Incidental Mutation 'R3012:Il15'
ID 257537
Institutional Source Beutler Lab
Gene Symbol Il15
Ensembl Gene ENSMUSG00000031712
Gene Name interleukin 15
Synonyms
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R3012 (G1)
Quality Score 225
Status Not validated
Chromosome 8
Chromosomal Location 83058261-83129851 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 83071049 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Isoleucine at position 22 (N22I)
Ref Sequence ENSEMBL: ENSMUSP00000147312 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034148] [ENSMUST00000209363] [ENSMUST00000209573] [ENSMUST00000210094] [ENSMUST00000211565]
AlphaFold P48346
Predicted Effect probably damaging
Transcript: ENSMUST00000034148
AA Change: N22I

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000034148
Gene: ENSMUSG00000031712
AA Change: N22I

DomainStartEndE-ValueType
Pfam:IL15 33 160 8.6e-53 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000209363
AA Change: N22I

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
Predicted Effect probably damaging
Transcript: ENSMUST00000209573
AA Change: N22I

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000209687
Predicted Effect probably damaging
Transcript: ENSMUST00000210094
AA Change: N22I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000210885
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211282
Predicted Effect probably damaging
Transcript: ENSMUST00000211565
AA Change: N22I

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211722
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.1%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a a pleiotropic cytokine of the interleukin family of proteins that plays important roles in the innate and adaptive cell homeostasis, as well as peripheral immune function. The encoded protein undergoes proteolytic processing to generate a mature cytokine that stimulates the proliferation of natural killer cells. The transgenic mice overexpressing the encoded protein exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking the encoded protein exhibit impaired protection against a strain of attenuated Mycobacterium. [provided by RefSeq, Aug 2016]
PHENOTYPE: Mice homozygous for disruptions of this gene have normal life spans but display a variety of immune system abnormalities and maternal placental defects. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 26 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aldh18a1 G T 19: 40,546,135 (GRCm39) Y585* probably null Het
Ankrd17 G A 5: 90,378,727 (GRCm39) P2563S probably damaging Het
Atg10 A T 13: 91,302,397 (GRCm39) F47Y probably damaging Het
Ccdc39 C T 3: 33,868,817 (GRCm39) R798Q probably damaging Het
Cfi A G 3: 129,668,579 (GRCm39) D535G probably damaging Het
Dennd11 T C 6: 40,412,926 (GRCm39) E45G probably benign Het
Dgat1 T A 15: 76,387,593 (GRCm39) Q308H possibly damaging Het
Dsp T C 13: 38,377,318 (GRCm39) I1701T possibly damaging Het
Egr2 GAA GA 10: 67,375,733 (GRCm39) probably null Het
Fes G C 7: 80,036,915 (GRCm39) S56R possibly damaging Het
Gria1 G A 11: 57,180,260 (GRCm39) V737M probably damaging Het
Gtf2h1 A G 7: 46,453,319 (GRCm39) H84R probably damaging Het
Ifi208 A G 1: 173,523,136 (GRCm39) probably null Het
Me1 A G 9: 86,493,965 (GRCm39) S323P probably benign Het
Or10a5 T C 7: 106,635,569 (GRCm39) F69S possibly damaging Het
Parp4 T C 14: 56,832,873 (GRCm39) probably null Het
Pcdhga7 A G 18: 37,848,691 (GRCm39) T233A probably benign Het
Rab36 G A 10: 74,880,328 (GRCm39) V63I probably damaging Het
Rpe65 G T 3: 159,310,200 (GRCm39) V128F possibly damaging Het
Slc5a11 GGTGC G 7: 122,838,595 (GRCm39) probably null Het
Tbc1d32 A G 10: 56,050,011 (GRCm39) V509A probably benign Het
Tln1 T A 4: 43,542,525 (GRCm39) T1428S probably benign Het
Ttc27 A T 17: 75,147,454 (GRCm39) I669F probably benign Het
Tubb3 T G 8: 124,147,975 (GRCm39) C303G probably damaging Het
Tulp2 G T 7: 45,168,187 (GRCm39) V188L probably damaging Het
Zfr T C 15: 12,166,249 (GRCm39) Y840H probably damaging Het
Other mutations in Il15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02590:Il15 APN 8 83,069,912 (GRCm39) missense probably benign
R0306:Il15 UTSW 8 83,061,083 (GRCm39) splice site probably benign
R0638:Il15 UTSW 8 83,069,890 (GRCm39) missense probably damaging 0.99
R0685:Il15 UTSW 8 83,064,188 (GRCm39) splice site probably benign
R7089:Il15 UTSW 8 83,064,204 (GRCm39) missense probably damaging 0.98
R9499:Il15 UTSW 8 83,061,177 (GRCm39) missense probably benign 0.00
R9551:Il15 UTSW 8 83,061,177 (GRCm39) missense probably benign 0.00
R9552:Il15 UTSW 8 83,061,177 (GRCm39) missense probably benign 0.00
R9674:Il15 UTSW 8 83,069,938 (GRCm39) missense probably damaging 0.98
R9679:Il15 UTSW 8 83,071,094 (GRCm39) missense probably benign 0.05
R9720:Il15 UTSW 8 83,058,608 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- TTAAGGATTTGCACGTCCCC -3'
(R):5'- GAGCCACTGAAATTTCACTGTTTCC -3'

Sequencing Primer
(F):5'- CCTGATATCCTACGCTGAAGAGTG -3'
(R):5'- GATTGTGAAATGACACCTTTAC -3'
Posted On 2015-01-11