Mutagenetix > Incidental Mutation

Incidental Mutation 'R2919:Mslnl'

255449

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040504-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2919 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 95.9%

Validation Efficiency

98% (53/54)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001J03Rik	C	T	5: 146,185,191	R27H	probably benign	Het
2310002L09Rik	A	T	4: 73,950,608	L31Q	probably damaging	Het
Agbl1	G	A	7: 76,414,658	D53N	probably damaging	Het
Aloxe3	C	T	11: 69,142,923	T621I	probably damaging	Het
Als2cl	A	T	9: 110,897,499		probably null	Het
Atg9b	T	A	5: 24,391,544	T125S	possibly damaging	Het
Casp8ap2	A	G	4: 32,645,343	D1472G	probably damaging	Het
Cd1d2	C	G	3: 86,987,680	P158A	probably damaging	Het
Cdc45	A	G	16: 18,808,793	I94T	probably benign	Het
Chd6	T	G	2: 160,967,880	D1487A	possibly damaging	Het
Edem2	T	C	2: 155,709,027	Y340C	probably damaging	Het
Fbxl12	C	T	9: 20,642,213	R26H	probably damaging	Het
Fgfr3	A	G	5: 33,733,940	N516S	probably damaging	Het
Gm10093	A	G	17: 78,492,846	D422G	probably damaging	Het
Gm5884	A	G	6: 128,645,058		noncoding transcript	Het
Inpp4b	C	A	8: 81,985,329	A425E	possibly damaging	Het
Kif1a	A	G	1: 93,046,742	Y964H	probably damaging	Het
Lrp1b	A	C	2: 41,770,899	C66G	probably damaging	Het
Lrp4	A	G	2: 91,490,730	I1034V	probably benign	Het
Mmrn2	T	C	14: 34,402,922	V820A	possibly damaging	Het
Mphosph9	G	A	5: 124,261,006	T982I	probably benign	Het
Mroh9	A	C	1: 163,056,772	M399R	probably damaging	Het
N4bp2	G	A	5: 65,807,098	G830D	probably benign	Het
Nagpa	G	A	16: 5,203,787		probably benign	Het
Olfr1000	G	A	2: 85,608,410	P167S	probably benign	Het
Olfr690	C	T	7: 105,329,860	V111M	probably damaging	Het
Oxgr1	T	A	14: 120,022,809		probably benign	Het
Parp3	T	A	9: 106,473,725	R323W	possibly damaging	Het
Pfkfb3	C	T	2: 11,484,327	V286I	probably benign	Het
Pfkp	C	T	13: 6,593,243	G513D	probably damaging	Het
Pla2g4d	T	C	2: 120,281,627		probably benign	Het
Rtl1	T	C	12: 109,591,148	E1419G	unknown	Het
Sdf2	G	C	11: 78,254,854	V126L	probably damaging	Het
Sgk2	T	G	2: 162,999,195	L175R	probably damaging	Het
Sgk2	C	A	2: 162,999,205	F178L	probably damaging	Het
Sim1	T	C	10: 50,909,815	Y255H	probably benign	Het
Slc13a5	T	C	11: 72,247,791	E442G	possibly damaging	Het
Slc38a3	A	T	9: 107,657,687	I163N	probably damaging	Het
Slc39a4	A	G	15: 76,616,670	L31P	probably damaging	Het
Slc5a11	GGTGC	G	7: 123,239,372		probably null	Het
Slfnl1	G	T	4: 120,533,078		probably benign	Het
Stradb	G	A	1: 58,992,669	V247M	probably benign	Het
Styk1	T	A	6: 131,313,004		probably benign	Het
Sult1d1	A	G	5: 87,559,755		probably benign	Het
Syk	C	T	13: 52,611,121	P95S	probably benign	Het
Taar2	T	A	10: 23,941,556	N331K	probably benign	Het
Thsd7b	A	G	1: 130,189,850		probably benign	Het
Tldc1	G	A	8: 119,768,317	A234V	probably benign	Het
Tmbim7	G	A	5: 3,673,188		probably null	Het
Tmem107	T	C	11: 69,071,421	L68P	probably damaging	Het
Tmem184c	A	T	8: 77,604,647	C158S	probably damaging	Het
Tmpo	A	G	10: 91,152,686	I310T	probably benign	Het
Ugt2b5	A	G	5: 87,125,407	F467L	possibly damaging	Het
Xirp1	T	C	9: 120,018,701	E372G	possibly damaging	Het
Zbtb5	T	C	4: 44,994,790	E198G	probably damaging	Het
Zfp804a	A	G	2: 82,235,816	N44D	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TGCCATCAGAAGGGAGGTTTG -3'
(R):5'- CACAGGTCTGAAGCACTGAG -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2014-12-29