Mutagenetix > Incidental Mutation

Incidental Mutation 'R2332:Gm5444'

246478

Institutional Source

Beutler Lab

Gene Symbol

Gm5444

Ensembl Gene

ENSMUSG00000053499

Gene Name

predicted gene 5444

Synonyms

MMRRC Submission

040322-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R2332 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

4821650-4886484 bp(+) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

A to T at 4883624 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000065956

SMART Domains

Protein: ENSMUSP00000070493
Gene: ENSMUSG00000053499

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
low complexity region	83	103	N/A	INTRINSIC

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

98% (47/48)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apc	T	C	18: 34,450,112 (GRCm39)	I2302T	possibly damaging	Het
Apoa4	G	A	9: 46,153,653 (GRCm39)	V85I	probably benign	Het
Banf1	C	T	19: 5,415,058 (GRCm39)	W84*	probably null	Het
Cdk13	A	G	13: 17,893,280 (GRCm39)	L627P	probably damaging	Het
Cep250	A	G	2: 155,832,527 (GRCm39)	E1483G	probably damaging	Het
Crygs	C	T	16: 22,624,301 (GRCm39)	G102D	possibly damaging	Het
Ddx60	A	G	8: 62,490,125 (GRCm39)	E1698G	probably benign	Het
Depdc1a	C	A	3: 159,229,503 (GRCm39)	Q612K	probably damaging	Het
Dnaja2	G	A	8: 86,266,765 (GRCm39)	R321C	probably damaging	Het
Fam186b	T	A	15: 99,178,309 (GRCm39)	E339V	probably benign	Het
Fga	T	C	3: 82,938,704 (GRCm39)	F360L	probably damaging	Het
Fut9	C	T	4: 25,619,823 (GRCm39)	W330*	probably null	Het
Ghr	T	C	15: 3,349,891 (GRCm39)	N429S	probably benign	Het
Hjurp	G	C	1: 88,204,937 (GRCm39)		probably benign	Het
Hoxa5	T	C	6: 52,179,659 (GRCm39)	I239V	probably damaging	Het
Hps6	T	C	19: 45,992,930 (GRCm39)	V289A	possibly damaging	Het
Iqcb1	A	G	16: 36,663,801 (GRCm39)	N190D	possibly damaging	Het
Map3k13	G	A	16: 21,717,427 (GRCm39)		probably null	Het
Or10ag53	T	C	2: 87,083,217 (GRCm39)	V312A	possibly damaging	Het
Or52d13	A	G	7: 103,110,293 (GRCm39)	Y41H	probably damaging	Het
Pacsin1	A	G	17: 27,923,885 (GRCm39)	E93G	possibly damaging	Het
Pds5a	A	G	5: 65,784,422 (GRCm39)		probably null	Het
Ppp1r9b	A	T	11: 94,887,435 (GRCm39)	E482D	probably damaging	Het
Rhobtb3	A	G	13: 76,058,971 (GRCm39)	S276P	probably benign	Het
Rimbp2	A	G	5: 128,866,705 (GRCm39)	V538A	probably benign	Het
Rmdn3	A	T	2: 118,984,008 (GRCm39)		probably benign	Het
Setx	GTGGCT	GT	2: 29,044,073 (GRCm39)	1814	probably null	Het
Sh3rf1	C	T	8: 61,679,321 (GRCm39)	P121L	probably benign	Het
Slc4a11	A	T	2: 130,526,379 (GRCm39)	V855D	probably benign	Het
Speer4f1	C	A	5: 17,684,522 (GRCm39)	N183K	probably damaging	Het
Sstr4	A	T	2: 148,238,330 (GRCm39)	N314Y	probably damaging	Het
Synj2	A	G	17: 6,074,069 (GRCm39)	K288E	probably damaging	Het
Trhde	T	A	10: 114,428,070 (GRCm39)	N409Y	probably damaging	Het
Ttn	A	T	2: 76,611,483 (GRCm39)	W15604R	probably damaging	Het
Ugdh	C	T	5: 65,584,827 (GRCm39)	V32I	possibly damaging	Het
Uhrf1	C	A	17: 56,617,671 (GRCm39)		probably null	Het
Vps13d	G	T	4: 144,875,256 (GRCm39)	D1750E	probably benign	Het
Wdfy3	A	G	5: 102,036,189 (GRCm39)		probably benign	Het
Wnt4	C	A	4: 137,023,831 (GRCm39)	T266K	probably benign	Het
Wtap	C	T	17: 13,186,425 (GRCm39)	R374Q	possibly damaging	Het
Zfp322a	A	T	13: 23,541,494 (GRCm39)	C83S	probably damaging	Het

Other mutations in Gm5444

Allele	Source	Chr	Coord	Type	Predicted Effect
R0716:Gm5444	UTSW	13	4,884,192 (GRCm39)	exon	noncoding transcript
R2341:Gm5444	UTSW	13	4,884,344 (GRCm39)	exon	noncoding transcript
R3901:Gm5444	UTSW	13	4,884,278 (GRCm39)	exon	noncoding transcript
R4520:Gm5444	UTSW	13	4,884,225 (GRCm39)	exon	noncoding transcript
R5148:Gm5444	UTSW	13	4,884,314 (GRCm39)	exon	noncoding transcript
R5856:Gm5444	UTSW	13	4,821,683 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- ATGGGAATTCATCTCTTCTACTACC -3'
(R):5'- CAGAAGATAAATGCCTGCTTGG -3'

Sequencing Primer
(F):5'- TGTAAAATTCCTAAAGTACCAATGGG -3'
(R):5'- CCTGCTTGGCTCTATAAAAA -3'

Posted On

2014-10-30