Mutagenetix > Incidental Mutation

Incidental Mutation 'R2304:Mcrip1'

244532

Institutional Source

Beutler Lab

Gene Symbol

Mcrip1

Ensembl Gene

ENSMUSG00000061111

Gene Name

MAPK regulated corepressor interacting protein 1

Synonyms

Fam195b, BC003940

MMRRC Submission

040303-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R2304 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

120433714-120440553 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 120435519 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 39 (F39I)

Ref Sequence

ENSEMBL: ENSMUSP00000034913 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034913]

AlphaFold

Q3UGS4

Predicted Effect

probably damaging
Transcript: ENSMUST00000034913
AA Change: F39I

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000034913
Gene: ENSMUSG00000061111
AA Change: F39I

Domain	Start	End	E-Value	Type
Pfam:FAM195	4	94	3.3e-26	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135515

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam22	T	A	5: 8,142,366 (GRCm39)	R806S	probably damaging	Het
Ccdc187	T	C	2: 26,171,029 (GRCm39)	K483R	possibly damaging	Het
Dcdc5	T	A	2: 106,166,488 (GRCm39)		noncoding transcript	Het
Dvl1	C	T	4: 155,940,041 (GRCm39)	S391F	probably damaging	Het
Erg28	A	G	12: 85,862,937 (GRCm39)	L125P	probably damaging	Het
Gkn3	C	T	6: 87,360,507 (GRCm39)	A163T	probably damaging	Het
Gm5449	G	T	13: 53,679,899 (GRCm39)		noncoding transcript	Het
Grid2ip	T	C	5: 143,373,595 (GRCm39)	Y796H	probably damaging	Het
Or5w12	T	A	2: 87,502,238 (GRCm39)	I158L	probably benign	Het
Prss53	T	C	7: 127,487,479 (GRCm39)	N291D	probably benign	Het
Ptpru	A	T	4: 131,499,879 (GRCm39)	V1255D	probably damaging	Het
Rbl1	T	C	2: 156,989,551 (GRCm39)	T1023A	probably benign	Het
Rnaseh2b	T	C	14: 62,598,838 (GRCm39)	S188P	probably damaging	Het
Sh2d6	T	C	6: 72,497,542 (GRCm39)	E20G	probably damaging	Het
Slc13a5	A	G	11: 72,149,865 (GRCm39)	V172A	probably damaging	Het
Slco5a1	C	T	1: 12,949,486 (GRCm39)	G635S	probably damaging	Het
Sp8	G	T	12: 118,812,304 (GRCm39)	S53I	possibly damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Togaram1	G	T	12: 65,023,630 (GRCm39)		probably null	Het
Trip11	A	T	12: 101,865,236 (GRCm39)	F146I	possibly damaging	Het
Vmn1r184	T	C	7: 25,966,550 (GRCm39)	S99P	probably damaging	Het
Xpot	T	C	10: 121,447,488 (GRCm39)	I325V	probably benign	Het
Zfp786	A	G	6: 47,797,633 (GRCm39)	L435P	probably damaging	Het

Other mutations in Mcrip1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
devonian	UTSW	11	120,435,505 (GRCm39)	critical splice donor site	probably null
R0184:Mcrip1	UTSW	11	120,435,710 (GRCm39)	start codon destroyed	probably null	0.00
R2289:Mcrip1	UTSW	11	120,435,530 (GRCm39)	missense	probably damaging	1.00
R7185:Mcrip1	UTSW	11	120,435,505 (GRCm39)	critical splice donor site	probably null
R8811:Mcrip1	UTSW	11	120,435,605 (GRCm39)	missense	probably damaging	1.00
R9142:Mcrip1	UTSW	11	120,435,542 (GRCm39)	missense	probably damaging	1.00
R9237:Mcrip1	UTSW	11	120,435,542 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAAGACCATCTCCCAGATCCTG -3'
(R):5'- TCTCAGGTGAATGTGGGAGC -3'

Sequencing Primer
(F):5'- AGATCCTGCCTCTTCTGGG -3'
(R):5'- AATGTGGGAGCTGGGACTCC -3'

Posted On

2014-10-30