Mutagenetix > Incidental Mutation

Incidental Mutation 'R2445:Zgpat'

250546

Institutional Source

Beutler Lab

Gene Symbol

Zgpat

Ensembl Gene

ENSMUSG00000027582

Gene Name

zinc finger, CCCH-type with G patch domain

Synonyms

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2445 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

181364928-181383628 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 181366160 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 163 (C163*)

Ref Sequence

ENSEMBL: ENSMUSP00000112067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029105] [ENSMUST00000108807] [ENSMUST00000108808] [ENSMUST00000116366] [ENSMUST00000127988] [ENSMUST00000170190] [ENSMUST00000183499] [ENSMUST00000185118]

AlphaFold

Q8VDM1

Predicted Effect

probably null
Transcript: ENSMUST00000029105
AA Change: C163*

SMART Domains

Protein: ENSMUSP00000029105
Gene: ENSMUSG00000027582
AA Change: C163*

Domain	Start	End	E-Value	Type
low complexity region	53	64	N/A	INTRINSIC
low complexity region	116	128	N/A	INTRINSIC
ZnF_C3H1	174	199	5.99e-4	SMART
SCOP:d1g5va_	218	268	2e-3	SMART
low complexity region	270	287	N/A	INTRINSIC
G_patch	311	357	1.34e-9	SMART
low complexity region	379	392	N/A	INTRINSIC
coiled coil region	431	505	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000048269

Predicted Effect

probably null
Transcript: ENSMUST00000108807
AA Change: C163*

SMART Domains

Protein: ENSMUSP00000104435
Gene: ENSMUSG00000027582
AA Change: C163*

Domain	Start	End	E-Value	Type
low complexity region	53	64	N/A	INTRINSIC
low complexity region	116	128	N/A	INTRINSIC
ZnF_C3H1	174	199	5.99e-4	SMART
SCOP:d1g5va_	218	268	2e-3	SMART
low complexity region	270	287	N/A	INTRINSIC
G_patch	311	357	1.34e-9	SMART
low complexity region	379	392	N/A	INTRINSIC
coiled coil region	431	505	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000108808

SMART Domains

Protein: ENSMUSP00000104436
Gene: ENSMUSG00000038671

Domain	Start	End	E-Value	Type
ARF	1	191	1.71e-31	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000116366
AA Change: C163*

SMART Domains

Protein: ENSMUSP00000112067
Gene: ENSMUSG00000027582
AA Change: C163*

Domain	Start	End	E-Value	Type
low complexity region	53	64	N/A	INTRINSIC
low complexity region	116	128	N/A	INTRINSIC
ZnF_C3H1	174	199	5.99e-4	SMART
SCOP:d1g5va_	218	268	2e-3	SMART
low complexity region	270	287	N/A	INTRINSIC
G_patch	311	357	1.34e-9	SMART
low complexity region	379	392	N/A	INTRINSIC
coiled coil region	431	505	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000127988

SMART Domains

Protein: ENSMUSP00000122066
Gene: ENSMUSG00000038671

Domain	Start	End	E-Value	Type
ARF	1	191	1.71e-31	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130346

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131284

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139368

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145358

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152580

Predicted Effect

probably benign
Transcript: ENSMUST00000170190

SMART Domains

Protein: ENSMUSP00000126387
Gene: ENSMUSG00000038671

Domain	Start	End	E-Value	Type
Pfam:Miro	1	90	1.2e-9	PFAM
Pfam:Arf	1	140	8.5e-38	PFAM
Pfam:Gtr1_RagA	2	110	2.2e-6	PFAM
Pfam:SRPRB	4	118	6e-8	PFAM
Pfam:Ras	4	142	2.7e-12	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000183499

SMART Domains

Protein: ENSMUSP00000138941
Gene: ENSMUSG00000038671

Domain	Start	End	E-Value	Type
Pfam:Arf	4	61	4.8e-8	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184859

Predicted Effect

probably benign
Transcript: ENSMUST00000185118

SMART Domains

Protein: ENSMUSP00000139211
Gene: ENSMUSG00000038671

Domain	Start	End	E-Value	Type
Pfam:Arf	4	120	1.6e-30	PFAM
Pfam:SRPRB	15	116	6.6e-8	PFAM
Pfam:Ras	19	116	2.1e-9	PFAM
Pfam:Miro	19	117	7.3e-12	PFAM
Pfam:MMR_HSR1	19	117	1.2e-7	PFAM
Pfam:Gtr1_RagA	19	119	5.5e-9	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 93.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 19 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,164,546	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,164,547	E685V	possibly damaging	Het
4930562C15Rik	T	A	16: 4,864,397		probably null	Het
Akr1b3	G	T	6: 34,310,934	D163E	probably benign	Het
D130043K22Rik	A	G	13: 24,857,036	D147G	probably benign	Het
F5	T	C	1: 164,190,226	F624S	probably damaging	Het
Gpr182	T	C	10: 127,750,627	T152A	probably benign	Het
Ifna13	A	T	4: 88,643,896	W164R	probably damaging	Het
Lsg1	C	A	16: 30,564,695	R569L	probably benign	Het
Mmp27	T	C	9: 7,581,181	S456P	probably benign	Het
Naip6	A	G	13: 100,300,668	V449A	probably damaging	Het
Obscn	T	C	11: 59,131,646	R758G	possibly damaging	Het
Olfr1130	T	C	2: 87,607,958	I190T	probably damaging	Het
Ptpn23	G	T	9: 110,387,632	P1052Q	possibly damaging	Het
Sacs	A	G	14: 61,205,206	H1567R	probably damaging	Het
Slc30a7	A	T	3: 115,978,653	I280K	probably damaging	Het
Slc6a18	A	T	13: 73,666,752	C15*	probably null	Het
Tnrc18	T	A	5: 142,772,115	I884F	unknown	Het
Trav6d-3	G	A	14: 52,726,828	A83T	probably damaging	Het

Other mutations in Zgpat

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01537:Zgpat	APN	2	181378889	missense	probably benign	0.02
IGL02221:Zgpat	APN	2	181378858	missense	probably benign	0.41
IGL02507:Zgpat	APN	2	181366236	missense	probably damaging	1.00
IGL03124:Zgpat	APN	2	181366180	missense	probably benign	0.05
R0559:Zgpat	UTSW	2	181380192	splice site	probably benign
R1451:Zgpat	UTSW	2	181380191	splice site	probably benign
R1541:Zgpat	UTSW	2	181378865	missense	probably benign	0.01
R1758:Zgpat	UTSW	2	181378840	missense	probably damaging	1.00
R3038:Zgpat	UTSW	2	181366018	unclassified	probably benign
R3700:Zgpat	UTSW	2	181365646	unclassified	probably benign
R4888:Zgpat	UTSW	2	181378858	missense	probably benign	0.41
R5594:Zgpat	UTSW	2	181365627	unclassified	probably benign
R6890:Zgpat	UTSW	2	181378511	missense	probably damaging	0.98
R7023:Zgpat	UTSW	2	181366018	unclassified	probably benign
R7350:Zgpat	UTSW	2	181380435	missense
R7396:Zgpat	UTSW	2	181366089	missense	probably benign	0.00
R7964:Zgpat	UTSW	2	181378481	missense	probably benign	0.03
R9346:Zgpat	UTSW	2	181380051	missense	probably benign
R9377:Zgpat	UTSW	2	181379853	nonsense	probably null
R9561:Zgpat	UTSW	2	181379573	missense	probably benign	0.01
Z1176:Zgpat	UTSW	2	181365729	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTCCAGAAGGCCATTGCG -3'
(R):5'- AAACTAGTTGTGGCGACAGG -3'

Sequencing Primer
(F):5'- CCTGCAATGACTCGGAGAC -3'
(R):5'- GACAGGCCACAGCGGTG -3'

Posted On

2014-11-12