Mutagenetix > Incidental Mutation

Incidental Mutation 'R2853:Vmn2r85'

252414

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r85

Ensembl Gene

ENSMUSG00000092048

Gene Name

vomeronasal 2, receptor 85

Synonyms

EG623734

MMRRC Submission

040446-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R2853 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

130253658-130266615 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 130255035 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 550 (M550L)

Ref Sequence

ENSEMBL: ENSMUSP00000128792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171811]

AlphaFold

G3UW56

Predicted Effect

probably benign
Transcript: ENSMUST00000171811
AA Change: M550L

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000128792
Gene: ENSMUSG00000092048
AA Change: M550L

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	9e-26	PFAM
Pfam:NCD3G	508	562	1.1e-18	PFAM
Pfam:7tm_3	595	831	3.7e-54	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abat	A	C	16: 8,418,832 (GRCm39)	K142T	probably damaging	Het
Als2cl	T	C	9: 110,723,203 (GRCm39)	S636P	probably damaging	Het
Angptl7	T	C	4: 148,584,736 (GRCm39)	E4G	probably benign	Het
Aph1c	T	C	9: 66,741,764 (GRCm39)		probably null	Het
Arhgap23	T	C	11: 97,383,420 (GRCm39)		probably null	Het
Arhgef4	A	C	1: 34,763,129 (GRCm39)	D795A	unknown	Het
Atosb	A	G	4: 43,036,293 (GRCm39)	L146P	probably benign	Het
Ccdc85a	A	G	11: 28,342,942 (GRCm39)		probably benign	Het
Chchd1	T	C	14: 20,754,288 (GRCm39)	S67P	probably benign	Het
Cubn	C	T	2: 13,435,645 (GRCm39)	V1104I	probably benign	Het
Dennd5a	A	T	7: 109,532,878 (GRCm39)	N297K	probably damaging	Het
Dnai4	T	C	4: 102,907,355 (GRCm39)	I644V	possibly damaging	Het
Egflam	A	T	15: 7,249,182 (GRCm39)	W879R	probably damaging	Het
Far1	T	C	7: 113,152,944 (GRCm39)	Y351H	possibly damaging	Het
Gpr62	T	C	9: 106,341,911 (GRCm39)	E339G	probably benign	Het
Hspd1	A	T	1: 55,120,256 (GRCm39)	D315E	probably damaging	Het
Ids	G	T	X: 69,396,776 (GRCm39)	T329K	probably damaging	Het
Itga9	G	A	9: 118,465,604 (GRCm39)	E153K	probably damaging	Het
Krt82	T	C	15: 101,456,870 (GRCm39)	Y170C	probably damaging	Het
Megf10	T	C	18: 57,427,003 (GRCm39)	I1107T	probably damaging	Het
Mre11a	A	G	9: 14,737,843 (GRCm39)	E599G	probably benign	Het
Mtm1	T	G	X: 70,345,389 (GRCm39)	I437S	probably damaging	Het
Ncoa2	C	T	1: 13,257,113 (GRCm39)	V129I	probably damaging	Het
Ncs1	T	C	2: 31,177,329 (GRCm39)	F169L	probably damaging	Het
Ndst2	T	C	14: 20,779,964 (GRCm39)	E92G	probably damaging	Het
Parm1	T	C	5: 91,742,124 (GRCm39)	V164A	probably benign	Het
Pkhd1	T	C	1: 20,128,526 (GRCm39)	Q4059R	probably benign	Het
Scgb1b20	A	C	7: 33,072,949 (GRCm39)	K52N	possibly damaging	Het
Setbp1	T	A	18: 78,967,211 (GRCm39)	Q171L	probably benign	Het
Sik2	A	T	9: 50,809,597 (GRCm39)	L612Q	probably damaging	Het
Srprb	G	A	9: 103,076,038 (GRCm39)	Q800*	probably null	Het
Ss18l1	A	G	2: 179,699,914 (GRCm39)	Y258C	probably damaging	Het
Togaram1	A	G	12: 65,063,386 (GRCm39)	K1567R	probably benign	Het
Ttc6	T	C	12: 57,622,967 (GRCm39)	F122S	probably damaging	Het

Other mutations in Vmn2r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01298:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01361:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL02185:Vmn2r85	APN	10	130,254,561 (GRCm39)	missense	probably benign	0.13
IGL02505:Vmn2r85	APN	10	130,261,449 (GRCm39)	missense	probably damaging	1.00
IGL02607:Vmn2r85	APN	10	130,262,290 (GRCm39)	missense	possibly damaging	0.89
IGL02755:Vmn2r85	APN	10	130,261,381 (GRCm39)	missense	probably damaging	0.98
IGL03188:Vmn2r85	APN	10	130,254,612 (GRCm39)	missense	probably benign	0.16
IGL03366:Vmn2r85	APN	10	130,262,328 (GRCm39)	missense	probably benign	0.00
IGL03397:Vmn2r85	APN	10	130,261,263 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r85	UTSW	10	130,261,572 (GRCm39)	missense	probably benign	0.00
R0066:Vmn2r85	UTSW	10	130,261,770 (GRCm39)	missense	probably damaging	1.00
R0128:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0130:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0503:Vmn2r85	UTSW	10	130,258,609 (GRCm39)	missense	probably damaging	1.00
R0827:Vmn2r85	UTSW	10	130,265,387 (GRCm39)	missense	possibly damaging	0.89
R1432:Vmn2r85	UTSW	10	130,261,155 (GRCm39)	missense	possibly damaging	0.74
R1521:Vmn2r85	UTSW	10	130,261,788 (GRCm39)	missense	probably damaging	0.99
R2029:Vmn2r85	UTSW	10	130,261,443 (GRCm39)	nonsense	probably null
R2034:Vmn2r85	UTSW	10	130,262,242 (GRCm39)	splice site	probably benign
R2852:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R3084:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3085:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3430:Vmn2r85	UTSW	10	130,254,758 (GRCm39)	missense	probably damaging	0.97
R3694:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R3932:Vmn2r85	UTSW	10	130,254,336 (GRCm39)	missense	probably damaging	1.00
R4207:Vmn2r85	UTSW	10	130,254,574 (GRCm39)	missense	probably damaging	1.00
R4628:Vmn2r85	UTSW	10	130,261,235 (GRCm39)	missense	probably benign	0.00
R4814:Vmn2r85	UTSW	10	130,254,567 (GRCm39)	missense	probably benign	0.12
R4948:Vmn2r85	UTSW	10	130,254,990 (GRCm39)	missense	probably damaging	1.00
R4951:Vmn2r85	UTSW	10	130,261,113 (GRCm39)	missense	probably damaging	1.00
R4959:Vmn2r85	UTSW	10	130,257,302 (GRCm39)	missense	probably damaging	1.00
R5336:Vmn2r85	UTSW	10	130,258,574 (GRCm39)	missense	possibly damaging	0.63
R5643:Vmn2r85	UTSW	10	130,262,343 (GRCm39)	missense	probably damaging	1.00
R6061:Vmn2r85	UTSW	10	130,261,531 (GRCm39)	missense	probably benign	0.09
R6115:Vmn2r85	UTSW	10	130,258,672 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r85	UTSW	10	130,261,330 (GRCm39)	missense	possibly damaging	0.88
R6518:Vmn2r85	UTSW	10	130,265,281 (GRCm39)	missense	probably benign	0.00
R6533:Vmn2r85	UTSW	10	130,262,529 (GRCm39)	missense	probably benign	0.00
R6610:Vmn2r85	UTSW	10	130,261,838 (GRCm39)	missense	probably damaging	0.97
R6809:Vmn2r85	UTSW	10	130,261,795 (GRCm39)	missense	probably benign
R6962:Vmn2r85	UTSW	10	130,261,452 (GRCm39)	missense	probably damaging	0.99
R7075:Vmn2r85	UTSW	10	130,258,557 (GRCm39)	missense	probably benign	0.06
R7104:Vmn2r85	UTSW	10	130,262,376 (GRCm39)	missense	probably benign
R7424:Vmn2r85	UTSW	10	130,254,849 (GRCm39)	missense	probably damaging	1.00
R7516:Vmn2r85	UTSW	10	130,254,852 (GRCm39)	missense	probably damaging	1.00
R7537:Vmn2r85	UTSW	10	130,258,735 (GRCm39)	missense	probably benign	0.01
R7768:Vmn2r85	UTSW	10	130,254,562 (GRCm39)	missense	probably damaging	1.00
R7810:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R8078:Vmn2r85	UTSW	10	130,265,364 (GRCm39)	nonsense	probably null
R8115:Vmn2r85	UTSW	10	130,261,820 (GRCm39)	missense	probably benign	0.06
R8262:Vmn2r85	UTSW	10	130,254,738 (GRCm39)	missense	probably damaging	0.98
R8395:Vmn2r85	UTSW	10	130,261,797 (GRCm39)	missense	probably damaging	0.99
R8409:Vmn2r85	UTSW	10	130,261,257 (GRCm39)	missense	probably benign	0.16
R8547:Vmn2r85	UTSW	10	130,261,311 (GRCm39)	missense	probably damaging	1.00
R8875:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R9035:Vmn2r85	UTSW	10	130,261,479 (GRCm39)	missense	probably benign
R9040:Vmn2r85	UTSW	10	130,254,311 (GRCm39)	missense	probably damaging	1.00
R9115:Vmn2r85	UTSW	10	130,254,153 (GRCm39)	missense	probably benign	0.00
R9182:Vmn2r85	UTSW	10	130,265,350 (GRCm39)	missense	probably benign	0.00
R9245:Vmn2r85	UTSW	10	130,261,534 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r85	UTSW	10	130,255,033 (GRCm39)	missense	possibly damaging	0.92
R9405:Vmn2r85	UTSW	10	130,261,215 (GRCm39)	missense	probably damaging	0.99
R9502:Vmn2r85	UTSW	10	130,261,387 (GRCm39)	missense	probably damaging	0.99
R9520:Vmn2r85	UTSW	10	130,254,993 (GRCm39)	missense	probably benign
R9653:Vmn2r85	UTSW	10	130,261,694 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r85	UTSW	10	130,261,713 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r85	UTSW	10	130,254,776 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTTATTGGCCTTCACAATGGG -3'
(R):5'- GATCTGTTCCCTCACAATGCATAG -3'

Sequencing Primer
(F):5'- ATTGGCCTTCACAATGGGAGTATCC -3'
(R):5'- CCCTCACAATGCATAGGTTAATAGAG -3'

Posted On

2014-12-04