Mutagenetix > Incidental Mutation

Incidental Mutation 'R0302:Zfr2'

24859

Institutional Source

Beutler Lab

Gene Symbol

Zfr2

Ensembl Gene

ENSMUSG00000034949

Gene Name

zinc finger RNA binding protein 2

Synonyms

2010013I23Rik, 9130206N08Rik

MMRRC Submission

038514-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R0302 (G1)

Quality Score

205

Status

Validated

Chromosome

Chromosomal Location

81068989-81087957 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to T at 81087170 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000113576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000117798] [ENSMUST00000119547]

AlphaFold

E9Q5M4

Predicted Effect

unknown
Transcript: ENSMUST00000117798
AA Change: G843W

SMART Domains

Protein: ENSMUSP00000113913
Gene: ENSMUSG00000034949
AA Change: G843W

Domain	Start	End	E-Value	Type
low complexity region	16	23	N/A	INTRINSIC
low complexity region	44	62	N/A	INTRINSIC
low complexity region	123	163	N/A	INTRINSIC
ZnF_U1	202	236	3.58e-5	SMART
ZnF_C2H2	205	229	7.68e0	SMART
ZnF_U1	249	283	3.78e-4	SMART
ZnF_C2H2	252	276	4.12e0	SMART
ZnF_U1	397	431	3.78e-4	SMART
ZnF_C2H2	400	424	1.99e0	SMART
low complexity region	484	508	N/A	INTRINSIC
DZF	585	837	2.06e-129	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000119547

SMART Domains

Protein: ENSMUSP00000113576
Gene: ENSMUSG00000004933

Domain	Start	End	E-Value	Type
SH3	9	67	1.37e-5	SMART
SH2	78	160	4.87e-31	SMART
TyrKc	193	436	2.88e-129	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132174

Predicted Effect

unknown
Transcript: ENSMUST00000137999
AA Change: R167L

SMART Domains

Protein: ENSMUSP00000120853
Gene: ENSMUSG00000034949
AA Change: R167L

Domain	Start	End	E-Value	Type
Pfam:DZF	2	162	1.3e-32	PFAM
low complexity region	166	178	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148029

Meta Mutation Damage Score

0.1908

Coding Region Coverage

1x: 98.8%
3x: 97.8%
10x: 95.1%
20x: 89.5%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adprs	T	C	4: 126,211,185 (GRCm39)	E244G	probably benign	Het
Aldh1l2	G	A	10: 83,356,229 (GRCm39)	P54S	probably damaging	Het
Ankdd1a	G	A	9: 65,416,924 (GRCm39)		probably benign	Het
Ankra2	T	A	13: 98,408,200 (GRCm39)	S216R	probably damaging	Het
Asah2	A	T	19: 32,030,356 (GRCm39)	N105K	probably benign	Het
Ass1	A	T	2: 31,404,831 (GRCm39)	N371Y	probably damaging	Het
Cacna1s	A	G	1: 136,028,342 (GRCm39)	Y893C	probably benign	Het
Capza2	G	A	6: 17,648,523 (GRCm39)	R15H	probably benign	Het
Cbfa2t2	T	C	2: 154,376,796 (GRCm39)		probably benign	Het
Ccdc96	A	T	5: 36,643,445 (GRCm39)	T484S	possibly damaging	Het
Cckar	GCTTAGCCTCTTCT	GCT	5: 53,857,641 (GRCm39)		probably null	Het
Ccl4	T	A	11: 83,554,280 (GRCm39)		probably benign	Het
Cpt1b	A	G	15: 89,302,073 (GRCm39)	Y702H	probably benign	Het
Cr1l	G	A	1: 194,800,101 (GRCm39)	T153I	probably damaging	Het
Cyth2	C	A	7: 45,460,009 (GRCm39)	E57*	probably null	Het
Daxx	T	A	17: 34,132,594 (GRCm39)	S575T	probably damaging	Het
Depdc5	T	C	5: 33,061,890 (GRCm39)		probably benign	Het
Dnah12	A	G	14: 26,521,956 (GRCm39)	D1923G	probably damaging	Het
Dnah7b	A	G	1: 46,162,937 (GRCm39)	T428A	probably benign	Het
Dnm2	G	T	9: 21,411,639 (GRCm39)	A619S	probably benign	Het
Enpp2	T	C	15: 54,723,457 (GRCm39)	T639A	probably benign	Het
Epsti1	A	T	14: 78,177,366 (GRCm39)	H182L	probably damaging	Het
Exoc3l	C	T	8: 106,020,175 (GRCm39)	R250Q	probably benign	Het
Ggn	G	T	7: 28,870,665 (GRCm39)		probably null	Het
Il1rap	A	G	16: 26,511,544 (GRCm39)	N196S	probably benign	Het
Ints6	T	C	14: 62,946,961 (GRCm39)	T335A	probably damaging	Het
Itga1	G	A	13: 115,148,854 (GRCm39)		probably benign	Het
Kifc3	G	T	8: 95,830,098 (GRCm39)	Q557K	possibly damaging	Het
Krt23	A	G	11: 99,369,027 (GRCm39)	I422T	probably benign	Het
Lcn2	A	G	2: 32,274,901 (GRCm39)		probably benign	Het
Lonp2	A	G	8: 87,364,619 (GRCm39)	T326A	possibly damaging	Het
Lrpprc	T	C	17: 85,047,506 (GRCm39)	I909V	possibly damaging	Het
Lrrc14	G	T	15: 76,598,552 (GRCm39)	R396L	probably benign	Het
Lypd6	T	G	2: 50,055,679 (GRCm39)		probably benign	Het
Man2b1	A	G	8: 85,819,645 (GRCm39)	N610S	probably damaging	Het
Map2	A	T	1: 66,453,987 (GRCm39)	N959I	probably benign	Het
Mctp2	C	T	7: 71,740,012 (GRCm39)	V793I	possibly damaging	Het
Med25	A	C	7: 44,529,982 (GRCm39)		probably benign	Het
Mfsd6	T	C	1: 52,748,616 (GRCm39)	Y83C	probably damaging	Het
Mtbp	A	T	15: 55,488,820 (GRCm39)	M499L	probably damaging	Het
Mtmr10	A	T	7: 63,947,245 (GRCm39)	K53N	probably damaging	Het
Nfat5	T	C	8: 108,085,333 (GRCm39)	I542T	probably damaging	Het
Nr1h3	A	G	2: 91,022,358 (GRCm39)	M90T	probably damaging	Het
Nsmce4a	A	G	7: 130,147,623 (GRCm39)		probably benign	Het
Oprl1	G	A	2: 181,361,021 (GRCm39)	C318Y	probably benign	Het
Or5d40	T	A	2: 88,015,854 (GRCm39)	I211N	possibly damaging	Het
Pbx3	A	T	2: 34,114,572 (GRCm39)	S46T	probably benign	Het
Pign	A	T	1: 105,516,818 (GRCm39)	F575I	possibly damaging	Het
Ptpn13	G	T	5: 103,713,091 (GRCm39)	S1738I	probably benign	Het
Rnf126	G	T	10: 79,595,057 (GRCm39)	P269Q	probably damaging	Het
Ryr3	G	A	2: 112,477,468 (GRCm39)		probably benign	Het
Slc2a7	C	T	4: 150,233,978 (GRCm39)	A31V	probably damaging	Het
Slc6a12	A	G	6: 121,340,218 (GRCm39)	D487G	probably damaging	Het
Son	G	T	16: 91,453,032 (GRCm39)	G593V	probably damaging	Het
Spata31d1a	T	C	13: 59,850,964 (GRCm39)	N388S	probably benign	Het
Spg11	A	T	2: 121,922,668 (GRCm39)	M927K	possibly damaging	Het
Taf13	A	G	3: 108,479,038 (GRCm39)	M1V	probably null	Het
Trim32	G	A	4: 65,531,491 (GRCm39)	R16Q	probably damaging	Het
Trio	A	G	15: 27,902,603 (GRCm39)	F286S	probably damaging	Het
Trpm2	A	C	10: 77,779,824 (GRCm39)		probably benign	Het
Ttc7b	T	C	12: 100,353,438 (GRCm39)	M390V	possibly damaging	Het
Vmn1r184	A	T	7: 25,966,968 (GRCm39)	Q238L	probably damaging	Het
Zfp236	T	C	18: 82,676,213 (GRCm39)	E368G	probably damaging	Het

Other mutations in Zfr2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00672:Zfr2	APN	10	81,077,919 (GRCm39)	missense	probably damaging	0.96
IGL01622:Zfr2	APN	10	81,087,193 (GRCm39)	missense	probably benign
IGL01623:Zfr2	APN	10	81,087,193 (GRCm39)	missense	probably benign
IGL02719:Zfr2	APN	10	81,080,546 (GRCm39)	missense	probably damaging	1.00
IGL03036:Zfr2	APN	10	81,077,985 (GRCm39)	missense	probably benign	0.01
R0837:Zfr2	UTSW	10	81,081,242 (GRCm39)	missense	probably damaging	1.00
R1557:Zfr2	UTSW	10	81,083,225 (GRCm39)	missense	probably benign	0.01
R1714:Zfr2	UTSW	10	81,080,583 (GRCm39)	missense	probably damaging	1.00
R1737:Zfr2	UTSW	10	81,077,919 (GRCm39)	missense	probably damaging	0.96
R1991:Zfr2	UTSW	10	81,078,686 (GRCm39)	missense	possibly damaging	0.86
R2134:Zfr2	UTSW	10	81,078,735 (GRCm39)	missense	probably damaging	1.00
R2148:Zfr2	UTSW	10	81,077,950 (GRCm39)	missense	probably benign	0.13
R2150:Zfr2	UTSW	10	81,077,950 (GRCm39)	missense	probably benign	0.13
R3703:Zfr2	UTSW	10	81,081,913 (GRCm39)	missense	probably benign	0.40
R3704:Zfr2	UTSW	10	81,081,913 (GRCm39)	missense	probably benign	0.40
R3705:Zfr2	UTSW	10	81,081,913 (GRCm39)	missense	probably benign	0.40
R3715:Zfr2	UTSW	10	81,081,913 (GRCm39)	missense	probably benign	0.40
R4301:Zfr2	UTSW	10	81,078,018 (GRCm39)	unclassified	probably benign
R4654:Zfr2	UTSW	10	81,087,083 (GRCm39)	splice site	probably null
R4811:Zfr2	UTSW	10	81,079,547 (GRCm39)	missense	probably benign	0.07
R5290:Zfr2	UTSW	10	81,082,544 (GRCm39)	frame shift	probably null
R5781:Zfr2	UTSW	10	81,079,547 (GRCm39)	missense	probably benign	0.07
R7114:Zfr2	UTSW	10	81,080,559 (GRCm39)	missense	probably damaging	1.00
R8192:Zfr2	UTSW	10	81,078,649 (GRCm39)	missense	possibly damaging	0.83
R8359:Zfr2	UTSW	10	81,078,653 (GRCm39)	missense	possibly damaging	0.57
R8389:Zfr2	UTSW	10	81,081,323 (GRCm39)	missense	probably benign
R8827:Zfr2	UTSW	10	81,078,619 (GRCm39)	missense	probably benign	0.00
R8953:Zfr2	UTSW	10	81,084,271 (GRCm39)	missense	probably damaging	0.99
R9086:Zfr2	UTSW	10	81,076,029 (GRCm39)	missense	probably damaging	0.96
R9189:Zfr2	UTSW	10	81,080,496 (GRCm39)	missense	probably damaging	1.00
R9487:Zfr2	UTSW	10	81,075,969 (GRCm39)	missense	probably benign	0.33
R9592:Zfr2	UTSW	10	81,069,580 (GRCm39)	missense	unknown
R9645:Zfr2	UTSW	10	81,084,252 (GRCm39)	nonsense	probably null
X0063:Zfr2	UTSW	10	81,078,791 (GRCm39)	critical splice donor site	probably null
Z1177:Zfr2	UTSW	10	81,081,918 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGGGTTCCTCTACCCAGGAAGTACAG -3'
(R):5'- GAGGAGGCAATTCAGATCCCATTCAC -3'

Sequencing Primer
(F):5'- AGCTGGTGGTGACATTCCC -3'
(R):5'- CCAAGGAGATCACTGCTATCTGG -3'

Posted On

2013-04-16