Mutagenetix > Incidental Mutation

Incidental Mutation 'R2848:Or4g7'

251800

Institutional Source

Beutler Lab

Gene Symbol

Or4g7

Ensembl Gene

ENSMUSG00000044039

Gene Name

olfactory receptor family 4 subfamily G member 7

Synonyms

Olfr1288, GA_x6K02T2Q125-72530279-72531217, MOR245-9

MMRRC Submission

040441-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R2848 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

111309131-111310069 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 111309699 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 190 (M190T)

Ref Sequence

ENSEMBL: ENSMUSP00000150331 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000104889] [ENSMUST00000120021] [ENSMUST00000207494] [ENSMUST00000214816]

AlphaFold

Q7TQY0

Predicted Effect

probably benign
Transcript: ENSMUST00000104889
AA Change: M190T

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000100485
Gene: ENSMUSG00000044039
AA Change: M190T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	1.3e-43	PFAM
Pfam:7tm_1	41	287	2.5e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000120021

Predicted Effect

probably benign
Transcript: ENSMUST00000207494

Predicted Effect

probably benign
Transcript: ENSMUST00000214816
AA Change: M190T

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225425

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930451I11Rik	A	T	7: 126,429,894 (GRCm39)	F101Y	possibly damaging	Het
Abca17	G	C	17: 24,508,481 (GRCm39)	T1018R	probably damaging	Het
Adamts14	T	A	10: 61,054,214 (GRCm39)	Q606L	probably damaging	Het
Adgrf5	C	A	17: 43,733,531 (GRCm39)	N118K	possibly damaging	Het
Baz2b	A	T	2: 59,755,010 (GRCm39)	Y1073N	possibly damaging	Het
Celf2	T	C	2: 6,608,936 (GRCm39)	R282G	probably damaging	Het
Cntnap5c	A	T	17: 58,183,387 (GRCm39)	D31V	probably damaging	Het
Cobl	A	G	11: 12,328,342 (GRCm39)	L81P	probably damaging	Het
Cpsf1	A	T	15: 76,487,051 (GRCm39)	L209Q	probably damaging	Het
Crocc	G	A	4: 140,746,067 (GRCm39)	A1684V	probably damaging	Het
Cyp4f37	T	A	17: 32,848,099 (GRCm39)	C206S	probably damaging	Het
Dnah3	A	G	7: 119,567,161 (GRCm39)	V2355A	probably benign	Het
Dnah6	T	C	6: 73,106,314 (GRCm39)	K1756E	probably benign	Het
Fis1	T	C	5: 136,991,971 (GRCm39)	I55T	possibly damaging	Het
Gm2381	G	A	7: 42,469,831 (GRCm39)	P98S	probably damaging	Het
Gpr37	C	T	6: 25,666,945 (GRCm39)		probably benign	Het
Grin2a	A	G	16: 9,579,829 (GRCm39)	F145L	possibly damaging	Het
Htr4	T	C	18: 62,561,197 (GRCm39)	S153P	probably damaging	Het
Igkv9-120	T	A	6: 68,027,128 (GRCm39)		probably benign	Het
Il12rb1	G	A	8: 71,268,446 (GRCm39)	W396*	probably null	Het
Itga8	A	T	2: 12,165,215 (GRCm39)	V798D	probably damaging	Het
Lfng	T	A	5: 140,597,622 (GRCm39)	D149E	probably damaging	Het
Magi2	A	AG	5: 20,807,459 (GRCm39)		probably null	Het
Mgam	C	A	6: 40,629,649 (GRCm39)	A86E	possibly damaging	Het
Myh4	A	G	11: 67,139,459 (GRCm39)	N592S	probably benign	Het
Naa16	A	G	14: 79,573,323 (GRCm39)	C816R	probably damaging	Het
Nek8	A	G	11: 78,058,967 (GRCm39)	S513P	probably damaging	Het
Ociad1	A	G	5: 73,451,694 (GRCm39)		probably null	Het
Or1j4	A	G	2: 36,740,811 (GRCm39)	Y251C	probably damaging	Het
Or2t35	C	T	14: 14,407,398 (GRCm38)	P57S	probably damaging	Het
Osbpl8	T	A	10: 111,105,297 (GRCm39)	S251T	probably benign	Het
Pcdh7	G	A	5: 57,877,618 (GRCm39)	G391E	probably damaging	Het
Pcdhga10	T	A	18: 37,881,253 (GRCm39)	V338E	possibly damaging	Het
Pde4b	C	T	4: 102,458,742 (GRCm39)	A466V	probably damaging	Het
Peg10	C	T	6: 4,756,912 (GRCm39)		probably benign	Het
Poteg	A	T	8: 27,971,704 (GRCm39)	N406I	probably benign	Het
Ppargc1a	A	G	5: 51,631,151 (GRCm39)	F493L	probably benign	Het
Ptpra	C	A	2: 130,386,919 (GRCm39)	H603Q	probably benign	Het
Rnf10	T	C	5: 115,387,171 (GRCm39)	D439G	probably benign	Het
Shfl	A	T	9: 20,784,868 (GRCm39)	H225L	probably damaging	Het
Syt3	G	A	7: 44,042,866 (GRCm39)	V383I	probably benign	Het
Tectb	C	G	19: 55,169,431 (GRCm39)		probably benign	Het
Try5	C	T	6: 41,290,410 (GRCm39)	V25I	probably benign	Het
Ttn	G	T	2: 76,749,551 (GRCm39)	Q3833K	probably benign	Het
Usp53	G	A	3: 122,728,140 (GRCm39)	P814L	probably benign	Het
Utp25	A	T	1: 192,810,759 (GRCm39)	N81K	probably benign	Het
Vmn1r181	G	T	7: 23,683,943 (GRCm39)	S136I	possibly damaging	Het
Vmn2r114	A	T	17: 23,509,948 (GRCm39)	M844K	probably benign	Het
Vwa8	G	T	14: 79,184,582 (GRCm39)	R360L	probably benign	Het
Xlr4b	A	T	X: 72,258,938 (GRCm39)	Q25L	probably null	Het
Zfp532	T	A	18: 65,789,697 (GRCm39)	H1045Q	possibly damaging	Het
Zfp985	G	A	4: 147,667,468 (GRCm39)	W112*	probably null	Het

Other mutations in Or4g7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Or4g7	APN	2	111,309,209 (GRCm39)	missense	probably benign
IGL02021:Or4g7	APN	2	111,309,825 (GRCm39)	missense	probably benign	0.03
IGL02061:Or4g7	APN	2	111,309,614 (GRCm39)	missense	possibly damaging	0.94
R2016:Or4g7	UTSW	2	111,309,532 (GRCm39)	missense	probably benign	0.00
R2017:Or4g7	UTSW	2	111,309,532 (GRCm39)	missense	probably benign	0.00
R2849:Or4g7	UTSW	2	111,309,699 (GRCm39)	missense	probably benign	0.00
R3421:Or4g7	UTSW	2	111,309,297 (GRCm39)	missense	probably benign	0.12
R4223:Or4g7	UTSW	2	111,309,489 (GRCm39)	missense	probably benign	0.00
R4432:Or4g7	UTSW	2	111,309,757 (GRCm39)	nonsense	probably null
R4433:Or4g7	UTSW	2	111,309,757 (GRCm39)	nonsense	probably null
R4476:Or4g7	UTSW	2	111,310,009 (GRCm39)	missense	possibly damaging	0.58
R4631:Or4g7	UTSW	2	111,309,908 (GRCm39)	missense	probably damaging	1.00
R6029:Or4g7	UTSW	2	111,309,310 (GRCm39)	nonsense	probably null
R6036:Or4g7	UTSW	2	111,309,333 (GRCm39)	missense	probably damaging	1.00
R6036:Or4g7	UTSW	2	111,309,333 (GRCm39)	missense	probably damaging	1.00
R6084:Or4g7	UTSW	2	111,309,734 (GRCm39)	missense	probably damaging	1.00
R6329:Or4g7	UTSW	2	111,309,573 (GRCm39)	missense	possibly damaging	0.90
R7307:Or4g7	UTSW	2	111,309,105 (GRCm39)	start gained	probably benign
R7516:Or4g7	UTSW	2	111,309,282 (GRCm39)	missense	probably benign	0.01
R7577:Or4g7	UTSW	2	111,309,477 (GRCm39)	missense	probably damaging	0.98
R8108:Or4g7	UTSW	2	111,309,579 (GRCm39)	missense	possibly damaging	0.90
R8210:Or4g7	UTSW	2	111,309,753 (GRCm39)	missense	possibly damaging	0.89
R8465:Or4g7	UTSW	2	111,309,425 (GRCm39)	missense	probably benign	0.01
R8717:Or4g7	UTSW	2	111,309,992 (GRCm39)	missense	probably damaging	1.00
R8730:Or4g7	UTSW	2	111,309,934 (GRCm39)	missense	probably damaging	1.00
R9360:Or4g7	UTSW	2	111,309,684 (GRCm39)	missense	probably benign	0.10
Z1177:Or4g7	UTSW	2	111,309,552 (GRCm39)	missense	probably damaging	1.00
Z1177:Or4g7	UTSW	2	111,309,159 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CACTACCTGATGATCATGAATCCC -3'
(R):5'- GTGACTTGGTCGGAAATGGC -3'

Sequencing Primer
(F):5'- GCAGTGTCTCAGTTTCTG -3'
(R):5'- CTTGGTCGGAAATGGCCATACATAC -3'

Posted On

2014-12-04