Mutagenetix > Incidental Mutation

Incidental Mutation 'R2281:Mga'

243098

Institutional Source

Beutler Lab

Gene Symbol

Mga

Ensembl Gene

ENSMUSG00000033943

Gene Name

MAX gene associated

Synonyms

D030062C11Rik, Mga, Mad5, C130042M01Rik

MMRRC Submission

040280-MU

Accession Numbers

Ncbi RefSeq: NM_013720.2, NM_001164274.1; MGI: 1352483

Essential gene?

Probably essential (E-score: 0.955) question?

Stock #

R2281 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

119897228-119969581 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 119903723 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 351 (I351V)

Ref Sequence

ENSEMBL: ENSMUSP00000119044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046717] [ENSMUST00000079934] [ENSMUST00000110773] [ENSMUST00000110774] [ENSMUST00000156510]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000046717
AA Change: I351V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000043795
Gene: ENSMUSG00000033943
AA Change: I351V

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	6e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1248	1269	N/A	INTRINSIC
low complexity region	1301	1315	N/A	INTRINSIC
low complexity region	1564	1581	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC
low complexity region	1681	1716	N/A	INTRINSIC
low complexity region	1796	1818	N/A	INTRINSIC
low complexity region	1833	1850	N/A	INTRINSIC
low complexity region	1977	1992	N/A	INTRINSIC
low complexity region	2183	2197	N/A	INTRINSIC
low complexity region	2241	2259	N/A	INTRINSIC
HLH	2368	2419	8.27e-7	SMART
low complexity region	2748	2769	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000079934
AA Change: I351V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000078853
Gene: ENSMUSG00000033943
AA Change: I351V

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	5e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1300	1314	N/A	INTRINSIC
low complexity region	1626	1648	N/A	INTRINSIC
low complexity region	1663	1680	N/A	INTRINSIC
low complexity region	1807	1822	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2071	2089	N/A	INTRINSIC
HLH	2198	2249	8.27e-7	SMART
low complexity region	2578	2599	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000110773
AA Change: I351V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000106400
Gene: ENSMUSG00000033943
AA Change: I351V

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	5e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	890	899	N/A	INTRINSIC
low complexity region	938	952	N/A	INTRINSIC
low complexity region	1033	1059	N/A	INTRINSIC
low complexity region	1169	1190	N/A	INTRINSIC
low complexity region	1222	1236	N/A	INTRINSIC
low complexity region	1485	1502	N/A	INTRINSIC
low complexity region	1555	1570	N/A	INTRINSIC
low complexity region	1602	1637	N/A	INTRINSIC
low complexity region	1717	1739	N/A	INTRINSIC
low complexity region	1754	1771	N/A	INTRINSIC
low complexity region	1898	1913	N/A	INTRINSIC
low complexity region	2104	2118	N/A	INTRINSIC
low complexity region	2162	2180	N/A	INTRINSIC
HLH	2289	2340	8.27e-7	SMART
low complexity region	2669	2690	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000110774
AA Change: I351V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000106401
Gene: ENSMUSG00000033943
AA Change: I351V

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	7e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
Pfam:DUF4801	1037	1085	1e-19	PFAM
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1248	1269	N/A	INTRINSIC
low complexity region	1301	1315	N/A	INTRINSIC
low complexity region	1564	1581	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC
low complexity region	1681	1716	N/A	INTRINSIC
low complexity region	1835	1857	N/A	INTRINSIC
low complexity region	1872	1889	N/A	INTRINSIC
low complexity region	2016	2031	N/A	INTRINSIC
low complexity region	2222	2236	N/A	INTRINSIC
low complexity region	2280	2298	N/A	INTRINSIC
HLH	2407	2458	8.27e-7	SMART
low complexity region	2787	2808	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129405

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141776

Predicted Effect

probably benign
Transcript: ENSMUST00000156510
AA Change: I351V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000119044
Gene: ENSMUSG00000033943
AA Change: I351V

Domain	Start	End	E-Value	Type
Blast:TBOX	6	73	4e-27	BLAST
TBOX	74	265	2.34e-100	SMART
low complexity region	969	978	N/A	INTRINSIC
low complexity region	1017	1031	N/A	INTRINSIC
low complexity region	1112	1138	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1300	1314	N/A	INTRINSIC
low complexity region	1626	1648	N/A	INTRINSIC
low complexity region	1663	1680	N/A	INTRINSIC
low complexity region	1807	1822	N/A	INTRINSIC
low complexity region	2013	2027	N/A	INTRINSIC
low complexity region	2071	2089	N/A	INTRINSIC
HLH	2198	2249	8.27e-7	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Embryos homozygous for a gene trap allele die shortly after implantation due to defective development of the inner cell mass (ICM) and the epiblast. ICM derivatives fail to develop past E4.5 and show increased apoptosis but no change in cell proliferation. [provided by MGI curators]

Allele List at MGI

All alleles(135) : Gene trapped(135)

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503B20Rik	A	G	3: 146,646,424 (GRCm38)	L228S	probably damaging	Het
Abca13	A	T	11: 9,328,136 (GRCm38)	K3105N	probably damaging	Het
Abca3	T	A	17: 24,376,726 (GRCm38)	L351Q	possibly damaging	Het
Acadm	A	G	3: 153,933,043 (GRCm38)	I231T	possibly damaging	Het
Adamts4	A	T	1: 171,256,229 (GRCm38)	Q456L	probably damaging	Het
Adgra3	C	T	5: 50,001,880 (GRCm38)	V343I	probably benign	Het
Ajuba	C	A	14: 54,577,188 (GRCm38)	G26V	probably damaging	Het
Aldh1a1	A	G	19: 20,620,091 (GRCm38)	I145M	possibly damaging	Het
Ankrd42	G	A	7: 92,625,773 (GRCm38)	Q110*	probably null	Het
Ano3	T	A	2: 110,682,759 (GRCm38)	E630D	probably benign	Het
Ap3d1	T	C	10: 80,713,998 (GRCm38)	D771G	probably damaging	Het
Arhgap30	A	G	1: 171,389,328 (GRCm38)	D21G	probably damaging	Het
Atg13	T	C	2: 91,679,425 (GRCm38)	N374S	probably benign	Het
Ccser1	A	T	6: 61,570,815 (GRCm38)	M49L	probably damaging	Het
Cdh5	A	G	8: 104,125,733 (GRCm38)	E160G	probably damaging	Het
Cpd	T	C	11: 76,797,801 (GRCm38)	K882E	probably benign	Het
Crtac1	G	A	19: 42,283,567 (GRCm38)	L646F	unknown	Het
Cyp27b1	T	G	10: 127,048,294 (GRCm38)	V5G	probably damaging	Het
Dcaf11	G	T	14: 55,569,371 (GRCm38)	*550Y	probably null	Het
Dhx58	A	G	11: 100,698,154 (GRCm38)		probably null	Het
Dlg2	T	A	7: 92,438,041 (GRCm38)	I881N	probably damaging	Het
Ece1	C	T	4: 137,946,362 (GRCm38)	T407M	possibly damaging	Het
Ecsit	C	T	9: 22,076,540 (GRCm38)	V68I	possibly damaging	Het
Egln1	T	C	8: 124,948,414 (GRCm38)	D214G	probably benign	Het
Espn	T	C	4: 152,135,545 (GRCm38)	T47A	possibly damaging	Het
F5	A	G	1: 164,195,720 (GRCm38)	I1616V	possibly damaging	Het
Fam43b	G	C	4: 138,395,098 (GRCm38)	R304G	probably benign	Het
Flnc	G	A	6: 29,438,666 (GRCm38)	W186*	probably null	Het
Fryl	T	C	5: 73,041,364 (GRCm38)	D2640G	possibly damaging	Het
Gabrg2	T	C	11: 41,976,636 (GRCm38)	K52R	possibly damaging	Het
Ganab	T	C	19: 8,909,468 (GRCm38)	V306A	probably damaging	Het
Glod4	A	T	11: 76,237,809 (GRCm38)	I70N	possibly damaging	Het
Gm8251	T	A	1: 44,056,460 (GRCm38)	H1826L	possibly damaging	Het
Gsn	T	A	2: 35,283,918 (GRCm38)	V2E	probably benign	Het
Hist1h1c	A	G	13: 23,738,924 (GRCm38)	K26E	probably benign	Het
Hrg	C	T	16: 22,961,309 (GRCm38)		probably benign	Het
Kctd19	T	C	8: 105,387,266 (GRCm38)	T592A	probably benign	Het
Kdm3b	A	G	18: 34,808,419 (GRCm38)	D521G	probably damaging	Het
Lrba	A	G	3: 86,776,103 (GRCm38)	H2744R	possibly damaging	Het
Lsr	C	T	7: 30,958,345 (GRCm38)	A397T	probably damaging	Het
Methig1	A	T	15: 100,353,360 (GRCm38)	M51L	possibly damaging	Het
Mlf1	G	A	3: 67,399,751 (GRCm38)	V250I	possibly damaging	Het
Mreg	A	T	1: 72,192,064 (GRCm38)	N78K	probably damaging	Het
Mtor	A	G	4: 148,489,555 (GRCm38)	D1294G	probably benign	Het
Nfkb1	T	C	3: 135,601,521 (GRCm38)	I548V	probably damaging	Het
Nlrp3	T	C	11: 59,549,136 (GRCm38)	F513S	possibly damaging	Het
Nphp4	A	G	4: 152,557,043 (GRCm38)	K1094E	possibly damaging	Het
Nr3c2	C	A	8: 76,909,907 (GRCm38)	H546N	probably damaging	Het
Nr4a2	T	A	2: 57,112,199 (GRCm38)	M18L	probably benign	Het
Olfr1214	G	A	2: 88,987,470 (GRCm38)	T244I	probably benign	Het
Olfr1392	A	C	11: 49,293,632 (GRCm38)	T104P	probably benign	Het
Pclo	A	G	5: 14,540,332 (GRCm38)	N882S	unknown	Het
Pde6a	T	C	18: 61,262,434 (GRCm38)	Y583H	probably damaging	Het
Pip5k1b	T	A	19: 24,378,947 (GRCm38)	Y209F	probably damaging	Het
Plch2	A	T	4: 154,984,309 (GRCm38)	S1182T	probably damaging	Het
Ptger2	G	T	14: 44,989,650 (GRCm38)	R229L	probably damaging	Het
Ptpru	C	A	4: 131,808,499 (GRCm38)	G389V	probably damaging	Het
Ric8a	A	G	7: 140,861,938 (GRCm38)	N249S	probably benign	Het
Slc26a5	A	G	5: 21,831,510 (GRCm38)	I266T	possibly damaging	Het
Smyd1	T	C	6: 71,238,676 (GRCm38)	N100D	probably damaging	Het
Stag3	T	C	5: 138,298,284 (GRCm38)	F468S	probably damaging	Het
Stk31	C	T	6: 49,417,250 (GRCm38)	T182I	probably damaging	Het
Svep1	G	T	4: 58,082,677 (GRCm38)	N1982K	possibly damaging	Het
Thegl	T	C	5: 77,059,367 (GRCm38)	I324T	probably damaging	Het
Tinagl1	A	G	4: 130,166,993 (GRCm38)	Y344H	probably damaging	Het
Tmem216	T	A	19: 10,551,873 (GRCm38)	T104S	probably damaging	Het
Tmem231	T	C	8: 111,918,931 (GRCm38)	D112G	probably damaging	Het
Ube4b	A	G	4: 149,344,572 (GRCm38)	I870T	probably damaging	Het
Vwa8	T	A	14: 79,064,996 (GRCm38)	L1035Q	possibly damaging	Het
Wdr35	A	G	12: 8,978,628 (GRCm38)	Q82R	probably benign	Het
Wnk1	A	T	6: 119,963,640 (GRCm38)		probably null	Het
Zc3h7a	T	C	16: 11,158,594 (GRCm38)		probably benign	Het
Zfp532	T	C	18: 65,624,712 (GRCm38)	V572A	probably damaging	Het

Other mutations in Mga

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Mga	APN	2	119,919,814 (GRCm38)	missense	possibly damaging	0.65
IGL00719:Mga	APN	2	119,947,453 (GRCm38)	nonsense	probably null
IGL01619:Mga	APN	2	119,931,828 (GRCm38)	missense	possibly damaging	0.46
IGL01721:Mga	APN	2	119,935,239 (GRCm38)	missense	probably damaging	1.00
IGL01759:Mga	APN	2	119,951,195 (GRCm38)	missense	possibly damaging	0.92
IGL01785:Mga	APN	2	119,902,912 (GRCm38)	missense	probably damaging	1.00
IGL01786:Mga	APN	2	119,902,912 (GRCm38)	missense	probably damaging	1.00
IGL01950:Mga	APN	2	119,941,654 (GRCm38)	missense	possibly damaging	0.60
IGL01960:Mga	APN	2	119,938,657 (GRCm38)	missense	probably damaging	1.00
IGL02086:Mga	APN	2	119,924,036 (GRCm38)	missense	probably damaging	0.99
IGL02364:Mga	APN	2	119,964,054 (GRCm38)	missense	possibly damaging	0.66
IGL02602:Mga	APN	2	119,931,884 (GRCm38)	missense	possibly damaging	0.66
IGL02751:Mga	APN	2	119,947,770 (GRCm38)	missense	possibly damaging	0.82
IGL02794:Mga	APN	2	119,946,289 (GRCm38)	missense	possibly damaging	0.84
IGL03247:Mga	APN	2	119,935,513 (GRCm38)	missense	possibly damaging	0.81
IGL03303:Mga	APN	2	119,903,452 (GRCm38)	missense	probably damaging	1.00
PIT4515001:Mga	UTSW	2	119,916,504 (GRCm38)	missense	probably damaging	1.00
R0060:Mga	UTSW	2	119,960,961 (GRCm38)	critical splice donor site	probably null
R0060:Mga	UTSW	2	119,960,961 (GRCm38)	critical splice donor site	probably null
R0417:Mga	UTSW	2	119,902,790 (GRCm38)	missense	probably damaging	0.99
R0449:Mga	UTSW	2	119,941,381 (GRCm38)	missense	probably damaging	1.00
R0457:Mga	UTSW	2	119,916,488 (GRCm38)	missense	probably damaging	0.98
R0538:Mga	UTSW	2	119,919,706 (GRCm38)	critical splice donor site	probably null
R0568:Mga	UTSW	2	119,935,422 (GRCm38)	missense	probably damaging	1.00
R0614:Mga	UTSW	2	119,964,466 (GRCm38)	missense	probably damaging	1.00
R0671:Mga	UTSW	2	119,919,910 (GRCm38)	splice site	probably null
R0811:Mga	UTSW	2	119,947,961 (GRCm38)	missense	probably damaging	0.99
R0812:Mga	UTSW	2	119,947,961 (GRCm38)	missense	probably damaging	0.99
R0948:Mga	UTSW	2	119,941,659 (GRCm38)	missense	possibly damaging	0.77
R1177:Mga	UTSW	2	119,926,446 (GRCm38)	missense	probably damaging	1.00
R1445:Mga	UTSW	2	119,902,698 (GRCm38)	missense	probably damaging	1.00
R1476:Mga	UTSW	2	119,941,675 (GRCm38)	missense	probably damaging	0.96
R1527:Mga	UTSW	2	119,916,597 (GRCm38)	missense	probably damaging	1.00
R1583:Mga	UTSW	2	119,963,960 (GRCm38)	missense	possibly damaging	0.66
R1592:Mga	UTSW	2	119,964,666 (GRCm38)	missense	possibly damaging	0.93
R1627:Mga	UTSW	2	119,964,562 (GRCm38)	missense	probably damaging	1.00
R1658:Mga	UTSW	2	119,941,689 (GRCm38)	missense	possibly damaging	0.63
R1677:Mga	UTSW	2	119,960,852 (GRCm38)	missense	possibly damaging	0.92
R1887:Mga	UTSW	2	119,923,617 (GRCm38)	missense	probably damaging	1.00
R1908:Mga	UTSW	2	119,926,594 (GRCm38)	missense	possibly damaging	0.66
R1909:Mga	UTSW	2	119,926,594 (GRCm38)	missense	possibly damaging	0.66
R2061:Mga	UTSW	2	119,964,980 (GRCm38)	unclassified	probably benign
R2145:Mga	UTSW	2	119,964,157 (GRCm38)	missense	possibly damaging	0.85
R2159:Mga	UTSW	2	119,919,643 (GRCm38)	missense	probably damaging	0.96
R2179:Mga	UTSW	2	119,960,442 (GRCm38)	missense	probably damaging	0.99
R2423:Mga	UTSW	2	119,964,793 (GRCm38)	missense	probably damaging	1.00
R3620:Mga	UTSW	2	119,916,668 (GRCm38)	missense	probably damaging	1.00
R3622:Mga	UTSW	2	119,941,764 (GRCm38)	missense	probably damaging	1.00
R3624:Mga	UTSW	2	119,941,764 (GRCm38)	missense	probably damaging	1.00
R3802:Mga	UTSW	2	119,947,339 (GRCm38)	missense	probably damaging	0.96
R4011:Mga	UTSW	2	119,931,780 (GRCm38)	missense	probably damaging	1.00
R4065:Mga	UTSW	2	119,947,002 (GRCm38)	missense	probably damaging	1.00
R4520:Mga	UTSW	2	119,948,098 (GRCm38)	missense	possibly damaging	0.85
R4649:Mga	UTSW	2	119,941,493 (GRCm38)	missense	possibly damaging	0.81
R4660:Mga	UTSW	2	119,938,623 (GRCm38)	intron	probably benign
R4757:Mga	UTSW	2	119,903,639 (GRCm38)	missense	possibly damaging	0.82
R4771:Mga	UTSW	2	119,964,294 (GRCm38)	missense	probably damaging	1.00
R4784:Mga	UTSW	2	119,903,057 (GRCm38)	missense	probably damaging	1.00
R4866:Mga	UTSW	2	119,964,054 (GRCm38)	missense	possibly damaging	0.66
R4900:Mga	UTSW	2	119,964,054 (GRCm38)	missense	possibly damaging	0.66
R4952:Mga	UTSW	2	119,903,301 (GRCm38)	missense	probably damaging	1.00
R4995:Mga	UTSW	2	119,932,582 (GRCm38)	nonsense	probably null
R5020:Mga	UTSW	2	119,951,173 (GRCm38)	nonsense	probably null
R5082:Mga	UTSW	2	119,903,344 (GRCm38)	missense	probably damaging	0.98
R5208:Mga	UTSW	2	119,947,981 (GRCm38)	missense	possibly damaging	0.83
R5454:Mga	UTSW	2	119,903,329 (GRCm38)	missense	probably damaging	0.99
R5466:Mga	UTSW	2	119,902,697 (GRCm38)	missense	probably damaging	1.00
R5484:Mga	UTSW	2	119,916,626 (GRCm38)	missense	possibly damaging	0.58
R5669:Mga	UTSW	2	119,903,426 (GRCm38)	missense	probably damaging	1.00
R5819:Mga	UTSW	2	119,941,263 (GRCm38)	missense	possibly damaging	0.61
R5916:Mga	UTSW	2	119,964,312 (GRCm38)	missense	probably benign	0.27
R5942:Mga	UTSW	2	119,946,959 (GRCm38)	missense	probably benign	0.41
R6305:Mga	UTSW	2	119,947,698 (GRCm38)	missense	probably benign	0.00
R6434:Mga	UTSW	2	119,923,938 (GRCm38)	missense	probably damaging	0.99
R6467:Mga	UTSW	2	119,946,295 (GRCm38)	missense	probably damaging	1.00
R6488:Mga	UTSW	2	119,960,907 (GRCm38)	missense	probably damaging	1.00
R6630:Mga	UTSW	2	119,923,659 (GRCm38)	missense	probably damaging	0.99
R6790:Mga	UTSW	2	119,923,754 (GRCm38)	missense	probably damaging	0.99
R7029:Mga	UTSW	2	119,923,550 (GRCm38)	missense	probably damaging	1.00
R7039:Mga	UTSW	2	119,932,678 (GRCm38)	missense	probably benign	0.28
R7088:Mga	UTSW	2	119,961,936 (GRCm38)	missense	probably damaging	1.00
R7195:Mga	UTSW	2	119,917,328 (GRCm38)	missense	probably damaging	1.00
R7273:Mga	UTSW	2	119,935,214 (GRCm38)	missense	probably damaging	1.00
R7286:Mga	UTSW	2	119,964,788 (GRCm38)	missense	possibly damaging	0.93
R7346:Mga	UTSW	2	119,935,527 (GRCm38)	missense	possibly damaging	0.56
R7383:Mga	UTSW	2	119,960,340 (GRCm38)	missense	probably damaging	0.99
R7469:Mga	UTSW	2	119,903,046 (GRCm38)	missense	probably damaging	1.00
R7484:Mga	UTSW	2	119,946,229 (GRCm38)	missense	probably damaging	0.99
R7537:Mga	UTSW	2	119,935,551 (GRCm38)	missense	probably damaging	0.97
R7781:Mga	UTSW	2	119,917,357 (GRCm38)	missense	probably damaging	1.00
R7921:Mga	UTSW	2	119,919,678 (GRCm38)	missense	probably damaging	1.00
R8165:Mga	UTSW	2	119,947,238 (GRCm38)	missense	probably benign	0.12
R8226:Mga	UTSW	2	119,960,385 (GRCm38)	missense	probably benign	0.33
R8305:Mga	UTSW	2	119,946,319 (GRCm38)	missense	possibly damaging	0.77
R8309:Mga	UTSW	2	119,960,930 (GRCm38)	missense	probably damaging	1.00
R8363:Mga	UTSW	2	119,963,926 (GRCm38)	missense	probably benign	0.43
R8388:Mga	UTSW	2	119,964,081 (GRCm38)	missense	probably benign	0.00
R8524:Mga	UTSW	2	119,941,516 (GRCm38)	missense	probably damaging	0.97
R8693:Mga	UTSW	2	119,963,926 (GRCm38)	missense	possibly damaging	0.65
R8837:Mga	UTSW	2	119,938,791 (GRCm38)	splice site	probably benign
R8916:Mga	UTSW	2	119,958,338 (GRCm38)	missense	possibly damaging	0.92
R8936:Mga	UTSW	2	119,964,228 (GRCm38)	missense	probably damaging	1.00
R9028:Mga	UTSW	2	119,947,589 (GRCm38)	missense	probably benign
R9145:Mga	UTSW	2	119,964,012 (GRCm38)	missense	probably benign
R9155:Mga	UTSW	2	119,926,532 (GRCm38)	missense	probably damaging	1.00
R9308:Mga	UTSW	2	119,923,888 (GRCm38)	missense	possibly damaging	0.91
R9342:Mga	UTSW	2	119,948,175 (GRCm38)	missense	probably benign
R9347:Mga	UTSW	2	119,903,037 (GRCm38)	missense	probably damaging	1.00
R9390:Mga	UTSW	2	119,963,851 (GRCm38)	missense	probably damaging	0.99
R9408:Mga	UTSW	2	119,935,518 (GRCm38)	missense	possibly damaging	0.92
R9488:Mga	UTSW	2	119,964,823 (GRCm38)	missense	possibly damaging	0.90
R9495:Mga	UTSW	2	119,951,195 (GRCm38)	missense	possibly damaging	0.92
R9521:Mga	UTSW	2	119,964,498 (GRCm38)	missense	probably damaging	0.99
R9780:Mga	UTSW	2	119,916,772 (GRCm38)	missense	probably benign	0.26

Predicted Primers

PCR Primer
(F):5'- ATCGTGTTCGTCTTACAGAAGGTG -3'
(R):5'- ATTCCAAAATCTGGCCCATCTC -3'

Sequencing Primer
(F):5'- TCGTCTTACAGAAGGTGAAGGGTC -3'
(R):5'- TGGCCCATCTCAATCTTTTTATTTC -3'

Posted On

2014-10-16