Mutagenetix > Incidental Mutation

Incidental Mutation 'R2358:Or10al5'

247039

Institutional Source

Beutler Lab

Gene Symbol

Or10al5

Ensembl Gene

ENSMUSG00000060017

Gene Name

olfactory receptor family 10 subfamily AL member 5

Synonyms

Olfr121, GA_x6K02T2PSCP-2211113-2212078, MOR263-4

MMRRC Submission

040340-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R2358 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38059757-38063895 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 38063271 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 175 (C175*)

Ref Sequence

ENSEMBL: ENSMUSP00000133865 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074555] [ENSMUST00000174675]

AlphaFold

Q5CZY0

Predicted Effect

probably null
Transcript: ENSMUST00000074555
AA Change: C175*

SMART Domains

Protein: ENSMUSP00000084377
Gene: ENSMUSG00000060017
AA Change: C175*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	314	2.9e-56	PFAM
Pfam:7TM_GPCR_Srsx	41	311	2.1e-5	PFAM
Pfam:7tm_1	47	296	8.3e-22	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000174675
AA Change: C175*

SMART Domains

Protein: ENSMUSP00000133865
Gene: ENSMUSG00000060017
AA Change: C175*

Domain	Start	End	E-Value	Type
transmembrane domain	33	55	N/A	INTRINSIC

Meta Mutation Damage Score

0.9666

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.1%

Validation Efficiency

98% (55/56)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb9	CGG	CG	5: 124,215,368 (GRCm39)		probably null	Het
Aif1l	T	A	2: 31,859,763 (GRCm39)	F94L	probably damaging	Het
Ankzf1	C	T	1: 75,171,895 (GRCm39)	H209Y	probably damaging	Het
Ate1	A	T	7: 130,117,895 (GRCm39)	M30K	probably damaging	Het
Cd27	A	G	6: 125,210,281 (GRCm39)	Y189H	probably damaging	Het
Cela1	A	G	15: 100,579,109 (GRCm39)	I183T	probably benign	Het
Copg2	A	T	6: 30,803,168 (GRCm39)	L259*	probably null	Het
Dennd2b	A	T	7: 109,155,653 (GRCm39)	S366T	probably benign	Het
Efcab7	T	A	4: 99,719,823 (GRCm39)		probably benign	Het
Fcrl5	A	G	3: 87,353,726 (GRCm39)	E357G	probably damaging	Het
Fzr1	C	T	10: 81,203,474 (GRCm39)		probably null	Het
Il12rb2	C	T	6: 67,275,179 (GRCm39)	A649T	probably damaging	Het
Itfg1	C	A	8: 86,464,758 (GRCm39)	V438F	probably damaging	Het
Jaml	A	C	9: 45,012,361 (GRCm39)	I283L	possibly damaging	Het
Kif28	A	T	1: 179,537,024 (GRCm39)	H486Q	probably damaging	Het
Lrch4	A	G	5: 137,636,810 (GRCm39)		probably benign	Het
Lrfn2	A	G	17: 49,378,188 (GRCm39)	E423G	possibly damaging	Het
Lrp4	T	A	2: 91,332,299 (GRCm39)	N1665K	probably benign	Het
Mrpl32	A	T	13: 14,785,165 (GRCm39)	V157E	probably damaging	Het
Mta3	A	G	17: 84,070,417 (GRCm39)	I193V	probably damaging	Het
Myom2	G	A	8: 15,162,018 (GRCm39)	V984I	possibly damaging	Het
Nedd4l	G	A	18: 65,342,790 (GRCm39)	V909I	possibly damaging	Het
Nlrp4a	A	G	7: 26,163,623 (GRCm39)	D930G	probably benign	Het
Or5d47	A	T	2: 87,804,066 (GRCm39)	N314K	probably benign	Het
Or7a35	T	C	10: 78,854,022 (GRCm39)	F289L	probably damaging	Het
Ovch2	A	T	7: 107,394,122 (GRCm39)	H110Q	probably damaging	Het
Pcnx3	G	A	19: 5,733,367 (GRCm39)	Q155*	probably null	Het
Pcnx3	C	G	19: 5,733,368 (GRCm39)	L1F	probably null	Het
Pi4k2a	G	A	19: 42,079,131 (GRCm39)	R64Q	probably damaging	Het
Ptpn12	G	A	5: 21,203,690 (GRCm39)	P363S	probably damaging	Het
Rbm27	T	C	18: 42,425,177 (GRCm39)		probably benign	Het
Ripor3	A	G	2: 167,825,785 (GRCm39)		probably benign	Het
Rpl13-ps3	A	G	14: 59,131,265 (GRCm39)		noncoding transcript	Het
Sap18b	G	A	8: 96,552,191 (GRCm39)	R67H	probably benign	Het
Sdhb	T	C	4: 140,700,311 (GRCm39)	V137A	probably damaging	Het
Shmt2	T	C	10: 127,353,897 (GRCm39)	T459A	probably benign	Het
Siglecg	A	G	7: 43,058,846 (GRCm39)	S200G	possibly damaging	Het
Slc6a15	T	G	10: 103,252,646 (GRCm39)	I603S	probably benign	Het
Smtn	A	T	11: 3,482,865 (GRCm39)		probably null	Het
Spata31d1a	G	A	13: 59,851,702 (GRCm39)	S142L	probably benign	Het
Spopfm2	G	A	3: 94,082,854 (GRCm39)	A319V	possibly damaging	Het
Spopfm2	C	A	3: 94,082,855 (GRCm39)	A319S	possibly damaging	Het
Spopl	C	T	2: 23,427,392 (GRCm39)	R221Q	probably damaging	Het
Strip1	A	G	3: 107,523,135 (GRCm39)	V633A	probably benign	Het
Sun2	A	G	15: 79,612,114 (GRCm39)	S522P	possibly damaging	Het
Tectb	C	G	19: 55,169,431 (GRCm39)		probably benign	Het
Terb2	T	A	2: 122,028,913 (GRCm39)	C157S	probably benign	Het
Themis	T	A	10: 28,739,376 (GRCm39)	N615K	possibly damaging	Het
Tlnrd1	A	T	7: 83,531,488 (GRCm39)	D314E	probably benign	Het
Vmn1r205	T	A	13: 22,776,566 (GRCm39)	T179S	probably benign	Het
Vsig10l	G	A	7: 43,118,185 (GRCm39)	R689H	probably benign	Het
Wt1	G	A	2: 104,993,773 (GRCm39)		probably benign	Het
Zfp423	G	T	8: 88,507,179 (GRCm39)	A1034D	possibly damaging	Het
Zfy2	T	C	Y: 2,107,272 (GRCm39)	E454G	possibly damaging	Het
Zyg11a	G	T	4: 108,053,343 (GRCm39)	Q440K	possibly damaging	Het

Other mutations in Or10al5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01348:Or10al5	APN	17	38,063,177 (GRCm39)	missense	probably benign	0.03
R0100:Or10al5	UTSW	17	38,063,594 (GRCm39)	missense	probably benign	0.12
R0328:Or10al5	UTSW	17	38,063,284 (GRCm39)	missense	possibly damaging	0.57
R1641:Or10al5	UTSW	17	38,062,916 (GRCm39)	missense	possibly damaging	0.90
R3711:Or10al5	UTSW	17	38,063,271 (GRCm39)	nonsense	probably null
R3712:Or10al5	UTSW	17	38,063,271 (GRCm39)	nonsense	probably null
R4658:Or10al5	UTSW	17	38,063,054 (GRCm39)	missense	probably damaging	1.00
R4913:Or10al5	UTSW	17	38,063,315 (GRCm39)	missense	possibly damaging	0.86
R5229:Or10al5	UTSW	17	38,063,192 (GRCm39)	missense	probably benign	0.00
R5611:Or10al5	UTSW	17	38,062,975 (GRCm39)	missense	possibly damaging	0.94
R6159:Or10al5	UTSW	17	38,063,038 (GRCm39)	missense	probably damaging	1.00
R6375:Or10al5	UTSW	17	38,062,990 (GRCm39)	missense	probably benign
R6500:Or10al5	UTSW	17	38,063,577 (GRCm39)	missense	probably damaging	1.00
R6818:Or10al5	UTSW	17	38,063,315 (GRCm39)	missense	possibly damaging	0.86
R7027:Or10al5	UTSW	17	38,063,300 (GRCm39)	missense	probably damaging	1.00
R7498:Or10al5	UTSW	17	38,063,242 (GRCm39)	missense	probably damaging	0.96
R7888:Or10al5	UTSW	17	38,062,888 (GRCm39)	missense	probably damaging	1.00
R8299:Or10al5	UTSW	17	38,062,891 (GRCm39)	nonsense	probably null
R9497:Or10al5	UTSW	17	38,062,942 (GRCm39)	missense	probably damaging	1.00
Z1176:Or10al5	UTSW	17	38,063,658 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAGGCCCGAGAAATCTCTC -3'
(R):5'- GCACTGCAACCAGAATTCTGAC -3'

Sequencing Primer
(F):5'- ATAACTGAGTGCTGTCTACTGGCAG -3'
(R):5'- TGCAACCAGAATTCTGACATAGG -3'

Posted On

2014-10-30