Incidental Mutation 'R2844:Pnoc'
ID 251461
Institutional Source Beutler Lab
Gene Symbol Pnoc
Ensembl Gene ENSMUSG00000045731
Gene Name prepronociceptin
Synonyms OFQ/N, N23K, N/OFQ, proorphanin, Npnc1
MMRRC Submission 040437-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.099) question?
Stock # R2844 (G1)
Quality Score 225
Status Validated
Chromosome 14
Chromosomal Location 65638122-65662921 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 65642284 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Isoleucine at position 160 (F160I)
Ref Sequence ENSEMBL: ENSMUSP00000153589 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000059339] [ENSMUST00000224594]
AlphaFold Q64387
Predicted Effect probably damaging
Transcript: ENSMUST00000059339
AA Change: F160I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000054210
Gene: ENSMUSG00000045731
AA Change: F160I

DomainStartEndE-ValueType
signal peptide 1 19 N/A INTRINSIC
Pfam:Opiods_neuropep 20 66 1.4e-20 PFAM
low complexity region 109 131 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000224594
AA Change: F160I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Meta Mutation Damage Score 0.2378 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 94.8%
Validation Efficiency 98% (42/43)
MGI Phenotype FUNCTION: This gene encodes the precursor for neuropeptides that have been implicated in a wide range of physiological roles such as transmission and sensitivity to pain, learning, memory, anxiety and depression, in the central nervous system. The encoded protein is a precursor that is proteolytically processed to generate multiple biologically active peptides including nociceptin and nocistatin which have opposite functions in pain transmission. Mice lacking the encoded protein display increased anxiety, elevated basal pain threshold and impaired adaptation to repeated stress. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
PHENOTYPE: Mice homozygous for a knock-out allele fail to exhibit prostaglandin E2-induced allodynia. Mice homozygous for a different knock-out allele display increased naloxone-precipitated jumping in response to morphine treatment. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 40 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aadacl2fm1 G A 3: 59,843,830 (GRCm39) V175I probably benign Het
Abcb1a A T 5: 8,736,164 (GRCm39) I186F probably benign Het
Afg3l1 T C 8: 124,221,678 (GRCm39) probably benign Het
Atg4a G A X: 139,893,589 (GRCm39) E106K probably benign Het
Ccdc50 A G 16: 27,225,479 (GRCm39) E64G probably damaging Het
Celsr3 G T 9: 108,706,507 (GRCm39) G997W probably damaging Het
Chd8 C T 14: 52,441,952 (GRCm39) E2138K possibly damaging Het
Col19a1 C T 1: 24,598,762 (GRCm39) G77E unknown Het
Dnaaf11 A G 15: 66,319,525 (GRCm39) probably benign Het
Fhad1 G T 4: 141,632,279 (GRCm39) Q1287K probably benign Het
Fzr1 G T 10: 81,205,252 (GRCm39) T159K probably damaging Het
Gm10608 CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 9: 118,989,784 (GRCm39) probably null Het
Gna13 T C 11: 109,253,951 (GRCm39) I51T probably damaging Het
Gorab A G 1: 163,224,375 (GRCm39) probably null Het
Hydin T C 8: 111,245,746 (GRCm39) V2153A probably benign Het
Ints6 A G 14: 62,942,275 (GRCm39) V486A probably damaging Het
Irx2 A G 13: 72,779,709 (GRCm39) K331R probably damaging Het
Mark2 T C 19: 7,264,227 (GRCm39) E116G probably damaging Het
Med14 A T X: 12,550,235 (GRCm39) H684Q probably benign Het
Or1e25 A T 11: 73,494,209 (GRCm39) T268S probably benign Het
Pde5a T C 3: 122,645,357 (GRCm39) L755P probably damaging Het
Pex14 A T 4: 149,047,968 (GRCm39) I203N probably benign Het
Pi4ka T C 16: 17,168,657 (GRCm39) E691G probably damaging Het
Plekha1 G T 7: 130,510,095 (GRCm39) W280C probably damaging Het
Ppfia3 C A 7: 45,005,852 (GRCm39) R348L probably damaging Het
Ppil6 A T 10: 41,377,689 (GRCm39) probably benign Het
Psmd13 C A 7: 140,477,653 (GRCm39) probably benign Het
Psme4 T C 11: 30,795,173 (GRCm39) probably benign Het
Rfx3 T C 19: 27,784,186 (GRCm39) probably benign Het
Rnase11 A G 14: 51,287,227 (GRCm39) L109S probably damaging Het
Rngtt A G 4: 33,368,678 (GRCm39) T404A probably benign Het
Sbf1 A G 15: 89,187,421 (GRCm39) probably null Het
Sema5b A G 16: 35,480,301 (GRCm39) N656S probably damaging Het
Ssh3 T C 19: 4,315,324 (GRCm39) Y338C probably damaging Het
Tgfbr3l A G 8: 4,299,280 (GRCm39) D49G probably damaging Het
Thbs1 C T 2: 117,948,109 (GRCm39) T423I probably benign Het
Ttc17 A T 2: 94,206,419 (GRCm39) Y243* probably null Het
Zbtb8os A T 4: 129,235,309 (GRCm39) E54D probably damaging Het
Zfp648 A T 1: 154,080,881 (GRCm39) K347* probably null Het
Zfp84 T G 7: 29,474,758 (GRCm39) probably null Het
Other mutations in Pnoc
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0989:Pnoc UTSW 14 65,642,317 (GRCm39) missense probably damaging 1.00
R5852:Pnoc UTSW 14 65,648,671 (GRCm39) missense probably benign 0.03
R7816:Pnoc UTSW 14 65,639,307 (GRCm39) missense possibly damaging 0.83
R8210:Pnoc UTSW 14 65,642,521 (GRCm39) missense probably benign 0.00
R9666:Pnoc UTSW 14 65,639,247 (GRCm39) missense possibly damaging 0.46
Predicted Primers PCR Primer
(F):5'- ATGAAGTCACTTCTTGCCCC -3'
(R):5'- CTGCTCTTTACCAGCCAAAGG -3'

Sequencing Primer
(F):5'- TGCCCCCTGCTAGTTGGTG -3'
(R):5'- CTGAAGAGAATGCCGCGTGTC -3'
Posted On 2014-12-04