Mutagenetix > Incidental Mutation

Incidental Mutation 'R2436:Vmn2r109'

249606

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

040397-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R2436 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20540517-20564756 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 20554536 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Cysteine at position 186 (G186C)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably damaging
Transcript: ENSMUST00000167093
AA Change: G186C

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: G186C

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg7	A	G	16: 56,761,945 (GRCm38)	S277P	possibly damaging	Het
Arl6ip4	T	C	5: 124,116,599 (GRCm38)	S52P	probably benign	Het
Barx2	T	C	9: 31,913,087 (GRCm38)	H2R	probably damaging	Het
Calhm3	T	C	19: 47,151,965 (GRCm38)	T230A	probably damaging	Het
Card11	C	T	5: 140,882,362 (GRCm38)	V844M	possibly damaging	Het
Dnah6	C	T	6: 73,149,173 (GRCm38)	R1327Q	probably benign	Het
Ehbp1	C	A	11: 22,089,524 (GRCm38)		probably null	Het
Fgg	T	A	3: 83,014,189 (GRCm38)	I393N	possibly damaging	Het
Foxq1	A	T	13: 31,558,533 (GRCm38)		probably benign	Het
Hmgxb3	T	C	18: 61,147,494 (GRCm38)	T646A	probably benign	Het
Homer3	G	A	8: 70,293,056 (GRCm38)	E324K	possibly damaging	Het
Krt80	A	G	15: 101,359,503 (GRCm38)	F183L	probably damaging	Het
Map3k21	A	T	8: 125,941,615 (GRCm38)	K647*	probably null	Het
Mcm3ap	A	G	10: 76,490,057 (GRCm38)	Y1067C	probably damaging	Het
Myh1	G	T	11: 67,213,271 (GRCm38)	Q921H	probably benign	Het
Nme8	A	G	13: 19,677,859 (GRCm38)	F200S	probably damaging	Het
Nploc4	T	C	11: 120,418,317 (GRCm38)	N153S	possibly damaging	Het
Olfr1246	A	G	2: 89,590,773 (GRCm38)	V114A	probably benign	Het
Olfr173	C	T	16: 58,797,244 (GRCm38)	V201I	probably benign	Het
Olfr297	T	C	7: 86,527,383 (GRCm38)	F209L	probably damaging	Het
Olfr319	T	C	11: 58,702,126 (GRCm38)	C142R	probably damaging	Het
Pipox	T	A	11: 77,892,117 (GRCm38)	L86F	probably damaging	Het
Polr3h	G	T	15: 81,917,205 (GRCm38)	L157I	probably benign	Het
Prex2	G	A	1: 11,266,152 (GRCm38)	V1525M	possibly damaging	Het
Rapgef2	A	T	3: 79,088,772 (GRCm38)	D561E	possibly damaging	Het
Sacs	A	T	14: 61,202,905 (GRCm38)	D800V	possibly damaging	Het
Sbsn	A	T	7: 30,752,230 (GRCm38)	L223F	possibly damaging	Het
Slc16a9	TCCCC	TCCCCC	10: 70,256,081 (GRCm38)		probably null	Het
Srrm3	G	T	5: 135,835,176 (GRCm38)	E43*	probably null	Het
Tcaf3	G	A	6: 42,593,729 (GRCm38)	A363V	probably damaging	Het
Tmem138	A	G	19: 10,574,904 (GRCm38)	F78S	probably damaging	Het
Tnfrsf17	G	A	16: 11,319,812 (GRCm38)	D138N	probably damaging	Het
Tubgcp6	C	T	15: 89,102,365 (GRCm38)	V1344I	probably benign	Het
Usp24	T	A	4: 106,409,645 (GRCm38)	L1875*	probably null	Het
Wdr78	T	C	4: 103,066,352 (GRCm38)	I427V	probably benign	Het
Zfp541	A	G	7: 16,076,448 (GRCm38)	N137D	possibly damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20,550,157 (GRCm38)	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20,541,121 (GRCm38)	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20,541,409 (GRCm38)	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20,554,392 (GRCm38)	missense	probably benign
IGL01864:Vmn2r109	APN	17	20,541,134 (GRCm38)	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20,541,080 (GRCm38)	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20,554,341 (GRCm38)	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20,554,160 (GRCm38)	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20,540,888 (GRCm38)	missense	probably benign
IGL02490:Vmn2r109	APN	17	20,540,984 (GRCm38)	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20,540,701 (GRCm38)	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20,554,256 (GRCm38)	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20,553,800 (GRCm38)	missense	probably benign
IGL02745:Vmn2r109	APN	17	20,541,250 (GRCm38)	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20,554,577 (GRCm38)	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20,541,074 (GRCm38)	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20,552,886 (GRCm38)	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20,540,675 (GRCm38)	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20,541,408 (GRCm38)	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20,554,580 (GRCm38)	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20,555,241 (GRCm38)	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20,540,740 (GRCm38)	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20,553,810 (GRCm38)	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20,564,707 (GRCm38)	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20,553,923 (GRCm38)	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20,541,186 (GRCm38)	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20,564,712 (GRCm38)	missense	probably benign	0.00
R3123:Vmn2r109	UTSW	17	20,540,986 (GRCm38)	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20,554,442 (GRCm38)	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20,553,812 (GRCm38)	missense	probably benign
R4428:Vmn2r109	UTSW	17	20,553,024 (GRCm38)	missense	probably benign
R4584:Vmn2r109	UTSW	17	20,554,558 (GRCm38)	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20,541,394 (GRCm38)	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20,541,343 (GRCm38)	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20,553,891 (GRCm38)	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20,541,232 (GRCm38)	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20,550,086 (GRCm38)	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20,555,189 (GRCm38)	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20,554,341 (GRCm38)	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20,540,927 (GRCm38)	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20,540,671 (GRCm38)	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20,540,519 (GRCm38)	makesense	probably null
R5702:Vmn2r109	UTSW	17	20,554,145 (GRCm38)	missense	probably benign	0.42
R5706:Vmn2r109	UTSW	17	20,554,305 (GRCm38)	missense	probably benign	0.16
R5714:Vmn2r109	UTSW	17	20,552,859 (GRCm38)	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20,541,056 (GRCm38)	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20,540,719 (GRCm38)	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20,541,178 (GRCm38)	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20,564,534 (GRCm38)	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20,554,523 (GRCm38)	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20,540,670 (GRCm38)	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20,540,711 (GRCm38)	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20,564,744 (GRCm38)	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20,540,963 (GRCm38)	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20,541,074 (GRCm38)	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20,540,683 (GRCm38)	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20,541,438 (GRCm38)	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20,540,781 (GRCm38)	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20,541,274 (GRCm38)	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20,554,403 (GRCm38)	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20,540,680 (GRCm38)	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20,552,855 (GRCm38)	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20,541,174 (GRCm38)	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20,540,520 (GRCm38)	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20,554,467 (GRCm38)	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20,564,707 (GRCm38)	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20,554,269 (GRCm38)	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20,555,070 (GRCm38)	missense
Z1176:Vmn2r109	UTSW	17	20,552,994 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GGGATCATTTTCACAAAAGCTATGC -3'
(R):5'- CCCTTGAAACTAACAGGTTTGGC -3'

Sequencing Primer
(F):5'- CACAAAAGCTATGCAGATGCCTTTTC -3'
(R):5'- CTAACAGGTTTGGCAGGAATTTC -3'

Posted On

2014-11-12