Mutagenetix > Incidental Mutation

Incidental Mutation 'R2284:Vmn2r45'

243241

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r45

Ensembl Gene

ENSMUSG00000090662

Gene Name

vomeronasal 2, receptor 45

Synonyms

MMRRC Submission

040283-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R2284 (G1)

Quality Score

205

Status

Not validated

Chromosome

Chromosomal Location

8474468-8491958 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 8488765 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 88 (N88K)

Ref Sequence

ENSEMBL: ENSMUSP00000129466 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164845]

AlphaFold

L7N2B5

Predicted Effect

probably benign
Transcript: ENSMUST00000164845
AA Change: N88K

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000129466
Gene: ENSMUSG00000090662
AA Change: N88K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	469	4.5e-26	PFAM
Pfam:NCD3G	512	565	6.4e-21	PFAM
Pfam:7tm_3	598	833	2.1e-54	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adipoq	C	T	16: 22,976,237 (GRCm39)	Q213*	probably null	Het
Adsl	C	T	15: 80,848,096 (GRCm39)	P278L	probably damaging	Het
Bcas2	T	C	3: 103,085,678 (GRCm39)	S187P	probably damaging	Het
Ccdc88a	T	C	11: 29,444,099 (GRCm39)		probably null	Het
Cryzl1	C	T	16: 91,491,193 (GRCm39)		probably benign	Het
Csl	T	A	10: 99,594,321 (GRCm39)	D248V	possibly damaging	Het
Dip2a	A	C	10: 76,149,027 (GRCm39)	V247G	probably benign	Het
Fat2	T	C	11: 55,173,186 (GRCm39)	D2509G	probably damaging	Het
Gldn	G	A	9: 54,193,849 (GRCm39)	W14*	probably null	Het
Gm5698	C	T	1: 31,016,964 (GRCm39)	R29Q	possibly damaging	Het
Gm5830	A	T	1: 78,945,415 (GRCm39)		noncoding transcript	Het
Gtf2ird2	A	G	5: 134,246,025 (GRCm39)	D761G	probably benign	Het
Hdac10	T	C	15: 89,011,607 (GRCm39)	Q159R	probably benign	Het
Hhatl	T	C	9: 121,618,648 (GRCm39)	Y118C	probably damaging	Het
Iho1	C	T	9: 108,298,672 (GRCm39)	E49K	probably damaging	Het
Klrd1	A	G	6: 129,575,344 (GRCm39)	H127R	probably benign	Het
Krtap31-1	C	T	11: 99,799,081 (GRCm39)	Q95*	probably null	Het
Map2	C	T	1: 66,453,227 (GRCm39)	P548S	probably damaging	Het
Mki67	A	G	7: 135,301,674 (GRCm39)	V1120A	probably damaging	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Mtmr12	T	A	15: 12,245,097 (GRCm39)	M204K	probably damaging	Het
Muc6	T	C	7: 141,217,837 (GRCm39)	T2279A	possibly damaging	Het
Naip6	C	T	13: 100,437,108 (GRCm39)	A472T	probably benign	Het
Nlrp1b	A	G	11: 71,047,110 (GRCm39)	S1084P	probably benign	Het
Nr2f1	C	A	13: 78,343,581 (GRCm39)	V81F	probably damaging	Het
Nrxn3	T	A	12: 89,477,135 (GRCm39)	N803K	probably damaging	Het
Or5l14	A	G	2: 87,793,137 (GRCm39)	L33P	probably damaging	Het
Ptpre	A	G	7: 135,271,510 (GRCm39)	H375R	probably benign	Het
Tsc1	G	A	2: 28,555,109 (GRCm39)	V200I	possibly damaging	Het
Vmn1r42	A	G	6: 89,821,681 (GRCm39)	I296T	probably benign	Het
Vmn1r67	A	T	7: 10,181,600 (GRCm39)	H288L	probably damaging	Het
Wt1	A	G	2: 105,002,666 (GRCm39)	T511A	probably benign	Het

Other mutations in Vmn2r45

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01287:Vmn2r45	APN	7	8,488,622 (GRCm39)	missense	probably benign	0.00
IGL01322:Vmn2r45	APN	7	8,484,332 (GRCm39)	missense	possibly damaging	0.49
IGL01402:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01404:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01529:Vmn2r45	APN	7	8,486,493 (GRCm39)	missense	probably benign
IGL01596:Vmn2r45	APN	7	8,486,272 (GRCm39)	missense	probably damaging	0.98
IGL01646:Vmn2r45	APN	7	8,486,337 (GRCm39)	missense	probably benign	0.18
IGL01819:Vmn2r45	APN	7	8,488,556 (GRCm39)	missense	probably benign	0.00
IGL02096:Vmn2r45	APN	7	8,484,443 (GRCm39)	missense	probably damaging	0.99
IGL02130:Vmn2r45	APN	7	8,486,556 (GRCm39)	missense	possibly damaging	0.46
IGL02409:Vmn2r45	APN	7	8,488,727 (GRCm39)	missense	probably benign	0.03
IGL02517:Vmn2r45	APN	7	8,486,185 (GRCm39)	nonsense	probably null
IGL02633:Vmn2r45	APN	7	8,488,728 (GRCm39)	missense	probably benign	0.01
IGL02940:Vmn2r45	APN	7	8,475,369 (GRCm39)	missense	probably damaging	0.99
IGL03281:Vmn2r45	APN	7	8,486,603 (GRCm39)	missense	probably damaging	1.00
IGL03358:Vmn2r45	APN	7	8,474,715 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
BB014:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R0382:Vmn2r45	UTSW	7	8,486,098 (GRCm39)	missense	probably benign	0.06
R0532:Vmn2r45	UTSW	7	8,474,820 (GRCm39)	missense	probably damaging	0.99
R0932:Vmn2r45	UTSW	7	8,478,380 (GRCm39)	missense	probably damaging	1.00
R1125:Vmn2r45	UTSW	7	8,488,542 (GRCm39)	missense	probably benign	0.00
R1580:Vmn2r45	UTSW	7	8,474,746 (GRCm39)	missense	possibly damaging	0.95
R1817:Vmn2r45	UTSW	7	8,475,372 (GRCm39)	missense	probably damaging	1.00
R1996:Vmn2r45	UTSW	7	8,475,024 (GRCm39)	missense	probably damaging	1.00
R2050:Vmn2r45	UTSW	7	8,475,021 (GRCm39)	missense	probably damaging	1.00
R4019:Vmn2r45	UTSW	7	8,474,580 (GRCm39)	nonsense	probably null
R4227:Vmn2r45	UTSW	7	8,486,277 (GRCm39)	missense	probably damaging	0.98
R4381:Vmn2r45	UTSW	7	8,474,912 (GRCm39)	nonsense	probably null
R4618:Vmn2r45	UTSW	7	8,486,436 (GRCm39)	missense	probably benign	0.00
R4624:Vmn2r45	UTSW	7	8,484,341 (GRCm39)	missense	probably damaging	1.00
R4704:Vmn2r45	UTSW	7	8,486,535 (GRCm39)	nonsense	probably null
R4735:Vmn2r45	UTSW	7	8,486,472 (GRCm39)	missense	probably damaging	1.00
R4868:Vmn2r45	UTSW	7	8,484,480 (GRCm39)	missense	probably benign	0.06
R4983:Vmn2r45	UTSW	7	8,486,116 (GRCm39)	missense	probably damaging	0.99
R5246:Vmn2r45	UTSW	7	8,486,251 (GRCm39)	missense	probably benign	0.00
R5430:Vmn2r45	UTSW	7	8,486,333 (GRCm39)	nonsense	probably null
R5504:Vmn2r45	UTSW	7	8,486,176 (GRCm39)	missense	probably benign	0.03
R5511:Vmn2r45	UTSW	7	8,474,832 (GRCm39)	missense	probably benign	0.19
R5745:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R5814:Vmn2r45	UTSW	7	8,474,475 (GRCm39)	missense	probably benign	0.00
R6223:Vmn2r45	UTSW	7	8,486,301 (GRCm39)	missense	probably benign
R6267:Vmn2r45	UTSW	7	8,475,207 (GRCm39)	missense	probably benign	0.26
R6623:Vmn2r45	UTSW	7	8,474,500 (GRCm39)	missense	probably benign	0.09
R6999:Vmn2r45	UTSW	7	8,486,219 (GRCm39)	missense	probably benign
R7242:Vmn2r45	UTSW	7	8,488,612 (GRCm39)	nonsense	probably null
R7491:Vmn2r45	UTSW	7	8,484,342 (GRCm39)	missense	probably benign	0.27
R7620:Vmn2r45	UTSW	7	8,486,222 (GRCm39)	nonsense	probably null
R7719:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7720:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7853:Vmn2r45	UTSW	7	8,485,987 (GRCm39)	missense	possibly damaging	0.90
R7873:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R7882:Vmn2r45	UTSW	7	8,486,409 (GRCm39)	missense	possibly damaging	0.82
R7927:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R8684:Vmn2r45	UTSW	7	8,486,511 (GRCm39)	missense	probably damaging	0.99
R8780:Vmn2r45	UTSW	7	8,484,371 (GRCm39)	missense	possibly damaging	0.94
R8811:Vmn2r45	UTSW	7	8,474,881 (GRCm39)	missense	probably damaging	1.00
R8893:Vmn2r45	UTSW	7	8,488,619 (GRCm39)	missense	probably damaging	1.00
R9043:Vmn2r45	UTSW	7	8,474,904 (GRCm39)	missense	possibly damaging	0.92
R9208:Vmn2r45	UTSW	7	8,486,298 (GRCm39)	missense	probably damaging	1.00
R9327:Vmn2r45	UTSW	7	8,474,532 (GRCm39)	missense	probably damaging	1.00
R9586:Vmn2r45	UTSW	7	8,486,050 (GRCm39)	missense	probably benign	0.01
R9599:Vmn2r45	UTSW	7	8,478,457 (GRCm39)	critical splice acceptor site	probably null
U24488:Vmn2r45	UTSW	7	8,475,361 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r45	UTSW	7	8,474,484 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- TGGAGATAAGAAGCTGTTCACG -3'
(R):5'- CCCGTCTTTAGATGTCAGATTCAG -3'

Sequencing Primer
(F):5'- GATAAGAAGCTGTTCACGTGCTTCC -3'
(R):5'- CAGAAACTTCCAGTATAGCATAGTGC -3'

Posted On

2014-10-16