Mutagenetix > Incidental Mutation

Incidental Mutation 'R2474:Vmn2r99'

253308

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

040405-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.164) question?

Stock #

R2474 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19582397-19614852 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19598891 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 192 (M192L)

Ref Sequence

ENSEMBL: ENSMUSP00000156067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably benign
Transcript: ENSMUST00000176107
AA Change: M192L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: M192L

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231989
AA Change: M192L

PolyPhen 2 Score 0.042 (Sensitivity: 0.94; Specificity: 0.83)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 94.9%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl4	A	G	3: 151,248,361 (GRCm39)	T678A	probably benign	Het
Asb7	A	T	7: 66,328,901 (GRCm39)	N46K	probably damaging	Het
Atxn7l2	A	C	3: 108,111,293 (GRCm39)	S414R	probably damaging	Het
Bckdk	C	A	7: 127,504,590 (GRCm39)	R105S	probably damaging	Het
Cxcl13	C	T	5: 96,107,816 (GRCm39)	Q91*	probably null	Het
Dchs1	T	C	7: 105,404,281 (GRCm39)	N2754D	probably benign	Het
Dchs1	A	T	7: 105,422,045 (GRCm39)	V125E	probably damaging	Het
Enpep	G	C	3: 129,077,807 (GRCm39)	S603R	possibly damaging	Het
Greb1	T	C	12: 16,764,954 (GRCm39)	N393S	possibly damaging	Het
Hfm1	A	T	5: 107,020,282 (GRCm39)	V1048D	possibly damaging	Het
Ilvbl	T	C	10: 78,412,558 (GRCm39)	V93A	probably damaging	Het
Itpkb	A	G	1: 180,161,716 (GRCm39)	D614G	probably damaging	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Lgi3	G	A	14: 70,770,689 (GRCm39)		probably null	Het
Mpeg1	G	A	19: 12,439,613 (GRCm39)	C357Y	probably damaging	Het
Nedd1	A	G	10: 92,555,465 (GRCm39)	F7L	probably damaging	Het
Or8g26	A	G	9: 39,095,846 (GRCm39)	D121G	probably damaging	Het
Or8k35	C	A	2: 86,424,957 (GRCm39)	V72F	probably benign	Het
Parp8	A	T	13: 117,029,577 (GRCm39)	C510S	possibly damaging	Het
Phb1	T	C	11: 95,562,248 (GRCm39)	F42L	possibly damaging	Het
Pik3r1	A	T	13: 101,839,284 (GRCm39)	Y189*	probably null	Het
Rps5	T	A	7: 12,660,488 (GRCm39)		probably null	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Senp3	T	A	11: 69,564,923 (GRCm39)	N516Y	probably damaging	Het
Tfap2b	A	T	1: 19,284,599 (GRCm39)	H169L	possibly damaging	Het
Tmem260	A	G	14: 48,733,781 (GRCm39)	D226G	probably null	Het
Ttc6	G	T	12: 57,622,713 (GRCm39)	R37S	probably benign	Het
Vmn2r84	A	T	10: 130,222,392 (GRCm39)	D609E	possibly damaging	Het
Zfp746	T	C	6: 48,041,703 (GRCm39)	D341G	probably damaging	Het
Zfp941	A	T	7: 140,391,384 (GRCm39)	H658Q	probably damaging	Het
Zw10	T	A	9: 48,978,105 (GRCm39)	I351N	probably damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,599,116 (GRCm39)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,614,518 (GRCm39)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,602,885 (GRCm39)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,613,920 (GRCm39)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,600,377 (GRCm39)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,600,494 (GRCm39)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,598,952 (GRCm39)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,598,485 (GRCm39)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,614,547 (GRCm39)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,614,547 (GRCm39)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,614,605 (GRCm39)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,614,835 (GRCm39)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,599,305 (GRCm39)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,582,521 (GRCm39)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,600,322 (GRCm39)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,582,514 (GRCm39)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,598,207 (GRCm39)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,582,415 (GRCm39)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,599,077 (GRCm39)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,598,253 (GRCm39)	missense	probably damaging	1.00
R2519:Vmn2r99	UTSW	17	19,598,970 (GRCm39)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,614,635 (GRCm39)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,599,252 (GRCm39)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,599,252 (GRCm39)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,598,832 (GRCm39)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,599,522 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,613,924 (GRCm39)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,582,397 (GRCm39)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,598,868 (GRCm39)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,599,601 (GRCm39)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,599,531 (GRCm39)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,614,408 (GRCm39)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,598,210 (GRCm39)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,599,242 (GRCm39)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,602,820 (GRCm39)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,602,820 (GRCm39)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,602,867 (GRCm39)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,600,293 (GRCm39)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,600,296 (GRCm39)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,600,457 (GRCm39)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,598,372 (GRCm39)	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19,614,826 (GRCm39)	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19,613,972 (GRCm39)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,599,573 (GRCm39)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,599,407 (GRCm39)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,614,079 (GRCm39)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,600,302 (GRCm39)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,614,020 (GRCm39)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,614,443 (GRCm39)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,613,922 (GRCm39)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,598,388 (GRCm39)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,598,889 (GRCm39)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,582,563 (GRCm39)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,599,563 (GRCm39)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- GTCCTTGAAACTTAGAGGCCTTAC -3'
(R):5'- ATATGAAGTCCATGTGGCGG -3'

Sequencing Primer
(F):5'- ACTTAGAGGCCTTACAAAATTTTCTC -3'
(R):5'- CGGGGGTCATTTTTACAAAAGC -3'

Posted On

2014-12-04