Mutagenetix > Incidental Mutation

Incidental Mutation 'R2227:Vmn1r86'

239788

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r86

Ensembl Gene

ENSMUSG00000070816

Gene Name

vomeronasal 1 receptor 86

Synonyms

Gm10301

MMRRC Submission

040228-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R2227 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

12835924-12836874 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 12836847 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 10 (I10V)

Ref Sequence

ENSEMBL: ENSMUSP00000154549 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094828] [ENSMUST00000226604] [ENSMUST00000227220] [ENSMUST00000227700]

AlphaFold

L7N213

Predicted Effect

probably benign
Transcript: ENSMUST00000094828
AA Change: I10V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000092423
Gene: ENSMUSG00000070816
AA Change: I10V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	306	1.1e-14	PFAM
Pfam:V1R	35	301	4e-31	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210729

Predicted Effect

probably benign
Transcript: ENSMUST00000226604
AA Change: I10V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000227220
AA Change: I10V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000227700

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563I02Rik	T	A	14: 60,325,668 (GRCm39)		probably benign	Het
Abtb1	A	C	6: 88,813,349 (GRCm39)	L439R	probably damaging	Het
Adcy10	A	G	1: 165,345,829 (GRCm39)	T293A	probably damaging	Het
Agl	A	T	3: 116,581,961 (GRCm39)	V306D	possibly damaging	Het
Alpk2	T	A	18: 65,511,147 (GRCm39)	T17S	probably damaging	Het
Ar	T	A	X: 97,194,937 (GRCm39)	M517K	probably benign	Het
Atg9b	A	G	5: 24,591,393 (GRCm39)	V735A	possibly damaging	Het
BB014433	C	A	8: 15,091,717 (GRCm39)	A379S	probably benign	Het
Ccdc33	A	G	9: 57,989,305 (GRCm39)	S123P	probably damaging	Het
Ccl2	G	T	11: 81,927,427 (GRCm39)		probably null	Het
Cct2	T	C	10: 116,888,922 (GRCm39)	R526G	probably null	Het
Cep55	T	C	19: 38,051,082 (GRCm39)	M164T	probably benign	Het
Clec2d	G	A	6: 129,161,214 (GRCm39)	A104T	probably benign	Het
Cyp3a11	A	G	5: 145,805,357 (GRCm39)	L220P	possibly damaging	Het
Dgkk	T	A	X: 6,741,487 (GRCm39)	D102E	probably damaging	Het
Emcn	T	C	3: 137,109,778 (GRCm39)	I140T	possibly damaging	Het
Exoc2	T	A	13: 31,048,867 (GRCm39)	I729L	probably benign	Het
Fbxl20	T	C	11: 97,981,675 (GRCm39)	I338V	probably benign	Het
Gucy2c	G	A	6: 136,679,758 (GRCm39)	T943I	probably damaging	Het
Hdac9	C	T	12: 34,457,801 (GRCm39)	V251I	probably benign	Het
Hoxa10	T	C	6: 52,209,616 (GRCm39)	E52G	probably damaging	Het
Il17rb	A	T	14: 29,728,038 (GRCm39)	S56R	probably benign	Het
Knl1	A	G	2: 118,902,481 (GRCm39)	D1394G	probably damaging	Het
Kpna1	G	A	16: 35,851,591 (GRCm39)	A392T	probably damaging	Het
Krt6b	A	G	15: 101,587,557 (GRCm39)	V179A	probably damaging	Het
Lpar5	A	T	6: 125,058,098 (GRCm39)		probably null	Het
Marchf7	A	G	2: 60,060,190 (GRCm39)	R106G	probably benign	Het
Mtus1	C	T	8: 41,535,812 (GRCm39)	V635M	probably damaging	Het
Myl1	T	C	1: 66,983,974 (GRCm39)	K31E	possibly damaging	Het
Myt1l	T	A	12: 29,876,969 (GRCm39)	L207I	unknown	Het
Naalad2	A	T	9: 18,287,829 (GRCm39)	V267E	possibly damaging	Het
Pkhd1l1	T	C	15: 44,376,188 (GRCm39)	I950T	possibly damaging	Het
Ppp1r9a	G	T	6: 5,154,074 (GRCm39)	R1081L	probably benign	Het
Rc3h1	T	C	1: 160,791,112 (GRCm39)	I932T	probably benign	Het
Rubcnl	G	A	14: 75,279,832 (GRCm39)	R405Q	probably benign	Het
Saxo5	A	G	8: 3,529,249 (GRCm39)	T245A	probably benign	Het
Sgsm3	A	G	15: 80,888,069 (GRCm39)	E53G	probably damaging	Het
Slco1a8	T	G	6: 141,938,087 (GRCm39)	E277D	possibly damaging	Het
Spin2g	A	T	X: 33,656,599 (GRCm39)	I171N	possibly damaging	Het
Srsf6	G	A	2: 162,773,619 (GRCm39)	S10N	probably damaging	Het
Tesl2	T	A	X: 23,825,173 (GRCm39)	M1L	probably null	Het
Thoc2l	T	A	5: 104,667,286 (GRCm39)	Y603N	probably damaging	Het
Tnn	A	T	1: 159,975,035 (GRCm39)	C131S	probably damaging	Het
Tnnt2	A	G	1: 135,771,529 (GRCm39)		probably benign	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Xpot	T	C	10: 121,458,765 (GRCm39)	R20G	probably damaging	Het
Zfp735	T	A	11: 73,602,222 (GRCm39)	L389I	possibly damaging	Het
Zfp735	T	G	11: 73,602,223 (GRCm39)	L389*	probably null	Het

Other mutations in Vmn1r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Vmn1r86	APN	7	12,836,468 (GRCm39)	missense	possibly damaging	0.91
IGL01124:Vmn1r86	APN	7	12,836,856 (GRCm39)	missense	probably benign	0.04
IGL01291:Vmn1r86	APN	7	12,836,433 (GRCm39)	missense	probably benign	0.14
IGL02347:Vmn1r86	APN	7	12,836,574 (GRCm39)	missense	probably damaging	0.99
IGL02457:Vmn1r86	APN	7	12,836,707 (GRCm39)	missense	probably benign
IGL02983:Vmn1r86	APN	7	12,836,741 (GRCm39)	missense	probably damaging	1.00
ANU05:Vmn1r86	UTSW	7	12,836,433 (GRCm39)	missense	probably benign	0.14
R0304:Vmn1r86	UTSW	7	12,836,707 (GRCm39)	missense	probably benign
R0525:Vmn1r86	UTSW	7	12,836,088 (GRCm39)	missense	probably benign	0.13
R1958:Vmn1r86	UTSW	7	12,836,621 (GRCm39)	missense	possibly damaging	0.94
R3149:Vmn1r86	UTSW	7	12,836,358 (GRCm39)	nonsense	probably null
R3896:Vmn1r86	UTSW	7	12,836,093 (GRCm39)	missense	probably benign	0.18
R4735:Vmn1r86	UTSW	7	12,836,221 (GRCm39)	missense	probably damaging	1.00
R5219:Vmn1r86	UTSW	7	12,836,382 (GRCm39)	missense	probably damaging	1.00
R6003:Vmn1r86	UTSW	7	12,836,125 (GRCm39)	nonsense	probably null
R6457:Vmn1r86	UTSW	7	12,836,279 (GRCm39)	missense	possibly damaging	0.70
R7442:Vmn1r86	UTSW	7	12,835,983 (GRCm39)	missense	possibly damaging	0.70
R7622:Vmn1r86	UTSW	7	12,836,685 (GRCm39)	missense	probably benign	0.07
R8436:Vmn1r86	UTSW	7	12,836,771 (GRCm39)	missense	probably benign	0.25
R9141:Vmn1r86	UTSW	7	12,836,789 (GRCm39)	nonsense	probably null
R9374:Vmn1r86	UTSW	7	12,836,781 (GRCm39)	missense	possibly damaging	0.86
R9398:Vmn1r86	UTSW	7	12,836,261 (GRCm39)	missense	probably damaging	0.98
R9551:Vmn1r86	UTSW	7	12,836,781 (GRCm39)	missense	possibly damaging	0.86
R9552:Vmn1r86	UTSW	7	12,836,781 (GRCm39)	missense	possibly damaging	0.86
R9606:Vmn1r86	UTSW	7	12,836,741 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGGTGCAGATGGACAGACC -3'
(R):5'- AGGAAAGAGTTGAACATTTCATCAC -3'

Sequencing Primer
(F):5'- CGGCTGACTCTGAATGAAAAC -3'
(R):5'- AGAGTTGAACATTTCATCACTTCTC -3'

Posted On

2014-10-15