Incidental Mutation 'R2864:Crk'
ID 253111
Institutional Source Beutler Lab
Gene Symbol Crk
Ensembl Gene ENSMUSG00000017776
Gene Name v-crk avian sarcoma virus CT10 oncogene homolog
Synonyms Crk-III, Crk-II, Crk-I, Crko, proto-oncogene c-crk
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R2864 (G1)
Quality Score 225
Status Not validated
Chromosome 11
Chromosomal Location 75570085-75597734 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 75594211 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Alanine at position 266 (V266A)
Ref Sequence ENSEMBL: ENSMUSP00000017920 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000017920] [ENSMUST00000093115] [ENSMUST00000108425] [ENSMUST00000108426]
AlphaFold Q64010
PDB Structure CRK SH3 DOMAIN COMPLEXED WITH PEPTOID INHIBITOR [X-RAY DIFFRACTION]
STRUCTURAL BASIS FOR THE SPECIFIC INTERACTION OF LYSINE-CONTAINING PROLINE-RICH PEPTIDES WITH THE N-TERMINAL SH3 DOMAIN OF C-CRK [X-RAY DIFFRACTION]
STRUCTURAL BASIS FOR THE SPECIFIC INTERACTION OF LYSINE-CONTAINING PROLINE-RICH PEPTIDES WITH THE N-TERMINAL SH3 DOMAIN OF C-CRK [X-RAY DIFFRACTION]
Ternary complex of an Crk SH2 domain, Crk-derived phophopeptide, and Abl SH3 domain by NMR spectroscopy [SOLUTION NMR]
Solution structure of N-terminal SH3 domain from oncogene protein c-Crk [SOLUTION NMR]
Solution structure of a circular form of the truncated N-terminal SH3 domain from oncogene protein c-Crk. [SOLUTION NMR]
Solution structure of a circular form of the N-terminal SH3 domain (A134C, E135G, R191G mutant) from oncogene protein c-Crk. [SOLUTION NMR]
Solution structure of a circular form of the N-terminal SH3 domain (E132C, E133G, R191G mutant) from oncogene protein c-Crk [SOLUTION NMR]
Solution structure of the C-terminal SH3 domain of c-CrkII [SOLUTION NMR]
Predicted Effect probably damaging
Transcript: ENSMUST00000017920
AA Change: V266A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000017920
Gene: ENSMUSG00000017776
AA Change: V266A

DomainStartEndE-ValueType
SH2 11 110 4.98e-28 SMART
SH3 135 191 1.82e-19 SMART
SH3 238 295 6.86e-6 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000093115
SMART Domains Protein: ENSMUSP00000090803
Gene: ENSMUSG00000017776

DomainStartEndE-ValueType
SH2 11 110 4.98e-28 SMART
SH3 135 191 1.82e-19 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108425
SMART Domains Protein: ENSMUSP00000104063
Gene: ENSMUSG00000017776

DomainStartEndE-ValueType
SH2 11 110 4.98e-28 SMART
SH3 135 191 1.82e-19 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108426
SMART Domains Protein: ENSMUSP00000104064
Gene: ENSMUSG00000017776

DomainStartEndE-ValueType
SH2 11 72 7.29e-12 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000147718
SMART Domains Protein: ENSMUSP00000116527
Gene: ENSMUSG00000017776

DomainStartEndE-ValueType
SH2 1 71 1.31e0 SMART
SH3 96 152 1.82e-19 SMART
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.5%
Validation Efficiency
MGI Phenotype FUNCTION: This gene is part of a family of adapter proteins that mediate formation of signal transduction complexes in response to extracellular stimuli, such as growth and differentiation factors. Protein-protein interactions occur through the SH2 domain, which binds phosphorylated tyrosine residues, and the SH3 domain, which binds proline-rich peptide motifs. These interactions promote recruitment and activation of effector proteins to regulate cell migration, adhesion, and proliferation. In mouse this protein is essential for embryonic development. Alternatively spliced transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for an isoform specific knockout do not exhibit any obvious abnormalities. Mice homozygous of a null allele of both isoforms exhibit fetal and perinatal lethality associated with abnormal cardiovascular morphology. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 23 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Arhgap30 G T 1: 171,235,774 (GRCm39) G716V probably damaging Het
Dock4 T A 12: 40,780,072 (GRCm39) F624L probably damaging Het
Fhod1 T C 8: 106,059,543 (GRCm39) K714R probably null Het
Flt1 T C 5: 147,531,431 (GRCm39) Q844R possibly damaging Het
Fn1 A G 1: 71,641,578 (GRCm39) V1656A probably damaging Het
Fnip1 T C 11: 54,393,250 (GRCm39) I562T probably damaging Het
Gm7168 A G 17: 14,170,117 (GRCm39) K495E probably benign Het
Igf2r T C 17: 12,905,611 (GRCm39) H2240R probably damaging Het
Itpr3 T C 17: 27,310,525 (GRCm39) V436A probably benign Het
Lrp1b G T 2: 40,765,007 (GRCm39) Q2826K possibly damaging Het
Lrrc37 A G 11: 103,431,744 (GRCm39) F1357S probably benign Het
Luc7l C A 17: 26,485,335 (GRCm39) Q112K probably damaging Het
Misp A G 10: 79,662,872 (GRCm39) K430E probably benign Het
Oprm1 T A 10: 6,744,226 (GRCm39) probably null Het
Or8b43 A G 9: 38,360,684 (GRCm39) N172S possibly damaging Het
Paqr3 C T 5: 97,247,595 (GRCm39) R171H possibly damaging Het
Phc3 C T 3: 30,968,277 (GRCm39) D920N probably damaging Het
Pigk T C 3: 152,428,189 (GRCm39) V72A probably damaging Het
Prickle1 C T 15: 93,407,159 (GRCm39) G112R probably damaging Het
Rrbp1 T C 2: 143,799,557 (GRCm39) E1050G probably damaging Het
Vmn2r93 A T 17: 18,546,323 (GRCm39) I732F probably damaging Het
Xkr6 T C 14: 64,057,205 (GRCm39) L372P unknown Het
Zfp523 T C 17: 28,421,514 (GRCm39) V60A probably benign Het
Other mutations in Crk
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02223:Crk APN 11 75,594,205 (GRCm39) missense probably damaging 0.99
IGL02266:Crk APN 11 75,570,415 (GRCm39) missense probably damaging 1.00
R0282:Crk UTSW 11 75,594,195 (GRCm39) missense probably damaging 1.00
R1956:Crk UTSW 11 75,583,496 (GRCm39) missense possibly damaging 0.83
R5195:Crk UTSW 11 75,570,289 (GRCm39) missense probably damaging 1.00
R8556:Crk UTSW 11 75,583,347 (GRCm39) missense probably benign 0.02
Predicted Primers PCR Primer
(F):5'- ACCACAAGTTAACCAGTCTGG -3'
(R):5'- ACTAGACTGCTTTGACACCTG -3'

Sequencing Primer
(F):5'- CCAGTCTGGTGGCTGGG -3'
(R):5'- CTGCTTTGACACCTGTAAGAAAATTG -3'
Posted On 2014-12-04