|Institutional Source||Beutler Lab|
|Gene Name||vaccinia related kinase 3|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R2915 (G1)|
|Chromosomal Location||44748413-44777515 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 44775442 bp (GRCm38)|
|Amino Acid Change||Threonine to Methionine at position 427 (T427M)|
|Ref Sequence||ENSEMBL: ENSMUSP00000002275 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000002275] [ENSMUST00000147952]|
AA Change: T427M
PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
AA Change: T427M
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||98% (50/51)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele display impaired social behavior, decreased fear and anxiety relate behavior, impaired spatial memory, and abnormal hippocampal dendritic spine and synapse morphologies. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Vrk3||
(F):5'- TTGATGCTTCCCACACCGAC -3'
(R):5'- AGATCACCCAGCTGAATTCC -3'
(F):5'- CAGCAGATACAAGAACTCCCAGTG -3'
(R):5'- CAGCTCTTGAATTGACTTCAGG -3'