Mutagenetix > Incidental Mutations

Incidental Mutation 'R2263:Vmn2r63'

243921

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

040263-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.083) question?

Stock #

R2263 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42903251-42933789 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 42928607 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Asparagine at position 169 (T169N)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

Predicted Effect

probably benign
Transcript: ENSMUST00000163803
AA Change: T169N

PolyPhen 2 Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: T169N

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.5%

Validation Efficiency

98% (57/58)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932431P20Rik	T	C	7: 29,532,562		noncoding transcript	Het
9130011E15Rik	A	G	19: 45,932,349		probably null	Het
Abca13	T	C	11: 9,274,702	S472P	probably benign	Het
Arsi	T	C	18: 60,916,665	Y207H	probably damaging	Het
Atp2b4	C	T	1: 133,726,533	R778Q	probably benign	Het
Bdp1	T	A	13: 100,066,037	D693V	probably damaging	Het
Car12	C	A	9: 66,747,631	Y114*	probably null	Het
Col1a2	G	A	6: 4,518,822		probably benign	Het
Col4a1	A	G	8: 11,312,586		probably benign	Het
Cryl1	G	T	14: 57,286,408	Y232*	probably null	Het
Dnah11	T	A	12: 117,880,025	M4362L	probably benign	Het
Dnah11	T	C	12: 117,966,639	T3324A	probably damaging	Het
Dnah8	A	G	17: 30,673,835	N798S	probably damaging	Het
Dok4	A	T	8: 94,866,512	C182S	probably damaging	Het
Ehbp1	A	T	11: 22,095,462	N737K	probably benign	Het
Eif2b4	T	C	5: 31,192,574		probably benign	Het
Fat4	T	C	3: 38,888,989	V677A	possibly damaging	Het
Fbn2	A	G	18: 58,095,176		probably benign	Het
Fbxl3	G	T	14: 103,083,212	Y314*	probably null	Het
Fgf12	T	A	16: 28,189,611	K84*	probably null	Het
Flt3	G	A	5: 147,348,063	P748L	probably benign	Het
Folh1	C	T	7: 86,719,765	S685N	probably benign	Het
Gm13119	A	G	4: 144,363,541	I384V	probably benign	Het
Gpr65	A	T	12: 98,275,235	N49I	probably damaging	Het
Hdac7	A	G	15: 97,810,851		probably null	Het
Heatr5a	A	T	12: 51,916,150	V989E	probably damaging	Het
Ikbkap	A	G	4: 56,755,298		probably null	Het
Kif12	A	G	4: 63,169,521	S242P	probably benign	Het
Ltbp3	G	A	19: 5,754,022	R854Q	probably benign	Het
Mia3	A	G	1: 183,334,793	Y295H	probably benign	Het
Nell1	C	T	7: 50,560,821	T494I	possibly damaging	Het
Nmur2	A	T	11: 56,029,561	V286E	probably damaging	Het
Olfr1378	G	A	11: 50,969,869	V284I	possibly damaging	Het
Olfr20	T	A	11: 73,354,305	L184Q	possibly damaging	Het
Olfr574	T	C	7: 102,949,257	F254S	probably damaging	Het
Pcdh1	A	T	18: 38,198,657	L431H	probably benign	Het
Prex1	A	T	2: 166,589,068		probably benign	Het
Prim1	T	A	10: 128,015,263	D5E	probably benign	Het
Rubcnl	A	T	14: 75,040,820	N348I	possibly damaging	Het
Shisa9	G	T	16: 11,984,767	A63S	possibly damaging	Het
Shpk	G	A	11: 73,206,493		probably benign	Het
Slx4ip	T	C	2: 137,044,015	I85T	probably damaging	Het
Sptbn4	T	A	7: 27,434,357	D77V	probably damaging	Het
Strn3	A	T	12: 51,643,223		probably null	Het
Synrg	T	C	11: 83,977,152	M61T	possibly damaging	Het
Tekt4	T	A	17: 25,476,511	S439R	possibly damaging	Het
Ticam1	A	T	17: 56,271,888	V69E	possibly damaging	Het
Trpv1	C	T	11: 73,241,682	A374V	probably damaging	Het
Ubqln3	A	T	7: 104,141,635	L416*	probably null	Het
Uqcrfs1	G	A	13: 30,541,107	S150L	probably benign	Het
Utp15	T	C	13: 98,257,925	N153S	probably benign	Het
Vps13b	T	A	15: 35,646,181	V1477E	probably benign	Het
Xpo1	T	A	11: 23,284,634		probably null	Het
Xpo5	A	G	17: 46,230,343	I769V	probably benign	Het
Zc3h14	A	T	12: 98,758,514	H252L	probably benign	Het
Zfp410	G	T	12: 84,323,020		probably null	Het
Zfp521	T	C	18: 13,846,240	E372G	possibly damaging	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42904119	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42903364	missense	probably benign
IGL02203:Vmn2r63	APN	7	42904008	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42926850	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42903454	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42929192	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42927944	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42903985	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42927090	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42903275	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42903618	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42933705	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42928528	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42928010	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42928035	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42929218	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42929218	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42927915	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42928591	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42904126	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42928211	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42928319	missense	probably benign
R1698:Vmn2r63	UTSW	7	42933614	missense	probably benign
R1753:Vmn2r63	UTSW	7	42928245	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42926873	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42933580	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42928607	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42903405	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42926982	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42928113	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42933826	unclassified	probably null
R4649:Vmn2r63	UTSW	7	42903690	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42903690	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42928120	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42926890	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42903978	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42903745	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42928277	missense	probably benign
R5400:Vmn2r63	UTSW	7	42928211	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42933680	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42929011	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42928635	intron	probably null
R6362:Vmn2r63	UTSW	7	42903297	missense	probably benign
R6706:Vmn2r63	UTSW	7	42928577	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42903271	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42928535	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42933590	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42926967	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42926982	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42925269	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42927042	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42928129	missense	possibly damaging	0.46
Z1088:Vmn2r63	UTSW	7	42928559	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- AGAATCTGAGTCCCTCTGGG -3'
(R):5'- CTTGATGACAGATCCACAATTCC -3'

Sequencing Primer
(F):5'- CCTCTGGGGTCATTTGGGAC -3'
(R):5'- TGATGACAGATCCACAATTCCATTAC -3'

Posted On

2014-10-16