Incidental Mutation 'R2434:Fmo6'
| ID |
250511 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Fmo6
|
| Ensembl Gene |
ENSMUSG00000095576 |
| Gene Name |
flavin containing monooxygenase 6 |
| Synonyms |
|
| MMRRC Submission |
040395-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.068)
|
| Stock # |
R2434 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
1 |
| Chromosomal Location |
162744120-162765084 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 162744439 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Asparagine to Lysine
at position 484
(N484K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000136378
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000178465]
[ENSMUST00000195576]
|
| AlphaFold |
J3QMN6 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000178465
AA Change: N484K
PolyPhen 2
Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
|
| SMART Domains |
Protein: ENSMUSP00000136378
Gene: ENSMUSG00000095576
AA Change: N484K
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
2 |
532 |
3.2e-273 |
PFAM |
|
Pfam:Pyr_redox_2
|
3 |
226 |
2e-13 |
PFAM |
|
Pfam:Pyr_redox_3
|
6 |
220 |
4.6e-17 |
PFAM |
|
Pfam:K_oxygenase
|
79 |
223 |
1e-7 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000195576
|
| SMART Domains |
Protein: ENSMUSP00000144106
Gene: ENSMUSG00000095576
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
2 |
118 |
5.1e-58 |
PFAM |
|
Pfam:NAD_binding_8
|
7 |
84 |
2.2e-5 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.5%
- 10x: 97.0%
- 20x: 94.0%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 32 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Agbl5 |
A |
G |
5: 31,051,357 (GRCm39) |
Q493R |
probably damaging |
Het |
| Ank3 |
T |
C |
10: 69,837,948 (GRCm39) |
V785A |
probably damaging |
Het |
| Capsl |
A |
T |
15: 9,462,795 (GRCm39) |
H145L |
probably damaging |
Het |
| Carns1 |
A |
C |
19: 4,215,448 (GRCm39) |
C911W |
probably damaging |
Het |
| Celsr2 |
A |
C |
3: 108,311,795 (GRCm39) |
F1351V |
probably damaging |
Het |
| Cpne8 |
T |
C |
15: 90,393,714 (GRCm39) |
I432V |
probably benign |
Het |
| Cpxm1 |
A |
T |
2: 130,236,004 (GRCm39) |
I386N |
probably damaging |
Het |
| E2f5 |
T |
A |
3: 14,644,074 (GRCm39) |
D65E |
probably damaging |
Het |
| Eif3a |
T |
C |
19: 60,752,488 (GRCm39) |
|
probably benign |
Het |
| Fcrl2 |
A |
G |
3: 87,164,005 (GRCm39) |
Y375H |
probably damaging |
Het |
| Fip1l1 |
C |
A |
5: 74,707,485 (GRCm39) |
T196K |
possibly damaging |
Het |
| Foxred1 |
G |
T |
9: 35,116,954 (GRCm39) |
D345E |
probably damaging |
Het |
| Gbe1 |
T |
A |
16: 70,238,100 (GRCm39) |
N295K |
probably damaging |
Het |
| Gipc2 |
T |
A |
3: 151,843,317 (GRCm39) |
I107L |
probably benign |
Het |
| Kcnn1 |
GTCCTCCTCCTCCTCCTCCTC |
GTCCTCCTCCTCCTCCTC |
8: 71,307,810 (GRCm39) |
|
probably benign |
Het |
| Lgr5 |
T |
A |
10: 115,423,311 (GRCm39) |
I30L |
probably benign |
Het |
| Nbea |
A |
T |
3: 55,554,881 (GRCm39) |
V2589E |
possibly damaging |
Het |
| Ncam2 |
A |
T |
16: 81,392,113 (GRCm39) |
N699I |
probably benign |
Het |
| Nlrp1b |
T |
C |
11: 71,047,552 (GRCm39) |
|
probably null |
Het |
| Or11l3 |
A |
G |
11: 58,515,937 (GRCm39) |
Y312H |
possibly damaging |
Het |
| Rnasel |
T |
C |
1: 153,630,396 (GRCm39) |
V304A |
probably damaging |
Het |
| Serinc4 |
C |
T |
2: 121,286,186 (GRCm39) |
R134H |
probably benign |
Het |
| Sim1 |
T |
A |
10: 50,784,054 (GRCm39) |
Y103N |
probably damaging |
Het |
| Slc47a1 |
A |
T |
11: 61,258,548 (GRCm39) |
|
probably null |
Het |
| Slc6a6 |
T |
A |
6: 91,712,193 (GRCm39) |
S241T |
probably benign |
Het |
| Son |
G |
T |
16: 91,451,575 (GRCm39) |
K107N |
probably damaging |
Het |
| St3gal6 |
T |
C |
16: 58,291,015 (GRCm39) |
T329A |
probably damaging |
Het |
| Stab2 |
G |
A |
10: 86,805,183 (GRCm39) |
P265L |
possibly damaging |
Het |
| Tmem199 |
G |
A |
11: 78,400,570 (GRCm39) |
T119I |
probably damaging |
Het |
| Ttc14 |
A |
G |
3: 33,855,227 (GRCm39) |
D125G |
probably benign |
Het |
| Vmn1r205 |
T |
A |
13: 22,776,524 (GRCm39) |
M193L |
probably benign |
Het |
| Vmn2r-ps158 |
A |
G |
7: 42,696,881 (GRCm39) |
Y646C |
probably damaging |
Het |
|
| Other mutations in Fmo6 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01392:Fmo6
|
APN |
1 |
162,757,580 (GRCm39) |
nonsense |
probably null |
|
|
IGL02083:Fmo6
|
APN |
1 |
162,748,033 (GRCm39) |
nonsense |
probably null |
|
|
adventure
|
UTSW |
1 |
162,750,379 (GRCm39) |
missense |
probably benign |
|
|
R0792:Fmo6
|
UTSW |
1 |
162,748,132 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R0940:Fmo6
|
UTSW |
1 |
162,753,795 (GRCm39) |
missense |
probably benign |
0.07 |
|
R1173:Fmo6
|
UTSW |
1 |
162,753,710 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1268:Fmo6
|
UTSW |
1 |
162,748,086 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1538:Fmo6
|
UTSW |
1 |
162,753,675 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1694:Fmo6
|
UTSW |
1 |
162,750,241 (GRCm39) |
missense |
probably benign |
|
|
R1717:Fmo6
|
UTSW |
1 |
162,753,821 (GRCm39) |
nonsense |
probably null |
|
|
R1837:Fmo6
|
UTSW |
1 |
162,750,379 (GRCm39) |
missense |
probably benign |
|
|
R2125:Fmo6
|
UTSW |
1 |
162,757,527 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2894:Fmo6
|
UTSW |
1 |
162,750,293 (GRCm39) |
nonsense |
probably null |
|
|
R4864:Fmo6
|
UTSW |
1 |
162,751,964 (GRCm39) |
missense |
probably benign |
0.34 |
|
R6414:Fmo6
|
UTSW |
1 |
162,748,014 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6576:Fmo6
|
UTSW |
1 |
162,750,264 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6883:Fmo6
|
UTSW |
1 |
162,757,461 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7013:Fmo6
|
UTSW |
1 |
162,745,817 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7014:Fmo6
|
UTSW |
1 |
162,753,877 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7657:Fmo6
|
UTSW |
1 |
162,750,285 (GRCm39) |
missense |
probably benign |
|
|
R8351:Fmo6
|
UTSW |
1 |
162,748,174 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8451:Fmo6
|
UTSW |
1 |
162,748,174 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8911:Fmo6
|
UTSW |
1 |
162,748,114 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
R9217:Fmo6
|
UTSW |
1 |
162,748,046 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9274:Fmo6
|
UTSW |
1 |
162,747,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
X0025:Fmo6
|
UTSW |
1 |
162,750,427 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0025:Fmo6
|
UTSW |
1 |
162,748,065 (GRCm39) |
missense |
probably benign |
0.29 |
|
Z1176:Fmo6
|
UTSW |
1 |
162,753,701 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- ACTTCTAGGCTGGACTCTGG -3'
(R):5'- AACATTGGCAGACTGGGTGC -3'
Sequencing Primer
(F):5'- CTGGCACCCAGCAGACTTTG -3'
(R):5'- TCCTCAGGTTTGGCCAGAG -3'
|
| Posted On |
2014-11-12 |
Incidental Mutation