Mutagenetix > Incidental Mutation

Incidental Mutation 'R2359:Vmn1r22'

247063

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r22

Ensembl Gene

ENSMUSG00000115091

Gene Name

vomeronasal 1 receptor 22

Synonyms

V1rc23

MMRRC Submission

040341-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.095) question?

Stock #

R2359 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

57877067-57877975 bp(-) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 57877974 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000154301 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000177435] [ENSMUST00000227342] [ENSMUST00000227650] [ENSMUST00000228076] [ENSMUST00000228257] [ENSMUST00000228322] [ENSMUST00000228905]

AlphaFold

Q8R2D1

Predicted Effect

probably null
Transcript: ENSMUST00000177435
AA Change: M1K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135207
Gene: ENSMUSG00000114982
AA Change: M1K

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	3.9e-57	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000227342

Predicted Effect

probably null
Transcript: ENSMUST00000227650
AA Change: M1K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably null
Transcript: ENSMUST00000228076
AA Change: M1K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably null
Transcript: ENSMUST00000228257
AA Change: M1K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably null
Transcript: ENSMUST00000228322
AA Change: M1K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000228905

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.3%

Validation Efficiency

95% (41/43)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam24	A	G	8: 41,133,984 (GRCm39)	E484G	possibly damaging	Het
Akr1c20	C	T	13: 4,573,276 (GRCm39)	G13D	probably damaging	Het
Alkbh3	T	C	2: 93,838,458 (GRCm39)	T38A	probably benign	Het
Anxa9	A	C	3: 95,210,062 (GRCm39)	L99R	probably damaging	Het
Arfgef2	A	T	2: 166,702,539 (GRCm39)	Y826F	probably damaging	Het
Arid2	G	T	15: 96,259,759 (GRCm39)	L306F	probably damaging	Het
Ccdc141	C	T	2: 77,000,746 (GRCm39)	V29M	probably damaging	Het
Cd55b	A	G	1: 130,345,858 (GRCm39)	S187P	probably damaging	Het
Cep63	A	C	9: 102,471,763 (GRCm39)	L526V	possibly damaging	Het
Chtf8	G	T	8: 107,612,048 (GRCm39)		probably null	Het
Clca4b	T	A	3: 144,631,003 (GRCm39)	S286C	probably damaging	Het
Cpsf1	A	G	15: 76,481,873 (GRCm39)	V1080A	probably benign	Het
Csf2rb2	C	T	15: 78,176,976 (GRCm39)	V165I	probably benign	Het
Cyp2c40	A	T	19: 39,766,398 (GRCm39)	V399E	probably damaging	Het
Dnmt3a	T	C	12: 3,951,599 (GRCm39)	S659P	probably damaging	Het
Efcab3	T	C	11: 104,630,106 (GRCm39)	S967P	possibly damaging	Het
Efr3b	G	A	12: 4,030,136 (GRCm39)		probably benign	Het
Epha8	A	G	4: 136,673,343 (GRCm39)	I147T	probably damaging	Het
Fbxo48	G	A	11: 16,903,602 (GRCm39)	W76*	probably null	Het
Ggt6	A	T	11: 72,328,377 (GRCm39)	L254F	possibly damaging	Het
Gm4204	T	A	1: 135,159,927 (GRCm39)		noncoding transcript	Het
Golph3l	A	G	3: 95,499,275 (GRCm39)		probably null	Het
Gtf2h3	A	G	5: 124,728,939 (GRCm39)	K166R	probably damaging	Het
Hsp90aa1	A	G	12: 110,661,003 (GRCm39)		probably null	Het
Hyi	A	G	4: 118,217,538 (GRCm39)	R79G	probably benign	Het
Igbp1b	G	A	6: 138,634,713 (GRCm39)	P244S	probably damaging	Het
Ipo8	G	A	6: 148,717,975 (GRCm39)		probably benign	Het
Larp4b	C	A	13: 9,208,199 (GRCm39)	T391K	probably damaging	Het
Lrba	T	C	3: 86,256,057 (GRCm39)	V1133A	probably benign	Het
Med13	C	T	11: 86,181,861 (GRCm39)		probably benign	Het
Ncapd2	A	G	6: 125,156,379 (GRCm39)		probably benign	Het
Neurl1b	T	C	17: 26,660,569 (GRCm39)	F299L	probably benign	Het
Nosip	G	A	7: 44,723,450 (GRCm39)	A39T	possibly damaging	Het
Nsd1	A	G	13: 55,361,524 (GRCm39)	D164G	possibly damaging	Het
Ntn1	G	A	11: 68,276,438 (GRCm39)	T170M	probably damaging	Het
Polrmt	A	T	10: 79,572,396 (GRCm39)	L1079Q	probably damaging	Het
Prxl2c	A	T	13: 64,460,465 (GRCm39)	C12S	probably benign	Het
Rab30	A	G	7: 92,485,005 (GRCm39)	D129G	possibly damaging	Het
Rwdd2b	C	T	16: 87,233,809 (GRCm39)	S97N	probably benign	Het
Slitrk3	T	G	3: 72,956,678 (GRCm39)	D698A	possibly damaging	Het
Smarcd2	T	C	11: 106,157,990 (GRCm39)	M93V	probably benign	Het
Tmprss4	A	G	9: 45,097,130 (GRCm39)	V45A	probably benign	Het
Vps13a	A	G	19: 16,630,043 (GRCm39)		probably benign	Het

Other mutations in Vmn1r22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0089:Vmn1r22	UTSW	6	57,877,513 (GRCm39)	missense	probably benign	0.06
R0415:Vmn1r22	UTSW	6	57,877,317 (GRCm39)	missense	probably benign	0.18
R1132:Vmn1r22	UTSW	6	57,877,826 (GRCm39)	missense	probably benign	0.43
R1609:Vmn1r22	UTSW	6	57,877,733 (GRCm39)	nonsense	probably null
R1666:Vmn1r22	UTSW	6	57,877,704 (GRCm39)	missense	probably benign	0.07
R1668:Vmn1r22	UTSW	6	57,877,704 (GRCm39)	missense	probably benign	0.07
R1708:Vmn1r22	UTSW	6	57,877,481 (GRCm39)	missense	possibly damaging	0.46
R1796:Vmn1r22	UTSW	6	57,877,134 (GRCm39)	missense	probably damaging	1.00
R4600:Vmn1r22	UTSW	6	57,877,860 (GRCm39)	missense	probably damaging	1.00
R5302:Vmn1r22	UTSW	6	57,877,960 (GRCm39)	missense	possibly damaging	0.87
R5560:Vmn1r22	UTSW	6	57,877,723 (GRCm39)	missense	probably damaging	1.00
R6026:Vmn1r22	UTSW	6	57,877,390 (GRCm39)	missense	probably benign	0.00
R6066:Vmn1r22	UTSW	6	57,877,864 (GRCm39)	missense	probably benign	0.01
R6343:Vmn1r22	UTSW	6	57,877,563 (GRCm39)	missense	possibly damaging	0.65
R6639:Vmn1r22	UTSW	6	57,877,699 (GRCm39)	missense	probably benign	0.01
R7106:Vmn1r22	UTSW	6	57,877,296 (GRCm39)	missense	probably damaging	1.00
R7683:Vmn1r22	UTSW	6	57,877,404 (GRCm39)	missense	probably damaging	1.00
R8126:Vmn1r22	UTSW	6	57,877,669 (GRCm39)	missense	possibly damaging	0.85
R9216:Vmn1r22	UTSW	6	57,877,257 (GRCm39)	missense	possibly damaging	0.93
R9486:Vmn1r22	UTSW	6	57,877,404 (GRCm39)	missense	probably damaging	1.00
R9707:Vmn1r22	UTSW	6	57,877,890 (GRCm39)	missense	probably benign	0.37
R9722:Vmn1r22	UTSW	6	57,877,631 (GRCm39)	missense	probably benign	0.04
S24628:Vmn1r22	UTSW	6	57,877,317 (GRCm39)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- CCTGCAGTGAGGAACATCAG -3'
(R):5'- GGTGAAATGCAGGGAAAATGTTTTC -3'

Sequencing Primer
(F):5'- CTGCAGTGAGGAACATCAGTATGTG -3'
(R):5'- CTTGGAGGGTATATAACAATGTAGGC -3'

Posted On

2014-10-30