Mutagenetix > Incidental Mutation

Incidental Mutation 'R2230:Mrgprb3'

239984

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb3

Ensembl Gene

ENSMUSG00000070546

Gene Name

MAS-related GPR, member B3

Synonyms

MrgB3

MMRRC Submission

040231-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R2230 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

48292611-48293549 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 48292770 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 260 (I260M)

Ref Sequence

ENSEMBL: ENSMUSP00000091945 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094383]

AlphaFold

Q91ZC1

Predicted Effect

probably benign
Transcript: ENSMUST00000094383
AA Change: I260M

PolyPhen 2 Score 0.049 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000091945
Gene: ENSMUSG00000070546
AA Change: I260M

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	25	279	4e-13	SMART
low complexity region	301	312	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

100% (62/62)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam12	A	T	7: 133,521,347 (GRCm39)	N280K	probably damaging	Het
Adgrf4	C	T	17: 42,977,789 (GRCm39)	R518Q	possibly damaging	Het
Ankra2	T	C	13: 98,407,646 (GRCm39)	F199L	probably damaging	Het
Ankrd63	A	G	2: 118,533,846 (GRCm39)		probably benign	Het
Apoa2	A	G	1: 171,053,340 (GRCm39)	K53R	probably benign	Het
Atr	A	G	9: 95,802,818 (GRCm39)	R1827G	probably damaging	Het
Ccdc61	T	C	7: 18,625,032 (GRCm39)	E502G	probably damaging	Het
Ccn2	T	A	10: 24,472,371 (GRCm39)	M138K	possibly damaging	Het
Cdon	T	C	9: 35,403,222 (GRCm39)		probably null	Het
Cyp2c68	T	A	19: 39,687,804 (GRCm39)	S398C	probably benign	Het
Cyp2e1	T	C	7: 140,344,827 (GRCm39)	S98P	probably damaging	Het
Dock2	T	A	11: 34,244,323 (GRCm39)	I1036F	probably damaging	Het
Entpd7	T	C	19: 43,710,255 (GRCm39)	V304A	probably benign	Het
Entrep2	G	A	7: 64,408,970 (GRCm39)	H475Y	probably damaging	Het
Ergic3	A	G	2: 155,859,736 (GRCm39)	T346A	probably damaging	Het
F2	T	C	2: 91,456,102 (GRCm39)	D553G	probably benign	Het
Fam227a	T	A	15: 79,499,582 (GRCm39)	Y591F	possibly damaging	Het
Gal3st1	T	C	11: 3,948,282 (GRCm39)	I163T	probably benign	Het
Gm10650	A	G	3: 127,833,412 (GRCm39)		noncoding transcript	Het
Gm21850	G	T	2: 153,900,248 (GRCm39)	V202L	probably benign	Het
Hdc	T	A	2: 126,435,938 (GRCm39)	E644D	possibly damaging	Het
Hypk	G	A	2: 121,287,773 (GRCm39)		probably null	Het
Kif21a	G	A	15: 90,869,565 (GRCm39)	Q429*	probably null	Het
Mgll	G	A	6: 88,802,714 (GRCm39)	V318M	possibly damaging	Het
Musk	A	T	4: 58,333,672 (GRCm39)	I256F	possibly damaging	Het
Myl3	T	C	9: 110,596,979 (GRCm39)	L113P	probably damaging	Het
Myo5c	A	G	9: 75,180,888 (GRCm39)	D759G	probably benign	Het
Nkx2-1	G	A	12: 56,580,071 (GRCm39)	Q290*	probably null	Het
Oaz3	T	C	3: 94,341,846 (GRCm39)	T130A	probably benign	Het
Or10j7	A	G	1: 173,011,182 (GRCm39)	I273T	probably benign	Het
Or4c3d	A	T	2: 89,882,569 (GRCm39)	F33Y	probably benign	Het
Or7g16	A	G	9: 18,727,021 (GRCm39)	S190P	probably damaging	Het
Or8c13	T	C	9: 38,091,442 (GRCm39)	T226A	probably benign	Het
Pabpc2	G	A	18: 39,908,123 (GRCm39)	V463I	probably benign	Het
Piezo2	A	T	18: 63,278,143 (GRCm39)	C254S	probably damaging	Het
Plxnd1	C	A	6: 115,941,105 (GRCm39)	R1302L	probably damaging	Het
Pnpla7	T	C	2: 24,941,610 (GRCm39)		probably benign	Het
Ppl	G	A	16: 4,906,845 (GRCm39)	T1150I	possibly damaging	Het
Prkag2	G	T	5: 25,113,362 (GRCm39)	A113E	probably benign	Het
Proz	A	G	8: 13,113,356 (GRCm39)	Y59C	probably damaging	Het
Prr5	T	C	15: 84,586,981 (GRCm39)	S244P	probably benign	Het
Sec14l5	A	G	16: 4,994,345 (GRCm39)	T380A	probably damaging	Het
Snw1	A	G	12: 87,499,428 (GRCm39)	V391A	probably benign	Het
Sp2	C	T	11: 96,846,762 (GRCm39)	C527Y	probably damaging	Het
Sspo	A	G	6: 48,425,606 (GRCm39)	I76V	probably damaging	Het
Sspo	C	A	6: 48,477,437 (GRCm39)	Q5123K	probably benign	Het
Tbc1d21	A	C	9: 58,270,363 (GRCm39)	N137K	probably damaging	Het
Tek	G	A	4: 94,699,573 (GRCm39)	C317Y	probably damaging	Het
Tet3	T	C	6: 83,346,453 (GRCm39)	D1328G	probably damaging	Het
Topbp1	T	A	9: 103,223,047 (GRCm39)	I1377N	probably damaging	Het
Trmt9b	T	A	8: 36,979,707 (GRCm39)	C437S	probably damaging	Het
Ttf2	A	G	3: 100,865,260 (GRCm39)	V544A	probably damaging	Het
Ttn	A	T	2: 76,774,497 (GRCm39)	F2136L	probably damaging	Het
Ugt2b38	A	G	5: 87,569,527 (GRCm39)	F267L	probably benign	Het
Usb1	T	G	8: 96,070,674 (GRCm39)	L200R	probably damaging	Het
Vwa5a	G	A	9: 38,645,174 (GRCm39)	G420R	probably null	Het
Vwa8	T	C	14: 79,329,843 (GRCm39)		probably null	Het
Zfp708	A	T	13: 67,219,036 (GRCm39)	Y229*	probably null	Het
Zzef1	T	A	11: 72,775,242 (GRCm39)	M1745K	probably damaging	Het

Other mutations in Mrgprb3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03069:Mrgprb3	APN	7	48,293,198 (GRCm39)	missense	possibly damaging	0.71
Reserve	UTSW	7	48,293,447 (GRCm39)	missense	probably benign	0.02
starker	UTSW	7	48,293,116 (GRCm39)	missense	probably benign	0.28
IGL03052:Mrgprb3	UTSW	7	48,293,341 (GRCm39)	missense	possibly damaging	0.93
R0446:Mrgprb3	UTSW	7	48,292,984 (GRCm39)	missense	probably benign	0.42
R0546:Mrgprb3	UTSW	7	48,293,263 (GRCm39)	missense	probably damaging	1.00
R0885:Mrgprb3	UTSW	7	48,292,844 (GRCm39)	missense	probably damaging	1.00
R1764:Mrgprb3	UTSW	7	48,292,771 (GRCm39)	missense	probably benign	0.01
R2044:Mrgprb3	UTSW	7	48,293,482 (GRCm39)	missense	possibly damaging	0.92
R2232:Mrgprb3	UTSW	7	48,292,770 (GRCm39)	missense	probably benign	0.05
R2240:Mrgprb3	UTSW	7	48,293,389 (GRCm39)	missense	probably damaging	0.99
R3001:Mrgprb3	UTSW	7	48,293,232 (GRCm39)	missense	probably benign
R3002:Mrgprb3	UTSW	7	48,293,232 (GRCm39)	missense	probably benign
R4717:Mrgprb3	UTSW	7	48,293,000 (GRCm39)	missense	probably benign	0.01
R4805:Mrgprb3	UTSW	7	48,293,054 (GRCm39)	missense	probably benign	0.01
R5083:Mrgprb3	UTSW	7	48,292,762 (GRCm39)	missense	probably benign	0.01
R5311:Mrgprb3	UTSW	7	48,293,059 (GRCm39)	missense	probably damaging	1.00
R5330:Mrgprb3	UTSW	7	48,292,682 (GRCm39)	missense	possibly damaging	0.90
R5331:Mrgprb3	UTSW	7	48,292,682 (GRCm39)	missense	possibly damaging	0.90
R5615:Mrgprb3	UTSW	7	48,293,234 (GRCm39)	missense	probably benign	0.01
R5621:Mrgprb3	UTSW	7	48,293,116 (GRCm39)	missense	probably benign	0.28
R5697:Mrgprb3	UTSW	7	48,292,673 (GRCm39)	missense	probably damaging	0.96
R5725:Mrgprb3	UTSW	7	48,293,548 (GRCm39)	start codon destroyed	probably null	0.02
R5758:Mrgprb3	UTSW	7	48,293,067 (GRCm39)	missense	probably benign	0.01
R5807:Mrgprb3	UTSW	7	48,293,110 (GRCm39)	missense	probably benign	0.02
R5908:Mrgprb3	UTSW	7	48,293,366 (GRCm39)	missense	probably damaging	0.98
R6902:Mrgprb3	UTSW	7	48,293,447 (GRCm39)	missense	probably benign	0.02
R7037:Mrgprb3	UTSW	7	48,292,942 (GRCm39)	missense	probably damaging	1.00
R7288:Mrgprb3	UTSW	7	48,293,059 (GRCm39)	missense	probably damaging	1.00
R7605:Mrgprb3	UTSW	7	48,292,862 (GRCm39)	missense	probably benign	0.01
R8246:Mrgprb3	UTSW	7	48,293,268 (GRCm39)	missense	probably benign	0.02
R8292:Mrgprb3	UTSW	7	48,293,255 (GRCm39)	missense	probably benign	0.01
R8972:Mrgprb3	UTSW	7	48,293,422 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TCGGTTGCTGCACTAGACTG -3'
(R):5'- TCTGTGGGTTCAGCCTCATC -3'

Sequencing Primer
(F):5'- GCTGCACTAGACTGTTCCCAG -3'
(R):5'- GGGTTCAGCCTCATCCTGCTC -3'

Posted On

2014-10-15