Mutagenetix > Incidental Mutations

Incidental Mutation 'R2471:Zfp101'

253128

Institutional Source

Beutler Lab

Gene Symbol

Zfp101

Ensembl Gene

ENSMUSG00000055240

Gene Name

zinc finger protein 101

Synonyms

MMRRC Submission

040403-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R2471 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

33360507-33394641 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 33380976 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 602 (E602G)

Ref Sequence

ENSEMBL: ENSMUSP00000132508 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167107] [ENSMUST00000174417] [ENSMUST00000174512]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167107
AA Change: E602G

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000132508
Gene: ENSMUSG00000055240
AA Change: E602G

Domain	Start	End	E-Value	Type
KRAB	10	62	9.34e-15	SMART
ZnF_C2H2	219	241	2.57e-3	SMART
ZnF_C2H2	247	269	6.32e-3	SMART
ZnF_C2H2	275	297	3.16e-3	SMART
ZnF_C2H2	302	322	1.65e2	SMART
ZnF_C2H2	330	352	6.75e0	SMART
ZnF_C2H2	358	380	2.75e-3	SMART
ZnF_C2H2	386	408	4.47e-3	SMART
ZnF_C2H2	414	436	2.09e-3	SMART
ZnF_C2H2	442	464	4.79e-3	SMART
ZnF_C2H2	470	492	2.2e-2	SMART
ZnF_C2H2	498	520	4.47e-3	SMART
ZnF_C2H2	526	548	4.72e-2	SMART
ZnF_C2H2	554	576	1.28e-3	SMART
ZnF_C2H2	583	605	5.06e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173151

Predicted Effect

probably benign
Transcript: ENSMUST00000174417

SMART Domains

Protein: ENSMUSP00000134459
Gene: ENSMUSG00000055240

Domain	Start	End	E-Value	Type
Blast:KRAB	1	38	5e-16	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000174512

SMART Domains

Protein: ENSMUSP00000133873
Gene: ENSMUSG00000055240

Domain	Start	End	E-Value	Type
KRAB	10	62	9.34e-15	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183047

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563M21Rik	A	T	9: 55,980,863	M390K	probably benign	Het
Bicra	A	G	7: 15,972,332	S1395P	probably benign	Het
Cacnb2	G	A	2: 14,984,314	D402N	probably damaging	Het
Csmd1	T	A	8: 16,211,762	D907V	probably damaging	Het
Cstl1	T	A	2: 148,751,121	Y65*	probably null	Het
Dnajc1	T	C	2: 18,219,816	I213V	possibly damaging	Het
Emcn	C	T	3: 137,404,011	T138M	probably damaging	Het
F3	G	T	3: 121,725,040	W51L	probably damaging	Het
Far2	A	G	6: 148,138,694	Y41C	probably damaging	Het
Flrt1	A	T	19: 7,096,491	D230E	probably damaging	Het
Gimap7	G	A	6: 48,724,052	V191M	probably damaging	Het
Gm19402	A	T	10: 77,690,520		probably benign	Het
Ipo5	A	C	14: 120,922,162	K134T	probably benign	Het
Kcnf1	T	C	12: 17,175,530	E230G	probably damaging	Het
Mad1l1	T	C	5: 140,261,552	E340G	probably benign	Het
Map4k5	T	C	12: 69,856,846	I62V	probably benign	Het
March7	A	G	2: 60,236,900	K588R	possibly damaging	Het
Mctp2	T	A	7: 72,161,161	K52*	probably null	Het
Nr1h4	A	T	10: 89,473,894	F310L	probably damaging	Het
Ofcc1	G	A	13: 40,097,025	S605F	probably damaging	Het
Olfr1369-ps1	A	T	13: 21,116,429	T246S	probably damaging	Het
Olfr1447	G	A	19: 12,901,315	T155I	probably benign	Het
Olfr209	A	T	16: 59,361,581	S212R	possibly damaging	Het
Pcdh20	T	G	14: 88,467,236	E876A	probably benign	Het
Plce1	C	T	19: 38,779,926	A2215V	probably damaging	Het
Ptprg	T	C	14: 12,210,327	Y128H	probably damaging	Het
Sigirr	A	G	7: 141,092,597	L204S	probably damaging	Het
Slc2a12	A	T	10: 22,664,807	Y187F	probably damaging	Het
Slc31a2	A	T	4: 62,296,075	R86W	probably null	Het
Smc1b	T	C	15: 85,092,017	K830E	probably damaging	Het
Ssrp1	A	G	2: 85,042,298	N427D	possibly damaging	Het
Taar9	A	T	10: 24,109,391	F48L	probably benign	Het
Taok2	T	C	7: 126,875,100	E319G	probably damaging	Het
Try10	T	C	6: 41,356,746	S142P	probably damaging	Het
Tsc22d1	A	G	14: 76,418,204	T626A	probably benign	Het

Other mutations in Zfp101

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0189:Zfp101	UTSW	17	33382239	missense	possibly damaging	0.53
R0254:Zfp101	UTSW	17	33380978	missense	possibly damaging	0.71
R0448:Zfp101	UTSW	17	33382321	missense	possibly damaging	0.73
R0696:Zfp101	UTSW	17	33381427	missense	possibly damaging	0.86
R0834:Zfp101	UTSW	17	33382444	missense	probably benign	0.33
R2000:Zfp101	UTSW	17	33381517	missense	possibly damaging	0.92
R2366:Zfp101	UTSW	17	33380998	missense	probably benign	0.06
R3713:Zfp101	UTSW	17	33381906	missense	probably benign	0.05
R3857:Zfp101	UTSW	17	33382431	nonsense	probably null
R4537:Zfp101	UTSW	17	33382492	missense	possibly damaging	0.87
R4712:Zfp101	UTSW	17	33394483	splice site	probably null
R5049:Zfp101	UTSW	17	33381898	missense	possibly damaging	0.71
R5241:Zfp101	UTSW	17	33382236	missense	probably benign
R5499:Zfp101	UTSW	17	33382344	missense	probably benign	0.20
R5587:Zfp101	UTSW	17	33381321	missense	possibly damaging	0.71
R5694:Zfp101	UTSW	17	33380945	missense	probably benign
R5994:Zfp101	UTSW	17	33380962	missense	probably benign
R6193:Zfp101	UTSW	17	33381746	nonsense	probably null
R7331:Zfp101	UTSW	17	33382585	missense	possibly damaging	0.53
R7393:Zfp101	UTSW	17	33386700	missense	possibly damaging	0.71
R7434:Zfp101	UTSW	17	33381590	missense	possibly damaging	0.86
R7922:Zfp101	UTSW	17	33381537	missense	possibly damaging	0.93
R8087:Zfp101	UTSW	17	33381003	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GGGCTAAAATTACATTTTGCTGGTC -3'
(R):5'- ACCACTTCCAGTACCCGTAA -3'

Sequencing Primer
(F):5'- ATGACTAGCTAAGCTATCTCGCGTG -3'
(R):5'- TTCCAGTACCCGTAACAGTCATG -3'

Posted On

2014-12-04