Mutagenetix > Incidental Mutation

Incidental Mutation 'R2259:Unc13b'

243615

Institutional Source

Beutler Lab

Gene Symbol

Unc13b

Ensembl Gene

ENSMUSG00000028456

Gene Name

unc-13 homolog B (C. elegans)

Synonyms

Unc13h2, Munc13-2

MMRRC Submission

040259-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.317) question?

Stock #

R2259 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

43058953-43264871 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 43182780 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Valine at position 3163 (E3163V)

Ref Sequence

ENSEMBL: ENSMUSP00000147100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079978] [ENSMUST00000107952] [ENSMUST00000107953] [ENSMUST00000163653] [ENSMUST00000207569]

AlphaFold

Q9Z1N9

Predicted Effect

probably benign
Transcript: ENSMUST00000079978
AA Change: E363V

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000078894
Gene: ENSMUSG00000028456
AA Change: E363V

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	179	193	N/A	INTRINSIC
low complexity region	292	303	N/A	INTRINSIC
low complexity region	322	333	N/A	INTRINSIC
C1	478	527	4.21e-18	SMART
C2	601	708	2.07e-22	SMART
DUF1041	917	1021	2.02e-53	SMART
Pfam:Membr_traf_MHD	1262	1404	4.8e-60	PFAM
C2	1438	1544	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107952
AA Change: E375V

PolyPhen 2 Score 0.105 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000103586
Gene: ENSMUSG00000028456
AA Change: E375V

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	191	205	N/A	INTRINSIC
low complexity region	304	315	N/A	INTRINSIC
low complexity region	334	345	N/A	INTRINSIC
C1	490	539	4.21e-18	SMART
C2	613	720	2.07e-22	SMART
DUF1041	929	1033	2.02e-53	SMART
Pfam:Membr_traf_MHD	1274	1416	4.8e-60	PFAM
C2	1450	1556	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107953
AA Change: E363V

PolyPhen 2 Score 0.125 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000103587
Gene: ENSMUSG00000028456
AA Change: E363V

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	179	193	N/A	INTRINSIC
low complexity region	292	303	N/A	INTRINSIC
low complexity region	322	333	N/A	INTRINSIC
C1	478	527	4.21e-18	SMART
C2	601	708	2.07e-22	SMART
DUF1041	917	1021	2.02e-53	SMART
Pfam:Membr_traf_MHD	1263	1403	2.3e-56	PFAM
C2	1457	1563	7.56e-16	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132310

Predicted Effect

probably benign
Transcript: ENSMUST00000163653
AA Change: E375V

PolyPhen 2 Score 0.105 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000128608
Gene: ENSMUSG00000028456
AA Change: E375V

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	191	205	N/A	INTRINSIC
low complexity region	304	315	N/A	INTRINSIC
low complexity region	334	345	N/A	INTRINSIC
C1	490	539	4.21e-18	SMART
C2	613	720	2.07e-22	SMART
DUF1041	929	1032	4.64e-53	SMART
Pfam:Membr_traf_MHD	1273	1415	4.8e-60	PFAM
C2	1449	1555	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000168032
AA Change: E29V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000132622
Gene: ENSMUSG00000028456
AA Change: E29V

Domain	Start	End	E-Value	Type
C1	147	196	4.21e-18	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000207569
AA Change: E3163V

PolyPhen 2 Score 0.871 (Sensitivity: 0.83; Specificity: 0.93)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice are grossly phenotypically normal. Mice older than 12 months will exhibit sporadic seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrd1	T	G	5: 129,112,311 (GRCm38)	S91A	possibly damaging	Het
Ankrd13b	T	G	11: 77,476,342 (GRCm38)	N247T	probably damaging	Het
Atp10b	G	A	11: 43,189,613 (GRCm38)	V239M	probably damaging	Het
Atp10b	A	G	11: 43,172,745 (GRCm38)	D169G	probably damaging	Het
Cflar	C	T	1: 58,729,121 (GRCm38)	T121I	probably benign	Het
Clca3b	T	C	3: 144,846,381 (GRCm38)	N180D	possibly damaging	Het
Cnbd2	T	A	2: 156,335,272 (GRCm38)	I62N	probably damaging	Het
Col11a2	A	G	17: 34,039,677 (GRCm38)	H8R	probably benign	Het
Cyp2a4	C	T	7: 26,309,035 (GRCm38)	L201F	probably damaging	Het
D630003M21Rik	A	T	2: 158,204,711 (GRCm38)	L782Q	probably damaging	Het
Dctn1	C	A	6: 83,197,586 (GRCm38)	H1065N	possibly damaging	Het
Dgcr2	A	T	16: 17,844,977 (GRCm38)		probably null	Het
Dlg4	T	C	11: 70,031,370 (GRCm38)	I143T	probably damaging	Het
E2f7	T	G	10: 110,746,343 (GRCm38)	N4K	probably damaging	Het
Eef2kmt	C	T	16: 5,245,308 (GRCm38)	V323I	probably benign	Het
Eif2ak4	A	C	2: 118,455,783 (GRCm38)	I1017L	probably damaging	Het
Eln	C	A	5: 134,729,654 (GRCm38)	A126S	unknown	Het
Exoc7	T	C	11: 116,306,411 (GRCm38)	S35G	probably damaging	Het
Fam187a	A	G	11: 102,885,298 (GRCm38)		probably benign	Het
Fkbp6	C	T	5: 135,337,614 (GRCm38)		probably null	Het
Flnc	G	A	6: 29,438,666 (GRCm38)	W186*	probably null	Het
Fmn2	T	A	1: 174,502,932 (GRCm38)	L296H	unknown	Het
Galnt14	C	A	17: 73,494,266 (GRCm38)	M520I	probably benign	Het
Gba2	A	G	4: 43,570,107 (GRCm38)	C396R	probably benign	Het
Gigyf1	C	A	5: 137,520,332 (GRCm38)	A215E	possibly damaging	Het
Glb1	C	T	9: 114,443,032 (GRCm38)	Q246*	probably null	Het
Gm11565	T	G	11: 99,915,018 (GRCm38)	C79G	possibly damaging	Het
Gpr160	A	G	3: 30,896,295 (GRCm38)	Y172C	probably damaging	Het
Ift52	A	G	2: 163,028,093 (GRCm38)	N159S	probably benign	Het
Insrr	A	G	3: 87,800,452 (GRCm38)	D67G	probably damaging	Het
Insyn2a	T	A	7: 134,917,667 (GRCm38)	E378V	probably damaging	Het
Irf2	A	G	8: 46,837,833 (GRCm38)	Y230C	probably benign	Het
Jmjd6	T	C	11: 116,841,314 (GRCm38)	H187R	probably damaging	Het
Kdm2b	C	T	5: 122,882,416 (GRCm38)	G90S	probably damaging	Het
Kif7	C	T	7: 79,711,589 (GRCm38)	G118D	probably damaging	Het
Klhl38	G	C	15: 58,314,978 (GRCm38)	T532S	possibly damaging	Het
Kmt2a	G	T	9: 44,881,142 (GRCm38)		probably benign	Het
Lama2	A	G	10: 27,031,127 (GRCm38)	L2346S	probably benign	Het
Marveld2	A	G	13: 100,612,470 (GRCm38)	S34P	probably benign	Het
Mllt6	T	C	11: 97,664,976 (GRCm38)	V44A	probably damaging	Het
Muc5ac	A	G	7: 141,791,008 (GRCm38)	N72S	probably benign	Het
Myo7a	T	G	7: 98,069,499 (GRCm38)	D1388A	probably damaging	Het
Ncapd3	A	G	9: 27,056,072 (GRCm38)	D568G	probably benign	Het
Ncoa1	A	C	12: 4,315,819 (GRCm38)	H82Q	probably damaging	Het
Npbwr1	C	A	1: 5,916,658 (GRCm38)	L212F	probably damaging	Het
Nptx1	T	G	11: 119,543,316 (GRCm38)	I315L	probably benign	Het
Npy2r	G	T	3: 82,541,354 (GRCm38)	P38Q	possibly damaging	Het
Ocln	A	T	13: 100,535,029 (GRCm38)	D24E	probably damaging	Het
Or14c41	T	A	7: 86,585,884 (GRCm38)	V203D	possibly damaging	Het
Or5bw2	A	T	7: 6,570,023 (GRCm38)	I11F	probably damaging	Het
Or8d1b	T	C	9: 38,976,000 (GRCm38)	V108A	probably benign	Het
Pde2a	A	T	7: 101,484,567 (GRCm38)	D85V	probably damaging	Het
Phf3	A	G	1: 30,804,343 (GRCm38)	V1845A	probably benign	Het
Plch1	T	A	3: 63,697,977 (GRCm38)	Q1493L	possibly damaging	Het
Pold1	T	C	7: 44,541,484 (GRCm38)		probably benign	Het
Polq	T	C	16: 37,062,097 (GRCm38)	V1541A	probably benign	Het
Psmd3	T	A	11: 98,690,964 (GRCm38)	M305K	probably benign	Het
Pura	T	C	18: 36,287,750 (GRCm38)	F197L	possibly damaging	Het
Rab3gap2	T	A	1: 185,221,859 (GRCm38)	W43R	probably damaging	Het
Repin1	A	G	6: 48,596,530 (GRCm38)	Q128R	probably benign	Het
Rnf208	G	A	2: 25,243,644 (GRCm38)	V117I	probably damaging	Het
Rpe65	A	G	3: 159,615,571 (GRCm38)	Y340C	probably damaging	Het
Ryr1	C	A	7: 29,019,741 (GRCm38)	V4414L	unknown	Het
Sephs2	T	C	7: 127,273,477 (GRCm38)	E148G	possibly damaging	Het
Spns2	T	A	11: 72,457,268 (GRCm38)	Q291L	probably benign	Het
Ssc5d	C	T	7: 4,943,916 (GRCm38)	P1090S	probably benign	Het
Tasp1	A	T	2: 139,951,506 (GRCm38)	V250D	probably damaging	Het
Tcaf3	A	G	6: 42,591,430 (GRCm38)	I664T	possibly damaging	Het
Tg	T	C	15: 66,683,898 (GRCm38)	V813A	probably benign	Het
Tmem132c	T	C	5: 127,504,924 (GRCm38)	L401P	probably benign	Het
Tmem138	T	C	19: 10,571,603 (GRCm38)	N101S	probably benign	Het
Tmem242	G	T	17: 5,433,470 (GRCm38)	A99E	probably damaging	Het
Tmem30a	C	T	9: 79,774,164 (GRCm38)	R277H	probably benign	Het
Tmt1a	A	T	15: 100,313,168 (GRCm38)	I174F	probably benign	Het
Tnni3	T	A	7: 4,519,406 (GRCm38)	I182F	probably benign	Het
Trim30a	T	A	7: 104,411,504 (GRCm38)	D355V	probably damaging	Het
Trim35	C	T	14: 66,309,262 (GRCm38)	R493*	probably null	Het
Trip10	G	C	17: 57,255,135 (GRCm38)	V254L	probably benign	Het
Tshz2	A	T	2: 169,886,406 (GRCm38)	Q505L	probably benign	Het
Ttyh1	T	C	7: 4,128,184 (GRCm38)	V218A	probably damaging	Het
Unc45b	T	A	11: 82,917,799 (GRCm38)	M237K	probably benign	Het
Usp8	A	G	2: 126,758,568 (GRCm38)	T1080A	probably benign	Het
Vmn2r65	T	A	7: 84,940,911 (GRCm38)	H599L	possibly damaging	Het
Vps13c	A	G	9: 67,953,860 (GRCm38)	N2891S	probably benign	Het
Xpo5	C	T	17: 46,240,896 (GRCm38)	Q1050*	probably null	Het
Zfp407	G	T	18: 84,209,793 (GRCm38)	T1897K	probably damaging	Het
Zzef1	C	T	11: 72,900,633 (GRCm38)	R2188*	probably null	Het

Other mutations in Unc13b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Unc13b	APN	4	43,240,285 (GRCm38)	missense	probably damaging	1.00
IGL00832:Unc13b	APN	4	43,258,921 (GRCm38)	missense	probably damaging	1.00
IGL01111:Unc13b	APN	4	43,096,927 (GRCm38)	missense	possibly damaging	0.76
IGL01115:Unc13b	APN	4	43,258,492 (GRCm38)	missense	probably damaging	1.00
IGL01137:Unc13b	APN	4	43,091,291 (GRCm38)	missense	probably damaging	1.00
IGL01637:Unc13b	APN	4	43,241,066 (GRCm38)	missense	probably damaging	1.00
IGL01789:Unc13b	APN	4	43,239,462 (GRCm38)	missense	probably damaging	1.00
IGL01792:Unc13b	APN	4	43,250,218 (GRCm38)	missense	probably damaging	0.99
IGL01877:Unc13b	APN	4	43,249,583 (GRCm38)	critical splice donor site	probably null
IGL01924:Unc13b	APN	4	43,239,385 (GRCm38)	nonsense	probably null
IGL02087:Unc13b	APN	4	43,091,270 (GRCm38)	missense	probably null	1.00
IGL02197:Unc13b	APN	4	43,165,828 (GRCm38)	missense	probably damaging	0.99
IGL02504:Unc13b	APN	4	43,263,031 (GRCm38)	missense	probably damaging	1.00
IGL02659:Unc13b	APN	4	43,235,332 (GRCm38)	missense	probably damaging	1.00
IGL03031:Unc13b	APN	4	43,235,368 (GRCm38)	missense	probably damaging	1.00
IGL03036:Unc13b	APN	4	43,235,249 (GRCm38)	missense	probably damaging	1.00
IGL03209:Unc13b	APN	4	43,239,351 (GRCm38)	missense	probably damaging	0.99
IGL03352:Unc13b	APN	4	43,237,110 (GRCm38)	missense	possibly damaging	0.90
BB006:Unc13b	UTSW	4	43,174,399 (GRCm38)	missense	unknown
BB016:Unc13b	UTSW	4	43,174,399 (GRCm38)	missense	unknown
G1Funyon:Unc13b	UTSW	4	43,263,568 (GRCm38)	missense	probably benign
P0028:Unc13b	UTSW	4	43,256,225 (GRCm38)	missense	probably damaging	1.00
PIT4585001:Unc13b	UTSW	4	43,091,298 (GRCm38)	missense	probably benign	0.03
R0019:Unc13b	UTSW	4	43,096,990 (GRCm38)	missense	possibly damaging	0.58
R0019:Unc13b	UTSW	4	43,096,990 (GRCm38)	missense	possibly damaging	0.58
R0335:Unc13b	UTSW	4	43,236,983 (GRCm38)	missense	possibly damaging	0.95
R0504:Unc13b	UTSW	4	43,263,559 (GRCm38)	missense	probably damaging	0.99
R0631:Unc13b	UTSW	4	43,182,849 (GRCm38)	missense	possibly damaging	0.47
R0748:Unc13b	UTSW	4	43,241,164 (GRCm38)	splice site	probably benign
R1275:Unc13b	UTSW	4	43,235,366 (GRCm38)	missense	probably damaging	1.00
R1293:Unc13b	UTSW	4	43,235,190 (GRCm38)	missense	probably damaging	1.00
R1434:Unc13b	UTSW	4	43,239,385 (GRCm38)	nonsense	probably null
R1552:Unc13b	UTSW	4	43,237,144 (GRCm38)	missense	probably damaging	0.99
R1591:Unc13b	UTSW	4	43,244,747 (GRCm38)	missense	probably damaging	1.00
R1628:Unc13b	UTSW	4	43,263,371 (GRCm38)	missense	probably damaging	1.00
R1740:Unc13b	UTSW	4	43,240,285 (GRCm38)	missense	probably damaging	1.00
R1839:Unc13b	UTSW	4	43,258,308 (GRCm38)	splice site	probably benign
R2045:Unc13b	UTSW	4	43,091,266 (GRCm38)	missense	probably damaging	1.00
R2191:Unc13b	UTSW	4	43,245,566 (GRCm38)	nonsense	probably null
R2307:Unc13b	UTSW	4	43,239,854 (GRCm38)	missense	probably damaging	0.98
R2317:Unc13b	UTSW	4	43,245,514 (GRCm38)	missense	probably damaging	1.00
R2402:Unc13b	UTSW	4	43,095,843 (GRCm38)	missense	probably benign
R2847:Unc13b	UTSW	4	43,180,404 (GRCm38)	missense	probably benign	0.04
R3414:Unc13b	UTSW	4	43,234,658 (GRCm38)	splice site	probably benign
R3436:Unc13b	UTSW	4	43,097,028 (GRCm38)	splice site	probably benign
R3955:Unc13b	UTSW	4	43,256,834 (GRCm38)	missense	probably damaging	1.00
R3957:Unc13b	UTSW	4	43,256,834 (GRCm38)	missense	probably damaging	1.00
R4015:Unc13b	UTSW	4	43,237,801 (GRCm38)	missense	probably damaging	1.00
R4650:Unc13b	UTSW	4	43,261,035 (GRCm38)	missense	probably damaging	0.97
R4836:Unc13b	UTSW	4	43,237,137 (GRCm38)	missense	probably damaging	1.00
R5041:Unc13b	UTSW	4	43,237,836 (GRCm38)	missense	probably benign	0.41
R5413:Unc13b	UTSW	4	43,257,936 (GRCm38)	critical splice donor site	probably null
R5994:Unc13b	UTSW	4	43,172,596 (GRCm38)	intron	probably benign
R6015:Unc13b	UTSW	4	43,177,995 (GRCm38)	nonsense	probably null
R6090:Unc13b	UTSW	4	43,239,306 (GRCm38)	missense	probably damaging	1.00
R6242:Unc13b	UTSW	4	43,165,800 (GRCm38)	missense	possibly damaging	0.92
R6246:Unc13b	UTSW	4	43,216,246 (GRCm38)	missense	probably benign	0.18
R6427:Unc13b	UTSW	4	43,176,966 (GRCm38)	unclassified	probably benign
R6660:Unc13b	UTSW	4	43,177,412 (GRCm38)	unclassified	probably benign
R6670:Unc13b	UTSW	4	43,255,562 (GRCm38)	missense	probably damaging	0.99
R6753:Unc13b	UTSW	4	43,239,331 (GRCm38)	missense	probably damaging	1.00
R6858:Unc13b	UTSW	4	43,165,828 (GRCm38)	missense	possibly damaging	0.85
R6886:Unc13b	UTSW	4	43,170,156 (GRCm38)	intron	probably benign
R6969:Unc13b	UTSW	4	43,263,538 (GRCm38)	missense	possibly damaging	0.94
R6994:Unc13b	UTSW	4	43,173,203 (GRCm38)	intron	probably benign
R6994:Unc13b	UTSW	4	43,171,403 (GRCm38)	intron	probably benign
R7080:Unc13b	UTSW	4	43,171,926 (GRCm38)	missense	unknown
R7117:Unc13b	UTSW	4	43,216,544 (GRCm38)	missense	probably benign	0.33
R7132:Unc13b	UTSW	4	43,215,757 (GRCm38)	missense	probably benign	0.17
R7181:Unc13b	UTSW	4	43,258,893 (GRCm38)	missense	probably damaging	0.99
R7192:Unc13b	UTSW	4	43,258,519 (GRCm38)	missense	probably damaging	1.00
R7246:Unc13b	UTSW	4	43,172,910 (GRCm38)	missense	unknown
R7342:Unc13b	UTSW	4	43,258,703 (GRCm38)	missense	probably damaging	0.99
R7345:Unc13b	UTSW	4	43,173,966 (GRCm38)	missense	unknown
R7355:Unc13b	UTSW	4	43,237,754 (GRCm38)	missense	probably damaging	1.00
R7391:Unc13b	UTSW	4	43,216,459 (GRCm38)	missense	probably benign	0.03
R7419:Unc13b	UTSW	4	43,174,023 (GRCm38)	missense	unknown
R7424:Unc13b	UTSW	4	43,172,235 (GRCm38)	missense	unknown
R7517:Unc13b	UTSW	4	43,215,765 (GRCm38)	missense	probably benign
R7532:Unc13b	UTSW	4	43,249,565 (GRCm38)	missense	probably benign	0.44
R7564:Unc13b	UTSW	4	43,091,258 (GRCm38)	missense	probably damaging	1.00
R7598:Unc13b	UTSW	4	43,263,569 (GRCm38)	missense	probably benign	0.20
R7604:Unc13b	UTSW	4	43,256,776 (GRCm38)	missense	possibly damaging	0.95
R7604:Unc13b	UTSW	4	43,170,102 (GRCm38)	missense	unknown
R7643:Unc13b	UTSW	4	43,216,333 (GRCm38)	missense	probably benign
R7718:Unc13b	UTSW	4	43,173,854 (GRCm38)	missense	unknown
R7735:Unc13b	UTSW	4	43,165,791 (GRCm38)	missense	probably damaging	1.00
R7756:Unc13b	UTSW	4	43,177,312 (GRCm38)	small deletion	probably benign
R7757:Unc13b	UTSW	4	43,177,341 (GRCm38)	small insertion	probably benign
R7757:Unc13b	UTSW	4	43,177,330 (GRCm38)	small insertion	probably benign
R7757:Unc13b	UTSW	4	43,177,312 (GRCm38)	small deletion	probably benign
R7758:Unc13b	UTSW	4	43,177,344 (GRCm38)	small insertion	probably benign
R7758:Unc13b	UTSW	4	43,177,312 (GRCm38)	small insertion	probably benign
R7781:Unc13b	UTSW	4	43,259,546 (GRCm38)	missense	possibly damaging	0.87
R7793:Unc13b	UTSW	4	43,172,737 (GRCm38)	missense	unknown
R7858:Unc13b	UTSW	4	43,176,285 (GRCm38)	missense	unknown
R7867:Unc13b	UTSW	4	43,232,573 (GRCm38)	nonsense	probably null
R7897:Unc13b	UTSW	4	43,171,860 (GRCm38)	missense	unknown
R7904:Unc13b	UTSW	4	43,217,075 (GRCm38)	missense	probably benign
R7929:Unc13b	UTSW	4	43,174,399 (GRCm38)	missense	unknown
R7984:Unc13b	UTSW	4	43,173,973 (GRCm38)	missense	unknown
R8069:Unc13b	UTSW	4	43,177,597 (GRCm38)	missense	unknown
R8101:Unc13b	UTSW	4	43,239,918 (GRCm38)	missense	probably benign	0.08
R8246:Unc13b	UTSW	4	43,175,954 (GRCm38)	missense	unknown
R8289:Unc13b	UTSW	4	43,172,524 (GRCm38)	nonsense	probably null
R8301:Unc13b	UTSW	4	43,263,568 (GRCm38)	missense	probably benign
R8397:Unc13b	UTSW	4	43,217,290 (GRCm38)	missense	probably benign	0.12
R8421:Unc13b	UTSW	4	43,178,304 (GRCm38)	missense	unknown
R8738:Unc13b	UTSW	4	43,177,564 (GRCm38)	missense	unknown
R8746:Unc13b	UTSW	4	43,176,120 (GRCm38)	missense	unknown
R8766:Unc13b	UTSW	4	43,174,722 (GRCm38)	missense	unknown
R8825:Unc13b	UTSW	4	43,237,683 (GRCm38)	splice site	probably benign
R8834:Unc13b	UTSW	4	43,175,954 (GRCm38)	missense	unknown
R8862:Unc13b	UTSW	4	43,235,207 (GRCm38)	missense	probably damaging	1.00
R8864:Unc13b	UTSW	4	43,174,724 (GRCm38)	missense	unknown
R8889:Unc13b	UTSW	4	43,176,484 (GRCm38)	missense	unknown
R8892:Unc13b	UTSW	4	43,176,484 (GRCm38)	missense	unknown
R8904:Unc13b	UTSW	4	43,178,531 (GRCm38)	intron	probably benign
R9089:Unc13b	UTSW	4	43,095,847 (GRCm38)	missense	probably damaging	1.00
R9144:Unc13b	UTSW	4	43,173,649 (GRCm38)	missense	unknown
R9149:Unc13b	UTSW	4	43,176,186 (GRCm38)	missense	unknown
R9173:Unc13b	UTSW	4	43,177,421 (GRCm38)	missense	unknown
R9200:Unc13b	UTSW	4	43,257,352 (GRCm38)	missense	possibly damaging	0.50
R9232:Unc13b	UTSW	4	43,240,321 (GRCm38)	missense	probably benign	0.03
R9269:Unc13b	UTSW	4	43,171,955 (GRCm38)	missense	unknown
R9320:Unc13b	UTSW	4	43,171,044 (GRCm38)	missense	unknown
R9335:Unc13b	UTSW	4	43,255,551 (GRCm38)	missense	probably damaging	0.99
R9335:Unc13b	UTSW	4	43,216,123 (GRCm38)	missense	possibly damaging	0.86
R9352:Unc13b	UTSW	4	43,177,313 (GRCm38)	nonsense	probably null
R9352:Unc13b	UTSW	4	43,177,312 (GRCm38)	small insertion	probably benign
R9378:Unc13b	UTSW	4	43,173,282 (GRCm38)	missense	unknown
R9382:Unc13b	UTSW	4	43,172,512 (GRCm38)	missense	unknown
R9569:Unc13b	UTSW	4	43,177,312 (GRCm38)	small deletion	probably benign
R9622:Unc13b	UTSW	4	43,172,513 (GRCm38)	missense
R9687:Unc13b	UTSW	4	43,174,920 (GRCm38)	missense	unknown
R9704:Unc13b	UTSW	4	43,237,102 (GRCm38)	missense	probably benign	0.31
R9721:Unc13b	UTSW	4	43,101,869 (GRCm38)	missense	probably benign
R9753:Unc13b	UTSW	4	43,182,842 (GRCm38)	nonsense	probably null
RF016:Unc13b	UTSW	4	43,177,350 (GRCm38)	small insertion	probably benign
RF016:Unc13b	UTSW	4	43,177,347 (GRCm38)	small insertion	probably benign
RF041:Unc13b	UTSW	4	43,177,338 (GRCm38)	small insertion	probably benign
RF056:Unc13b	UTSW	4	43,177,359 (GRCm38)	small insertion	probably benign
Z1176:Unc13b	UTSW	4	43,177,764 (GRCm38)	missense	unknown
Z1176:Unc13b	UTSW	4	43,177,191 (GRCm38)	missense	unknown
Z1176:Unc13b	UTSW	4	43,171,419 (GRCm38)	missense	unknown
Z1176:Unc13b	UTSW	4	43,261,043 (GRCm38)	missense	probably benign	0.11
Z1177:Unc13b	UTSW	4	43,173,669 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CGATCTGTGACTTCTGCACTTG -3'
(R):5'- GCACGCTGCAAGAAGATGAC -3'

Sequencing Primer
(F):5'- ACTTGATGCTGACCCAGGC -3'
(R):5'- CGCTGCAAGAAGATGACTAATTACTG -3'

Posted On

2014-10-16