Mutagenetix > Incidental Mutation

Incidental Mutation 'R2217:Gm7535'

241233

Institutional Source

Beutler Lab

Gene Symbol

Gm7535

Ensembl Gene

ENSMUSG00000090957

Gene Name

predicted gene 7535

Synonyms

MMRRC Submission

040219-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.197) question?

Stock #

R2217 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

18131304-18132987 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to G at 18131936 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000093316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054871] [ENSMUST00000164167]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000054871

SMART Domains

Protein: ENSMUSP00000093316
Gene: ENSMUSG00000079700

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	302	2.2e-43	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000164167

SMART Domains

Protein: ENSMUSP00000125897
Gene: ENSMUSG00000090957

Domain	Start	End	E-Value	Type
Pfam:CNDH2_C	15	292	6.7e-90	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000231820

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adat1	T	C	8: 112,709,128 (GRCm39)	K232E	probably benign	Het
Apba1	T	C	19: 23,871,326 (GRCm39)	M386T	probably damaging	Het
Appl2	G	T	10: 83,444,601 (GRCm39)	F472L	possibly damaging	Het
Atxn2	T	C	5: 121,941,140 (GRCm39)	Y56H	probably damaging	Het
Cacna1c	T	C	6: 118,647,368 (GRCm39)	Y809C	probably damaging	Het
Canx	C	T	11: 50,201,694 (GRCm39)	V59I	probably benign	Het
Catsperb	T	C	12: 101,560,478 (GRCm39)	L823P	probably damaging	Het
Crb3	T	C	17: 57,372,090 (GRCm39)	S46P	probably benign	Het
Daam1	G	A	12: 72,036,601 (GRCm39)	R1058H	probably damaging	Het
Ehd1	T	A	19: 6,348,502 (GRCm39)	D493E	probably damaging	Het
Eml6	T	A	11: 29,768,907 (GRCm39)	Q746L	probably damaging	Het
Epg5	T	A	18: 77,992,287 (GRCm39)	M328K	probably benign	Het
Flt4	T	A	11: 49,515,555 (GRCm39)	S48T	probably benign	Het
Gm10608	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	9: 118,989,784 (GRCm39)		probably null	Het
Gpat4	G	A	8: 23,670,171 (GRCm39)	P286L	probably damaging	Het
Hmcn2	A	T	2: 31,240,586 (GRCm39)	T494S	probably benign	Het
Hydin	G	A	8: 111,145,138 (GRCm39)	V830I	probably benign	Het
Map3k6	T	A	4: 132,973,983 (GRCm39)	H487Q	possibly damaging	Het
Myh14	G	A	7: 44,283,800 (GRCm39)	P735S	probably damaging	Het
Nfkb2	T	C	19: 46,296,163 (GRCm39)		probably null	Het
Nlrp4c	T	C	7: 6,076,113 (GRCm39)	V671A	probably benign	Het
Nsrp1	A	T	11: 76,936,587 (GRCm39)	Y536*	probably null	Het
Or4c112	G	T	2: 88,853,770 (GRCm39)	H192Q	probably benign	Het
Pak5	A	G	2: 135,958,123 (GRCm39)	S322P	probably damaging	Het
Pank2	A	G	2: 131,124,601 (GRCm39)		probably null	Het
Phf6	A	T	X: 52,031,525 (GRCm39)	I272F	probably damaging	Het
Plxna2	T	C	1: 194,480,056 (GRCm39)	L1409P	probably damaging	Het
Polr2a	A	G	11: 69,633,511 (GRCm39)		probably null	Het
Pp2d1	C	A	17: 53,822,482 (GRCm39)	V195L	probably benign	Het
Psma1	C	T	7: 113,864,173 (GRCm39)	E227K	unknown	Het
Ptger1	C	T	8: 84,395,357 (GRCm39)	T278I	probably benign	Het
Slc20a2	C	A	8: 23,050,532 (GRCm39)	S250R	probably benign	Het
Slc27a2	A	G	2: 126,409,672 (GRCm39)	T285A	probably damaging	Het
Slc47a2	T	C	11: 61,204,497 (GRCm39)	T285A	probably benign	Het
Slf1	T	A	13: 77,194,825 (GRCm39)		probably null	Het
Tiam2	A	G	17: 3,465,389 (GRCm39)	T373A	probably benign	Het
Timd2	T	A	11: 46,577,844 (GRCm39)	I96L	probably damaging	Het
Tmem59l	A	G	8: 70,939,951 (GRCm39)	L6S	unknown	Het
Tmem64	T	C	4: 15,266,658 (GRCm39)	I236T	possibly damaging	Het
Trpm2	T	A	10: 77,777,016 (GRCm39)	D427V	probably damaging	Het
Vax2	A	T	6: 83,714,871 (GRCm39)	Y262F	probably damaging	Het
Virma	T	C	4: 11,544,924 (GRCm39)	S1628P	probably damaging	Het
Zan	C	A	5: 137,408,568 (GRCm39)		probably benign	Het
Zbtb22	T	G	17: 34,136,939 (GRCm39)	D361E	probably damaging	Het
Zfp27	A	G	7: 29,595,536 (GRCm39)	L143P	possibly damaging	Het

Other mutations in Gm7535

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL00422:Gm7535	APN	17	18,132,150 (GRCm39)	intron	probably benign
IGL01663:Gm7535	APN	17	18,131,619 (GRCm39)	intron	probably benign
R0165:Gm7535	UTSW	17	18,131,437 (GRCm39)	intron	probably benign
R0335:Gm7535	UTSW	17	18,131,374 (GRCm39)	intron	probably benign
R1985:Gm7535	UTSW	17	18,131,800 (GRCm39)	intron	probably benign
R2218:Gm7535	UTSW	17	18,131,936 (GRCm39)	intron	probably benign
R4464:Gm7535	UTSW	17	18,131,924 (GRCm39)	intron	probably benign
R4581:Gm7535	UTSW	17	18,131,345 (GRCm39)	intron	probably benign
R4887:Gm7535	UTSW	17	18,131,333 (GRCm39)	intron	probably benign
R5225:Gm7535	UTSW	17	18,131,809 (GRCm39)	intron	probably benign
R5305:Gm7535	UTSW	17	18,132,061 (GRCm39)	intron	probably benign
R5641:Gm7535	UTSW	17	18,131,788 (GRCm39)	intron	probably benign
R5658:Gm7535	UTSW	17	18,131,582 (GRCm39)	intron	probably benign
R5760:Gm7535	UTSW	17	18,132,080 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- AGCTCCTTGTATCTCATGGC -3'
(R):5'- AAACCCTATTCTGTGCCACCTG -3'

Sequencing Primer
(F):5'- TCTGCATCAAGGTCAGCAG -3'
(R):5'- CTATTCTGTGCCACCTGGTGTG -3'

Posted On

2014-10-15