Mutagenetix > Incidental Mutation

Incidental Mutation 'R2308:Zfp352'

244671

Institutional Source

Beutler Lab

Gene Symbol

Zfp352

Ensembl Gene

ENSMUSG00000070902

Gene Name

zinc finger protein 352

Synonyms

2czf48

MMRRC Submission

040307-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2308 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

90218820-90225702 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 90225243 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 540 (K540R)

Ref Sequence

ENSEMBL: ENSMUSP00000102746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080541] [ENSMUST00000107129]

AlphaFold

A2AML7

Predicted Effect

probably benign
Transcript: ENSMUST00000080541
AA Change: K540R

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: K540R

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107129
AA Change: K540R

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: K540R

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Coding Region Coverage

1x: 99.4%
3x: 98.6%
10x: 96.9%
20x: 93.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Depdc1b	G	A	13: 108,373,841	V296I	possibly damaging	Het
Dot1l	T	C	10: 80,789,069	S907P	probably damaging	Het
Epha7	G	A	4: 28,821,503	E223K	possibly damaging	Het
Ercc6	T	C	14: 32,566,409	I846T	possibly damaging	Het
Exoc4	A	G	6: 33,918,568	Y840C	probably damaging	Het
Gramd1a	T	C	7: 31,139,790	D231G	probably damaging	Het
Mark2	A	C	19: 7,281,934	S90A	probably damaging	Het
Mbd1	A	G	18: 74,276,477	Q432R	probably benign	Het
Mcm2	G	A	6: 88,893,008	R60C	probably damaging	Het
Met	T	C	6: 17,491,742	S168P	probably benign	Het
Nup210	T	C	6: 91,040,868	I304V	probably benign	Het
Olfr1197	T	A	2: 88,729,084	I172F	probably damaging	Het
Olfr356	T	A	2: 36,937,300	Y60*	probably null	Het
Ppfia4	A	G	1: 134,332,397	S43P	possibly damaging	Het
Rbbp8	A	T	18: 11,696,776	K132I	possibly damaging	Het
Rpap1	G	A	2: 119,783,766	P50L	probably benign	Het
Slc41a3	T	A	6: 90,612,120	I71K	possibly damaging	Het
Spaca7	A	T	8: 12,598,959	N127I	probably benign	Het
Stkld1	A	T	2: 26,952,714	D566V	probably damaging	Het
Tbxas1	A	G	6: 39,027,661	M281V	probably benign	Het
Txnl1	T	C	18: 63,671,620	T268A	probably benign	Het
Unc80	A	T	1: 66,648,997	I2385F	possibly damaging	Het
Vmn2r118	A	G	17: 55,624,650	I8T	probably benign	Het
Vmn2r80	T	C	10: 79,171,621	F477S	probably damaging	Het
Ythdc2	A	G	18: 44,847,748	E470G	possibly damaging	Het

Other mutations in Zfp352

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01963:Zfp352	APN	4	90224154	missense	possibly damaging	0.95
IGL02252:Zfp352	APN	4	90224130	missense	probably benign	0.02
IGL03156:Zfp352	APN	4	90224087	missense	possibly damaging	0.57
IGL03167:Zfp352	APN	4	90224702	missense	probably damaging	0.99
IGL03190:Zfp352	APN	4	90223757	missense	possibly damaging	0.94
IGL03335:Zfp352	APN	4	90224346	missense	probably damaging	0.99
R0051:Zfp352	UTSW	4	90224285	missense	probably damaging	0.99
R0403:Zfp352	UTSW	4	90225009	missense	possibly damaging	0.60
R0550:Zfp352	UTSW	4	90224690	missense	probably damaging	0.99
R0671:Zfp352	UTSW	4	90223919	missense	probably benign
R1034:Zfp352	UTSW	4	90224156	missense	possibly damaging	0.94
R1754:Zfp352	UTSW	4	90223809	missense	probably benign	0.23
R2016:Zfp352	UTSW	4	90225171	missense	probably benign	0.42
R2064:Zfp352	UTSW	4	90225120	missense	probably benign	0.08
R3552:Zfp352	UTSW	4	90225102	missense	probably benign	0.33
R3794:Zfp352	UTSW	4	90225149	missense	probably damaging	1.00
R3795:Zfp352	UTSW	4	90225149	missense	probably damaging	1.00
R4135:Zfp352	UTSW	4	90225024	missense	probably damaging	0.96
R4356:Zfp352	UTSW	4	90223834	missense	possibly damaging	0.91
R4409:Zfp352	UTSW	4	90225164	missense	probably benign	0.00
R4590:Zfp352	UTSW	4	90224535	missense	probably damaging	0.98
R4614:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4617:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4618:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4741:Zfp352	UTSW	4	90224940	missense	possibly damaging	0.94
R4931:Zfp352	UTSW	4	90224304	missense	probably damaging	0.98
R4959:Zfp352	UTSW	4	90224139	missense	probably benign	0.01
R4973:Zfp352	UTSW	4	90224139	missense	probably benign	0.01
R5167:Zfp352	UTSW	4	90224216	missense	possibly damaging	0.94
R5260:Zfp352	UTSW	4	90224460	missense	probably damaging	0.99
R5524:Zfp352	UTSW	4	90225104	missense	possibly damaging	0.95
R5942:Zfp352	UTSW	4	90225070	missense	probably damaging	0.98
R6802:Zfp352	UTSW	4	90225200	missense	probably benign	0.33
R6819:Zfp352	UTSW	4	90224699	missense	probably benign
R7072:Zfp352	UTSW	4	90224424	missense	probably benign	0.00
R7099:Zfp352	UTSW	4	90224880	missense	probably benign	0.00
R7569:Zfp352	UTSW	4	90223659	missense	possibly damaging	0.77
R7645:Zfp352	UTSW	4	90224777	missense	probably benign	0.13
R7705:Zfp352	UTSW	4	90225275	missense	possibly damaging	0.94
R8424:Zfp352	UTSW	4	90224243	missense	possibly damaging	0.87
R9180:Zfp352	UTSW	4	90224881	missense	probably benign	0.38
R9378:Zfp352	UTSW	4	90224338	missense	probably benign	0.13
R9509:Zfp352	UTSW	4	90224706	missense	probably damaging	0.99
R9623:Zfp352	UTSW	4	90224891	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GTGTCACAGAAGGACCCTAGTC -3'
(R):5'- GCTCTGCACATTCTGGACAG -3'

Sequencing Primer
(F):5'- GACCCTAGTCCTTTGAAGCTC -3'
(R):5'- TCTGCACATTCTGGACAGATCAGG -3'

Posted On

2014-10-30