Incidental Mutation 'R2367:Gstt2'
| ID |
246378 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gstt2
|
| Ensembl Gene |
ENSMUSG00000033318 |
| Gene Name |
glutathione S-transferase, theta 2 |
| Synonyms |
Yrs, mGSTT2 |
| MMRRC Submission |
040348-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R2367 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
10 |
| Chromosomal Location |
75667676-75673253 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to T
at 75668524 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glycine to Serine
at position 108
(G108S)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000151307
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000038257]
[ENSMUST00000218500]
[ENSMUST00000220195]
[ENSMUST00000220440]
|
| AlphaFold |
Q61133 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000038257
AA Change: G108S
PolyPhen 2
Score 0.152 (Sensitivity: 0.92; Specificity: 0.87)
|
| SMART Domains |
Protein: ENSMUSP00000046324
Gene: ENSMUSG00000033318
AA Change: G108S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:GST_N
|
1 |
76 |
4.6e-12 |
PFAM |
|
Pfam:GST_N_3
|
5 |
84 |
1.4e-11 |
PFAM |
|
Pfam:GST_N_2
|
11 |
77 |
3.5e-10 |
PFAM |
|
Pfam:GST_C
|
73 |
199 |
1.5e-10 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000218034
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000218500
AA Change: G108S
PolyPhen 2
Score 0.435 (Sensitivity: 0.89; Specificity: 0.90)
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000218646
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000218745
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000219343
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000220195
AA Change: G115S
PolyPhen 2
Score 0.039 (Sensitivity: 0.94; Specificity: 0.83)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000220440
AA Change: G108S
PolyPhen 2
Score 0.152 (Sensitivity: 0.92; Specificity: 0.87)
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.3%
- 20x: 95.2%
|
| Validation Efficiency |
|
| MGI Phenotype |
PHENOTYPE: Mice homozygous for a disruption in this gene display a normal phenotype. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 33 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adipoq |
T |
C |
16: 22,974,069 (GRCm39) |
V32A |
probably benign |
Het |
| Ceacam3 |
T |
C |
7: 16,885,813 (GRCm39) |
|
probably null |
Het |
| Cep250 |
G |
A |
2: 155,834,552 (GRCm39) |
R2159K |
probably damaging |
Het |
| Cfap46 |
C |
A |
7: 139,233,414 (GRCm39) |
V296F |
probably damaging |
Het |
| Ctdspl2 |
T |
C |
2: 121,817,499 (GRCm39) |
V182A |
probably benign |
Het |
| Cyp2c69 |
T |
C |
19: 39,866,038 (GRCm39) |
N185S |
probably benign |
Het |
| Daxx |
C |
T |
17: 34,130,821 (GRCm39) |
R279W |
probably benign |
Het |
| Dnajc28 |
G |
A |
16: 91,413,755 (GRCm39) |
T187M |
probably damaging |
Het |
| Fat3 |
G |
A |
9: 15,909,567 (GRCm39) |
S2145F |
probably damaging |
Het |
| Galnt13 |
A |
T |
2: 55,002,956 (GRCm39) |
M552L |
probably benign |
Het |
| Heatr1 |
T |
A |
13: 12,448,605 (GRCm39) |
L422Q |
probably damaging |
Het |
| Kntc1 |
C |
T |
5: 123,919,255 (GRCm39) |
L845F |
probably damaging |
Het |
| Krtap26-1 |
C |
A |
16: 88,444,213 (GRCm39) |
R136L |
probably benign |
Het |
| Mrgprb5 |
C |
T |
7: 47,818,347 (GRCm39) |
W129* |
probably null |
Het |
| Ms4a3 |
G |
A |
19: 11,607,108 (GRCm39) |
P186S |
probably benign |
Het |
| Myo15a |
T |
A |
11: 60,408,064 (GRCm39) |
M3184K |
probably damaging |
Het |
| Olig2 |
G |
A |
16: 91,023,454 (GRCm39) |
R56Q |
possibly damaging |
Het |
| Or8u9 |
T |
C |
2: 86,001,981 (GRCm39) |
Y60C |
probably damaging |
Het |
| Pgap3 |
C |
T |
11: 98,281,985 (GRCm39) |
|
probably null |
Het |
| Phgdh |
C |
G |
3: 98,221,612 (GRCm39) |
G440A |
probably benign |
Het |
| Scn1a |
T |
C |
2: 66,158,023 (GRCm39) |
Q450R |
probably damaging |
Het |
| Sema4d |
CGGGG |
CGGGGGGG |
13: 51,857,176 (GRCm39) |
|
probably benign |
Het |
| Sik1 |
C |
T |
17: 32,065,271 (GRCm39) |
V776I |
possibly damaging |
Het |
| Slc25a39 |
T |
A |
11: 102,294,477 (GRCm39) |
T316S |
possibly damaging |
Het |
| Slc38a10 |
C |
T |
11: 120,001,087 (GRCm39) |
E578K |
probably benign |
Het |
| Slc44a5 |
T |
A |
3: 153,953,446 (GRCm39) |
I276K |
possibly damaging |
Het |
| Smarcc2 |
A |
G |
10: 128,318,036 (GRCm39) |
T610A |
possibly damaging |
Het |
| Spag9 |
G |
A |
11: 94,007,583 (GRCm39) |
S1090N |
probably damaging |
Het |
| Tpr |
T |
C |
1: 150,309,479 (GRCm39) |
V277A |
probably damaging |
Het |
| Traf3ip1 |
T |
G |
1: 91,435,242 (GRCm39) |
S314A |
possibly damaging |
Het |
| Tril |
T |
A |
6: 53,796,151 (GRCm39) |
D357V |
probably damaging |
Het |
| Tube1 |
T |
C |
10: 39,020,915 (GRCm39) |
L267P |
probably damaging |
Het |
| Vmn1r11 |
A |
G |
6: 57,114,416 (GRCm39) |
I27V |
probably benign |
Het |
|
| Other mutations in Gstt2 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01571:Gstt2
|
APN |
10 |
75,670,005 (GRCm39) |
splice site |
probably benign |
|
|
IGL01710:Gstt2
|
APN |
10 |
75,669,579 (GRCm39) |
splice site |
probably benign |
|
|
IGL02642:Gstt2
|
APN |
10 |
75,668,652 (GRCm39) |
missense |
probably benign |
0.17 |
|
IGL03375:Gstt2
|
APN |
10 |
75,668,655 (GRCm39) |
splice site |
probably null |
|
|
R0389:Gstt2
|
UTSW |
10 |
75,668,266 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1751:Gstt2
|
UTSW |
10 |
75,670,098 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1767:Gstt2
|
UTSW |
10 |
75,670,098 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1971:Gstt2
|
UTSW |
10 |
75,668,499 (GRCm39) |
missense |
probably benign |
0.10 |
|
R5027:Gstt2
|
UTSW |
10 |
75,667,726 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7457:Gstt2
|
UTSW |
10 |
75,668,354 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7579:Gstt2
|
UTSW |
10 |
75,670,019 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7910:Gstt2
|
UTSW |
10 |
75,667,736 (GRCm39) |
missense |
probably benign |
0.25 |
|
R7912:Gstt2
|
UTSW |
10 |
75,668,418 (GRCm39) |
missense |
probably benign |
0.27 |
|
R8282:Gstt2
|
UTSW |
10 |
75,668,291 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8348:Gstt2
|
UTSW |
10 |
75,668,526 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8448:Gstt2
|
UTSW |
10 |
75,668,526 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8488:Gstt2
|
UTSW |
10 |
75,667,823 (GRCm39) |
missense |
possibly damaging |
0.69 |
|
R9259:Gstt2
|
UTSW |
10 |
75,669,511 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
R9261:Gstt2
|
UTSW |
10 |
75,669,511 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
| Predicted Primers |
PCR Primer
(F):5'- GGAACTTGTCCTCCAGCTGTTG -3'
(R):5'- GCCAAGGTCCTTAAATCCCC -3'
Sequencing Primer
(F):5'- TGTTGCAGAACCAGGACCATTCTATC -3'
(R):5'- CTCTTCCCATGGTCCATATAGC -3'
|
| Posted On |
2014-10-30 |
Incidental Mutation