Mutagenetix > Incidental Mutation

Incidental Mutation 'R2845:Mrgpra2a'

251517

Institutional Source

Beutler Lab

Gene Symbol

Mrgpra2a

Ensembl Gene

ENSMUSG00000093973

Gene Name

MAS-related GPR, member A2A

Synonyms

MMRRC Submission

040438-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R2845 (G1)

Quality Score

147

Status

Not validated

Chromosome

Chromosomal Location

47076076-47101887 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 47076878 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 127 (M127L)

Ref Sequence

ENSEMBL: ENSMUSP00000124631 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159004]

AlphaFold

A0A0R4J1Z0

Predicted Effect

probably benign
Transcript: ENSMUST00000159004
AA Change: M127L

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000124631
Gene: ENSMUSG00000093973
AA Change: M127L

Domain	Start	End	E-Value	Type
low complexity region	35	48	N/A	INTRINSIC
Pfam:7tm_1	56	222	1.3e-9	PFAM
Pfam:7tm_1	221	288	6.5e-8	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap25	C	T	6: 87,436,949 (GRCm39)	E634K	possibly damaging	Het
Atg4a	G	A	X: 139,893,589 (GRCm39)	E106K	probably benign	Het
Bahd1	G	A	2: 118,753,004 (GRCm39)	R757H	probably damaging	Het
Cep152	A	G	2: 125,429,894 (GRCm39)	I676T	probably damaging	Het
Cherp	C	A	8: 73,220,247 (GRCm39)	A449S	probably damaging	Het
Col19a1	C	T	1: 24,598,762 (GRCm39)	G77E	unknown	Het
Cplane1	G	A	15: 8,245,864 (GRCm39)	R1412H	probably damaging	Het
Csnk1g2	A	G	10: 80,474,438 (GRCm39)	S220G	probably damaging	Het
Efcab7	T	A	4: 99,766,835 (GRCm39)	V20D	probably damaging	Het
Fhad1	G	T	4: 141,632,279 (GRCm39)	Q1287K	probably benign	Het
Frem3	T	C	8: 81,339,849 (GRCm39)	F714S	probably damaging	Het
Gpx6	A	T	13: 21,503,045 (GRCm39)		probably null	Het
Hsd3b1	T	C	3: 98,760,094 (GRCm39)	E299G	probably damaging	Het
Mark2	T	C	19: 7,264,227 (GRCm39)	E116G	probably damaging	Het
Or10al3	T	C	17: 38,011,714 (GRCm39)	I51T	probably damaging	Het
Pign	C	A	1: 105,585,521 (GRCm39)	L9F	possibly damaging	Het
Plekha1	G	T	7: 130,510,095 (GRCm39)	W280C	probably damaging	Het
Plekhh3	T	C	11: 101,061,056 (GRCm39)		probably benign	Het
Pramel22	G	A	4: 143,380,868 (GRCm39)	S385F	probably damaging	Het
Psmd13	C	A	7: 140,477,653 (GRCm39)		probably benign	Het
Ptpru	T	A	4: 131,546,972 (GRCm39)	I168F	probably benign	Het
Sbf1	A	G	15: 89,187,421 (GRCm39)		probably null	Het
Skint10	T	C	4: 112,573,023 (GRCm39)	S258G	probably benign	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Ssh3	T	C	19: 4,315,324 (GRCm39)	Y338C	probably damaging	Het
Tas2r138	T	C	6: 40,589,701 (GRCm39)	S182G	probably benign	Het
Tgfbr3l	A	G	8: 4,299,280 (GRCm39)	D49G	probably damaging	Het
Zbtb8os	A	T	4: 129,235,309 (GRCm39)	E54D	probably damaging	Het
Zfp24	G	T	18: 24,150,885 (GRCm39)	T87K	probably damaging	Het
Zfp407	G	T	18: 84,576,522 (GRCm39)	C1530*	probably null	Het

Other mutations in Mrgpra2a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00160:Mrgpra2a	APN	7	47,076,286 (GRCm39)	missense	probably damaging	0.96
R1487:Mrgpra2a	UTSW	7	47,076,434 (GRCm39)	missense	probably benign	0.29
R1960:Mrgpra2a	UTSW	7	47,076,983 (GRCm39)	missense	probably benign	0.07
R4756:Mrgpra2a	UTSW	7	47,077,114 (GRCm39)	missense	possibly damaging	0.86
R4910:Mrgpra2a	UTSW	7	47,076,292 (GRCm39)	missense	probably benign	0.00
R5654:Mrgpra2a	UTSW	7	47,077,153 (GRCm39)	missense	probably benign	0.01
R6483:Mrgpra2a	UTSW	7	47,076,437 (GRCm39)	missense	probably benign	0.01
R7136:Mrgpra2a	UTSW	7	47,076,934 (GRCm39)	missense	probably benign	0.03
R7139:Mrgpra2a	UTSW	7	47,076,337 (GRCm39)	missense	probably damaging	1.00
R7648:Mrgpra2a	UTSW	7	47,076,411 (GRCm39)	nonsense	probably null
R8932:Mrgpra2a	UTSW	7	47,076,445 (GRCm39)	missense	probably benign	0.01
R9154:Mrgpra2a	UTSW	7	47,076,394 (GRCm39)	missense	probably damaging	1.00
R9245:Mrgpra2a	UTSW	7	47,077,004 (GRCm39)	missense	possibly damaging	0.76
R9273:Mrgpra2a	UTSW	7	47,076,307 (GRCm39)	missense	probably benign	0.05
R9672:Mrgpra2a	UTSW	7	47,076,490 (GRCm39)	missense	probably benign	0.01
R9747:Mrgpra2a	UTSW	7	47,076,458 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGTTAGTTGCCAGACACTCATTG -3'
(R):5'- GAAACGCCATTGTGTTCTGGC -3'

Sequencing Primer
(F):5'- ACACTCATTGTCATTATCATATCTGG -3'
(R):5'- TTCCACTTGCGCAGGAATG -3'

Posted On

2014-12-04