Mutagenetix > Incidental Mutation

Incidental Mutation 'R3014:Vmn2r13'

257592

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r13

Ensembl Gene

ENSMUSG00000091635

Gene Name

vomeronasal 2, receptor 13

Synonyms

Gm4867

MMRRC Submission

040535-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R3014 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109303889-109339973 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 109319627 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 451 (H451L)

Ref Sequence

ENSEMBL: ENSMUSP00000052977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053253]

AlphaFold

L7N1X2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000053253
AA Change: H451L

PolyPhen 2 Score 0.810 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000052977
Gene: ENSMUSG00000091635
AA Change: H451L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	76	463	2.8e-29	PFAM
Pfam:NCD3G	506	560	1.3e-18	PFAM
Pfam:7tm_3	593	828	1.8e-54	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.5%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Angpt4	G	T	2: 151,771,517 (GRCm39)	V278L	probably benign	Het
Dmbt1	A	G	7: 130,633,827 (GRCm39)		probably benign	Het
Dnah2	A	T	11: 69,321,304 (GRCm39)	N3921K	probably benign	Het
Duxf4	G	A	10: 58,071,356 (GRCm39)	T286I	possibly damaging	Het
Fam193a	A	T	5: 34,623,016 (GRCm39)	E1201D	probably benign	Het
Gm10220	G	C	5: 26,322,826 (GRCm39)	D195E	probably damaging	Het
Gmps	A	G	3: 63,922,857 (GRCm39)	K607E	possibly damaging	Het
Gprin1	T	A	13: 54,886,288 (GRCm39)	D662V	probably benign	Het
Gria4	A	G	9: 4,464,294 (GRCm39)	I556T	probably damaging	Het
Grm7	G	T	6: 110,623,309 (GRCm39)	V161F	probably damaging	Het
Hcar2	A	T	5: 124,002,768 (GRCm39)	V245E	probably damaging	Het
Hmces	T	G	6: 87,894,823 (GRCm39)	D62E	probably benign	Het
Ighv1-4	T	A	12: 114,450,769 (GRCm39)	Y113F	possibly damaging	Het
Kcnd3	C	T	3: 105,566,082 (GRCm39)	A421V	probably damaging	Het
Kng1	C	T	16: 22,898,120 (GRCm39)	H507Y	possibly damaging	Het
Lypd10	A	G	7: 24,412,821 (GRCm39)	I76M	possibly damaging	Het
Map3k5	A	T	10: 19,970,175 (GRCm39)	Y694F	probably damaging	Het
Myo1a	T	G	10: 127,552,214 (GRCm39)	F778V	probably damaging	Het
Naip2	A	C	13: 100,298,290 (GRCm39)	I582S	probably benign	Het
Or1ad1	A	G	11: 50,875,534 (GRCm39)	D2G	probably benign	Het
Or4l1	G	A	14: 50,166,489 (GRCm39)	H171Y	probably benign	Het
Or6aa1	A	G	7: 86,043,884 (GRCm39)	V274A	probably benign	Het
Or6z6	A	T	7: 6,491,470 (GRCm39)	Y134*	probably null	Het
Parp3	C	A	9: 106,348,514 (GRCm39)	S507I	possibly damaging	Het
Ppip5k2	T	A	1: 97,671,800 (GRCm39)	T491S	probably damaging	Het
Psmg1	T	A	16: 95,781,248 (GRCm39)	I266F	probably damaging	Het
Ptpn23	T	C	9: 110,218,763 (GRCm39)	T476A	probably benign	Het
Rttn	G	C	18: 89,032,744 (GRCm39)	V671L	probably damaging	Het
Srsf7	T	C	17: 80,508,990 (GRCm39)	E235G	unknown	Het
Sv2a	G	A	3: 96,096,751 (GRCm39)	W416*	probably null	Het
Tas2r107	A	G	6: 131,636,972 (GRCm39)	Y26H	probably benign	Het
Tenm2	T	C	11: 35,914,800 (GRCm39)	T2245A	probably damaging	Het
Xkr6	T	C	14: 64,056,793 (GRCm39)	Y235H	unknown	Het

Other mutations in Vmn2r13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Vmn2r13	APN	5	109,303,964 (GRCm39)	missense	probably damaging	1.00
IGL01373:Vmn2r13	APN	5	109,304,568 (GRCm39)	missense	probably damaging	1.00
IGL01946:Vmn2r13	APN	5	109,322,085 (GRCm39)	missense	probably benign	0.01
IGL01971:Vmn2r13	APN	5	109,321,981 (GRCm39)	missense	probably benign	0.01
IGL02636:Vmn2r13	APN	5	109,339,883 (GRCm39)	missense	probably damaging	0.98
IGL03062:Vmn2r13	APN	5	109,304,148 (GRCm39)	missense	probably damaging	1.00
IGL03173:Vmn2r13	APN	5	109,319,645 (GRCm39)	missense	possibly damaging	0.95
IGL03301:Vmn2r13	APN	5	109,305,955 (GRCm39)	missense	probably damaging	0.99
IGL03383:Vmn2r13	APN	5	109,304,398 (GRCm39)	missense	probably damaging	0.98
IGL03048:Vmn2r13	UTSW	5	109,304,151 (GRCm39)	missense	probably damaging	1.00
R0123:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R0134:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R0220:Vmn2r13	UTSW	5	109,304,332 (GRCm39)	missense	probably damaging	1.00
R0225:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R0393:Vmn2r13	UTSW	5	109,304,395 (GRCm39)	missense	probably benign	0.01
R0410:Vmn2r13	UTSW	5	109,321,679 (GRCm39)	missense	probably benign	0.35
R0787:Vmn2r13	UTSW	5	109,304,713 (GRCm39)	missense	probably damaging	0.99
R1200:Vmn2r13	UTSW	5	109,322,068 (GRCm39)	missense	probably damaging	1.00
R1448:Vmn2r13	UTSW	5	109,322,001 (GRCm39)	missense	probably damaging	1.00
R1782:Vmn2r13	UTSW	5	109,306,040 (GRCm39)	missense	probably benign	0.08
R1939:Vmn2r13	UTSW	5	109,339,852 (GRCm39)	missense	possibly damaging	0.88
R2029:Vmn2r13	UTSW	5	109,339,943 (GRCm39)	missense	probably benign	0.13
R2125:Vmn2r13	UTSW	5	109,306,058 (GRCm39)	missense	probably benign	0.00
R2126:Vmn2r13	UTSW	5	109,306,058 (GRCm39)	missense	probably benign	0.00
R2379:Vmn2r13	UTSW	5	109,319,644 (GRCm39)	missense	probably benign	0.05
R2680:Vmn2r13	UTSW	5	109,322,178 (GRCm39)	missense	possibly damaging	0.66
R2888:Vmn2r13	UTSW	5	109,339,840 (GRCm39)	missense	possibly damaging	0.88
R2889:Vmn2r13	UTSW	5	109,339,840 (GRCm39)	missense	possibly damaging	0.88
R2890:Vmn2r13	UTSW	5	109,339,840 (GRCm39)	missense	possibly damaging	0.88
R3683:Vmn2r13	UTSW	5	109,304,721 (GRCm39)	missense	probably damaging	1.00
R4074:Vmn2r13	UTSW	5	109,304,566 (GRCm39)	missense	probably damaging	1.00
R4599:Vmn2r13	UTSW	5	109,304,322 (GRCm39)	missense	probably damaging	1.00
R4614:Vmn2r13	UTSW	5	109,323,065 (GRCm39)	missense	probably benign	0.01
R4805:Vmn2r13	UTSW	5	109,304,331 (GRCm39)	missense	probably damaging	1.00
R4822:Vmn2r13	UTSW	5	109,321,938 (GRCm39)	missense	probably damaging	0.99
R4943:Vmn2r13	UTSW	5	109,322,915 (GRCm39)	missense	probably benign	0.00
R5263:Vmn2r13	UTSW	5	109,321,841 (GRCm39)	missense	probably benign	0.00
R5297:Vmn2r13	UTSW	5	109,339,805 (GRCm39)	missense	probably benign	0.00
R5502:Vmn2r13	UTSW	5	109,321,580 (GRCm39)	missense	probably damaging	1.00
R5554:Vmn2r13	UTSW	5	109,339,860 (GRCm39)	missense	possibly damaging	0.49
R5563:Vmn2r13	UTSW	5	109,321,846 (GRCm39)	missense	probably benign	0.00
R5819:Vmn2r13	UTSW	5	109,321,966 (GRCm39)	missense	possibly damaging	0.79
R6074:Vmn2r13	UTSW	5	109,322,167 (GRCm39)	missense	probably benign	0.04
R6416:Vmn2r13	UTSW	5	109,321,982 (GRCm39)	missense	probably damaging	0.99
R6419:Vmn2r13	UTSW	5	109,323,085 (GRCm39)	missense	possibly damaging	0.87
R6484:Vmn2r13	UTSW	5	109,304,540 (GRCm39)	nonsense	probably null
R6486:Vmn2r13	UTSW	5	109,304,425 (GRCm39)	missense	probably benign	0.05
R6545:Vmn2r13	UTSW	5	109,304,806 (GRCm39)	splice site	probably null
R6700:Vmn2r13	UTSW	5	109,322,938 (GRCm39)	missense	probably benign	0.00
R6897:Vmn2r13	UTSW	5	109,306,015 (GRCm39)	missense	possibly damaging	0.90
R6957:Vmn2r13	UTSW	5	109,304,753 (GRCm39)	nonsense	probably null
R7276:Vmn2r13	UTSW	5	109,321,645 (GRCm39)	missense	probably damaging	1.00
R7363:Vmn2r13	UTSW	5	109,339,909 (GRCm39)	missense	probably benign	0.03
R7443:Vmn2r13	UTSW	5	109,339,909 (GRCm39)	missense	probably benign	0.03
R7555:Vmn2r13	UTSW	5	109,319,557 (GRCm39)	splice site	probably null
R7607:Vmn2r13	UTSW	5	109,321,506 (GRCm39)	missense	probably damaging	0.98
R7719:Vmn2r13	UTSW	5	109,319,618 (GRCm39)	missense	probably benign	0.00
R8116:Vmn2r13	UTSW	5	109,322,926 (GRCm39)	missense	probably benign	0.12
R8242:Vmn2r13	UTSW	5	109,322,872 (GRCm39)	missense	possibly damaging	0.65
R8294:Vmn2r13	UTSW	5	109,322,978 (GRCm39)	missense	probably benign	0.02
R8340:Vmn2r13	UTSW	5	109,322,006 (GRCm39)	missense	probably benign	0.00
R8692:Vmn2r13	UTSW	5	109,319,514 (GRCm39)	missense	probably benign	0.03
R8742:Vmn2r13	UTSW	5	109,304,263 (GRCm39)	missense	probably benign	0.02
R9022:Vmn2r13	UTSW	5	109,304,242 (GRCm39)	missense	possibly damaging	0.94
R9281:Vmn2r13	UTSW	5	109,303,953 (GRCm39)	missense	probably damaging	1.00
R9529:Vmn2r13	UTSW	5	109,304,064 (GRCm39)	missense	probably damaging	1.00
R9708:Vmn2r13	UTSW	5	109,322,007 (GRCm39)	missense	probably benign	0.00
R9746:Vmn2r13	UTSW	5	109,339,773 (GRCm39)	critical splice donor site	probably null
X0066:Vmn2r13	UTSW	5	109,304,085 (GRCm39)	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- CCCAGAATTATTACTCATGTGCCTC -3'
(R):5'- ACGAGGGATTACTTTAATACAGCTG -3'

Sequencing Primer
(F):5'- ACTCTGGTGGAAACAAGG -3'
(R):5'- GTGTGCATGTGTAAATGTCT -3'

Posted On

2015-01-11