Incidental Mutation 'R2381:Or7g28'
ID 248466
Institutional Source Beutler Lab
Gene Symbol Or7g28
Ensembl Gene ENSMUSG00000058692
Gene Name olfactory receptor family 7 subfamily G member 28
Synonyms GA_x6K02T2PVTD-13098546-13097608, MOR149-3, Olfr846
Accession Numbers
Essential gene? Probably non essential (E-score: 0.139) question?
Stock # R2381 (G1)
Quality Score 225
Status Not validated
Chromosome 9
Chromosomal Location 19271711-19272652 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 19271753 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Aspartic acid at position 299 (E299D)
Ref Sequence ENSEMBL: ENSMUSP00000150672 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000078774] [ENSMUST00000214810]
AlphaFold Q8VET8
Predicted Effect probably benign
Transcript: ENSMUST00000078774
AA Change: E299D

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
SMART Domains Protein: ENSMUSP00000077830
Gene: ENSMUSG00000058692
AA Change: E299D

DomainStartEndE-ValueType
Pfam:7tm_4 31 308 3.2e-54 PFAM
Pfam:7tm_1 41 290 4.7e-23 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000212868
Predicted Effect probably benign
Transcript: ENSMUST00000214810
AA Change: E299D

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 93.4%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 18 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Cd248 G T 19: 5,119,221 (GRCm39) M356I possibly damaging Het
Cul4a T C 8: 13,186,887 (GRCm39) V541A probably benign Het
Dnajb1 T A 8: 84,336,971 (GRCm39) F147Y possibly damaging Het
Dnhd1 A G 7: 105,342,871 (GRCm39) K1405R probably benign Het
Dpys C A 15: 39,705,450 (GRCm39) R221L probably damaging Het
Elapor2 C T 5: 9,430,342 (GRCm39) P84L probably damaging Het
Gm12253 C T 11: 58,326,284 (GRCm39) R100C probably damaging Het
Inppl1 A T 7: 101,478,439 (GRCm39) S592R probably damaging Het
Lct G A 1: 128,231,858 (GRCm39) Q664* probably null Het
Pmepa1 G A 2: 173,069,926 (GRCm39) R210W probably damaging Het
Pnpla7 A C 2: 24,870,770 (GRCm39) K80T probably damaging Het
Ppp4r3c1 T C X: 88,974,116 (GRCm39) I694V probably benign Het
Slc4a1 T C 11: 102,250,128 (GRCm39) D105G probably benign Het
Tpo A G 12: 30,181,826 (GRCm39) I23T possibly damaging Het
Unc5c A G 3: 141,383,916 (GRCm39) E98G probably damaging Het
Usp13 G A 3: 32,935,658 (GRCm39) probably null Het
Vmn1r175 G A 7: 23,508,093 (GRCm39) T178I probably benign Het
Zgrf1 A T 3: 127,349,863 (GRCm39) M15L probably benign Het
Other mutations in Or7g28
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01560:Or7g28 APN 9 19,271,842 (GRCm39) missense probably benign 0.00
IGL01677:Or7g28 APN 9 19,272,441 (GRCm39) missense probably damaging 1.00
PIT4378001:Or7g28 UTSW 9 19,272,471 (GRCm39) missense probably damaging 1.00
R0471:Or7g28 UTSW 9 19,272,177 (GRCm39) nonsense probably null
R4320:Or7g28 UTSW 9 19,272,254 (GRCm39) nonsense probably null
R5093:Or7g28 UTSW 9 19,272,274 (GRCm39) missense probably damaging 1.00
R5109:Or7g28 UTSW 9 19,272,438 (GRCm39) missense probably damaging 1.00
R5130:Or7g28 UTSW 9 19,272,369 (GRCm39) missense possibly damaging 0.81
R6049:Or7g28 UTSW 9 19,272,640 (GRCm39) nonsense probably null
R6937:Or7g28 UTSW 9 19,271,985 (GRCm39) missense probably damaging 0.99
R7024:Or7g28 UTSW 9 19,272,579 (GRCm39) missense possibly damaging 0.64
R8139:Or7g28 UTSW 9 19,272,504 (GRCm39) missense probably damaging 0.99
R9211:Or7g28 UTSW 9 19,271,824 (GRCm39) missense possibly damaging 0.53
R9476:Or7g28 UTSW 9 19,272,383 (GRCm39) missense probably benign 0.23
Predicted Primers PCR Primer
(F):5'- AGCCTAACTGGTAAAAGTCACC -3'
(R):5'- ACTTGTGGGTCTCACCTTTCAG -3'

Sequencing Primer
(F):5'- CACTTCAATGAATCAGCACTTTTTC -3'
(R):5'- GTGGGTCTCACCTTTCAGTTGTC -3'
Posted On 2014-11-11