Mutagenetix > Incidental Mutation

Incidental Mutation 'R2567:Cd209d'

254555

Institutional Source

Beutler Lab

Gene Symbol

Cd209d

Ensembl Gene

ENSMUSG00000031495

Gene Name

CD209d antigen

Synonyms

SIGNR3, mSIGNR3, SIGN-R3

MMRRC Submission

040426-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2567 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

3921824-3928548 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 3926327 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 96 (N96I)

Ref Sequence

ENSEMBL: ENSMUSP00000147198 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000011445] [ENSMUST00000209176]

AlphaFold

Q91ZW8

Predicted Effect

probably damaging
Transcript: ENSMUST00000011445
AA Change: N126I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000011445
Gene: ENSMUSG00000031495
AA Change: N126I

Domain	Start	End	E-Value	Type
low complexity region	54	74	N/A	INTRINSIC
CLECT	106	227	2.34e-34	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000209176
AA Change: N96I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal susceptibility to bacterial infection despite decreased T and B cell proliferation and extramedullary hematopoiesis in the spleen. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm1	A	G	3: 59,836,475 (GRCm39)		probably benign	Het
Aebp1	A	G	11: 5,820,251 (GRCm39)	D409G	probably benign	Het
Akap10	A	G	11: 61,784,175 (GRCm39)		probably benign	Het
Akap6	G	T	12: 52,985,156 (GRCm39)	S863I	probably damaging	Het
Ap1s3	T	C	1: 79,602,921 (GRCm39)	K29E	possibly damaging	Het
Atm	T	A	9: 53,368,770 (GRCm39)	I2341L	possibly damaging	Het
Atp12a	A	T	14: 56,624,384 (GRCm39)	D944V	probably damaging	Het
Baz2b	A	T	2: 59,744,255 (GRCm39)	S1417T	possibly damaging	Het
Cacna1a	T	A	8: 85,276,354 (GRCm39)	M613K	probably damaging	Het
Ccdc191	A	C	16: 43,764,330 (GRCm39)		probably null	Het
Cdh15	G	A	8: 123,588,763 (GRCm39)	R279Q	probably damaging	Het
Cdk4	T	G	10: 126,900,145 (GRCm39)	V14G	probably benign	Het
Chrna10	C	A	7: 101,761,276 (GRCm39)	M438I	probably benign	Het
Clec10a	T	C	11: 70,060,358 (GRCm39)		probably null	Het
Cog3	A	G	14: 75,991,730 (GRCm39)	V40A	probably benign	Het
Creb3l3	T	C	10: 80,921,883 (GRCm39)	H315R	probably benign	Het
Csmd3	CCTTTGCGCTT	CCTT	15: 47,604,632 (GRCm39)		probably null	Het
Cubn	A	G	2: 13,283,167 (GRCm39)		probably null	Het
Cygb	T	C	11: 116,540,692 (GRCm39)	D98G	probably damaging	Het
Dmbx1	T	C	4: 115,777,489 (GRCm39)	K120E	probably damaging	Het
Dnah3	T	A	7: 119,551,920 (GRCm39)	I2800F	possibly damaging	Het
Dntt	G	T	19: 41,029,775 (GRCm39)	R245L	possibly damaging	Het
Dock1	T	G	7: 134,747,213 (GRCm39)	V1508G	probably damaging	Het
Enpp4	A	C	17: 44,412,736 (GRCm39)	I266R	probably damaging	Het
Fhl4	T	C	10: 84,934,644 (GRCm39)	I46V	possibly damaging	Het
Fn1	G	A	1: 71,636,895 (GRCm39)	Q1995*	probably null	Het
Foxl3	A	G	5: 138,805,940 (GRCm39)	S36G	probably null	Het
Foxo1	T	A	3: 52,176,755 (GRCm39)	L178H	probably damaging	Het
Galnt18	C	T	7: 111,153,823 (GRCm39)	R267H	probably damaging	Het
Gm1110	T	C	9: 26,831,992 (GRCm39)	D53G	probably benign	Het
Gm7104	A	T	12: 88,252,242 (GRCm39)		noncoding transcript	Het
H2-M11	C	T	17: 36,859,042 (GRCm39)	T194I	possibly damaging	Het
Haus6	C	A	4: 86,504,122 (GRCm39)	E501*	probably null	Het
Ifna5	T	C	4: 88,754,147 (GRCm39)	V129A	probably benign	Het
Kdelr3	A	G	15: 79,407,032 (GRCm39)	I38V	probably benign	Het
Lamb1	A	G	12: 31,319,054 (GRCm39)		probably null	Het
Larp7-ps	T	C	4: 92,079,560 (GRCm39)	E87G	probably benign	Het
Mgat4c	T	A	10: 102,214,123 (GRCm39)	F35L	probably benign	Het
Mmab	A	T	5: 114,571,378 (GRCm39)	M166K	probably benign	Het
Mrpl2	A	G	17: 46,958,427 (GRCm39)	T70A	probably benign	Het
Naa11	C	T	5: 97,539,618 (GRCm39)	G180D	probably benign	Het
Npy6r	T	C	18: 44,408,888 (GRCm39)	V103A	possibly damaging	Het
Nup188	A	T	2: 30,231,794 (GRCm39)	R1463W	possibly damaging	Het
Nusap1	T	A	2: 119,474,311 (GRCm39)	S336R	possibly damaging	Het
Or8b12c	T	A	9: 37,715,509 (GRCm39)	F101I	probably damaging	Het
Pabpc4	T	A	4: 123,191,744 (GRCm39)	L589Q	probably damaging	Het
Pcdh12	T	A	18: 38,415,149 (GRCm39)	N659Y	probably damaging	Het
Perm1	T	C	4: 156,301,575 (GRCm39)	S40P	probably damaging	Het
Phldb1	T	C	9: 44,637,322 (GRCm39)	T114A	probably damaging	Het
Pirt	C	T	11: 66,816,985 (GRCm39)	L99F	probably damaging	Het
Plxdc2	A	G	2: 16,716,995 (GRCm39)	R360G	probably benign	Het
Rhpn2	G	A	7: 35,080,957 (GRCm39)		probably null	Het
Rpusd2	T	A	2: 118,867,556 (GRCm39)	I268N	probably damaging	Het
Sds	G	T	5: 120,619,646 (GRCm39)	W185L	probably damaging	Het
Serpinb5	A	G	1: 106,802,876 (GRCm39)	K137R	probably benign	Het
Sh3pxd2a	A	G	19: 47,413,008 (GRCm39)	V25A	possibly damaging	Het
Slc1a2	C	T	2: 102,597,355 (GRCm39)	T454I	probably damaging	Het
Slc25a40	T	C	5: 8,480,459 (GRCm39)	C70R	probably damaging	Het
Smoc1	G	A	12: 81,214,364 (GRCm39)	E260K	probably damaging	Het
Sparcl1	A	T	5: 104,232,954 (GRCm39)	F616I	probably damaging	Het
Ssc5d	A	T	7: 4,939,334 (GRCm39)	D590V	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Trpm2	C	T	10: 77,777,008 (GRCm39)	V430M	probably damaging	Het
Ttn	T	C	2: 76,574,672 (GRCm39)	D25407G	probably damaging	Het
Vmn2r68	T	C	7: 84,883,803 (GRCm39)	I101V	probably benign	Het
Xrcc4	A	T	13: 90,210,261 (GRCm39)	M61K	probably damaging	Het
Zfp648	A	G	1: 154,080,695 (GRCm39)	T285A	probably damaging	Het

Other mutations in Cd209d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01634:Cd209d	APN	8	3,927,974 (GRCm39)	splice site	probably null
IGL01707:Cd209d	APN	8	3,928,296 (GRCm39)	missense	probably benign
IGL02864:Cd209d	APN	8	3,927,122 (GRCm39)	missense	probably benign	0.08
IGL03066:Cd209d	APN	8	3,928,437 (GRCm39)	critical splice donor site	probably null
IGL03297:Cd209d	APN	8	3,928,476 (GRCm39)	missense	possibly damaging	0.92
R0324:Cd209d	UTSW	8	3,928,258 (GRCm39)	missense	probably benign	0.31
R1335:Cd209d	UTSW	8	3,922,027 (GRCm39)	missense	probably damaging	1.00
R1349:Cd209d	UTSW	8	3,928,515 (GRCm39)	unclassified	probably benign
R1372:Cd209d	UTSW	8	3,928,515 (GRCm39)	unclassified	probably benign
R1507:Cd209d	UTSW	8	3,928,453 (GRCm39)	missense	possibly damaging	0.72
R1673:Cd209d	UTSW	8	3,927,113 (GRCm39)	missense	probably damaging	1.00
R2393:Cd209d	UTSW	8	3,928,436 (GRCm39)	critical splice donor site	probably null
R4907:Cd209d	UTSW	8	3,927,948 (GRCm39)	missense	probably benign	0.01
R5349:Cd209d	UTSW	8	3,928,320 (GRCm39)	missense	probably benign	0.00
R5768:Cd209d	UTSW	8	3,921,968 (GRCm39)	missense	probably benign	0.05
R5949:Cd209d	UTSW	8	3,927,949 (GRCm39)	missense	possibly damaging	0.50
R5953:Cd209d	UTSW	8	3,927,979 (GRCm39)	splice site	probably null
R6103:Cd209d	UTSW	8	3,928,304 (GRCm39)	missense	probably damaging	1.00
R7382:Cd209d	UTSW	8	3,927,965 (GRCm39)	nonsense	probably null
R8714:Cd209d	UTSW	8	3,923,772 (GRCm39)	frame shift	probably null
R8715:Cd209d	UTSW	8	3,923,772 (GRCm39)	frame shift	probably null
R8716:Cd209d	UTSW	8	3,923,772 (GRCm39)	frame shift	probably null
R9672:Cd209d	UTSW	8	3,922,036 (GRCm39)	missense	probably damaging	1.00
R9784:Cd209d	UTSW	8	3,926,337 (GRCm39)	missense	probably damaging	1.00
X0025:Cd209d	UTSW	8	3,927,961 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGCAGCATCTAACTCATAGCC -3'
(R):5'- GTCCTGACTTTTGTGCCGAAC -3'

Sequencing Primer
(F):5'- AGCCTTGTCTAGCAAATGATCTC -3'
(R):5'- GCTCCCCAGAATGCAGTGATAAG -3'

Posted On

2014-12-04