Mutagenetix > Incidental Mutation

Incidental Mutation 'R2398:Zmym4'

248590

Institutional Source

Beutler Lab

Gene Symbol

Zmym4

Ensembl Gene

ENSMUSG00000042446

Gene Name

zinc finger, MYM-type 4

Synonyms

Zfp262, 6330503C17Rik

MMRRC Submission

040365-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.513) question?

Stock #

R2398 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

126755732-126861928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 126816929 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 4 (D4E)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106108]

AlphaFold

A2A791

Predicted Effect

probably benign
Transcript: ENSMUST00000106108
AA Change: D256E

PolyPhen 2 Score 0.143 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101714
Gene: ENSMUSG00000042446
AA Change: D256E

Domain	Start	End	E-Value	Type
TRASH	341	377	6.53e-4	SMART
TRASH	389	429	7.22e-6	SMART
TRASH	441	479	1.77e0	SMART
TRASH	486	525	4.95e-4	SMART
TRASH	531	569	1.05e-2	SMART
TRASH	579	615	2.82e1	SMART
low complexity region	640	649	N/A	INTRINSIC
TRASH	687	723	8.49e-3	SMART
TRASH	729	764	1.14e-3	SMART
TRASH	772	810	4.48e-2	SMART
TRASH	816	851	2.06e-1	SMART
low complexity region	974	993	N/A	INTRINSIC
low complexity region	1002	1021	N/A	INTRINSIC
Pfam:DUF3504	1357	1527	1.7e-68	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123597

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129028

Predicted Effect

probably damaging
Transcript: ENSMUST00000135003
AA Change: D4E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000120813
Gene: ENSMUSG00000042446
AA Change: D4E

Domain	Start	End	E-Value	Type
TRASH	90	126	6.53e-4	SMART
TRASH	138	178	7.22e-6	SMART
TRASH	190	228	1.77e0	SMART
TRASH	235	274	3.05e-4	SMART
low complexity region	300	309	N/A	INTRINSIC
TRASH	347	383	8.49e-3	SMART
TRASH	389	424	1.14e-3	SMART
TRASH	432	470	4.48e-2	SMART
TRASH	476	511	2.06e-1	SMART
low complexity region	634	653	N/A	INTRINSIC
low complexity region	662	681	N/A	INTRINSIC
Pfam:DUF3504	1017	1187	1.5e-68	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151026

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154821

Meta Mutation Damage Score

0.0627

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.4%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930568D16Rik	T	C	2: 35,244,872 (GRCm39)	D160G	possibly damaging	Het
Akr1c6	T	C	13: 4,499,035 (GRCm39)	S208P	probably benign	Het
Ap3b2	A	G	7: 81,126,943 (GRCm39)	F269S	probably damaging	Het
Ap3d1	G	A	10: 80,555,006 (GRCm39)	Q440*	probably null	Het
Cog2	T	C	8: 125,256,665 (GRCm39)	I137T	probably benign	Het
Cse1l	A	G	2: 166,770,917 (GRCm39)	Y369C	probably damaging	Het
Dnah9	T	C	11: 65,806,029 (GRCm39)	N3357D	probably damaging	Het
Fam83b	T	C	9: 76,409,500 (GRCm39)	T209A	probably damaging	Het
Gbp2	G	A	3: 142,339,123 (GRCm39)	A392T	probably benign	Het
Grsf1	G	A	5: 88,821,695 (GRCm39)	T123I	probably damaging	Het
Gzmc	T	A	14: 56,470,228 (GRCm39)	I90F	possibly damaging	Het
Hook2	T	A	8: 85,717,928 (GRCm39)	N42K	probably damaging	Het
Hsp90aa1	T	C	12: 110,658,755 (GRCm39)	M629V	possibly damaging	Het
Ifna14	T	C	4: 88,489,863 (GRCm39)	D58G	possibly damaging	Het
Krtap4-8	T	C	11: 99,671,103 (GRCm39)		probably benign	Het
Mocs1	T	C	17: 49,759,862 (GRCm39)	I381T	probably damaging	Het
Nbas	A	G	12: 13,482,946 (GRCm39)	T1408A	probably damaging	Het
Or6c219	A	G	10: 129,781,076 (GRCm39)	F285S	probably benign	Het
Or8j3b	T	A	2: 86,205,183 (GRCm39)	D191V	probably damaging	Het
Orc1	C	T	4: 108,459,166 (GRCm39)	T445I	possibly damaging	Het
Pdik1l	T	C	4: 134,005,710 (GRCm39)	K411E	probably benign	Het
Pias3	A	G	3: 96,611,129 (GRCm39)	I443V	probably benign	Het
Psmd2	T	C	16: 20,478,222 (GRCm39)	S536P	possibly damaging	Het
Rnf10	C	T	5: 115,385,332 (GRCm39)	R554H	probably benign	Het
Smarcc2	C	T	10: 128,305,551 (GRCm39)	T325I	possibly damaging	Het
Smchd1	C	A	17: 71,667,136 (GRCm39)	C1752F	probably damaging	Het
Smchd1	G	A	17: 71,733,431 (GRCm39)		probably benign	Het
Svil	A	G	18: 5,060,613 (GRCm39)		probably null	Het
Tex52	T	C	6: 128,356,540 (GRCm39)	S78P	probably damaging	Het
Tmem63c	T	G	12: 87,103,307 (GRCm39)	V27G	probably damaging	Het
Ttc41	A	G	10: 86,549,250 (GRCm39)	N148S	possibly damaging	Het
Vmn2r67	T	C	7: 84,785,921 (GRCm39)	I695V	probably damaging	Het
Wipf2	T	A	11: 98,789,543 (GRCm39)		probably null	Het
Zc3h18	A	G	8: 123,140,605 (GRCm39)		probably benign	Het
Zfp804a	T	A	2: 82,089,013 (GRCm39)	N947K	possibly damaging	Het
Zfyve26	T	C	12: 79,329,573 (GRCm39)		probably null	Het
Zic2	G	T	14: 122,716,329 (GRCm39)	E422*	probably null	Het

Other mutations in Zmym4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00537:Zmym4	APN	4	126,783,851 (GRCm39)	missense	probably benign
IGL00845:Zmym4	APN	4	126,794,406 (GRCm39)	missense	probably benign	0.28
IGL01122:Zmym4	APN	4	126,758,045 (GRCm39)	missense	probably damaging	1.00
IGL01374:Zmym4	APN	4	126,762,750 (GRCm39)	missense	probably damaging	1.00
IGL01564:Zmym4	APN	4	126,805,073 (GRCm39)	missense	possibly damaging	0.84
IGL02014:Zmym4	APN	4	126,794,462 (GRCm39)	missense	possibly damaging	0.67
IGL02187:Zmym4	APN	4	126,764,066 (GRCm39)	missense	probably damaging	0.97
IGL02887:Zmym4	APN	4	126,842,268 (GRCm39)	missense	probably damaging	0.96
IGL03371:Zmym4	APN	4	126,808,881 (GRCm39)	missense	possibly damaging	0.90
IGL03400:Zmym4	APN	4	126,816,920 (GRCm39)	missense	probably benign	0.12
arriba	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
foreclosed	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
Foreshortened	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
levantese	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
terminated	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
BB004:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
BB014:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R0149:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0361:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0423:Zmym4	UTSW	4	126,776,112 (GRCm39)	splice site	probably benign
R0532:Zmym4	UTSW	4	126,792,194 (GRCm39)	nonsense	probably null
R0745:Zmym4	UTSW	4	126,796,496 (GRCm39)	splice site	probably benign
R1183:Zmym4	UTSW	4	126,819,632 (GRCm39)	missense	probably damaging	0.99
R1401:Zmym4	UTSW	4	126,804,962 (GRCm39)	missense	probably benign	0.00
R1446:Zmym4	UTSW	4	126,776,275 (GRCm39)	missense	probably damaging	1.00
R1491:Zmym4	UTSW	4	126,776,105 (GRCm39)	critical splice acceptor site	probably null
R1566:Zmym4	UTSW	4	126,804,940 (GRCm39)	missense	possibly damaging	0.94
R1962:Zmym4	UTSW	4	126,796,463 (GRCm39)	missense	possibly damaging	0.47
R2930:Zmym4	UTSW	4	126,819,316 (GRCm39)	missense	probably benign	0.00
R3891:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R3892:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R4659:Zmym4	UTSW	4	126,842,221 (GRCm39)	splice site	probably null
R4702:Zmym4	UTSW	4	126,816,958 (GRCm39)	missense	probably benign	0.01
R5160:Zmym4	UTSW	4	126,763,977 (GRCm39)	missense	probably damaging	0.97
R5614:Zmym4	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
R5773:Zmym4	UTSW	4	126,799,163 (GRCm39)	missense	possibly damaging	0.52
R6450:Zmym4	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
R6741:Zmym4	UTSW	4	126,808,878 (GRCm39)	missense	possibly damaging	0.80
R7023:Zmym4	UTSW	4	126,762,593 (GRCm39)	missense	probably damaging	1.00
R7315:Zmym4	UTSW	4	126,776,385 (GRCm39)	missense	probably benign	0.09
R7468:Zmym4	UTSW	4	126,776,029 (GRCm39)	missense	probably benign	0.40
R7546:Zmym4	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
R7609:Zmym4	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
R7764:Zmym4	UTSW	4	126,819,409 (GRCm39)	missense	probably benign	0.06
R7897:Zmym4	UTSW	4	126,783,332 (GRCm39)	missense	possibly damaging	0.76
R7918:Zmym4	UTSW	4	126,804,797 (GRCm39)	critical splice acceptor site	probably null
R7927:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R8129:Zmym4	UTSW	4	126,808,956 (GRCm39)	missense	possibly damaging	0.87
R8240:Zmym4	UTSW	4	126,798,188 (GRCm39)	critical splice donor site	probably null
R8248:Zmym4	UTSW	4	126,799,162 (GRCm39)	missense	possibly damaging	0.56
R8261:Zmym4	UTSW	4	126,798,360 (GRCm39)	missense	probably damaging	1.00
R8313:Zmym4	UTSW	4	126,804,762 (GRCm39)	missense	probably benign	0.19
R8353:Zmym4	UTSW	4	126,800,905 (GRCm39)	missense	possibly damaging	0.46
R8747:Zmym4	UTSW	4	126,787,198 (GRCm39)	missense	probably damaging	1.00
R8787:Zmym4	UTSW	4	126,816,953 (GRCm39)	missense	probably benign	0.41
R8795:Zmym4	UTSW	4	126,799,819 (GRCm39)	missense	probably benign	0.35
R8948:Zmym4	UTSW	4	126,758,060 (GRCm39)	missense	probably damaging	1.00
R9218:Zmym4	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
R9233:Zmym4	UTSW	4	126,776,310 (GRCm39)	missense	probably damaging	0.99
R9286:Zmym4	UTSW	4	126,783,812 (GRCm39)	missense	probably damaging	1.00
R9468:Zmym4	UTSW	4	126,800,993 (GRCm39)	missense	probably benign	0.01
R9542:Zmym4	UTSW	4	126,799,164 (GRCm39)	missense	probably benign	0.00
R9756:Zmym4	UTSW	4	126,771,502 (GRCm39)	missense	probably damaging	1.00
R9776:Zmym4	UTSW	4	126,804,942 (GRCm39)	missense	possibly damaging	0.51
U24488:Zmym4	UTSW	4	126,819,453 (GRCm39)	missense	possibly damaging	0.69
Z1177:Zmym4	UTSW	4	126,801,005 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTGCTACAGCCTTGCAAAAC -3'
(R):5'- GTCCATTCAGAGTTCATTTCCAATG -3'

Sequencing Primer
(F):5'- GCCTTGCAAAACAATTCAAGTTGC -3'
(R):5'- CATTTCCAATGAGCTTTACTTTGG -3'

Posted On

2014-11-11