|Institutional Source||Beutler Lab|
|Gene Name||polymerase (RNA) II (DNA directed) polypeptide A|
|Is this an essential gene?||Probably essential (E-score: 0.965)|
|Stock #||R2217 (G1)|
|Chromosomal Location||69733997-69758637 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||A to G at 69742685 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000071200 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000058470] [ENSMUST00000071213]|
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a reporter allele show prenatal lethality. Homozygotes for a small deletion in the C-terminal domain are viable, fertile and developmentally normal. Homozygotes for a larger deletion show reduced fetal size and partial postnatal lethality; survivors are small but otherwise normal. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Polr2a||
(F):5'- GGAGTTCCTCACAGTTGCATC -3'
(R):5'- TGTCGGGCAGCAGAATGTAG -3'
(F):5'- ACAGTTGCATCATATTTCACCATCAC -3'
(R):5'- TCCCATTTGGATTCAAGCATCGG -3'