850,446 (GRCm38)	splice site	probably null
R0863:Muc5b	UTSW	7	141,867,717 (GRCm38)	missense	probably benign	0.18
R0965:Muc5b	UTSW	7	141,863,802 (GRCm38)	missense	possibly damaging	0.92
R0988:Muc5b	UTSW	7	141,871,795 (GRCm38)	missense	probably benign	0.03
R1140:Muc5b	UTSW	7	141,858,996 (GRCm38)	missense	unknown
R1209:Muc5b	UTSW	7	141,857,910 (GRCm38)	missense	unknown
R1333:Muc5b	UTSW	7	141,868,407 (GRCm38)	missense	possibly damaging	0.53
R1337:Muc5b	UTSW	7	141,858,624 (GRCm38)	missense	unknown
R1385:Muc5b	UTSW	7	141,862,137 (GRCm38)	missense	probably benign	0.00
R1463:Muc5b	UTSW	7	141,859,080 (GRCm38)	missense	unknown
R1471:Muc5b	UTSW	7	141,843,234 (GRCm38)	missense	unknown
R1617:Muc5b	UTSW	7	141,863,524 (GRCm38)	nonsense	probably null
R1736:Muc5b	UTSW	7	141,859,107 (GRCm38)	missense	unknown
R1752:Muc5b	UTSW	7	141,867,751 (GRCm38)	missense	possibly damaging	0.96
R1804:Muc5b	UTSW	7	141,863,780 (GRCm38)	missense	possibly damaging	0.68
R1806:Muc5b	UTSW	7	141,865,493 (GRCm38)	missense	possibly damaging	0.68
R1895:Muc5b	UTSW	7	141,857,645 (GRCm38)	missense	unknown
R1902:Muc5b	UTSW	7	141,864,105 (GRCm38)	missense	possibly damaging	0.77
R1919:Muc5b	UTSW	7	141,846,031 (GRCm38)	missense	unknown
R1924:Muc5b	UTSW	7	141,868,223 (GRCm38)	missense	possibly damaging	0.53
R1942:Muc5b	UTSW	7	141,857,684 (GRCm38)	missense	unknown
R1959:Muc5b	UTSW	7	141,862,637 (GRCm38)	missense	possibly damaging	0.86
R1960:Muc5b	UTSW	7	141,862,637 (GRCm38)	missense	possibly damaging	0.86
R1976:Muc5b	UTSW	7	141,863,154 (GRCm38)	missense	probably benign	0.01
R2080:Muc5b	UTSW	7	141,869,754 (GRCm38)	missense	probably benign	0.33
R2178:Muc5b	UTSW	7	141,864,116 (GRCm38)	missense	possibly damaging	0.58
R2184:Muc5b	UTSW	7	141,858,864 (GRCm38)	nonsense	probably null
R2237:Muc5b	UTSW	7	141,862,089 (GRCm38)	missense	probably benign	0.00
R2509:Muc5b	UTSW	7	141,859,061 (GRCm38)	missense	unknown
R2510:Muc5b	UTSW	7	141,859,061 (GRCm38)	missense	unknown
R2512:Muc5b	UTSW	7	141,859,076 (GRCm38)	missense	unknown
R2888:Muc5b	UTSW	7	141,861,554 (GRCm38)	missense	probably damaging	0.98
R3054:Muc5b	UTSW	7	141,864,041 (GRCm38)	missense	probably damaging	0.97
R3055:Muc5b	UTSW	7	141,864,041 (GRCm38)	missense	probably damaging	0.97
R3108:Muc5b	UTSW	7	141,858,759 (GRCm38)	missense	unknown
R3109:Muc5b	UTSW	7	141,858,759 (GRCm38)	missense	unknown
R3113:Muc5b	UTSW	7	141,846,134 (GRCm38)	missense	unknown
R3551:Muc5b	UTSW	7	141,861,335 (GRCm38)	missense	possibly damaging	0.53
R3552:Muc5b	UTSW	7	141,867,705 (GRCm38)	missense	probably benign	0.18
R3552:Muc5b	UTSW	7	141,861,335 (GRCm38)	missense	possibly damaging	0.53
R3622:Muc5b	UTSW	7	141,851,858 (GRCm38)	splice site	probably benign
R3700:Muc5b	UTSW	7	141,847,249 (GRCm38)	missense	unknown
R3734:Muc5b	UTSW	7	141,859,037 (GRCm38)	nonsense	probably null
R3785:Muc5b	UTSW	7	141,865,116 (GRCm38)	missense	possibly damaging	0.86
R3786:Muc5b	UTSW	7	141,865,116 (GRCm38)	missense	possibly damaging	0.86
R3788:Muc5b	UTSW	7	141,863,834 (GRCm38)	missense	possibly damaging	0.68
R3810:Muc5b	UTSW	7	141,864,126 (GRCm38)	missense	possibly damaging	0.58
R3834:Muc5b	UTSW	7	141,859,181 (GRCm38)	missense	unknown
R3835:Muc5b	UTSW	7	141,859,181 (GRCm38)	missense	unknown
R3850:Muc5b	UTSW	7	141,862,638 (GRCm38)	missense	possibly damaging	0.95
R3877:Muc5b	UTSW	7	141,857,552 (GRCm38)	missense	unknown
R3909:Muc5b	UTSW	7	141,849,498 (GRCm38)	missense	unknown
R3964:Muc5b	UTSW	7	141,866,968 (GRCm38)	missense	possibly damaging	0.73
R4014:Muc5b	UTSW	7	141,863,630 (GRCm38)	missense	probably benign	0.40
R4015:Muc5b	UTSW	7	141,863,630 (GRCm38)	missense	probably benign	0.40
R4017:Muc5b	UTSW	7	141,863,630 (GRCm38)	missense	probably benign	0.40
R4042:Muc5b	UTSW	7	141,864,887 (GRCm38)	missense	possibly damaging	0.92
R4200:Muc5b	UTSW	7	141,858,925 (GRCm38)	nonsense	probably null
R4230:Muc5b	UTSW	7	141,863,522 (GRCm38)	missense	probably benign	0.03
R4400:Muc5b	UTSW	7	141,861,387 (GRCm38)	missense	possibly damaging	0.92
R4455:Muc5b	UTSW	7	141,858,818 (GRCm38)	missense	unknown
R4484:Muc5b	UTSW	7	141,868,450 (GRCm38)	missense	possibly damaging	0.73
R4630:Muc5b	UTSW	7	141,857,984 (GRCm38)	missense	unknown
R4646:Muc5b	UTSW	7	141,862,640 (GRCm38)	missense	probably benign	0.34
R4658:Muc5b	UTSW	7	141,841,398 (GRCm38)	missense	unknown
R4667:Muc5b	UTSW	7	141,842,379 (GRCm38)	missense	unknown
R4690:Muc5b	UTSW	7	141,842,294 (GRCm38)	missense	unknown
R4697:Muc5b	UTSW	7	141,857,361 (GRCm38)	missense	unknown
R4711:Muc5b	UTSW	7	141,846,033 (GRCm38)	missense	unknown
R4713:Muc5b	UTSW	7	141,849,079 (GRCm38)	nonsense	probably null
R4749:Muc5b	UTSW	7	141,861,448 (GRCm38)	nonsense	probably null
R4753:Muc5b	UTSW	7	141,856,853 (GRCm38)	missense	unknown
R4782:Muc5b	UTSW	7	141,847,716 (GRCm38)	nonsense	probably null
R4795:Muc5b	UTSW	7	141,849,567 (GRCm38)	missense	unknown
R4796:Muc5b	UTSW	7	141,864,246 (GRCm38)	missense	possibly damaging	0.52
R4799:Muc5b	UTSW	7	141,847,716 (GRCm38)	nonsense	probably null
R4824:Muc5b	UTSW	7	141,864,185 (GRCm38)	missense	probably damaging	1.00
R4825:Muc5b	UTSW	7	141,868,465 (GRCm38)	missense	possibly damaging	0.96
R5068:Muc5b	UTSW	7	141,858,608 (GRCm38)	missense	unknown
R5073:Muc5b	UTSW	7	141,859,262 (GRCm38)	missense	unknown
R5074:Muc5b	UTSW	7	141,859,262 (GRCm38)	missense	unknown
R5107:Muc5b	UTSW	7	141,855,531 (GRCm38)	missense	unknown
R5152:Muc5b	UTSW	7	141,865,531 (GRCm38)	missense	possibly damaging	0.53
R5183:Muc5b	UTSW	7	141,850,810 (GRCm38)	missense	unknown
R5191:Muc5b	UTSW	7	141,858,539 (GRCm38)	missense	unknown
R5254:Muc5b	UTSW	7	141,864,540 (GRCm38)	missense	probably benign	0.09
R5320:Muc5b	UTSW	7	141,859,001 (GRCm38)	missense	unknown
R5352:Muc5b	UTSW	7	141,864,558 (GRCm38)	missense	possibly damaging	0.66
R5378:Muc5b	UTSW	7	141,862,203 (GRCm38)	missense	unknown
R5417:Muc5b	UTSW	7	141,858,044 (GRCm38)	missense	unknown
R5548:Muc5b	UTSW	7	141,863,942 (GRCm38)	missense	probably benign	0.01
R5551:Muc5b	UTSW	7	141,868,503 (GRCm38)	missense	possibly damaging	0.73
R5562:Muc5b	UTSW	7	141,847,238 (GRCm38)	missense	unknown
R5580:Muc5b	UTSW	7	141,861,347 (GRCm38)	missense	possibly damaging	0.53
R5629:Muc5b	UTSW	7	141,861,299 (GRCm38)	missense	possibly damaging	0.73
R5758:Muc5b	UTSW	7	141,858,983 (GRCm38)	missense	unknown
R5783:Muc5b	UTSW	7	141,858,428 (GRCm38)	nonsense	probably null
R5795:Muc5b	UTSW	7	141,871,741 (GRCm38)	missense	possibly damaging	0.96
R5796:Muc5b	UTSW	7	141,857,396 (GRCm38)	missense	unknown
R5797:Muc5b	UTSW	7	141,851,582 (GRCm38)	missense	unknown
R5806:Muc5b	UTSW	7	141,862,835 (GRCm38)	missense	possibly damaging	0.68
R5888:Muc5b	UTSW	7	141,858,421 (GRCm38)	missense	unknown
R5910:Muc5b	UTSW	7	141,861,311 (GRCm38)	missense	possibly damaging	0.53
R5956:Muc5b	UTSW	7	141,864,173 (GRCm38)	missense	probably damaging	0.99
R5970:Muc5b	UTSW	7	141,856,712 (GRCm38)	missense	unknown
R5990:Muc5b	UTSW	7	141,858,161 (GRCm38)	missense	unknown
R5999:Muc5b	UTSW	7	141,857,379 (GRCm38)	missense	unknown
R6001:Muc5b	UTSW	7	141,872,381 (GRCm38)	missense	possibly damaging	0.72
R6053:Muc5b	UTSW	7	141,864,708 (GRCm38)	missense	probably benign	0.07
R6073:Muc5b	UTSW	7	141,858,288 (GRCm38)	missense	unknown
R6073:Muc5b	UTSW	7	141,849,060 (GRCm38)	missense	unknown
R6112:Muc5b	UTSW	7	141,863,305 (GRCm38)	missense	probably benign	0.01
R6153:Muc5b	UTSW	7	141,861,444 (GRCm38)	missense	possibly damaging	0.71
R6164:Muc5b	UTSW	7	141,863,345 (GRCm38)	missense	possibly damaging	0.73
R6172:Muc5b	UTSW	7	141,858,776 (GRCm38)	missense	unknown
R6178:Muc5b	UTSW	7	141,856,342 (GRCm38)	missense	probably null
R6196:Muc5b	UTSW	7	141,851,596 (GRCm38)	missense	unknown
R6213:Muc5b	UTSW	7	141,867,619 (GRCm38)	missense	possibly damaging	0.92
R6213:Muc5b	UTSW	7	141,862,166 (GRCm38)	missense	probably benign	0.00
R6344:Muc5b	UTSW	7	141,862,971 (GRCm38)	missense	possibly damaging	0.62
R6400:Muc5b	UTSW	7	141,858,665 (GRCm38)	missense	unknown
R6414:Muc5b	UTSW	7	141,859,097 (GRCm38)	missense	unknown
R6521:Muc5b	UTSW	7	141,859,171 (GRCm38)	nonsense	probably null
R6658:Muc5b	UTSW	7	141,868,507 (GRCm38)	critical splice donor site	probably null
R6717:Muc5b	UTSW	7	141,857,822 (GRCm38)	nonsense	probably null
R6737:Muc5b	UTSW	7	141,857,499 (GRCm38)	missense	unknown
R6763:Muc5b	UTSW	7	141,862,284 (GRCm38)	missense	probably benign	0.01
R6817:Muc5b	UTSW	7	141,862,913 (GRCm38)	missense	probably benign	0.06
R6819:Muc5b	UTSW	7	141,858,863 (GRCm38)	missense	unknown
R6916:Muc5b	UTSW	7	141,864,717 (GRCm38)	missense	possibly damaging	0.71
R7030:Muc5b	UTSW	7	141,842,455 (GRCm38)	missense	unknown
R7116:Muc5b	UTSW	7	141,863,750 (GRCm38)	missense	probably benign	0.10
R7134:Muc5b	UTSW	7	141,857,654 (GRCm38)	missense	unknown
R7146:Muc5b	UTSW	7	141,863,967 (GRCm38)	missense	possibly damaging	0.96
R7168:Muc5b	UTSW	7	141,864,017 (GRCm38)	missense	probably benign	0.01
R7182:Muc5b	UTSW	7	141,842,645 (GRCm38)	missense	unknown
R7189:Muc5b	UTSW	7	141,861,061 (GRCm38)	nonsense	probably null
R7207:Muc5b	UTSW	7	141,862,865 (GRCm38)	missense	probably benign	0.01
R7232:Muc5b	UTSW	7	141,866,129 (GRCm38)	missense	possibly damaging	0.53
R7260:Muc5b	UTSW	7	141,842,648 (GRCm38)	missense	unknown
R7269:Muc5b	UTSW	7	141,857,535 (GRCm38)	missense	unknown
R7273:Muc5b	UTSW	7	141,851,570 (GRCm38)	missense	unknown
R7278:Muc5b	UTSW	7	141,857,502 (GRCm38)	missense	unknown
R7307:Muc5b	UTSW	7	141,842,294 (GRCm38)	missense	unknown
R7323:Muc5b	UTSW	7	141,858,707 (GRCm38)	missense	unknown
R7374:Muc5b	UTSW	7	141,863,126 (GRCm38)	missense	probably benign	0.10
R7376:Muc5b	UTSW	7	141,872,550 (GRCm38)	missense	possibly damaging	0.53
R7382:Muc5b	UTSW	7	141,858,948 (GRCm38)	missense	unknown
R7481:Muc5b	UTSW	7	141,861,171 (GRCm38)	missense	unknown
R7497:Muc5b	UTSW	7	141,861,513 (GRCm38)	missense	possibly damaging	0.92
R7554:Muc5b	UTSW	7	141,858,776 (GRCm38)	missense	unknown
R7571:Muc5b	UTSW	7	141,847,249 (GRCm38)	missense	unknown
R7598:Muc5b	UTSW	7	141,859,262 (GRCm38)	missense	unknown
R7609:Muc5b	UTSW	7	141,861,729 (GRCm38)	missense	possibly damaging	0.86
R7615:Muc5b	UTSW	7	141,864,892 (GRCm38)	nonsense	probably null
R7618:Muc5b	UTSW	7	141,867,597 (GRCm38)	missense	probably benign	0.01
R7651:Muc5b	UTSW	7	141,864,023 (GRCm38)	missense	possibly damaging	0.75
R7692:Muc5b	UTSW	7	141,853,229 (GRCm38)	missense	unknown
R7731:Muc5b	UTSW	7	141,857,305 (GRCm38)	critical splice acceptor site	probably null
R7746:Muc5b	UTSW	7	141,862,239 (GRCm38)	missense	probably benign	0.10
R7748:Muc5b	UTSW	7	141,847,805 (GRCm38)	missense	unknown
R7774:Muc5b	UTSW	7	141,842,379 (GRCm38)	missense	unknown
R7783:Muc5b	UTSW	7	141,857,341 (GRCm38)	missense	unknown
R7834:Muc5b	UTSW	7	141,859,070 (GRCm38)	missense	unknown
R7872:Muc5b	UTSW	7	141,846,113 (GRCm38)	missense	unknown
R7935:Muc5b	UTSW	7	141,846,832 (GRCm38)	missense	unknown
R8026:Muc5b	UTSW	7	141,863,636 (GRCm38)	missense	probably benign	0.03
R8036:Muc5b	UTSW	7	141,867,741 (GRCm38)	missense	possibly damaging	0.73
R8081:Muc5b	UTSW	7	141,864,006 (GRCm38)	missense	possibly damaging	0.88
R8096:Muc5b	UTSW	7	141,849,555 (GRCm38)	missense	unknown
R8101:Muc5b	UTSW	7	141,865,175 (GRCm38)	missense	possibly damaging	0.53
R8112:Muc5b	UTSW	7	141,862,028 (GRCm38)	missense	possibly damaging	0.53
R8131:Muc5b	UTSW	7	141,842,409 (GRCm38)	missense	unknown
R8170:Muc5b	UTSW	7	141,861,000 (GRCm38)	missense	unknown
R8171:Muc5b	UTSW	7	141,861,000 (GRCm38)	missense	unknown
R8191:Muc5b	UTSW	7	141,867,684 (GRCm38)	missense	probably benign	0.18
R8237:Muc5b	UTSW	7	141,857,960 (GRCm38)	missense	unknown
R8342:Muc5b	UTSW	7	141,860,865 (GRCm38)	missense	unknown
R8343:Muc5b	UTSW	7	141,864,161 (GRCm38)	missense	probably benign	0.28
R8389:Muc5b	UTSW	7	141,861,779 (GRCm38)	missense	possibly damaging	0.53
R8396:Muc5b	UTSW	7	141,851,815 (GRCm38)	missense	unknown
R8733:Muc5b	UTSW	7	141,863,795 (GRCm38)	missense	possibly damaging	0.68
R8774:Muc5b	UTSW	7	141,865,094 (GRCm38)	missense	probably benign	0.18
R8774-TAIL:Muc5b	UTSW	7	141,865,094 (GRCm38)	missense	probably benign	0.18
R8833:Muc5b	UTSW	7	141,858,368 (GRCm38)	missense	unknown
R8884:Muc5b	UTSW	7	141,849,419 (GRCm38)	missense	unknown
R8907:Muc5b	UTSW	7	141,864,138 (GRCm38)	missense	probably benign	0.21
R8944:Muc5b	UTSW	7	141,867,378 (GRCm38)	missense
R9025:Muc5b	UTSW	7	141,872,472 (GRCm38)	missense	probably damaging	0.98
R9044:Muc5b	UTSW	7	141,858,058 (GRCm38)	missense	unknown
R9117:Muc5b	UTSW	7	141,869,333 (GRCm38)	missense	possibly damaging	0.73
R9145:Muc5b	UTSW	7	141,857,613 (GRCm38)	missense	unknown
R9154:Muc5b	UTSW	7	141,864,237 (GRCm38)	missense	probably damaging	0.98
R9177:Muc5b	UTSW	7	141,845,338 (GRCm38)	missense	unknown
R9190:Muc5b	UTSW	7	141,858,202 (GRCm38)	missense	unknown
R9204:Muc5b	UTSW	7	141,856,392 (GRCm38)	missense	unknown
R9260:Muc5b	UTSW	7	141,851,518 (GRCm38)	missense	unknown
R9331:Muc5b	UTSW	7	141,857,738 (GRCm38)	missense	unknown
R9366:Muc5b	UTSW	7	141,863,304 (GRCm38)	missense	probably benign	0.01
R9402:Muc5b	UTSW	7	141,845,414 (GRCm38)	missense	unknown
R9462:Muc5b	UTSW	7	141,861,479 (GRCm38)	missense
R9463:Muc5b	UTSW	7	141,851,766 (GRCm38)	missense	unknown
R9490:Muc5b	UTSW	7	141,871,760 (GRCm38)	missense	probably benign	0.33
R9523:Muc5b	UTSW	7	141,842,379 (GRCm38)	missense	unknown
R9548:Muc5b	UTSW	7	141,867,911 (GRCm38)	missense	possibly damaging	0.52
R9591:Muc5b	UTSW	7	141,858,779 (GRCm38)	missense	unknown
R9602:Muc5b	UTSW	7	141,863,474 (GRCm38)	missense	probably benign	0.11
R9664:Muc5b	UTSW	7	141,855,542 (GRCm38)	missense	unknown
R9703:Muc5b	UTSW	7	141,871,798 (GRCm38)	missense	possibly damaging	0.53
R9713:Muc5b	UTSW	7	141,862,941 (GRCm38)	missense	probably benign	0.01
R9789:Muc5b	UTSW	7	141,861,593 (GRCm38)	missense	possibly damaging	0.95
R9800:Muc5b	UTSW	7	141,861,743 (GRCm38)	missense	possibly damaging	0.53
Z1088:Muc5b	UTSW	7	141,862,214 (GRCm38)	missense	probably benign	0.01
Z1176:Muc5b	UTSW	7	141,842,705 (GRCm38)	missense	unknown
Z1177:Muc5b	UTSW	7	141,863,173 (GRCm38)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- AACCTGGCCAGTTTGAAGGAG -3'
(R):5'- AGACAATCTTTTCTGTCCTGGGG -3'

Sequencing Primer
(F):5'- CTGGCCAGTTTGAAGGAGACATC -3'
(R):5'- CAATCTTTTCTGTCCTGGGGGTAGAG -3'

Posted On

2014-10-15