Incidental Mutation 'R2471:Zfp101'
| ID |
253128 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp101
|
| Ensembl Gene |
ENSMUSG00000055240 |
| Gene Name |
zinc finger protein 101 |
| Synonyms |
|
| MMRRC Submission |
040403-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.057)
|
| Stock # |
R2471 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
17 |
| Chromosomal Location |
33599148-33613593 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 33599950 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamic Acid to Glycine
at position 602
(E602G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000132508
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000167107]
[ENSMUST00000174417]
[ENSMUST00000174512]
|
| AlphaFold |
Q8BP18 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000167107
AA Change: E602G
PolyPhen 2
Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000132508
Gene: ENSMUSG00000055240
AA Change: E602G
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
10 |
62 |
9.34e-15 |
SMART |
|
ZnF_C2H2
|
219 |
241 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
247 |
269 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
275 |
297 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
302 |
322 |
1.65e2 |
SMART |
|
ZnF_C2H2
|
330 |
352 |
6.75e0 |
SMART |
|
ZnF_C2H2
|
358 |
380 |
2.75e-3 |
SMART |
|
ZnF_C2H2
|
386 |
408 |
4.47e-3 |
SMART |
|
ZnF_C2H2
|
414 |
436 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
442 |
464 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
470 |
492 |
2.2e-2 |
SMART |
|
ZnF_C2H2
|
498 |
520 |
4.47e-3 |
SMART |
|
ZnF_C2H2
|
526 |
548 |
4.72e-2 |
SMART |
|
ZnF_C2H2
|
554 |
576 |
1.28e-3 |
SMART |
|
ZnF_C2H2
|
583 |
605 |
5.06e-2 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000173151
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000174417
|
| SMART Domains |
Protein: ENSMUSP00000134459
Gene: ENSMUSG00000055240
| Domain | Start | End | E-Value | Type |
|---|
|
Blast:KRAB
|
1 |
38 |
5e-16 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000174512
|
| SMART Domains |
Protein: ENSMUSP00000133873
Gene: ENSMUSG00000055240
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
10 |
62 |
9.34e-15 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000183047
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.5%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 35 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930563M21Rik |
A |
T |
9: 55,888,147 (GRCm39) |
M390K |
probably benign |
Het |
| Bicra |
A |
G |
7: 15,706,257 (GRCm39) |
S1395P |
probably benign |
Het |
| Cacnb2 |
G |
A |
2: 14,989,125 (GRCm39) |
D402N |
probably damaging |
Het |
| Csmd1 |
T |
A |
8: 16,261,776 (GRCm39) |
D907V |
probably damaging |
Het |
| Cstl1 |
T |
A |
2: 148,593,041 (GRCm39) |
Y65* |
probably null |
Het |
| Dnajc1 |
T |
C |
2: 18,224,627 (GRCm39) |
I213V |
possibly damaging |
Het |
| Emcn |
C |
T |
3: 137,109,772 (GRCm39) |
T138M |
probably damaging |
Het |
| F3 |
G |
T |
3: 121,518,689 (GRCm39) |
W51L |
probably damaging |
Het |
| Far2 |
A |
G |
6: 148,040,192 (GRCm39) |
Y41C |
probably damaging |
Het |
| Flrt1 |
A |
T |
19: 7,073,856 (GRCm39) |
D230E |
probably damaging |
Het |
| Gimap7 |
G |
A |
6: 48,700,986 (GRCm39) |
V191M |
probably damaging |
Het |
| Gm19402 |
A |
T |
10: 77,526,354 (GRCm39) |
|
probably benign |
Het |
| Ipo5 |
A |
C |
14: 121,159,574 (GRCm39) |
K134T |
probably benign |
Het |
| Kcnf1 |
T |
C |
12: 17,225,531 (GRCm39) |
E230G |
probably damaging |
Het |
| Mad1l1 |
T |
C |
5: 140,247,307 (GRCm39) |
E340G |
probably benign |
Het |
| Map4k5 |
T |
C |
12: 69,903,620 (GRCm39) |
I62V |
probably benign |
Het |
| Marchf7 |
A |
G |
2: 60,067,244 (GRCm39) |
K588R |
possibly damaging |
Het |
| Mctp2 |
T |
A |
7: 71,810,909 (GRCm39) |
K52* |
probably null |
Het |
| Nr1h4 |
A |
T |
10: 89,309,756 (GRCm39) |
F310L |
probably damaging |
Het |
| Ofcc1 |
G |
A |
13: 40,250,501 (GRCm39) |
S605F |
probably damaging |
Het |
| Or2w1b |
A |
T |
13: 21,300,599 (GRCm39) |
T246S |
probably damaging |
Het |
| Or5ac25 |
A |
T |
16: 59,181,944 (GRCm39) |
S212R |
possibly damaging |
Het |
| Or5b97 |
G |
A |
19: 12,878,679 (GRCm39) |
T155I |
probably benign |
Het |
| Pcdh20 |
T |
G |
14: 88,704,672 (GRCm39) |
E876A |
probably benign |
Het |
| Plce1 |
C |
T |
19: 38,768,370 (GRCm39) |
A2215V |
probably damaging |
Het |
| Ptprg |
T |
C |
14: 12,210,327 (GRCm38) |
Y128H |
probably damaging |
Het |
| Sigirr |
A |
G |
7: 140,672,510 (GRCm39) |
L204S |
probably damaging |
Het |
| Slc2a12 |
A |
T |
10: 22,540,706 (GRCm39) |
Y187F |
probably damaging |
Het |
| Slc31a2 |
A |
T |
4: 62,214,312 (GRCm39) |
R86W |
probably null |
Het |
| Smc1b |
T |
C |
15: 84,976,218 (GRCm39) |
K830E |
probably damaging |
Het |
| Ssrp1 |
A |
G |
2: 84,872,642 (GRCm39) |
N427D |
possibly damaging |
Het |
| Taar9 |
A |
T |
10: 23,985,289 (GRCm39) |
F48L |
probably benign |
Het |
| Taok2 |
T |
C |
7: 126,474,272 (GRCm39) |
E319G |
probably damaging |
Het |
| Try10 |
T |
C |
6: 41,333,680 (GRCm39) |
S142P |
probably damaging |
Het |
| Tsc22d1 |
A |
G |
14: 76,655,644 (GRCm39) |
T626A |
probably benign |
Het |
|
| Other mutations in Zfp101 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
R0189:Zfp101
|
UTSW |
17 |
33,601,213 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R0254:Zfp101
|
UTSW |
17 |
33,599,952 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R0448:Zfp101
|
UTSW |
17 |
33,601,295 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R0696:Zfp101
|
UTSW |
17 |
33,600,401 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R0834:Zfp101
|
UTSW |
17 |
33,601,418 (GRCm39) |
missense |
probably benign |
0.33 |
|
R2000:Zfp101
|
UTSW |
17 |
33,600,491 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R2366:Zfp101
|
UTSW |
17 |
33,599,972 (GRCm39) |
missense |
probably benign |
0.06 |
|
R3713:Zfp101
|
UTSW |
17 |
33,600,880 (GRCm39) |
missense |
probably benign |
0.05 |
|
R3857:Zfp101
|
UTSW |
17 |
33,601,405 (GRCm39) |
nonsense |
probably null |
|
|
R4537:Zfp101
|
UTSW |
17 |
33,601,466 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R4712:Zfp101
|
UTSW |
17 |
33,613,457 (GRCm39) |
splice site |
probably null |
|
|
R5049:Zfp101
|
UTSW |
17 |
33,600,872 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R5241:Zfp101
|
UTSW |
17 |
33,601,210 (GRCm39) |
missense |
probably benign |
|
|
R5499:Zfp101
|
UTSW |
17 |
33,601,318 (GRCm39) |
missense |
probably benign |
0.20 |
|
R5587:Zfp101
|
UTSW |
17 |
33,600,295 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R5694:Zfp101
|
UTSW |
17 |
33,599,919 (GRCm39) |
missense |
probably benign |
|
|
R5994:Zfp101
|
UTSW |
17 |
33,599,936 (GRCm39) |
missense |
probably benign |
|
|
R6193:Zfp101
|
UTSW |
17 |
33,600,720 (GRCm39) |
nonsense |
probably null |
|
|
R7331:Zfp101
|
UTSW |
17 |
33,601,559 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R7393:Zfp101
|
UTSW |
17 |
33,605,674 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R7434:Zfp101
|
UTSW |
17 |
33,600,564 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R7922:Zfp101
|
UTSW |
17 |
33,600,511 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R8087:Zfp101
|
UTSW |
17 |
33,599,977 (GRCm39) |
missense |
probably benign |
|
|
R8684:Zfp101
|
UTSW |
17 |
33,600,977 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R9443:Zfp101
|
UTSW |
17 |
33,601,418 (GRCm39) |
missense |
probably benign |
0.33 |
|
R9581:Zfp101
|
UTSW |
17 |
33,605,730 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- GGGCTAAAATTACATTTTGCTGGTC -3'
(R):5'- ACCACTTCCAGTACCCGTAA -3'
Sequencing Primer
(F):5'- ATGACTAGCTAAGCTATCTCGCGTG -3'
(R):5'- TTCCAGTACCCGTAACAGTCATG -3'
|
| Posted On |
2014-12-04 |
Incidental Mutation