Incidental Mutation 'R2422:Olfr740'
ID 249393
Institutional Source Beutler Lab
Gene Symbol Olfr740
Ensembl Gene ENSMUSG00000095917
Gene Name olfactory receptor 740
Synonyms MOR106-4, GA_x6K02T2PMLR-6167145-6168080
MMRRC Submission 040384-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.096) question?
Stock # R2422 (G1)
Quality Score 225
Status Validated
Chromosome 14
Chromosomal Location 50445545-50456933 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to C at 50453436 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Leucine to Proline at position 128 (L128P)
Ref Sequence ENSEMBL: ENSMUSP00000148916 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000089838] [ENSMUST00000214792]
AlphaFold E9PV79
Predicted Effect probably damaging
Transcript: ENSMUST00000089838
AA Change: L128P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000087276
Gene: ENSMUSG00000095917
AA Change: L128P

DomainStartEndE-ValueType
Pfam:7tm_4 35 311 2.3e-56 PFAM
Pfam:7tm_1 45 294 1.6e-21 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000214792
AA Change: L128P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
Meta Mutation Damage Score 0.4217 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 94.5%
Validation Efficiency 99% (77/78)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 77 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2010300C02Rik C A 1: 37,613,475 V1084L probably benign Het
4933409G03Rik A T 2: 68,591,520 N47I probably benign Het
Actr5 T C 2: 158,636,081 F457S probably damaging Het
Adamts3 A G 5: 89,683,175 S1007P probably damaging Het
Adck2 C T 6: 39,583,998 A440V possibly damaging Het
Birc6 T A 17: 74,660,614 L301Q probably damaging Het
Ccdc18 A C 5: 108,228,588 E1298D probably damaging Het
Ccdc77 A G 6: 120,339,159 C186R probably benign Het
Cdk12 T G 11: 98,219,074 S640R probably benign Het
Cdv3 T C 9: 103,365,118 probably benign Het
Celf2 G A 2: 6,553,889 T364I probably damaging Het
Cmtr2 C A 8: 110,222,781 S574R probably benign Het
Col14a1 T C 15: 55,449,922 L56P unknown Het
Dctn1 C T 6: 83,199,800 L1241F possibly damaging Het
Depdc5 T C 5: 32,991,035 F1505S probably damaging Het
Dhcr24 G T 4: 106,561,094 probably benign Het
Dnajc21 A G 15: 10,461,935 S127P probably benign Het
Entpd7 A T 19: 43,728,088 Y507F possibly damaging Het
Fbp1 T C 13: 62,871,306 K24E probably benign Het
Galr1 A T 18: 82,405,923 N76K probably damaging Het
Glrb A G 3: 80,860,235 I226T probably damaging Het
Gm17421 T A 12: 113,369,487 noncoding transcript Het
Gm4950 T A 18: 51,865,784 Q33L probably benign Het
Gm5415 C T 1: 32,545,861 A323T possibly damaging Het
Gm6583 A T 5: 112,355,118 V240D probably damaging Het
Gnat2 A T 3: 108,095,539 M88L probably damaging Het
Gpr183 A G 14: 121,954,177 Y311H probably damaging Het
H2-M10.5 A G 17: 36,774,999 I308V probably benign Het
Hipk3 C T 2: 104,471,485 G121R probably benign Het
Hjurp A G 1: 88,266,561 probably benign Het
Homez C A 14: 54,857,574 V226F probably benign Het
Inf2 C A 12: 112,610,824 A1034D unknown Het
Kcnc4 G T 3: 107,445,547 P572T probably benign Het
Kmt2d T C 15: 98,862,266 E1037G unknown Het
Krt78 T C 15: 101,947,264 E704G probably damaging Het
Lama1 A T 17: 67,750,553 M541L probably benign Het
Lmbrd2 C T 15: 9,194,765 T618M possibly damaging Het
Ly6g6e G A 17: 35,078,146 R121Q probably benign Het
Mettl22 T C 16: 8,487,361 F293L probably damaging Het
Mib1 T C 18: 10,751,906 S263P probably damaging Het
Ndufaf1 T C 2: 119,655,737 E298G probably damaging Het
Nek1 T C 8: 61,019,901 V152A probably damaging Het
Nlrp4d T A 7: 10,362,945 D876V probably benign Het
Olfr178 T C 16: 58,889,965 E85G probably benign Het
Olfr235 A T 19: 12,268,919 T230S probably damaging Het
Olfr975 A G 9: 39,950,528 L81P possibly damaging Het
Pcdha11 G T 18: 37,007,272 L651F probably damaging Het
Plekhg1 G A 10: 3,958,048 M988I probably benign Het
Plxnb1 G A 9: 109,108,438 R1169H probably benign Het
Ppp4r3a A G 12: 101,042,653 probably benign Het
Pum2 A T 12: 8,748,931 Q930L possibly damaging Het
Rbp4 T C 19: 38,124,344 E67G probably damaging Het
Rgs9 C A 11: 109,225,777 probably null Het
Sipa1 A T 19: 5,652,112 D923E possibly damaging Het
Smc1a A G X: 152,047,975 probably benign Het
Snx33 C A 9: 56,918,538 M546I probably benign Het
Spag17 T A 3: 100,027,619 W714R probably benign Het
Spata2 C T 2: 167,484,206 R231Q probably damaging Het
Stard9 C T 2: 120,700,284 R2341C probably benign Het
Tas2r116 A T 6: 132,855,594 I53F possibly damaging Het
Tlk1 T C 2: 70,770,005 E110G probably damaging Het
Tmem102 T A 11: 69,804,537 E203V probably benign Het
Top2b T G 14: 16,409,189 I777M probably damaging Het
Ttc23l CT CTTGGATT 15: 10,537,562 probably benign Het
Ttc23l G A 15: 10,537,566 S206L probably benign Het
Tyw5 A G 1: 57,396,748 I82T possibly damaging Het
Uba6 A G 5: 86,132,616 probably null Het
Ugcg C T 4: 59,207,798 P46S probably benign Het
Unc13c T C 9: 73,931,547 Y674C probably damaging Het
Vmn2r105 T A 17: 20,227,835 R242S probably benign Het
Vmn2r12 A G 5: 109,086,532 Y605H probably benign Het
Wdr77 A T 3: 105,960,021 K62* probably null Het
Zfhx4 C A 3: 5,390,405 A1153E probably benign Het
Zfp266 C A 9: 20,499,262 V540L possibly damaging Het
Zfp638 C A 6: 83,966,439 probably benign Het
Zfp644 A G 5: 106,637,244 M479T possibly damaging Het
Zfp951 G A 5: 104,815,277 T141I probably benign Het
Other mutations in Olfr740
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01663:Olfr740 APN 14 50453150 missense probably benign 0.04
IGL02117:Olfr740 APN 14 50453942 missense possibly damaging 0.91
IGL02663:Olfr740 APN 14 50453852 missense probably benign 0.02
IGL02858:Olfr740 APN 14 50453050 utr 5 prime probably benign
IGL02955:Olfr740 APN 14 50453985 missense probably damaging 0.99
IGL03210:Olfr740 APN 14 50453983 missense probably benign 0.10
IGL03249:Olfr740 APN 14 50453211 missense probably damaging 0.98
G1Funyon:Olfr740 UTSW 14 50453564 missense probably benign 0.08
R0946:Olfr740 UTSW 14 50453673 missense probably benign 0.13
R1449:Olfr740 UTSW 14 50453921 missense probably damaging 1.00
R1465:Olfr740 UTSW 14 50453177 missense possibly damaging 0.91
R1465:Olfr740 UTSW 14 50453177 missense possibly damaging 0.91
R1513:Olfr740 UTSW 14 50453681 missense probably benign 0.00
R1908:Olfr740 UTSW 14 50453838 missense probably damaging 0.99
R3406:Olfr740 UTSW 14 50453196 missense probably benign 0.14
R4184:Olfr740 UTSW 14 50453370 missense probably damaging 1.00
R4795:Olfr740 UTSW 14 50453417 missense probably damaging 0.96
R5028:Olfr740 UTSW 14 50453739 missense probably damaging 1.00
R5436:Olfr740 UTSW 14 50453727 missense probably damaging 1.00
R6057:Olfr740 UTSW 14 50453744 nonsense probably null
R6455:Olfr740 UTSW 14 50453585 missense possibly damaging 0.92
R6903:Olfr740 UTSW 14 50453955 missense possibly damaging 0.93
R6998:Olfr740 UTSW 14 50453433 missense probably benign 0.29
R7671:Olfr740 UTSW 14 50453885 missense probably benign 0.04
R8048:Olfr740 UTSW 14 50453916 missense possibly damaging 0.52
R8301:Olfr740 UTSW 14 50453564 missense probably benign 0.08
X0066:Olfr740 UTSW 14 50453658 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- TGTACCTACTGCTGGCCAAC -3'
(R):5'- CCATTAATGGGGCTCTGGAAC -3'

Sequencing Primer
(F):5'- ACTGCTGGCCAACTTCTC -3'
(R):5'- CAATAGAGGGCCTGGGTCACAC -3'
Posted On 2014-11-12