Mutagenetix > Incidental Mutation

Incidental Mutation 'R2973:Mroh9'

255259

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

040526-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2973 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

162851871-162913239 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 162884338 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 400 (M400K)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

probably damaging
Transcript: ENSMUST00000096608
AA Change: M400K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: M400K

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000104420

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195132

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	T	G	10: 79,844,801 (GRCm39)	F1508V	probably damaging	Het
Angptl7	T	A	4: 148,584,671 (GRCm39)	K26*	probably null	Het
Aoc1l1	A	G	6: 48,953,358 (GRCm39)	T428A	probably benign	Het
BC048507	T	C	13: 68,011,749 (GRCm39)	I42T	probably benign	Het
Cald1	A	G	6: 34,734,931 (GRCm39)		probably benign	Het
Ces5a	A	G	8: 94,255,132 (GRCm39)	F187S	probably damaging	Het
Chd3	A	G	11: 69,251,442 (GRCm39)	Y530H	probably damaging	Het
Crnkl1	A	G	2: 145,774,181 (GRCm39)	L94P	probably benign	Het
Cst9	G	T	2: 148,677,145 (GRCm39)		probably null	Het
Cstdc2	T	C	2: 148,692,706 (GRCm39)	D32G	probably benign	Het
D930007J09Rik	C	T	13: 32,986,742 (GRCm39)		probably benign	Het
Dcaf10	T	C	4: 45,373,957 (GRCm39)	S461P	probably benign	Het
Dhx36	T	A	3: 62,402,916 (GRCm39)	N279Y	probably benign	Het
Dhx36	C	G	3: 62,402,919 (GRCm39)	G278R	possibly damaging	Het
Eps8l3	T	C	3: 107,798,644 (GRCm39)	I510T	probably damaging	Het
Gab2	A	G	7: 96,872,759 (GRCm39)	E54G	probably benign	Het
Gsdme	A	G	6: 50,206,304 (GRCm39)	C180R	probably damaging	Het
Hesx1	A	G	14: 26,722,599 (GRCm39)		probably benign	Het
Il5ra	G	A	6: 106,718,196 (GRCm39)	P86L	probably benign	Het
Jakmip1	A	G	5: 37,248,871 (GRCm39)	K177R	probably damaging	Het
Larp4b	T	C	13: 9,216,347 (GRCm39)		probably benign	Het
Lrrc42	A	T	4: 107,096,311 (GRCm39)	D40E	probably damaging	Het
Mefv	G	A	16: 3,533,558 (GRCm39)	R238*	probably null	Het
Or12j2	T	G	7: 139,916,300 (GRCm39)	F175C	probably damaging	Het
Or5ac22	A	C	16: 59,135,767 (GRCm39)	M1R	probably null	Het
Or5d37	A	G	2: 87,923,458 (GRCm39)	L274P	probably benign	Het
Plec	C	G	15: 76,072,961 (GRCm39)	G631R	probably damaging	Het
Polr3b	A	G	10: 84,464,144 (GRCm39)	K35E	probably benign	Het
Ppef2	T	C	5: 92,386,953 (GRCm39)	E328G	probably benign	Het
Rogdi	G	T	16: 4,829,526 (GRCm39)	Q90K	probably damaging	Het
Rtn1	C	A	12: 72,270,163 (GRCm39)	R116L	probably damaging	Het
Septin14	T	C	5: 129,776,086 (GRCm39)	H31R	probably benign	Het
Serpinb9e	T	A	13: 33,439,126 (GRCm39)	V184E	probably benign	Het
Slc10a5	A	T	3: 10,399,517 (GRCm39)	I381N	probably damaging	Het
Slit1	G	A	19: 41,599,455 (GRCm39)	P1032L	probably benign	Het
Sptlc3	A	G	2: 139,431,581 (GRCm39)	T368A	probably damaging	Het
Tm9sf1	T	A	14: 55,878,571 (GRCm39)	T274S	probably benign	Het
Tpgs1	A	G	10: 79,505,449 (GRCm39)	E69G	probably damaging	Het
Trpv5	T	C	6: 41,630,165 (GRCm39)	S642G	possibly damaging	Het
Ttn	A	C	2: 76,632,661 (GRCm39)	I12385S	probably damaging	Het
Ube2e2	T	C	14: 18,630,321 (GRCm38)	D137G	possibly damaging	Het
Ubr4	T	C	4: 139,133,847 (GRCm39)	Y748H	probably benign	Het
Ugt8a	T	C	3: 125,708,957 (GRCm39)	H51R	probably benign	Het
Vcp	T	A	4: 42,996,315 (GRCm39)	I16F	probably damaging	Het
Vmn2r117	A	G	17: 23,678,830 (GRCm39)	V798A	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	162,873,350 (GRCm39)	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	162,907,072 (GRCm39)	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	162,852,227 (GRCm39)	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	162,888,191 (GRCm39)	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	162,866,700 (GRCm39)	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	162,875,435 (GRCm39)	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	162,908,144 (GRCm39)	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	162,883,172 (GRCm39)	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	162,875,551 (GRCm39)	splice site	probably null
IGL01823:Mroh9	APN	1	162,883,178 (GRCm39)	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	162,890,071 (GRCm39)	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	162,885,648 (GRCm39)	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	162,903,149 (GRCm39)	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	162,908,145 (GRCm39)	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	162,852,205 (GRCm39)	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	162,854,071 (GRCm39)	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	162,866,766 (GRCm39)	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	162,888,331 (GRCm39)	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0629:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	162,893,693 (GRCm39)	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	162,871,552 (GRCm39)	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	162,854,078 (GRCm39)	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	162,852,110 (GRCm39)	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	162,852,161 (GRCm39)	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	162,884,347 (GRCm39)	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	162,873,279 (GRCm39)	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	162,866,714 (GRCm39)	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	162,854,082 (GRCm39)	missense	probably damaging	0.97
R1923:Mroh9	UTSW	1	162,903,860 (GRCm39)	missense	probably damaging	1.00
R2325:Mroh9	UTSW	1	162,854,099 (GRCm39)	splice site	probably null
R2511:Mroh9	UTSW	1	162,866,514 (GRCm39)	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	162,884,341 (GRCm39)	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	162,893,638 (GRCm39)	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	162,908,122 (GRCm39)	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	162,871,662 (GRCm39)	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	162,893,593 (GRCm39)	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	162,883,162 (GRCm39)	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	162,852,061 (GRCm39)	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	162,854,093 (GRCm39)	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5157:Mroh9	UTSW	1	162,871,690 (GRCm39)	missense	probably damaging	0.96
R5324:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5325:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	162,908,156 (GRCm39)	start gained	probably benign
R6005:Mroh9	UTSW	1	162,903,246 (GRCm39)	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	162,893,612 (GRCm39)	nonsense	probably null
R6414:Mroh9	UTSW	1	162,902,271 (GRCm39)	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	162,903,873 (GRCm39)	missense	probably damaging	1.00
R6540:Mroh9	UTSW	1	162,866,541 (GRCm39)	missense	possibly damaging	0.87
R6541:Mroh9	UTSW	1	162,885,607 (GRCm39)	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	162,903,130 (GRCm39)	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	162,873,610 (GRCm39)	missense	possibly damaging	0.86
R6830:Mroh9	UTSW	1	162,903,935 (GRCm39)	missense	probably benign
R7026:Mroh9	UTSW	1	162,888,251 (GRCm39)	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	162,866,525 (GRCm39)	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	162,866,750 (GRCm39)	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	162,903,858 (GRCm39)	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	162,902,277 (GRCm39)	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	162,873,601 (GRCm39)	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	162,852,122 (GRCm39)	missense	probably benign
R7808:Mroh9	UTSW	1	162,866,678 (GRCm39)	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	162,866,802 (GRCm39)	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	162,866,544 (GRCm39)	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	162,890,096 (GRCm39)	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	162,852,294 (GRCm39)	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	162,883,250 (GRCm39)	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	162,899,595 (GRCm39)	splice site	probably null
R8960:Mroh9	UTSW	1	162,883,196 (GRCm39)	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	162,890,069 (GRCm39)	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	162,875,412 (GRCm39)	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	162,890,030 (GRCm39)	missense
R9596:Mroh9	UTSW	1	162,893,576 (GRCm39)	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	162,866,498 (GRCm39)	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	162,885,630 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGAGTGCCATGTCCAACTTACC -3'
(R):5'- ACCTTGTGTTTGCAGAACTCTG -3'

Sequencing Primer
(F):5'- CCCAACATGTACAGCTATATATTTCC -3'
(R):5'- CAGAACTCTGCTACTGCTGTGG -3'

Posted On

2014-12-29