Mutagenetix > Incidental Mutation

Incidental Mutation 'R2915:Zfp853'

254837

Institutional Source

Beutler Lab

Gene Symbol

Zfp853

Ensembl Gene

ENSMUSG00000093910

Gene Name

zinc finger protein 853

Synonyms

LOC330230

MMRRC Submission

040502-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.245) question?

Stock #

R2915 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

143287038-143293623 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 143289577 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 96 (E96G)

Ref Sequence

ENSEMBL: ENSMUSP00000148846 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000180336] [ENSMUST00000212355] [ENSMUST00000212715]

AlphaFold

A0A1D5RM95

Predicted Effect

unknown
Transcript: ENSMUST00000180336
AA Change: E111G

SMART Domains

Protein: ENSMUSP00000137494
Gene: ENSMUSG00000093910
AA Change: E111G

Domain	Start	End	E-Value	Type
low complexity region	43	62	N/A	INTRINSIC
low complexity region	80	87	N/A	INTRINSIC
low complexity region	102	145	N/A	INTRINSIC
coiled coil region	280	408	N/A	INTRINSIC
low complexity region	412	424	N/A	INTRINSIC
low complexity region	435	458	N/A	INTRINSIC
low complexity region	488	499	N/A	INTRINSIC
ZnF_C2H2	501	523	7.78e-3	SMART
ZnF_C2H2	529	551	4.87e-4	SMART
ZnF_C2H2	557	579	2.57e-3	SMART
ZnF_C2H2	585	607	2.24e-3	SMART
ZnF_C2H2	613	636	4.17e-3	SMART
low complexity region	639	651	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000212355
AA Change: E111G

Predicted Effect

unknown
Transcript: ENSMUST00000212715
AA Change: E96G

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aif1	G	A	17: 35,172,151	P44L	probably benign	Het
Arhgap11a	A	G	2: 113,833,508	V810A	probably damaging	Het
B3gnt6	T	C	7: 98,193,593	N387D	probably benign	Het
Col5a2	C	G	1: 45,413,496	G358R	probably damaging	Het
Cracr2a	A	G	6: 127,611,505	K209R	probably damaging	Het
Dmkn	A	G	7: 30,765,316	N32S	unknown	Het
Dusp13	T	A	14: 21,740,137	N47I	probably damaging	Het
Elmo2	A	G	2: 165,297,653		probably benign	Het
Ephb6	T	C	6: 41,614,238	F110L	probably damaging	Het
Gabrb2	T	A	11: 42,591,907	N197K	probably benign	Het
Gdnf	T	A	15: 7,815,649	V41E	possibly damaging	Het
Gm21915	A	G	9: 40,670,787	I59V	possibly damaging	Het
Gprin2	C	T	14: 34,195,081	G244D	possibly damaging	Het
Grin2d	T	C	7: 45,833,357		probably benign	Het
Ice2	A	G	9: 69,410,840	D241G	probably benign	Het
Mios	C	T	6: 8,214,935	R44C	possibly damaging	Het
Nlrp5-ps	T	C	7: 14,586,711		noncoding transcript	Het
Nyap2	C	T	1: 81,087,471	R67*	probably null	Het
Olfr1031	T	A	2: 85,992,045	V76E	probably damaging	Het
Olfr1094	T	C	2: 86,829,226	I158T	probably benign	Het
Olfr1306	G	T	2: 111,912,719	D70E	probably damaging	Het
Olfr1428	G	A	19: 12,108,625	P81L	probably benign	Het
Olfr27	G	T	9: 39,144,466	R122L	possibly damaging	Het
Otop2	G	A	11: 115,329,146	A271T	probably benign	Het
Otulin	A	G	15: 27,619,630		probably benign	Het
Pax1	A	G	2: 147,368,428	Y361C	probably damaging	Het
Pcdhb12	A	T	18: 37,437,640	N613I	probably damaging	Het
Plekha5	A	G	6: 140,589,199	K173E	probably damaging	Het
Plin4	T	A	17: 56,104,389	T881S	probably damaging	Het
Poli	A	G	18: 70,522,700		probably null	Het
Prex2	T	A	1: 11,169,853	F898I	probably damaging	Het
Prr14l	A	T	5: 32,829,768	H794Q	probably benign	Het
Prss1	A	T	6: 41,462,611	I93F	probably benign	Het
Ptpro	C	T	6: 137,414,241		probably benign	Het
Rad1	T	C	15: 10,486,642	C42R	probably damaging	Het
Rnf20	A	G	4: 49,638,769	E197G	probably benign	Het
Setx	A	G	2: 29,172,324	E2260G	probably damaging	Het
Sgk1	C	T	10: 21,996,601	R171W	probably damaging	Het
Six2	A	G	17: 85,685,188	S296P	probably damaging	Het
Smg1	T	A	7: 118,210,879		probably benign	Het
Spred2	T	C	11: 19,998,215	V41A	probably damaging	Het
Ssu2	A	T	6: 112,377,605	C219*	probably null	Het
Tbc1d9b	T	A	11: 50,149,736	V360D	possibly damaging	Het
Tdrd9	G	A	12: 112,040,461	D920N	probably damaging	Het
Tyrp1	A	G	4: 80,837,455	T154A	possibly damaging	Het
Vrk3	C	T	7: 44,775,442	T427M	probably benign	Het
Wac	A	C	18: 7,926,131	M596L	possibly damaging	Het
Zfhx2	G	T	14: 55,064,557	P1990Q	probably damaging	Het
Zzef1	G	A	11: 72,910,326		probably null	Het

Other mutations in Zfp853

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1656:Zfp853	UTSW	5	143289085	splice site	probably benign
R2072:Zfp853	UTSW	5	143289382	missense	unknown
R4862:Zfp853	UTSW	5	143289661	missense	unknown
R4869:Zfp853	UTSW	5	143288293	missense	probably damaging	0.99
R4945:Zfp853	UTSW	5	143288829	missense	unknown
R5139:Zfp853	UTSW	5	143288815	missense	unknown
R5335:Zfp853	UTSW	5	143288563	missense	unknown
R5426:Zfp853	UTSW	5	143288869	missense	unknown
R5844:Zfp853	UTSW	5	143288669	missense	unknown
R5845:Zfp853	UTSW	5	143288669	missense	unknown
R5847:Zfp853	UTSW	5	143288669	missense	unknown
R6039:Zfp853	UTSW	5	143288774	nonsense	probably null
R6039:Zfp853	UTSW	5	143288774	nonsense	probably null
R7124:Zfp853	UTSW	5	143289607	missense	unknown
R7283:Zfp853	UTSW	5	143287738	missense	unknown
R7323:Zfp853	UTSW	5	143289355	missense	unknown
R8026:Zfp853	UTSW	5	143288525	missense	unknown
R8121:Zfp853	UTSW	5	143288263	missense	probably damaging	0.99
R8290:Zfp853	UTSW	5	143289071	nonsense	probably null
R8347:Zfp853	UTSW	5	143288947	missense	unknown
R9017:Zfp853	UTSW	5	143288488	missense	unknown
R9110:Zfp853	UTSW	5	143289565	missense	unknown
R9123:Zfp853	UTSW	5	143288741	nonsense	probably null
R9560:Zfp853	UTSW	5	143289325	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AGCTGCATCATCTGGTGCTG -3'
(R):5'- CCTGCCATTTCCACTACAGGTG -3'

Sequencing Primer
(F):5'- AAATGTTGCTCTTGTCCAAGTC -3'
(R):5'- CACTACAGGTGGCAGTGGTG -3'

Posted On

2014-12-29