Mutagenetix > Incidental Mutations

Incidental Mutation 'R2225:Zfp451'

239646

Institutional Source

Beutler Lab

Gene Symbol

Zfp451

Ensembl Gene

ENSMUSG00000042197

Gene Name

zinc finger protein 451

Synonyms

4930515K21Rik, Kiaa0576-hp, 4933435G09Rik

MMRRC Submission

040226-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2225 (G1)

Quality Score

209

Status

Validated

Chromosome

Chromosomal Location

33761545-33814595 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 33770907 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000137992 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019861] [ENSMUST00000139143]

Predicted Effect

probably benign
Transcript: ENSMUST00000019861

SMART Domains

Protein: ENSMUSP00000019861
Gene: ENSMUSG00000042197

Domain	Start	End	E-Value	Type
coiled coil region	81	109	N/A	INTRINSIC
ZnF_C2H2	169	195	1.63e1	SMART
ZnF_C2H2	212	232	1.18e2	SMART
ZnF_C2H2	253	277	1.73e0	SMART
ZnF_C2H2	315	335	2.03e2	SMART
ZnF_C2H2	362	385	3.75e1	SMART
ZnF_C2H2	494	517	2.91e-2	SMART
ZnF_C2H2	527	550	5.4e1	SMART
low complexity region	558	577	N/A	INTRINSIC
ZnF_C2H2	604	629	1.55e1	SMART
ZnF_C2H2	634	657	2.29e0	SMART
ZnF_C2H2	665	687	1.64e-1	SMART
ZnF_C2H2	751	774	6.75e0	SMART
ZnF_C2H2	787	810	4.94e0	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000139143

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abtb1	A	C	6: 88,836,367	L439R	probably damaging	Het
Adcy10	A	G	1: 165,518,260	T293A	probably damaging	Het
Aen	C	A	7: 78,902,451	T15K	probably benign	Het
Aggf1	A	G	13: 95,370,846	S144P	probably damaging	Het
Akap9	A	T	5: 4,077,271	R3706S	probably damaging	Het
Btd	A	G	14: 31,667,060	D246G	probably benign	Het
Ccdc33	A	G	9: 58,082,022	S123P	probably damaging	Het
Cep152	T	C	2: 125,581,784	E899G	probably damaging	Het
Cpsf6	A	T	10: 117,363,036		probably benign	Het
Crybg3	A	G	16: 59,554,678	I2071T	probably damaging	Het
Cwc22	T	A	2: 77,908,151		probably benign	Het
Ddah2	T	A	17: 35,060,211	I16N	probably damaging	Het
Dld	T	G	12: 31,341,449	M123L	probably benign	Het
Eif5b	A	T	1: 38,019,223	K202I	unknown	Het
Gm6430	T	C	1: 97,025,716		noncoding transcript	Het
Hdac9	C	T	12: 34,407,802	V251I	probably benign	Het
Hoxa10	T	C	6: 52,232,636	E52G	probably damaging	Het
Hspa4	C	T	11: 53,286,933	V117M	probably benign	Het
Htra4	T	C	8: 25,025,720	D434G	probably benign	Het
Itgb3	A	G	11: 104,665,510	M726V	probably benign	Het
Itih1	A	G	14: 30,929,577	V886A	possibly damaging	Het
Kdm1b	TCATTGTCC	TCATTGTCCATTGTCC	13: 47,064,088		probably null	Het
Kpna1	G	A	16: 36,031,221	A392T	probably damaging	Het
Limk1	G	T	5: 134,661,556		probably null	Het
Lonrf1	T	C	8: 36,236,098	D308G	probably damaging	Het
Muc4	A	T	16: 32,755,891		probably benign	Het
Muc4	G	A	16: 32,766,942	C2719Y	possibly damaging	Het
Mvb12b	G	A	2: 33,840,199	T60I	possibly damaging	Het
Myh2	C	T	11: 67,193,729	T1698M	probably benign	Het
Naalad2	A	T	9: 18,376,533	V267E	possibly damaging	Het
Nkpd1	A	T	7: 19,519,820	Y37F	probably benign	Het
Olfr167	T	C	16: 19,515,246	H130R	probably benign	Het
Olfr952	T	C	9: 39,426,619	I151V	probably benign	Het
Olfr975	T	C	9: 39,950,537	K78R	possibly damaging	Het
Plekhg2	G	A	7: 28,360,335	P1190S	probably benign	Het
Reck	A	G	4: 43,922,837	T371A	probably benign	Het
Rnf185	T	C	11: 3,432,445	D44G	probably damaging	Het
Rpgrip1l	T	C	8: 91,221,467	E1196G	probably benign	Het
Rundc1	A	G	11: 101,431,344		probably benign	Het
Sf3b3	T	C	8: 110,814,573	Y970C	probably damaging	Het
Sfxn1	A	G	13: 54,085,517	T20A	possibly damaging	Het
Shprh	T	C	10: 11,162,235		probably benign	Het
Spata31d1a	T	A	13: 59,703,715	I200L	probably benign	Het
Srsf6	G	A	2: 162,931,699	S10N	probably damaging	Het
Tbxa2r	A	G	10: 81,333,149	Y224C	probably benign	Het
Tlr5	A	G	1: 182,972,376		probably benign	Het
Tmem270	A	G	5: 134,906,638	L21P	probably damaging	Het
Tmtc2	T	C	10: 105,370,357	E359G	probably benign	Het
Tnn	A	T	1: 160,147,465	C131S	probably damaging	Het
Tnnt2	A	G	1: 135,843,791		probably benign	Het
Tns2	C	T	15: 102,108,934	R281C	probably damaging	Het
Tph1	A	G	7: 46,665,174		probably null	Het
Wiz	C	T	17: 32,356,925	V836M	probably damaging	Het
Zfp738	A	G	13: 67,670,312	F520S	probably damaging	Het

Other mutations in Zfp451

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00225:Zfp451	APN	1	33786540	intron	probably benign
IGL00423:Zfp451	APN	1	33777579	missense	probably benign	0.44
IGL00925:Zfp451	APN	1	33776261	unclassified	probably benign
IGL00971:Zfp451	APN	1	33783153	missense	probably benign	0.01
IGL01521:Zfp451	APN	1	33777331	splice site	probably null
IGL01672:Zfp451	APN	1	33762166	missense	probably benign	0.33
IGL01826:Zfp451	APN	1	33782162	missense	probably damaging	1.00
IGL02298:Zfp451	APN	1	33772921	missense	probably damaging	0.98
IGL02343:Zfp451	APN	1	33776493	missense	probably damaging	1.00
IGL03150:Zfp451	APN	1	33777454	missense	probably damaging	1.00
IGL03257:Zfp451	APN	1	33777048	missense	possibly damaging	0.90
R0006:Zfp451	UTSW	1	33802780	intron	probably benign
R0068:Zfp451	UTSW	1	33777625	missense	probably damaging	1.00
R0068:Zfp451	UTSW	1	33777625	missense	probably damaging	1.00
R0358:Zfp451	UTSW	1	33777729	missense	probably damaging	1.00
R0441:Zfp451	UTSW	1	33777045	missense	probably damaging	0.96
R0483:Zfp451	UTSW	1	33770910	splice site	probably benign
R0745:Zfp451	UTSW	1	33770848	nonsense	probably null
R1469:Zfp451	UTSW	1	33769813	missense	possibly damaging	0.93
R1469:Zfp451	UTSW	1	33769813	missense	possibly damaging	0.93
R1486:Zfp451	UTSW	1	33777727	missense	probably damaging	0.99
R1774:Zfp451	UTSW	1	33813768	missense	probably benign	0.02
R1929:Zfp451	UTSW	1	33782193	missense	probably damaging	1.00
R1929:Zfp451	UTSW	1	33783856	missense	probably benign	0.12
R1933:Zfp451	UTSW	1	33777822	missense	probably damaging	1.00
R2108:Zfp451	UTSW	1	33779167	missense	possibly damaging	0.93
R2372:Zfp451	UTSW	1	33780052	splice site	probably null
R3923:Zfp451	UTSW	1	33779045	missense	probably null	1.00
R4295:Zfp451	UTSW	1	33777755	missense	probably damaging	0.99
R4409:Zfp451	UTSW	1	33777413	missense	probably damaging	1.00
R4617:Zfp451	UTSW	1	33802671	intron	probably benign
R4757:Zfp451	UTSW	1	33765858	missense	probably damaging	0.98
R4777:Zfp451	UTSW	1	33782105	missense	possibly damaging	0.80
R4906:Zfp451	UTSW	1	33805384	missense	probably damaging	1.00
R4964:Zfp451	UTSW	1	33777861	missense	probably damaging	1.00
R5128:Zfp451	UTSW	1	33802933	intron	probably benign
R5129:Zfp451	UTSW	1	33802933	intron	probably benign
R5383:Zfp451	UTSW	1	33813806	missense	probably damaging	1.00
R5446:Zfp451	UTSW	1	33777528	missense	probably damaging	1.00
R6154:Zfp451	UTSW	1	33803546	intron	probably benign
R6228:Zfp451	UTSW	1	33803138	intron	probably benign
R6272:Zfp451	UTSW	1	33803244	intron	probably benign
R6296:Zfp451	UTSW	1	33769817	nonsense	probably null
R6321:Zfp451	UTSW	1	33813735	missense	probably damaging	1.00
R6445:Zfp451	UTSW	1	33773011	missense	probably damaging	1.00
R6528:Zfp451	UTSW	1	33777781	missense	probably damaging	1.00
R6562:Zfp451	UTSW	1	33762179	missense	possibly damaging	0.90
R6739:Zfp451	UTSW	1	33803594	intron	probably benign
R6911:Zfp451	UTSW	1	33803456	intron	probably benign
R7042:Zfp451	UTSW	1	33777393	missense	probably damaging	1.00
R7044:Zfp451	UTSW	1	33802167	intron	probably benign
R7071:Zfp451	UTSW	1	33776744	missense	possibly damaging	0.96
R7082:Zfp451	UTSW	1	33772891	critical splice donor site	probably null
R7123:Zfp451	UTSW	1	33776869	missense	probably damaging	1.00
R7149:Zfp451	UTSW	1	33777324	missense	probably damaging	1.00
R7179:Zfp451	UTSW	1	33802570	missense	unknown
R7185:Zfp451	UTSW	1	33769893	missense	probably damaging	1.00
R7228:Zfp451	UTSW	1	33803394	missense	unknown
R7402:Zfp451	UTSW	1	33813762	missense	probably benign
R7462:Zfp451	UTSW	1	33777013	missense	probably damaging	1.00
R7488:Zfp451	UTSW	1	33779140	missense	probably benign	0.22
R7507:Zfp451	UTSW	1	33769759	missense	probably damaging	1.00
R7774:Zfp451	UTSW	1	33805393	missense	probably benign	0.20
R7835:Zfp451	UTSW	1	33772979	missense	probably damaging	1.00
R7979:Zfp451	UTSW	1	33782138	missense	probably benign	0.01
R8123:Zfp451	UTSW	1	33762167	missense	possibly damaging	0.92
R8137:Zfp451	UTSW	1	33782075	missense	possibly damaging	0.57
RF005:Zfp451	UTSW	1	33776792	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CATGCAGACACATAGAGCTTGAC -3'
(R):5'- TGTTCCAGGACTGAGATAGCATG -3'

Sequencing Primer
(F):5'- GACACATAGAGCTTGACATCAGTC -3'
(R):5'- ATGCGTATGAACTACCAGGCCTG -3'

Posted On

2014-10-15