Mutagenetix > Incidental Mutation

Incidental Mutation 'R2508:Wdr24'

251640

Institutional Source

Beutler Lab

Gene Symbol

Wdr24

Ensembl Gene

ENSMUSG00000025737

Gene Name

WD repeat domain 24

Synonyms

MMRRC Submission

040414-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2508 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

26042601-26047704 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 26043273 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 32 (I32F)

Ref Sequence

ENSEMBL: ENSMUSP00000026833 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026832] [ENSMUST00000026833] [ENSMUST00000045692] [ENSMUST00000123582] [ENSMUST00000133595]

AlphaFold

Q8CFJ9

Predicted Effect

probably benign
Transcript: ENSMUST00000026832

SMART Domains

Protein: ENSMUSP00000026832
Gene: ENSMUSG00000025736

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
JmjC	140	271	5.27e-2	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000026833
AA Change: I32F

PolyPhen 2 Score 0.524 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000026833
Gene: ENSMUSG00000025737
AA Change: I32F

Domain	Start	End	E-Value	Type
Blast:WD40	19	53	6e-8	BLAST
WD40	68	103	2.13e1	SMART
WD40	109	149	5.77e-5	SMART
WD40	152	192	4.48e-2	SMART
WD40	196	236	1.48e-11	SMART
WD40	244	282	1.66e0	SMART
WD40	286	327	2.48e0	SMART
low complexity region	605	623	N/A	INTRINSIC
Blast:RING	743	780	2e-7	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000045692

SMART Domains

Protein: ENSMUSP00000048562
Gene: ENSMUSG00000025738

Domain	Start	End	E-Value	Type
low complexity region	49	65	N/A	INTRINSIC
low complexity region	70	89	N/A	INTRINSIC
Blast:FBOX	98	137	2e-14	BLAST
LRR	241	266	1.32e1	SMART
LRR	267	291	1.61e2	SMART
LRR	293	318	1.76e2	SMART
LRR	319	344	3.21e-4	SMART
LRR	345	370	7.67e-2	SMART
LRR	371	396	6.13e-1	SMART
LRR	421	446	3.52e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000123582

SMART Domains

Protein: ENSMUSP00000119840
Gene: ENSMUSG00000025736

Domain	Start	End	E-Value	Type
low complexity region	158	172	N/A	INTRINSIC
low complexity region	194	208	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123692

Predicted Effect

probably benign
Transcript: ENSMUST00000133595

SMART Domains

Protein: ENSMUSP00000122744
Gene: ENSMUSG00000025736

Domain	Start	End	E-Value	Type
transmembrane domain	55	74	N/A	INTRINSIC
JmjC	185	316	5.27e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143296

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180868

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160829

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160349

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144589

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160275

Predicted Effect

probably benign
Transcript: ENSMUST00000152434

SMART Domains

Protein: ENSMUSP00000119378
Gene: ENSMUSG00000025736

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 110 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430548M08Rik	C	T	8: 120,872,132 (GRCm39)	T20I	probably benign	Het
Adamts2	C	T	11: 50,679,516 (GRCm39)	T832I	possibly damaging	Het
Agbl1	G	A	7: 76,239,298 (GRCm39)		probably null	Het
Ankrd61	T	C	5: 143,833,894 (GRCm39)		probably benign	Het
Anpep	T	G	7: 79,488,039 (GRCm39)	Y506S	possibly damaging	Het
Aox1	T	A	1: 58,382,832 (GRCm39)	H1037Q	probably benign	Het
Auh	G	A	13: 53,052,755 (GRCm39)	R47*	probably null	Het
B4galt5	T	A	2: 167,148,558 (GRCm39)	M187L	probably benign	Het
Bub1	A	T	2: 127,643,343 (GRCm39)	D1000E	probably benign	Het
Cacna1f	T	G	X: 7,492,687 (GRCm39)		probably null	Het
Cdh3	T	C	8: 107,279,039 (GRCm39)	L667P	probably damaging	Het
Cenpe	A	T	3: 134,946,834 (GRCm39)	R1116S	possibly damaging	Het
Ces1b	T	A	8: 93,799,969 (GRCm39)	M136L	possibly damaging	Het
Cfap54	T	C	10: 92,833,236 (GRCm39)	E1130G	possibly damaging	Het
Chd9	C	T	8: 91,760,615 (GRCm39)	P2120L	probably benign	Het
Clu	T	C	14: 66,212,452 (GRCm39)	V135A	probably damaging	Het
Cmip	T	A	8: 118,163,432 (GRCm39)	S388T	probably benign	Het
Cnga1	C	T	5: 72,776,404 (GRCm39)	V20I	possibly damaging	Het
Cox4i1	T	A	8: 121,400,029 (GRCm39)	V51E	possibly damaging	Het
Crym	T	C	7: 119,801,050 (GRCm39)	N33S	probably benign	Het
Ctcf	T	A	8: 106,398,016 (GRCm39)	V434E	probably damaging	Het
Daam1	G	A	12: 72,021,997 (GRCm39)	D732N	probably damaging	Het
Dcaf13	A	G	15: 39,008,547 (GRCm39)	Y383C	probably benign	Het
Dock2	T	C	11: 34,262,485 (GRCm39)	T957A	probably benign	Het
Duox1	A	G	2: 122,163,619 (GRCm39)	D817G	probably benign	Het
Eif1ad16	T	C	12: 87,985,258 (GRCm39)	Y95C	probably damaging	Het
Fads3	A	G	19: 10,033,818 (GRCm39)	Y401C	probably damaging	Het
Fam72a	T	C	1: 131,456,592 (GRCm39)		probably null	Het
Fbxw21	C	A	9: 108,974,553 (GRCm39)	K322N	probably benign	Het
Fdxr	G	A	11: 115,162,806 (GRCm39)	T100I	probably damaging	Het
Galnt11	C	G	5: 25,452,610 (GRCm39)	P41A	probably damaging	Het
Glb1l	T	C	1: 75,178,473 (GRCm39)	T322A	probably damaging	Het
Gm6871	T	A	7: 41,197,414 (GRCm39)	T149S	probably benign	Het
Gpr26	C	T	7: 131,568,823 (GRCm39)	T56I	probably damaging	Het
Grik4	C	A	9: 42,533,438 (GRCm39)	G361C	probably damaging	Het
Gsr	T	G	8: 34,170,316 (GRCm39)	D200E	probably benign	Het
Igsf5	T	A	16: 96,165,247 (GRCm39)	D7E	probably benign	Het
Inpp5e	T	A	2: 26,289,355 (GRCm39)	I522F	probably damaging	Het
Insm2	C	A	12: 55,647,096 (GRCm39)	T280K	probably benign	Het
Itih4	T	C	14: 30,617,435 (GRCm39)	V585A	probably damaging	Het
Katnip	T	A	7: 125,394,515 (GRCm39)	V197D	probably benign	Het
Knl1	A	T	2: 118,888,849 (GRCm39)	R17*	probably null	Het
Lag3	G	T	6: 124,888,272 (GRCm39)	L15I	possibly damaging	Het
Lepr	T	A	4: 101,648,093 (GRCm39)	S861T	probably damaging	Het
Mcur1	A	T	13: 43,697,941 (GRCm39)	Y320N	probably damaging	Het
Mgam	A	T	6: 40,736,717 (GRCm39)	D872V	probably damaging	Het
Mlycd	T	C	8: 120,134,446 (GRCm39)		probably null	Het
Mpl	C	A	4: 118,312,954 (GRCm39)	C193F	probably damaging	Het
Mycbp2	C	A	14: 103,368,681 (GRCm39)	A4142S	probably damaging	Het
Myh1	T	A	11: 67,104,424 (GRCm39)	D993E	possibly damaging	Het
N4bp2	T	A	5: 65,947,404 (GRCm39)	D11E	probably benign	Het
Neb	T	C	2: 52,085,533 (GRCm39)	I1521V	probably benign	Het
Notch1	A	G	2: 26,355,485 (GRCm39)	V1744A	possibly damaging	Het
Numa1	T	C	7: 101,644,731 (GRCm39)	S236P	possibly damaging	Het
Or4p20	T	C	2: 88,253,800 (GRCm39)	T190A	possibly damaging	Het
Or6c66	T	A	10: 129,461,784 (GRCm39)	I49L	probably benign	Het
Or8k23	A	G	2: 86,186,708 (GRCm39)	L6P	possibly damaging	Het
Otogl	A	G	10: 107,710,361 (GRCm39)	L576P	probably damaging	Het
P2ry1	C	T	3: 60,910,900 (GRCm39)	T13M	probably damaging	Het
Pak6	A	T	2: 118,525,050 (GRCm39)	R559*	probably null	Het
Parvb	A	T	15: 84,182,171 (GRCm39)	M234L	probably benign	Het
Pcdha11	T	C	18: 37,145,907 (GRCm39)	V666A	possibly damaging	Het
Pde3b	T	A	7: 114,126,092 (GRCm39)	Y775*	probably null	Het
Pgam5	A	G	5: 110,413,869 (GRCm39)	L98P	probably damaging	Het
Phip	T	C	9: 82,797,392 (GRCm39)	H537R	possibly damaging	Het
Pkib	T	G	10: 57,604,205 (GRCm39)	D4E	probably damaging	Het
Plcb1	A	T	2: 135,102,428 (GRCm39)	I202F	probably benign	Het
Pold3	A	G	7: 99,770,590 (GRCm39)	V14A	probably damaging	Het
Prdm1	T	A	10: 44,322,803 (GRCm39)	T249S	probably benign	Het
Prpf39	T	A	12: 65,104,589 (GRCm39)	F551L	probably benign	Het
Prune2	A	G	19: 17,099,986 (GRCm39)	E1830G	probably benign	Het
Ralgapa1	G	A	12: 55,764,986 (GRCm39)	P889S	probably damaging	Het
Rassf2	A	T	2: 131,840,163 (GRCm39)		probably null	Het
Rnf185	A	G	11: 3,368,067 (GRCm39)	Y204H	probably benign	Het
Rpap1	A	G	2: 119,610,535 (GRCm39)		probably null	Het
Rufy3	T	C	5: 88,797,757 (GRCm39)	S645P	probably damaging	Het
Scn11a	T	A	9: 119,594,595 (GRCm39)	Y1266F	probably damaging	Het
Senp7	T	A	16: 55,971,725 (GRCm39)	H287Q	probably benign	Het
Sgsm3	T	C	15: 80,888,073 (GRCm39)		probably null	Het
Slc25a11	A	T	11: 70,536,658 (GRCm39)	V104E	possibly damaging	Het
Slc25a13	G	A	6: 6,117,190 (GRCm39)	T175I	probably benign	Het
Slc26a3	T	A	12: 31,520,902 (GRCm39)	F702Y	probably damaging	Het
Smco2	T	A	6: 146,761,465 (GRCm39)	L184H	probably damaging	Het
Ssc4d	A	T	5: 135,994,461 (GRCm39)	C90S	probably damaging	Het
Sspo	T	C	6: 48,441,298 (GRCm39)	S1835P	probably damaging	Het
Stk35	A	G	2: 129,643,435 (GRCm39)	T140A	probably damaging	Het
Syngr1	C	T	15: 79,995,941 (GRCm39)	T160M	probably damaging	Het
Tent2	A	G	13: 93,320,726 (GRCm39)	L109S	probably damaging	Het
Tgfb1i1	A	G	7: 127,848,085 (GRCm39)		probably null	Het
Thoc1	T	A	18: 9,977,947 (GRCm39)	V296E	probably damaging	Het
Timp2	C	T	11: 118,201,412 (GRCm39)	C75Y	probably damaging	Het
Tlr1	T	C	5: 65,082,639 (GRCm39)	Y646C	probably damaging	Het
Tprn	G	A	2: 25,158,940 (GRCm39)	E655K	possibly damaging	Het
Trbv14	A	C	6: 41,112,424 (GRCm39)	I74L	probably benign	Het
Trbv5	T	A	6: 41,039,555 (GRCm39)	Y53*	probably null	Het
Trpm5	T	C	7: 142,642,656 (GRCm39)	Q97R	possibly damaging	Het
Tut1	A	G	19: 8,932,931 (GRCm39)	D88G	probably damaging	Het
Ucp2	A	T	7: 100,147,620 (GRCm39)	I200F	probably benign	Het
Unc45a	G	A	7: 79,988,623 (GRCm39)	S131L	probably benign	Het
Uty	A	T	Y: 1,158,182 (GRCm39)	H573Q	probably damaging	Het
Vmn1r17	T	A	6: 57,337,855 (GRCm39)	Y121F	possibly damaging	Het
Vmn1r53	T	C	6: 90,200,554 (GRCm39)	I257V	probably benign	Het
Vmn2r90	C	T	17: 17,954,229 (GRCm39)	L798F	probably damaging	Het
Vmn2r94	A	T	17: 18,477,736 (GRCm39)	M225K	probably benign	Het
Zc3h11a	T	A	1: 133,552,521 (GRCm39)	T529S	probably benign	Het
Zc3h4	A	G	7: 16,168,264 (GRCm39)	H791R	unknown	Het
Zfp24	A	G	18: 24,150,927 (GRCm39)	L73P	probably damaging	Het
Zfp616	T	C	11: 73,974,121 (GRCm39)	I130T	probably benign	Het
Zim1	ACAGCAG	ACAGCAGCAG	7: 6,680,429 (GRCm39)		probably benign	Het
Zim1	CAG	CAGAAG	7: 6,680,430 (GRCm39)		probably benign	Het

Other mutations in Wdr24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Wdr24	APN	17	26,045,595 (GRCm39)	missense	probably benign	0.20
IGL01700:Wdr24	APN	17	26,044,802 (GRCm39)	missense	probably damaging	1.00
IGL01763:Wdr24	APN	17	26,045,164 (GRCm39)	missense	probably benign	0.20
IGL02567:Wdr24	APN	17	26,043,322 (GRCm39)	missense	probably damaging	0.99
IGL03100:Wdr24	APN	17	26,044,681 (GRCm39)	missense	possibly damaging	0.47
R0012:Wdr24	UTSW	17	26,046,087 (GRCm39)	missense	probably benign
R0799:Wdr24	UTSW	17	26,045,102 (GRCm39)	missense	probably damaging	1.00
R1015:Wdr24	UTSW	17	26,047,212 (GRCm39)	missense	probably benign	0.12
R1276:Wdr24	UTSW	17	26,046,441 (GRCm39)	missense	probably benign	0.02
R1297:Wdr24	UTSW	17	26,046,322 (GRCm39)	missense	possibly damaging	0.90
R1934:Wdr24	UTSW	17	26,043,240 (GRCm39)	missense	possibly damaging	0.89
R2030:Wdr24	UTSW	17	26,045,017 (GRCm39)	missense	probably benign	0.38
R2069:Wdr24	UTSW	17	26,045,256 (GRCm39)	missense	probably damaging	1.00
R4601:Wdr24	UTSW	17	26,047,181 (GRCm39)	splice site	probably null
R4604:Wdr24	UTSW	17	26,047,479 (GRCm39)	missense	probably damaging	1.00
R4894:Wdr24	UTSW	17	26,045,101 (GRCm39)	missense	probably damaging	1.00
R5068:Wdr24	UTSW	17	26,044,753 (GRCm39)	missense	possibly damaging	0.77
R5088:Wdr24	UTSW	17	26,047,181 (GRCm39)	splice site	probably null
R5104:Wdr24	UTSW	17	26,043,565 (GRCm39)	missense	probably damaging	1.00
R5498:Wdr24	UTSW	17	26,043,535 (GRCm39)	missense	probably damaging	1.00
R5719:Wdr24	UTSW	17	26,047,314 (GRCm39)	critical splice donor site	probably null
R5892:Wdr24	UTSW	17	26,046,960 (GRCm39)	missense	probably benign	0.00
R5975:Wdr24	UTSW	17	26,046,102 (GRCm39)	missense	probably benign	0.37
R6084:Wdr24	UTSW	17	26,043,504 (GRCm39)	missense	probably damaging	0.99
R6106:Wdr24	UTSW	17	26,043,579 (GRCm39)	missense	probably benign
R6114:Wdr24	UTSW	17	26,043,579 (GRCm39)	missense	probably benign
R6116:Wdr24	UTSW	17	26,043,579 (GRCm39)	missense	probably benign
R6165:Wdr24	UTSW	17	26,045,395 (GRCm39)	missense	probably benign	0.18
R6175:Wdr24	UTSW	17	26,045,552 (GRCm39)	missense	probably damaging	1.00
R6331:Wdr24	UTSW	17	26,044,650 (GRCm39)	missense	possibly damaging	0.61
R6548:Wdr24	UTSW	17	26,046,899 (GRCm39)	missense	probably damaging	0.99
R6984:Wdr24	UTSW	17	26,047,209 (GRCm39)	missense	possibly damaging	0.93
R7485:Wdr24	UTSW	17	26,045,101 (GRCm39)	missense	probably damaging	1.00
R7583:Wdr24	UTSW	17	26,044,804 (GRCm39)	missense	probably null	1.00
R7770:Wdr24	UTSW	17	26,046,070 (GRCm39)	missense	probably benign	0.04
R8086:Wdr24	UTSW	17	26,045,101 (GRCm39)	missense	probably damaging	1.00
R8164:Wdr24	UTSW	17	26,044,923 (GRCm39)	splice site	probably null
R9210:Wdr24	UTSW	17	26,043,472 (GRCm39)	missense	probably benign	0.00
R9212:Wdr24	UTSW	17	26,043,472 (GRCm39)	missense	probably benign	0.00
R9567:Wdr24	UTSW	17	26,043,190 (GRCm39)	missense	probably damaging	0.98
R9667:Wdr24	UTSW	17	26,046,301 (GRCm39)	missense	possibly damaging	0.55
X0022:Wdr24	UTSW	17	26,043,246 (GRCm39)	missense	probably damaging	0.99
Z1177:Wdr24	UTSW	17	26,044,661 (GRCm39)	missense	probably benign	0.29

Predicted Primers

PCR Primer
(F):5'- ATAACGACTGGCTGATGGACC -3'
(R):5'- GCAGATTCTCATCCATCTGGTGC -3'

Sequencing Primer
(F):5'- ACTGGCTGATGGACCATCTCAG -3'
(R):5'- GGTGCCAGACCACATCAG -3'

Posted On

2014-12-04