Mutagenetix > Incidental Mutations

Incidental Mutation 'R2233:Olfr1154'

240156

Institutional Source

Beutler Lab

Gene Symbol

Olfr1154

Ensembl Gene

ENSMUSG00000075146

Gene Name

olfactory receptor 1154

Synonyms

GA_x6K02T2Q125-49403456-49402524, MOR173-2

MMRRC Submission

040234-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.089) question?

Stock #

R2233 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

87901256-87905166 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 87903475 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Phenylalanine at position 67 (S67F)

Ref Sequence

ENSEMBL: ENSMUSP00000148909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099844] [ENSMUST00000215017] [ENSMUST00000215862]

Predicted Effect

probably damaging
Transcript: ENSMUST00000099844
AA Change: S67F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097432
Gene: ENSMUSG00000075146
AA Change: S67F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	8.1e-47	PFAM
Pfam:7tm_1	41	289	3.6e-18	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215017
AA Change: S67F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215862
AA Change: S67F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam23	A	G	1: 63,545,512	I360V	probably benign	Het
Adarb1	A	G	10: 77,317,349	V322A	probably damaging	Het
Atad2b	T	G	12: 5,006,745	F867C	probably damaging	Het
Ccdc24	T	C	4: 117,869,916	K79R	possibly damaging	Het
Cfap44	A	G	16: 44,451,525	I1214V	probably benign	Het
Chrm4	A	G	2: 91,928,530	S428G	probably benign	Het
Chrnb1	A	T	11: 69,795,602	I64N	probably damaging	Het
Crh	A	T	3: 19,693,932	M182K	probably damaging	Het
Dazap1	A	G	10: 80,277,599	K110E	possibly damaging	Het
Dhx16	T	C	17: 35,887,886	C737R	probably damaging	Het
Dst	A	G	1: 34,274,262	E6384G	probably damaging	Het
Dync1i2	C	T	2: 71,249,420	Q419*	probably null	Het
E2f4	T	A	8: 105,298,651	V121E	probably damaging	Het
Enah	G	A	1: 181,921,972	P415L	probably damaging	Het
Fat3	G	A	9: 15,998,271	S2145F	probably damaging	Het
Fezf1	T	C	6: 23,246,003	T388A	probably damaging	Het
Gimap6	T	A	6: 48,704,484	H72L	possibly damaging	Het
Gm11149	A	G	9: 49,562,146		probably benign	Het
Gm12695	C	T	4: 96,724,029	R499Q	probably damaging	Het
Grhl1	A	G	12: 24,608,511	D385G	probably damaging	Het
Hyou1	C	T	9: 44,389,091	T855M	probably benign	Het
Igf1r	T	A	7: 68,212,080	N1129K	probably damaging	Het
Iglon5	T	C	7: 43,480,638	E34G	probably damaging	Het
Kcnab1	T	A	3: 65,319,467	V189D	probably damaging	Het
Kifap3	T	A	1: 163,856,065	D438E	probably benign	Het
Lmo2	T	C	2: 103,981,062	Y147H	probably damaging	Het
Lrrc41	G	A	4: 116,096,385	R756Q	possibly damaging	Het
Lrrc49	A	T	9: 60,598,157	F538L	possibly damaging	Het
Nphp3	G	A	9: 104,037,376	R1052H	probably benign	Het
Nynrin	G	A	14: 55,872,067	V1544I	possibly damaging	Het
Obscn	T	C	11: 59,131,646	R758G	possibly damaging	Het
Olfr1346	T	C	7: 6,474,442	S111P	possibly damaging	Het
Osbpl6	T	C	2: 76,586,769	F577L	probably damaging	Het
Otc	A	G	X: 10,303,367	Q216R	probably benign	Het
Ppp1r12a	A	G	10: 108,198,919	I108M	possibly damaging	Het
Prl7a1	C	A	13: 27,642,419		probably null	Het
Prss16	T	C	13: 22,009,409	D72G	possibly damaging	Het
Qrsl1	T	C	10: 43,896,096	K33E	probably benign	Het
Rabepk	A	T	2: 34,795,234	I58N	possibly damaging	Het
Ralgapa1	A	T	12: 55,717,071	H1403Q	probably benign	Het
Rhobtb3	C	A	13: 75,872,365	C606F	possibly damaging	Het
Rnf216	G	A	5: 143,090,926	H68Y	probably benign	Het
Scap	T	C	9: 110,381,593	C998R	probably damaging	Het
Scgb1b27	T	C	7: 34,021,824	Y46H	probably damaging	Het
Sel1l2	T	C	2: 140,244,165	Y502C	probably damaging	Het
Sf3a2	G	T	10: 80,802,829	A95S	probably benign	Het
Sis	A	T	3: 72,913,194	F1412L	probably benign	Het
Slc25a29	A	T	12: 108,835,661	C9S	possibly damaging	Het
Snap91	T	C	9: 86,798,571	T427A	probably benign	Het
St3gal6	A	G	16: 58,473,534	F211L	probably damaging	Het
Stab1	G	A	14: 31,161,880	S240F	probably benign	Het
Sun3	T	A	11: 9,023,371	K109*	probably null	Het
Syne1	T	C	10: 5,041,484	N8410S	probably benign	Het
Tbx18	G	T	9: 87,724,350	S247R	probably damaging	Het
Tenm3	T	C	8: 48,276,169	I1601V	probably benign	Het
Tmem135	T	C	7: 89,154,074	N297S	probably damaging	Het
Tnk1	C	T	11: 69,855,191		probably null	Het
Txnl4b	A	G	8: 109,568,919		probably benign	Het
Uck1	T	C	2: 32,258,303	D167G	probably damaging	Het
Vmn2r124	A	T	17: 18,049,665	H61L	possibly damaging	Het
Xylt2	C	T	11: 94,669,996	V239M	possibly damaging	Het
Zmpste24	T	C	4: 121,097,965	D12G	probably benign	Het
Zscan25	A	G	5: 145,283,692	Y99C	probably damaging	Het

Other mutations in Olfr1154

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Olfr1154	APN	2	87903527	missense	probably benign	0.00
IGL01878:Olfr1154	APN	2	87903331	nonsense	probably null
IGL02683:Olfr1154	APN	2	87903104	missense	possibly damaging	0.80
IGL02836:Olfr1154	APN	2	87903380	missense	possibly damaging	0.81
R0432:Olfr1154	UTSW	2	87902960	missense	probably damaging	1.00
R1123:Olfr1154	UTSW	2	87902904	missense	probably damaging	0.99
R1223:Olfr1154	UTSW	2	87902819	missense	probably damaging	0.99
R1561:Olfr1154	UTSW	2	87903161	missense	probably benign	0.00
R1964:Olfr1154	UTSW	2	87903667	missense	probably benign	0.00
R2041:Olfr1154	UTSW	2	87902797	missense	probably damaging	1.00
R2219:Olfr1154	UTSW	2	87902925	nonsense	probably null
R3719:Olfr1154	UTSW	2	87903103	missense	probably benign	0.05
R4826:Olfr1154	UTSW	2	87903349	missense	probably damaging	1.00
R4908:Olfr1154	UTSW	2	87903189	missense	probably damaging	1.00
R5056:Olfr1154	UTSW	2	87903571	missense	probably damaging	1.00
R5589:Olfr1154	UTSW	2	87903347	missense	probably benign	0.26
R6477:Olfr1154	UTSW	2	87902990	missense	probably damaging	1.00
R6532:Olfr1154	UTSW	2	87903202	missense	probably damaging	1.00
R6666:Olfr1154	UTSW	2	87903508	missense	probably damaging	1.00
R6693:Olfr1154	UTSW	2	87903308	missense	probably damaging	1.00
R6724:Olfr1154	UTSW	2	87903602	missense	probably benign	0.00
R7784:Olfr1154	UTSW	2	87903193	missense	probably benign	0.01
Z1088:Olfr1154	UTSW	2	87903584	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TGCTCCAAATGCCAGGTGAG -3'
(R):5'- AAGACTGAATTCTATCTCCTGGG -3'

Sequencing Primer
(F):5'- CTCCAAATGCCAGGTGAGTACAG -3'
(R):5'- GGATTTAGTGATCATCCAGAGCTCC -3'

Posted On

2014-10-15