Mutagenetix > Incidental Mutation

Incidental Mutation 'R2224:Fpr3'

241575

Institutional Source

Beutler Lab

Gene Symbol

Fpr3

Ensembl Gene

ENSMUSG00000079700

Gene Name

formyl peptide receptor 3

Synonyms

Fprl1, LXA4-R, Lxa4r, Fpr-rs1

MMRRC Submission

040225-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2224 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

18190720-18191939 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 18191455 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 242 (V242D)

Ref Sequence

ENSEMBL: ENSMUSP00000111227 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054871] [ENSMUST00000115565]

AlphaFold

O08790

Predicted Effect

probably damaging
Transcript: ENSMUST00000054871
AA Change: V242D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000093316
Gene: ENSMUSG00000079700
AA Change: V242D

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	302	2.2e-43	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000115565
AA Change: V242D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000111227
Gene: ENSMUSG00000079700
AA Change: V242D

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	302	1.5e-36	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	T	G	16: 14,289,932 (GRCm39)	L1416R	probably damaging	Het
Aen	C	A	7: 78,552,199 (GRCm39)	T15K	probably benign	Het
Ahnak	G	A	19: 8,990,355 (GRCm39)		probably benign	Het
Ar	T	A	X: 97,194,937 (GRCm39)	M517K	probably benign	Het
Arsb	T	A	13: 93,930,679 (GRCm39)	F216I	probably damaging	Het
Atg9b	A	G	5: 24,591,393 (GRCm39)	V735A	possibly damaging	Het
Brinp3	C	T	1: 146,777,658 (GRCm39)	Q702*	probably null	Het
Btbd9	C	A	17: 30,746,320 (GRCm39)	A169S	probably damaging	Het
Cacna1h	T	C	17: 25,604,917 (GRCm39)	N1132S	probably benign	Het
Ccdc33	A	G	9: 57,989,305 (GRCm39)	S123P	probably damaging	Het
Cep250	A	C	2: 155,833,737 (GRCm39)	E1886D	possibly damaging	Het
Chd9	A	C	8: 91,737,913 (GRCm39)	H1515P	probably benign	Het
Cyp2c68	A	G	19: 39,724,026 (GRCm39)	C213R	probably benign	Het
Ddx41	G	A	13: 55,679,214 (GRCm39)	T544I	probably damaging	Het
Dgkk	T	A	X: 6,741,487 (GRCm39)	D102E	probably damaging	Het
Eea1	T	A	10: 95,855,874 (GRCm39)	V637D	probably damaging	Het
Emcn	T	C	3: 137,109,778 (GRCm39)	I140T	possibly damaging	Het
Exo1	A	G	1: 175,714,254 (GRCm39)		probably null	Het
Fbxo21	T	G	5: 118,146,188 (GRCm39)	Y597D	probably damaging	Het
Fbxw20	G	C	9: 109,062,650 (GRCm39)	Q59E	possibly damaging	Het
Fyb1	A	G	15: 6,681,864 (GRCm39)	Y737C	probably damaging	Het
Gm17661	GG	GGG	2: 90,748,052 (GRCm39)		noncoding transcript	Het
Hdac9	C	T	12: 34,457,801 (GRCm39)	V251I	probably benign	Het
Hoxa10	T	C	6: 52,209,616 (GRCm39)	E52G	probably damaging	Het
Il17f	A	G	1: 20,849,599 (GRCm39)	V55A	probably damaging	Het
Inpp1	T	A	1: 52,829,290 (GRCm39)	E243V	probably benign	Het
Leap2	A	G	11: 53,313,634 (GRCm39)	L46P	probably damaging	Het
Map4k3	A	T	17: 80,937,883 (GRCm39)	D339E	probably benign	Het
Met	A	G	6: 17,563,721 (GRCm39)		probably null	Het
Mgam	G	T	6: 40,741,208 (GRCm39)		probably null	Het
Mrgpra1	A	G	7: 46,984,854 (GRCm39)	V275A	possibly damaging	Het
Mrgprx2	T	A	7: 48,132,608 (GRCm39)	Q70L	probably benign	Het
Mtus1	C	T	8: 41,535,812 (GRCm39)	V635M	probably damaging	Het
Mup18	G	T	4: 61,590,128 (GRCm39)	F133L	possibly damaging	Het
Naalad2	A	T	9: 18,287,829 (GRCm39)	V267E	possibly damaging	Het
Nin	A	T	12: 70,108,004 (GRCm39)	I196N	probably damaging	Het
Nkpd1	A	T	7: 19,253,745 (GRCm39)	Y37F	probably benign	Het
Odc1	A	G	12: 17,597,336 (GRCm39)	I13V	probably benign	Het
Or1ad1	T	C	11: 50,876,059 (GRCm39)	F177S	probably damaging	Het
Or4a67	A	T	2: 88,597,930 (GRCm39)	M243K	possibly damaging	Het
Or5m12	T	A	2: 85,735,099 (GRCm39)	I100F	probably benign	Het
Pard3	A	G	8: 128,086,257 (GRCm39)	I286V	probably damaging	Het
Pcx	T	C	19: 4,668,026 (GRCm39)	I516T	possibly damaging	Het
Ppp1r9a	G	T	6: 5,154,074 (GRCm39)	R1081L	probably benign	Het
Prpf40b	C	T	15: 99,201,172 (GRCm39)		probably benign	Het
Rnmt	C	T	18: 68,438,854 (GRCm39)		probably benign	Het
Saxo5	A	G	8: 3,529,249 (GRCm39)	T245A	probably benign	Het
Sfxn1	A	G	13: 54,239,536 (GRCm39)	T20A	possibly damaging	Het
Sirt2	A	G	7: 28,471,637 (GRCm39)		probably null	Het
Spata31d1a	T	A	13: 59,851,529 (GRCm39)	I200L	probably benign	Het
Spin2g	A	T	X: 33,656,599 (GRCm39)	I171N	possibly damaging	Het
Tesl2	T	A	X: 23,825,173 (GRCm39)	M1L	probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Tsbp1	C	A	17: 34,667,737 (GRCm39)		probably null	Het
Uqcrc2	T	A	7: 120,240,937 (GRCm39)	V73E	probably damaging	Het
Vmn2r100	A	G	17: 19,742,634 (GRCm39)	K336R	probably benign	Het
Vmn2r108	A	T	17: 20,701,295 (GRCm39)	Y68*	probably null	Het
Zbtb4	C	T	11: 69,667,184 (GRCm39)	T163I	probably benign	Het

Other mutations in Fpr3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Fpr3	APN	17	18,190,828 (GRCm39)	missense	probably benign	0.17
IGL01520:Fpr3	APN	17	18,191,325 (GRCm39)	missense	possibly damaging	0.69
IGL02166:Fpr3	APN	17	18,190,726 (GRCm39)	utr 5 prime	probably benign
IGL02380:Fpr3	APN	17	18,191,254 (GRCm39)	missense	probably benign	0.00
IGL02587:Fpr3	APN	17	18,190,953 (GRCm39)	missense	probably benign	0.12
R1521:Fpr3	UTSW	17	18,191,277 (GRCm39)	missense	probably damaging	1.00
R1533:Fpr3	UTSW	17	18,190,922 (GRCm39)	nonsense	probably null
R1913:Fpr3	UTSW	17	18,191,670 (GRCm39)	missense	probably damaging	0.96
R2099:Fpr3	UTSW	17	18,191,443 (GRCm39)	missense	probably damaging	1.00
R2140:Fpr3	UTSW	17	18,190,879 (GRCm39)	missense	probably damaging	1.00
R2206:Fpr3	UTSW	17	18,190,908 (GRCm39)	missense	probably damaging	1.00
R2219:Fpr3	UTSW	17	18,191,644 (GRCm39)	missense	possibly damaging	0.93
R2244:Fpr3	UTSW	17	18,191,449 (GRCm39)	missense	probably benign	0.03
R2994:Fpr3	UTSW	17	18,191,130 (GRCm39)	nonsense	probably null
R5364:Fpr3	UTSW	17	18,190,806 (GRCm39)	missense	probably benign	0.00
R6179:Fpr3	UTSW	17	18,190,919 (GRCm39)	nonsense	probably null
R6781:Fpr3	UTSW	17	18,190,978 (GRCm39)	missense	probably benign	0.09
R6909:Fpr3	UTSW	17	18,191,429 (GRCm39)	missense	probably benign	0.00
R7565:Fpr3	UTSW	17	18,191,227 (GRCm39)	missense	probably damaging	1.00
R8008:Fpr3	UTSW	17	18,191,715 (GRCm39)	missense	probably benign	0.03
R8097:Fpr3	UTSW	17	18,191,054 (GRCm39)	missense	probably damaging	1.00
R8351:Fpr3	UTSW	17	18,191,436 (GRCm39)	missense	probably benign	0.27
R8732:Fpr3	UTSW	17	18,191,223 (GRCm39)	missense	possibly damaging	0.94
R8994:Fpr3	UTSW	17	18,191,341 (GRCm39)	missense	possibly damaging	0.92
R9076:Fpr3	UTSW	17	18,191,725 (GRCm39)	missense	probably benign
R9206:Fpr3	UTSW	17	18,191,131 (GRCm39)	missense	probably damaging	1.00
R9208:Fpr3	UTSW	17	18,191,131 (GRCm39)	missense	probably damaging	1.00
R9327:Fpr3	UTSW	17	18,191,713 (GRCm39)	missense	probably damaging	1.00
R9553:Fpr3	UTSW	17	18,191,612 (GRCm39)	missense	probably damaging	1.00
X0021:Fpr3	UTSW	17	18,191,500 (GRCm39)	missense	probably benign	0.06
Z1176:Fpr3	UTSW	17	18,191,255 (GRCm39)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- TTTGTATCCTGGGGCAACTCTG -3'
(R):5'- AGTCTTTCTTGAAAGTCCTGGCC -3'

Sequencing Primer
(F):5'- CTCTGTTGAGGAAAGGTTGAACAC -3'
(R):5'- GTCCTGGCCCATGAAAACATAGAG -3'

Posted On

2014-10-15