Mutagenetix > Incidental Mutation

Incidental Mutation 'R0314:Vmn2r60'

25373

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Casr-rs3, EG637898, Gprc2a-rs3

MMRRC Submission

038524-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R0314 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41765895-41845200 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 41784985 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably benign
Transcript: ENSMUST00000166447

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Coding Region Coverage

1x: 98.9%
3x: 97.8%
10x: 94.9%
20x: 88.7%

Validation Efficiency

100% (41/41)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arih2	T	C	9: 108,485,878 (GRCm39)	N345D	probably damaging	Het
Ascc3	C	T	10: 50,514,095 (GRCm39)	S298L	possibly damaging	Het
Cacna1e	A	G	1: 154,317,997 (GRCm39)	Y1462H	probably damaging	Het
Car9	T	C	4: 43,509,212 (GRCm39)		probably null	Het
Cenpc1	A	T	5: 86,185,230 (GRCm39)	M427K	probably benign	Het
Chl1	T	A	6: 103,624,262 (GRCm39)	C57S	probably damaging	Het
Cntn3	T	C	6: 102,397,342 (GRCm39)	Y77C	probably damaging	Het
Cobll1	T	C	2: 64,919,865 (GRCm39)	K1187E	possibly damaging	Het
Fkbpl	C	T	17: 34,865,026 (GRCm39)	H265Y	possibly damaging	Het
Fmo1	T	G	1: 162,687,031 (GRCm39)	E32A	probably damaging	Het
Fnip2	G	A	3: 79,388,496 (GRCm39)	T715I	probably damaging	Het
Fzd6	T	A	15: 38,889,128 (GRCm39)	I82K	possibly damaging	Het
Grm5	T	C	7: 87,252,163 (GRCm39)	S138P	probably damaging	Het
Klra5	T	C	6: 129,880,553 (GRCm39)	Y115C	probably damaging	Het
Lgr4	T	C	2: 109,821,438 (GRCm39)		probably benign	Het
Limd1	T	G	9: 123,345,892 (GRCm39)	I557S	probably benign	Het
Mpv17l	A	T	16: 13,758,863 (GRCm39)	I96L	probably benign	Het
Neb	A	T	2: 52,133,343 (GRCm39)	D3398E	probably benign	Het
Nup155	T	C	15: 8,176,736 (GRCm39)	S1005P	probably benign	Het
Or2v2	A	T	11: 49,004,519 (GRCm39)	D11E	possibly damaging	Het
Or52a5b	T	C	7: 103,417,388 (GRCm39)	D72G	probably damaging	Het
Pebp4	G	T	14: 70,297,103 (GRCm39)	S214I	possibly damaging	Het
Pex26	T	C	6: 121,161,443 (GRCm39)		probably null	Het
Rbbp8	A	T	18: 11,848,875 (GRCm39)	Q230L	probably benign	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Robo2	G	A	16: 73,753,525 (GRCm39)	T784M	probably damaging	Het
Slc5a1	T	C	5: 33,303,995 (GRCm39)	I270T	probably benign	Het
Spag4	C	T	2: 155,909,229 (GRCm39)		probably benign	Het
Stt3a	T	C	9: 36,660,841 (GRCm39)		probably benign	Het
Svep1	C	T	4: 58,096,331 (GRCm39)	E1430K	possibly damaging	Het
Timd5	G	A	11: 46,419,364 (GRCm39)	C60Y	probably damaging	Het
Uba6	A	T	5: 86,265,946 (GRCm39)	V956E	probably damaging	Het
Ube2j1	T	A	4: 33,043,991 (GRCm39)		probably benign	Het
Ubr5	A	G	15: 37,997,431 (GRCm39)	S1741P	probably damaging	Het
Vmn2r1	A	G	3: 63,993,980 (GRCm39)	T109A	probably damaging	Het
Vstm2a	A	G	11: 16,318,388 (GRCm39)		probably benign	Het
Zdhhc20	T	A	14: 58,094,076 (GRCm39)	K195N	probably damaging	Het
Zfp683	A	G	4: 133,786,052 (GRCm39)	Y393C	probably benign	Het
Zzz3	T	C	3: 152,133,085 (GRCm39)	S48P	probably benign	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	41,844,578 (GRCm39)	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	41,844,890 (GRCm39)	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	41,845,010 (GRCm39)	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	41,791,720 (GRCm39)	nonsense	probably null
IGL03135:Vmn2r60	APN	7	41,786,018 (GRCm39)	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	41,765,971 (GRCm39)	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	41,844,564 (GRCm39)	missense	possibly damaging	0.54
R0328:Vmn2r60	UTSW	7	41,791,744 (GRCm39)	splice site	probably benign
R0464:Vmn2r60	UTSW	7	41,785,255 (GRCm39)	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	41,844,869 (GRCm39)	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	41,844,365 (GRCm39)	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	41,845,195 (GRCm39)	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	41,786,476 (GRCm39)	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	41,786,137 (GRCm39)	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	41,785,279 (GRCm39)	nonsense	probably null
R1628:Vmn2r60	UTSW	7	41,785,830 (GRCm39)	nonsense	probably null
R1883:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	41,844,931 (GRCm39)	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	41,786,251 (GRCm39)	nonsense	probably null
R2847:Vmn2r60	UTSW	7	41,785,857 (GRCm39)	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	41,790,403 (GRCm39)	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	41,785,220 (GRCm39)	missense	probably benign
R2921:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	41,765,980 (GRCm39)	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	41,785,878 (GRCm39)	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	41,844,667 (GRCm39)	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	41,785,285 (GRCm39)	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	41,786,448 (GRCm39)	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	41,785,154 (GRCm39)	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	41,845,049 (GRCm39)	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	41,765,932 (GRCm39)	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	41,844,847 (GRCm39)	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	41,786,400 (GRCm39)	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	41,785,045 (GRCm39)	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	41,765,895 (GRCm39)	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	41,844,310 (GRCm39)	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	41,791,716 (GRCm39)	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	41,791,666 (GRCm39)	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	41,786,487 (GRCm39)	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	41,844,536 (GRCm39)	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	41,786,166 (GRCm39)	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	41,785,826 (GRCm39)	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	41,844,631 (GRCm39)	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7527:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7528:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7764:Vmn2r60	UTSW	7	41,844,535 (GRCm39)	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	41,844,511 (GRCm39)	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	41,790,521 (GRCm39)	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	41,791,690 (GRCm39)	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	41,790,494 (GRCm39)	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	41,844,954 (GRCm39)	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	41,844,850 (GRCm39)	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	41,786,169 (GRCm39)	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	41,791,654 (GRCm39)	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	41,790,518 (GRCm39)	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	41,785,778 (GRCm39)	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	41,791,723 (GRCm39)	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	41,785,955 (GRCm39)	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	41,844,332 (GRCm39)	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	41,785,172 (GRCm39)	missense	probably benign
RF024:Vmn2r60	UTSW	7	41,790,363 (GRCm39)	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	41,790,538 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGTAGAGACTGAAAGGCCAAGACCC -3'
(R):5'- ACTTATCCCTGTGCGCCAAAGAAAG -3'

Sequencing Primer
(F):5'- GCCAAGACCCAAACAAGTGTTATG -3'
(R):5'- CGCCAAAGAAAGGCACTATAAAG -3'

Posted On

2013-04-16