Incidental Mutation 'R2308:Zfp352'
| ID |
244671 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp352
|
| Ensembl Gene |
ENSMUSG00000070902 |
| Gene Name |
zinc finger protein 352 |
| Synonyms |
2czf48 |
| MMRRC Submission |
040307-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R2308 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
4 |
| Chromosomal Location |
90218820-90225702 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 90225243 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Lysine to Arginine
at position 540
(K540R)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000102746
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000080541]
[ENSMUST00000107129]
|
| AlphaFold |
A2AML7 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000080541
AA Change: K540R
PolyPhen 2
Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)
|
| SMART Domains |
Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: K540R
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000107129
AA Change: K540R
PolyPhen 2
Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)
|
| SMART Domains |
Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: K540R
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.4%
- 3x: 98.6%
- 10x: 96.9%
- 20x: 93.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 25 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Depdc1b |
G |
A |
13: 108,373,841 (GRCm38) |
V296I |
possibly damaging |
Het |
| Dot1l |
T |
C |
10: 80,789,069 (GRCm38) |
S907P |
probably damaging |
Het |
| Epha7 |
G |
A |
4: 28,821,503 (GRCm38) |
E223K |
possibly damaging |
Het |
| Ercc6 |
T |
C |
14: 32,566,409 (GRCm38) |
I846T |
possibly damaging |
Het |
| Exoc4 |
A |
G |
6: 33,918,568 (GRCm38) |
Y840C |
probably damaging |
Het |
| Gramd1a |
T |
C |
7: 31,139,790 (GRCm38) |
D231G |
probably damaging |
Het |
| Mark2 |
A |
C |
19: 7,281,934 (GRCm38) |
S90A |
probably damaging |
Het |
| Mbd1 |
A |
G |
18: 74,276,477 (GRCm38) |
Q432R |
probably benign |
Het |
| Mcm2 |
G |
A |
6: 88,893,008 (GRCm38) |
R60C |
probably damaging |
Het |
| Met |
T |
C |
6: 17,491,742 (GRCm38) |
S168P |
probably benign |
Het |
| Nup210 |
T |
C |
6: 91,040,868 (GRCm38) |
I304V |
probably benign |
Het |
| Olfr1197 |
T |
A |
2: 88,729,084 (GRCm38) |
I172F |
probably damaging |
Het |
| Olfr356 |
T |
A |
2: 36,937,300 (GRCm38) |
Y60* |
probably null |
Het |
| Ppfia4 |
A |
G |
1: 134,332,397 (GRCm38) |
S43P |
possibly damaging |
Het |
| Rbbp8 |
A |
T |
18: 11,696,776 (GRCm38) |
K132I |
possibly damaging |
Het |
| Rpap1 |
G |
A |
2: 119,783,766 (GRCm38) |
P50L |
probably benign |
Het |
| Slc41a3 |
T |
A |
6: 90,612,120 (GRCm38) |
I71K |
possibly damaging |
Het |
| Spaca7 |
A |
T |
8: 12,598,959 (GRCm38) |
N127I |
probably benign |
Het |
| Stkld1 |
A |
T |
2: 26,952,714 (GRCm38) |
D566V |
probably damaging |
Het |
| Tbxas1 |
A |
G |
6: 39,027,661 (GRCm38) |
M281V |
probably benign |
Het |
| Txnl1 |
T |
C |
18: 63,671,620 (GRCm38) |
T268A |
probably benign |
Het |
| Unc80 |
A |
T |
1: 66,648,997 (GRCm38) |
I2385F |
possibly damaging |
Het |
| Vmn2r118 |
A |
G |
17: 55,624,650 (GRCm38) |
I8T |
probably benign |
Het |
| Vmn2r80 |
T |
C |
10: 79,171,621 (GRCm38) |
F477S |
probably damaging |
Het |
| Ythdc2 |
A |
G |
18: 44,847,748 (GRCm38) |
E470G |
possibly damaging |
Het |
|
| Other mutations in Zfp352 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01963:Zfp352
|
APN |
4 |
90,224,154 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
IGL02252:Zfp352
|
APN |
4 |
90,224,130 (GRCm38) |
missense |
probably benign |
0.02 |
|
IGL03156:Zfp352
|
APN |
4 |
90,224,087 (GRCm38) |
missense |
possibly damaging |
0.57 |
|
IGL03167:Zfp352
|
APN |
4 |
90,224,702 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL03190:Zfp352
|
APN |
4 |
90,223,757 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
IGL03335:Zfp352
|
APN |
4 |
90,224,346 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0051:Zfp352
|
UTSW |
4 |
90,224,285 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0403:Zfp352
|
UTSW |
4 |
90,225,009 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R0550:Zfp352
|
UTSW |
4 |
90,224,690 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0671:Zfp352
|
UTSW |
4 |
90,223,919 (GRCm38) |
missense |
probably benign |
|
|
R1034:Zfp352
|
UTSW |
4 |
90,224,156 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R1754:Zfp352
|
UTSW |
4 |
90,223,809 (GRCm38) |
missense |
probably benign |
0.23 |
|
R2016:Zfp352
|
UTSW |
4 |
90,225,171 (GRCm38) |
missense |
probably benign |
0.42 |
|
R2064:Zfp352
|
UTSW |
4 |
90,225,120 (GRCm38) |
missense |
probably benign |
0.08 |
|
R3552:Zfp352
|
UTSW |
4 |
90,225,102 (GRCm38) |
missense |
probably benign |
0.33 |
|
R3794:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3795:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4135:Zfp352
|
UTSW |
4 |
90,225,024 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R4356:Zfp352
|
UTSW |
4 |
90,223,834 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
R4409:Zfp352
|
UTSW |
4 |
90,225,164 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4590:Zfp352
|
UTSW |
4 |
90,224,535 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4614:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4617:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4618:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4741:Zfp352
|
UTSW |
4 |
90,224,940 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R4931:Zfp352
|
UTSW |
4 |
90,224,304 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4959:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R4973:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5167:Zfp352
|
UTSW |
4 |
90,224,216 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5260:Zfp352
|
UTSW |
4 |
90,224,460 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R5524:Zfp352
|
UTSW |
4 |
90,225,104 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R5942:Zfp352
|
UTSW |
4 |
90,225,070 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6802:Zfp352
|
UTSW |
4 |
90,225,200 (GRCm38) |
missense |
probably benign |
0.33 |
|
R6819:Zfp352
|
UTSW |
4 |
90,224,699 (GRCm38) |
missense |
probably benign |
|
|
R7072:Zfp352
|
UTSW |
4 |
90,224,424 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7099:Zfp352
|
UTSW |
4 |
90,224,880 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7569:Zfp352
|
UTSW |
4 |
90,223,659 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R7645:Zfp352
|
UTSW |
4 |
90,224,777 (GRCm38) |
missense |
probably benign |
0.13 |
|
R7705:Zfp352
|
UTSW |
4 |
90,225,275 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R8424:Zfp352
|
UTSW |
4 |
90,224,243 (GRCm38) |
missense |
possibly damaging |
0.87 |
|
R9180:Zfp352
|
UTSW |
4 |
90,224,881 (GRCm38) |
missense |
probably benign |
0.38 |
|
R9378:Zfp352
|
UTSW |
4 |
90,224,338 (GRCm38) |
missense |
probably benign |
0.13 |
|
R9509:Zfp352
|
UTSW |
4 |
90,224,706 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9623:Zfp352
|
UTSW |
4 |
90,224,891 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- GTGTCACAGAAGGACCCTAGTC -3'
(R):5'- GCTCTGCACATTCTGGACAG -3'
Sequencing Primer
(F):5'- GACCCTAGTCCTTTGAAGCTC -3'
(R):5'- TCTGCACATTCTGGACAGATCAGG -3'
|
| Posted On |
2014-10-30 |
Incidental Mutation